Research Topics
| Clyde A HutchisonSummaryAffiliation: J. Craig Venter Institute Country: USA Publications
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Detail Information
Publications
Cell-free cloning using phi29 DNA polymeraseClyde A Hutchison
Synthetic Biology Group, The J Craig Venter Institute, Rockville, MD 20850, USA
Proc Natl Acad Sci U S A 102:17332-6. 2005..These include environmental DNA samples that have proven difficult to clone and synthetic genes encoding toxic products. The method may also speed genome sequencing by eliminating the need for biological cloning...
Creation of a bacterial cell controlled by a chemically synthesized genomeDaniel G Gibson
The J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
Science 329:52-6. 2010..The new cells have expected phenotypic properties and are capable of continuous self-replication...- Cloning whole bacterial genomes in yeastGwynedd A Benders
Synthetic Biology and Bioenergy Group, The J Craig Venter Institute, San Diego, CA 92121, USA
Nucleic Acids Res 38:2558-69. 2010..6 Mb), M. pneumoniae (0.8 Mb) and M. mycoides subspecies capri (1.1 Mb) as yeast circular centromeric plasmids. These genomes appear to be stably maintained in a host that has efficient, well-established methods for DNA manipulation... - Creating bacterial strains from genomes that have been cloned and engineered in yeastCarole Lartigue
J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
Science 325:1693-6. 2009..mycoides. These methods allow the construction of strains that could not be produced with genetic tools available for this bacterium... - One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genomeDaniel G Gibson
The J Craig Venter Institute, Synthetic Biology Group, Rockville, MD 20850, USA
Proc Natl Acad Sci U S A 105:20404-9. 2008..The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments... - Enzymatic assembly of DNA molecules up to several hundred kilobasesDaniel G Gibson
The J Craig Venter Institute, Synthetic Biology Group, Rockville, Maryland, USA
Nat Methods 6:343-5. 2009..This assembly method can be used to seamlessly construct synthetic and natural genes, genetic pathways and entire genomes, and could be a useful molecular engineering tool... - Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genomeDaniel G Gibson
J Craig Venter Institute, Rockville, MD 20850, USA
Science 319:1215-20. 2008..The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments... 
Genome transplantation in bacteria: changing one species to anotherCarole Lartigue
J Craig Venter Institute, Rockville, MD 20850, USA
Science 317:632-8. 2007..These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria...- Chemical synthesis of the mouse mitochondrial genomeDaniel G Gibson
The J Craig Venter Institute, Rockville, Maryland, USA
Nat Methods 7:901-3. 2010..As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers... - Sequence analysis of a complete 1.66 Mb Prochlorococcus marinus MED4 genome cloned in yeastChristian Tagwerker
Department of Synthetic Biology and Bioenergy, J Craig Venter Institute, 10355 Science Center Drive, San Diego, CA 92121, USA
Nucleic Acids Res 40:10375-83. 2012..We thus provide evidence of transformation, replication and maintenance of this 1.66 Mb intact bacterial genome in S. cerevisiae... - Essential genes of a minimal bacteriumJohn I Glass
Synthetic Biology Group, J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
Proc Natl Acad Sci U S A 103:425-30. 2006..Genes encoding proteins of unknown function constitute 28% of the essential protein-coding genes set. Disruption of some genes accelerated M. genitalium growth... - Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotidesHamilton O Smith
Institute for Biological Energy Alternatives, 1901 Research Boulevard, Suite 600, Rockville, MD 20850, USA
Proc Natl Acad Sci U S A 100:15440-5. 2003..One such isolate had exactly the intended sequence. We propose to assemble larger genomes by joining separately assembled 5- to 6-kb segments; approximately 60 such segments would be required for a minimal cellular genome... - DNA sequencing: bench to bedside and beyondClyde A Hutchison
J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
Nucleic Acids Res 35:6227-37. 2007..These advances will also allow new approaches to a variety of problems in biology, evolution and the environment... - Single-cell genomicsClyde A Hutchison
Nat Biotechnol 24:657-8. 2006 - Similarity-based gene detection: using COGs to find evolutionarily-conserved ORFsBradford C Powell
Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina, USA
BMC Bioinformatics 7:31. 2006..Although COG analysis was initially developed to group annotated genes, we applied it to the task of grouping anonymous DNA sequences that may encode proteins...