Affiliation: J. Craig Venter Institute
- Targeted chromosomal knockouts in Mycoplasma pneumoniaeRadha Krishnakumar
J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
Appl Environ Microbiol 76:5297-9. 2010..Following selection for the antibiotic resistance, colonies are screened for double crossovers which indicate complete deletion of the target open reading frame...
- Experimental challenges of sense codon reassignment: an innovative approach to genetic code expansionRadha Krishnakumar
Synthetic Biology and Bioenergy, J Craig Venter Institute, Rockville, MD 20850, USA Electronic address
FEBS Lett 588:383-8. 2014....
- Creation of a bacterial cell controlled by a chemically synthesized genomeDaniel G Gibson
The J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
Science 329:52-6. 2010..The new cells have expected phenotypic properties and are capable of continuous self-replication...
- Transfer RNA misidentification scrambles sense codon recodingRadha Krishnakumar
Synthetic Biology and Bioenergy, J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850 USA
Chembiochem 14:1967-72. 2013..This result is not unexpected as most tRNA synthetases use the anticodon as a recognition element. The data suggest that tRNA misidentification by endogenous aminoacyl-tRNA synthetases needs to be overcome for sense codon recoding...