Rembert Pieper

Summary

Affiliation: J. Craig Venter Institute
Country: USA

Publications

  1. pmc Characterizing the Escherichia coli O157:H7 proteome including protein associations with higher order assemblies
    Rembert Pieper
    J Craig Venter Institute, Rockville, Maryland, United States of America
    PLoS ONE 6:e26554. 2011
  2. pmc Development stage-specific proteomic profiling uncovers small, lineage specific proteins most abundant in the Aspergillus Fumigatus conidial proteome
    Moo Jin Suh
    The J, Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, USA
    Proteome Sci 10:30. 2012
  3. pmc Integral and peripheral association of proteins and protein complexes with Yersinia pestis inner and outer membranes
    Rembert Pieper
    Craig Venter Institute, 9704 Medical Center Drive, Rockville, Maryland, USA
    Proteome Sci 7:5. 2009
  4. doi request reprint Temperature and growth phase influence the outer-membrane proteome and the expression of a type VI secretion system in Yersinia pestis
    Rembert Pieper
    J Craig Venter Institute, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Microbiology 155:498-512. 2009
  5. doi request reprint Characterizing the dynamic nature of the Yersinia pestis periplasmic proteome in response to nutrient exhaustion and temperature change
    Rembert Pieper
    J Craig Venter Institute, Rockville, MD 20850, USA
    Proteomics 8:1442-58. 2008
  6. pmc Comparison of two label-free global quantitation methods, APEX and 2D gel electrophoresis, applied to the Shigella dysenteriae proteome
    Srilatha Kuntumalla
    Pathogen Functional Genomics Resource Center, J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Proteome Sci 7:22. 2009
  7. pmc The Shigella dysenteriae serotype 1 proteome, profiled in the host intestinal environment, reveals major metabolic modifications and increased expression of invasive proteins
    Rembert Pieper
    Pathogen Functional Genomics Resource Center, J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Proteomics 9:5029-45. 2009
  8. pmc Proteomic analysis of iron acquisition, metabolic and regulatory responses of Yersinia pestis to iron starvation
    Rembert Pieper
    J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    BMC Microbiol 10:30. 2010
  9. pmc In vivo versus in vitro protein abundance analysis of Shigella dysenteriae type 1 reveals changes in the expression of proteins involved in virulence, stress and energy metabolism
    Srilatha Kuntumalla
    Pathogen Functional Genomics Resource Center, J, Craig Venter Institute, Rockville, MD 20850, USA
    BMC Microbiol 11:147. 2011
  10. pmc Recombinant expression and functional analysis of proteases from Streptococcus pneumoniae, Bacillus anthracis, and Yersinia pestis
    Keehwan Kwon
    Pathogen Functional Genomics Resource Center, J, Craig Venter Institute, Rockville, Maryland 20850, USA
    BMC Biochem 12:17. 2011

Detail Information

Publications23

  1. pmc Characterizing the Escherichia coli O157:H7 proteome including protein associations with higher order assemblies
    Rembert Pieper
    J Craig Venter Institute, Rockville, Maryland, United States of America
    PLoS ONE 6:e26554. 2011
    ....
  2. pmc Development stage-specific proteomic profiling uncovers small, lineage specific proteins most abundant in the Aspergillus Fumigatus conidial proteome
    Moo Jin Suh
    The J, Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, USA
    Proteome Sci 10:30. 2012
    ..abstract:..
  3. pmc Integral and peripheral association of proteins and protein complexes with Yersinia pestis inner and outer membranes
    Rembert Pieper
    Craig Venter Institute, 9704 Medical Center Drive, Rockville, Maryland, USA
    Proteome Sci 7:5. 2009
    ..The total number of proteins associated with Y. pestis membranes increased to 456 and included representatives of all six beta-barrel OM protein families and 25 distinct IM transporter families...
  4. doi request reprint Temperature and growth phase influence the outer-membrane proteome and the expression of a type VI secretion system in Yersinia pestis
    Rembert Pieper
    J Craig Venter Institute, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Microbiology 155:498-512. 2009
    ..pestis cells with trypsin. Proteases and other stress-response-inducing factors may constitute environmental cues resulting in the activation of the T6SS in Y. pestis...
  5. doi request reprint Characterizing the dynamic nature of the Yersinia pestis periplasmic proteome in response to nutrient exhaustion and temperature change
    Rembert Pieper
    J Craig Venter Institute, Rockville, MD 20850, USA
    Proteomics 8:1442-58. 2008
    ..pestis life cycle were strongly altered in abundance. This included a putative nitrate/sulfonate/bicarbonate-specific SBP (Y1004), encoded by the virulence-associated plasmid pMT1 and increased in abundance at 37 degrees C...
  6. pmc Comparison of two label-free global quantitation methods, APEX and 2D gel electrophoresis, applied to the Shigella dysenteriae proteome
    Srilatha Kuntumalla
    Pathogen Functional Genomics Resource Center, J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Proteome Sci 7:22. 2009
    ..A high correlation was observed for subunits of soluble cellular protein complexes in several cases, demonstrating versatile applications of the APEX method in quantitative proteomics...
  7. pmc The Shigella dysenteriae serotype 1 proteome, profiled in the host intestinal environment, reveals major metabolic modifications and increased expression of invasive proteins
    Rembert Pieper
    Pathogen Functional Genomics Resource Center, J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Proteomics 9:5029-45. 2009
    ..The outer membrane protein OmpA, the heat shock protein HtpG and OspC2 represent novel SD1 subunit vaccine candidates and drug targets...
  8. pmc Proteomic analysis of iron acquisition, metabolic and regulatory responses of Yersinia pestis to iron starvation
    Rembert Pieper
    J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    BMC Microbiol 10:30. 2010
    ..pestis strain KIM6+ at two physiologically relevant temperatures (26 degrees C and 37 degrees C)...
  9. pmc In vivo versus in vitro protein abundance analysis of Shigella dysenteriae type 1 reveals changes in the expression of proteins involved in virulence, stress and energy metabolism
    Srilatha Kuntumalla
    Pathogen Functional Genomics Resource Center, J, Craig Venter Institute, Rockville, MD 20850, USA
    BMC Microbiol 11:147. 2011
    ....
  10. pmc Recombinant expression and functional analysis of proteases from Streptococcus pneumoniae, Bacillus anthracis, and Yersinia pestis
    Keehwan Kwon
    Pathogen Functional Genomics Resource Center, J, Craig Venter Institute, Rockville, Maryland 20850, USA
    BMC Biochem 12:17. 2011
    ..It has been observed that cloning, expression and purification of proteases often fail due to their catalytic functions which, in turn, cause toxicity in the E. coli heterologous host...
  11. pmc Using chemical derivatization and mass spectrometric analysis to characterize the post-translationally modified Staphylococcus aureus surface protein G
    Moo Jin Suh
    Pathogen Functional Genomics Resource Center, J Craig Venter Institute, Rockville, MD 20850, USA
    Biochim Biophys Acta 1804:1394-404. 2010
    ....
  12. ncbi request reprint Proteomic profiling of cell envelope-associated proteins from Staphylococcus aureus
    Christine L Gatlin
    The Institute for Genomic Research, Rockville, MD, USA
    Proteomics 6:1530-49. 2006
    ....
  13. ncbi request reprint Comparative proteomic analysis of Staphylococcus aureus strains with differences in resistance to the cell wall-targeting antibiotic vancomycin
    Rembert Pieper
    The Institute for Genomic Research, Rockville, MD, USA
    Proteomics 6:4246-58. 2006
    ....
  14. pmc High quality protein microarray using in situ protein purification
    Keehwan Kwon
    Pathogen Functional Genomics Resource Center, J Craig Venter Institute, 9704 Medical Center Drive, Rockville, Maryland 20850, USA
    BMC Biotechnol 9:72. 2009
    ..Optimized in situ purification of His-tagged recombinant proteins has the potential to become the new gold standard for cost-effective generation of high-quality and high-density protein microarrays...
  15. pmc Analysis of the proteome of intracellular Shigella flexneri reveals pathways important for intracellular growth
    Rembert Pieper
    J Craig Venter Institute, Rockville, Maryland, USA
    Infect Immun 81:4635-48. 2013
    ..flexneri. Thus, iron stress and changes in carbon metabolism may be key factors in the S. flexneri transition from the extra- to the intracellular milieu. ..
  16. pmc A proteogenomic update to Yersinia: enhancing genome annotation
    Samuel H Payne
    J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    BMC Genomics 11:460. 2010
    ..The resulting gene set contains numerous errors in even the most basic form of annotation: the primary structure of the proteins...
  17. pmc The APEX Quantitative Proteomics Tool: generating protein quantitation estimates from LC-MS/MS proteomics results
    John C Braisted
    Pathogen Functional Genomics Resource Center, J Craig Venter Institute, Rockville, MD 20850, USA
    BMC Bioinformatics 9:529. 2008
    ..This predicted spectral count is compared to the protein's observed MS total spectral count during APEX computation of protein abundances...
  18. ncbi request reprint A correlation analysis of protein characteristics associated with genome-wide high throughput expression and solubility of Streptococcus pneumoniae proteins
    Keehwan Kwon
    J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Protein Expr Purif 55:368-78. 2007
    ..Given a relatively large dataset derived from proteins representing all functional groups in a microbial genome we correlated various protein characteristics as they relate to protein expression outcomes...
  19. pmc Integrated next-generation sequencing of 16S rDNA and metaproteomics differentiate the healthy urine microbiome from asymptomatic bacteriuria in neuropathic bladder associated with spinal cord injury
    Derrick E Fouts
    J Craig Venter Institute, Rockville, MD 20850, USA
    J Transl Med 10:174. 2012
    ....
  20. doi request reprint Preparation of urine samples for proteomic analysis
    Rembert Pieper
    The Institute for Genomic Research, Rockville, Maryland, USA
    Methods Mol Biol 425:89-99. 2008
    ....
  21. ncbi request reprint Genome transplantation in bacteria: changing one species to another
    Carole Lartigue
    J Craig Venter Institute, Rockville, MD 20850, USA
    Science 317:632-8. 2007
    ..These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria...
  22. ncbi request reprint Multi-component immunoaffinity subtraction chromatography: an innovative step towards a comprehensive survey of the human plasma proteome
    Rembert Pieper
    Large Scale Biology Corporation, Germantown, Maryland, USA
    Proteomics 3:422-32. 2003
    ..This facilitates automated chromatographic processing of plasma samples in high throughput, which is desirable in proteomic disease marker discovery projects...