ROGER SENDER LASKEN

Summary

Affiliation: J. Craig Venter Institute
Country: USA

Publications

  1. ncbi request reprint Single-cell genomic sequencing using Multiple Displacement Amplification
    Roger S Lasken
    J Craig Venter Institute, 11149 N Torrey Pines Rd, Suite 200, La Jolla, CA 92037, USA
    Curr Opin Microbiol 10:510-6. 2007
  2. pmc Genomic DNA amplification from a single bacterium
    Arumugham Raghunathan
    Molecular Staging, Inc, New Haven, Connecticut, USA
    Appl Environ Microbiol 71:3342-7. 2005
  3. ncbi request reprint Specific single-cell isolation and genomic amplification of uncultured microorganisms
    Thomas Kvist
    Environmental Microbiology and Biotechnology Group, BioCentrum Technical University of Denmark, Building 227, Lyngby, Denmark
    Appl Microbiol Biotechnol 74:926-35. 2007
  4. pmc Mechanism of chimera formation during the Multiple Displacement Amplification reaction
    Roger S Lasken
    J Craig Venter Institute, Rockville, MD 20850, USA
    BMC Biotechnol 7:19. 2007
  5. pmc Genomic sequencing of single microbial cells from environmental samples
    Thomas Ishoey
    J Craig Venter Institute, 10355 Science Center Drive, San Diego, CA 92121, United States
    Curr Opin Microbiol 11:198-204. 2008
  6. pmc Efficient de novo assembly of single-cell bacterial genomes from short-read data sets
    Hamidreza Chitsaz
    Department of Computer Science, University of California, La Jolla, CA, USA
    Nat Biotechnol 29:915-21. 2011
  7. doi request reprint Genomic sequencing of uncultured microorganisms from single cells
    Roger S Lasken
    J Craig Venter Institute, La Jolla, California 92121, USA
    Nat Rev Microbiol 10:631-40. 2012
  8. pmc Multiple displacement amplification as an adjunct to PCR-based detection of Staphylococcus aureus in synovial fluid
    Sandeep Kathju
    Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA, USA
    BMC Res Notes 3:259. 2010
  9. doi request reprint Genomic DNA amplification by the multiple displacement amplification (MDA) method
    Roger S Lasken
    J Craig Venter Institute, La Jolla, CA 92121, USA
    Biochem Soc Trans 37:450-3. 2009
  10. ncbi request reprint Something from (almost) nothing: the impact of multiple displacement amplification on microbial ecology
    Erik K Binga
    Department of Biology, University of Waterloo, Waterloo, Ontario, Canada
    ISME J 2:233-41. 2008

Research Grants

Collaborators

  • Josh D Neufeld
  • Jonathan H Badger
  • Ole Schulz-Trieglaff
  • Christopher L Dupont
  • Hamidreza Chitsaz
  • Marc Mussmann
  • Ramunas Stepanauskas
  • A Fawad Faruqi
  • Seiyu Hosono
  • Sandeep Kathju
  • Wanmin Song
  • Michael Egholm
  • Zhenyu Sun
  • Xiaohong Wu
  • Yuefen Du
  • Jing Du
  • Frank B Dean
  • Erik K Binga
  • Thomas Ishoey
  • Thomas Kvist
  • Traci Kiesling
  • Arumugham Raghunathan
  • David L Barker
  • Carole J Bornarth
  • Mark Driscoll
  • Osama A Alsmadi
  • Stephen F Kingsmore
  • Patrick G Buckley
  • Paul Stoodley
  • Latha Satish
  • Garth D Ehrlich
  • Sandra Johnson
  • J Christopher Post
  • Tanja Woyke
  • Mark Novotny
  • Evan Skowronski
  • Mark Danielsen
  • Guy Grater
  • Kenneth Dretchen
  • Peter Westermann
  • Birgitte K Ahring
  • Jonathan Leshin
  • Eugene A Davidson
  • Shannon Hibbard
  • Kendra Cox
  • Harley R Ferguson
  • Jonathon H Pinter
  • Martin Latterich
  • Diane Giannola
  • Eric Lai
  • Vladimir Makarov
  • Richard Shen
  • Irina Sleptsova
  • Mark S T Hansen
  • William Ziehler
  • Arnold Oliphant
  • Orlando R Irsula
  • Joel P Brockman
  • Michele Wisniewski
  • Mark D Driscoll
  • PATRICIO ABARZUA
  • Elisabeth Blennow
  • David M Beare
  • Carina Hirvelä
  • Isabel Tapia-Paez
  • Carl E G Bruder
  • Charlotte Thyr
  • Kiran K Mantripragada
  • Anna Wedell
  • Britt Inger Johnsson
  • Haider Ali
  • Patricia Bray-Ward
  • Koichi Ichimura
  • Boris C Bastian
  • Ingegerd Fransson
  • Magdalena Benetkiewicz
  • Ian Dunham
  • John E Collins
  • Birgitta Sinder Wilén
  • Magnus Rosenquist
  • Linhua Fang
  • Uwe Menzel
  • V Peter Collins
  • Qiuling Zong
  • Caroline Jarbo
  • Daniel Pinkel
  • Nils Mandahl
  • Jan P Dumanski
  • Martin Hergersberg
  • Teresita Díaz de Ståhl

Detail Information

Publications18

  1. ncbi request reprint Single-cell genomic sequencing using Multiple Displacement Amplification
    Roger S Lasken
    J Craig Venter Institute, 11149 N Torrey Pines Rd, Suite 200, La Jolla, CA 92037, USA
    Curr Opin Microbiol 10:510-6. 2007
    ..The genetically linked sequences from single cells are also a powerful tool to be used in guiding genomic assembly of shotgun sequences of multiple organisms from environmental DNA extracts (metagenomic sequences)...
  2. pmc Genomic DNA amplification from a single bacterium
    Arumugham Raghunathan
    Molecular Staging, Inc, New Haven, Connecticut, USA
    Appl Environ Microbiol 71:3342-7. 2005
    ..A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying non-culturable microorganisms...
  3. ncbi request reprint Specific single-cell isolation and genomic amplification of uncultured microorganisms
    Thomas Kvist
    Environmental Microbiology and Biotechnology Group, BioCentrum Technical University of Denmark, Building 227, Lyngby, Denmark
    Appl Microbiol Biotechnol 74:926-35. 2007
    ..The system was validated using Methanothermobacter thermoautotrophicus as single-cell test organism, and the validation setup produced 100% sequence homology to the ten tested regions of the genome of this organism...
  4. pmc Mechanism of chimera formation during the Multiple Displacement Amplification reaction
    Roger S Lasken
    J Craig Venter Institute, Rockville, MD 20850, USA
    BMC Biotechnol 7:19. 2007
    ..Determining the source of the DNA rearrangements would be an important step towards reducing or eliminating them...
  5. pmc Genomic sequencing of single microbial cells from environmental samples
    Thomas Ishoey
    J Craig Venter Institute, 10355 Science Center Drive, San Diego, CA 92121, United States
    Curr Opin Microbiol 11:198-204. 2008
    ..Methods are reviewed for preparation of bacterial fractions from environmental samples, single-cell isolation, DNA amplification by MDA, and DNA sequencing...
  6. pmc Efficient de novo assembly of single-cell bacterial genomes from short-read data sets
    Hamidreza Chitsaz
    Department of Computer Science, University of California, La Jolla, CA, USA
    Nat Biotechnol 29:915-21. 2011
    ..Our approach enables acquisition of genome assemblies for individual uncultivated bacteria using only short reads, providing cell-specific genetic information absent from metagenomic studies...
  7. doi request reprint Genomic sequencing of uncultured microorganisms from single cells
    Roger S Lasken
    J Craig Venter Institute, La Jolla, California 92121, USA
    Nat Rev Microbiol 10:631-40. 2012
    ..Here, the technical developments that have enabled single-cell sequencing, as well as some of the most exciting applications of this approach from the past few years, are reviewed...
  8. pmc Multiple displacement amplification as an adjunct to PCR-based detection of Staphylococcus aureus in synovial fluid
    Sandeep Kathju
    Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA, USA
    BMC Res Notes 3:259. 2010
    ..aureus DNA as well as on clinical samples known to contain S. aureus nucleic acids...
  9. doi request reprint Genomic DNA amplification by the multiple displacement amplification (MDA) method
    Roger S Lasken
    J Craig Venter Institute, La Jolla, CA 92121, USA
    Biochem Soc Trans 37:450-3. 2009
    ..Remarkably, MDA enables genomic sequencing even from single microbial cells. Some of the uses of MDA and its strengths and limitations will be discussed...
  10. ncbi request reprint Something from (almost) nothing: the impact of multiple displacement amplification on microbial ecology
    Erik K Binga
    Department of Biology, University of Waterloo, Waterloo, Ontario, Canada
    ISME J 2:233-41. 2008
    ..Whole genome amplification via MDA has increased access to the genomic DNA of uncultivated microorganisms and low-biomass environments and represents a 'power tool' in the molecular toolbox of microbial ecologists...
  11. pmc Sequence specific detection of DNA using nicking endonuclease signal amplification (NESA)
    Traci Kiesling
    Department of Biochemistry and Molecular Biology and Cellular Biology, Georgetown University School of Medicine, Washington, DC 20057, USA
    Nucleic Acids Res 35:e117. 2007
    ..This method should be applicable whenever there is a requirement to detect a specific DNA sequence. Other applications include SNP analysis and genotyping. The reaction is inherently simple to multiplex and is amenable to automation...
  12. pmc Insights into the genome of large sulfur bacteria revealed by analysis of single filaments
    Marc Mussmann
    Max Planck Institute for Marine Microbiology, Bremen, Germany
    PLoS Biol 5:e230. 2007
    ..The first look into the genome of these filamentous sulfur-oxidizing bacteria substantially deepens the understanding of their evolution and their contribution to sulfur and nitrogen cycling in marine sediments...
  13. pmc Two methods of whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel
    David L Barker
    Illumina, Inc, San Diego, California 92121, USA
    Genome Res 14:901-7. 2004
    ..The combination of whole-genome amplification and comprehensive SNP linkage analysis offers new opportunities for genetic analysis in clinical trials, disease association studies, and archiving of DNA samples...
  14. ncbi request reprint Whole genome amplification: abundant supplies of DNA from precious samples or clinical specimens
    Roger S Lasken
    Molecular Staging Inc, 300 George Street, New Haven, CT 06511, USA
    Trends Biotechnol 21:531-5. 2003
  15. pmc High accuracy genotyping directly from genomic DNA using a rolling circle amplification based assay
    Osama A Alsmadi
    Molecular Staging, Inc, 300 George Street, Suite 701, New Haven, CT 06511, USA
    BMC Genomics 4:21. 2003
    ..The low cost and scalability of ligation/ERCA genotyping makes it ideally suited for automated, high throughput methods...
  16. pmc Unbiased whole-genome amplification directly from clinical samples
    Seiyu Hosono
    Molecular Staging, Inc, New Haven, Connecticut 06511, USA
    Genome Res 13:954-64. 2003
    ..The low amplification bias of MDA represents a dramatic technical improvement in the ability to amplify a whole genome compared with older, PCR-based methods...
  17. ncbi request reprint A full-coverage, high-resolution human chromosome 22 genomic microarray for clinical and research applications
    Patrick G Buckley
    Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, 751 85 Uppsala, Sweden
    Hum Mol Genet 11:3221-9. 2002
    ..Furthermore, comprehensive epigenetic profiling of 22q-located genes and high-resolution analysis of replication timing across the entire chromosome can be studied using our array...
  18. pmc Comprehensive human genome amplification using multiple displacement amplification
    Frank B Dean
    Molecular Staging, Inc, 300 George Street, Suite 701, New Haven, CT 06511, USA
    Proc Natl Acad Sci U S A 99:5261-6. 2002
    ..MDA-based WGA is a simple and reliable method that could have significant implications for genetic studies, forensics, diagnostics, and long-term sample storage...

Research Grants4

  1. WHOLE GENOME AMPLIFICATION FOR RAPID LOW COST GENOTYPING
    ROGER LASKEN; Fiscal Year: 2005
    ..C) Individual lymphocytes will be used as a model system to prove feasibility of doing preimplantation genetic diagnosis from single blastomeres. ..
  2. WHOLE GENOME AMPLIFICATION FOR RAPID LOW COST GENOTYPING
    ROGER LASKEN; Fiscal Year: 2005
    ..C) Individual lymphocytes will be used as a model system to prove feasibility of doing preimplantation genetic diagnosis from single blastomeres. ..
  3. Single Cell Genomics of Human Microbial Flora
    ROGER LASKEN; Fiscal Year: 2009
    ....
  4. Single Cell Genomics of Human Microbial Flora
    ROGER SENDER LASKEN; Fiscal Year: 2010
    ....