Clyde A Hutchison

Summary

Affiliation: J. Craig Venter Institute
Country: USA

Publications

  1. pmc DNA sequencing: bench to bedside and beyond
    Clyde A Hutchison
    J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 35:6227-37. 2007
  2. doi request reprint Creation of a bacterial cell controlled by a chemically synthesized genome
    Daniel G Gibson
    The J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Science 329:52-6. 2010
  3. pmc One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 105:20404-9. 2008
  4. doi request reprint Creating bacterial strains from genomes that have been cloned and engineered in yeast
    Carole Lartigue
    J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Science 325:1693-6. 2009
  5. doi request reprint Assembly of large, high G+C bacterial DNA fragments in yeast
    Vladimir N Noskov
    Department of Synthetic Biology and Bioenergy, J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    ACS Synth Biol 1:267-73. 2012
  6. doi request reprint Cloning the Acholeplasma laidlawii PG-8A genome in Saccharomyces cerevisiae as a yeast centromeric plasmid
    Bogumil J Karas
    J Craig Venter Institute, 10355 Science Center Drive, San Diego, CA 92121, USA
    ACS Synth Biol 1:22-8. 2012
  7. doi request reprint Transferring whole genomes from bacteria to yeast spheroplasts using entire bacterial cells to reduce DNA shearing
    Bogumil J Karas
    Department of Synthetic Biology and Bioenergy, J Craig Venter Institute, La Jolla, California, USA
    Nat Protoc 9:743-50. 2014
  8. doi request reprint Enzymatic assembly of DNA molecules up to several hundred kilobases
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, Rockville, Maryland, USA
    Nat Methods 6:343-5. 2009
  9. pmc Sequence analysis of a complete 1.66 Mb Prochlorococcus marinus MED4 genome cloned in yeast
    Christian Tagwerker
    Department of Synthetic Biology and Bioenergy, J Craig Venter Institute, 10355 Science Center Drive, San Diego, CA 92121, USA
    Nucleic Acids Res 40:10375-83. 2012
  10. doi request reprint Chemical synthesis of the mouse mitochondrial genome
    Daniel G Gibson
    The J Craig Venter Institute, Rockville, Maryland, USA
    Nat Methods 7:901-3. 2010

Collaborators

Detail Information

Publications18

  1. pmc DNA sequencing: bench to bedside and beyond
    Clyde A Hutchison
    J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 35:6227-37. 2007
    ..These advances will also allow new approaches to a variety of problems in biology, evolution and the environment...
  2. doi request reprint Creation of a bacterial cell controlled by a chemically synthesized genome
    Daniel G Gibson
    The J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Science 329:52-6. 2010
    ..The new cells have expected phenotypic properties and are capable of continuous self-replication...
  3. pmc One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 105:20404-9. 2008
    ..The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments...
  4. doi request reprint Creating bacterial strains from genomes that have been cloned and engineered in yeast
    Carole Lartigue
    J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Science 325:1693-6. 2009
    ..mycoides. These methods allow the construction of strains that could not be produced with genetic tools available for this bacterium...
  5. doi request reprint Assembly of large, high G+C bacterial DNA fragments in yeast
    Vladimir N Noskov
    Department of Synthetic Biology and Bioenergy, J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    ACS Synth Biol 1:267-73. 2012
    ..Thus, cloning strategies utilizing yeast recombination with large, high G+C prokaryotic sequences should include yeast origins of replication as a part of the design process...
  6. doi request reprint Cloning the Acholeplasma laidlawii PG-8A genome in Saccharomyces cerevisiae as a yeast centromeric plasmid
    Bogumil J Karas
    J Craig Venter Institute, 10355 Science Center Drive, San Diego, CA 92121, USA
    ACS Synth Biol 1:22-8. 2012
    ..laidlawii gene, a surface anchored extracellular endonuclease, was toxic when cloned in yeast. This gene was inactivated in order to clone and stably maintain the A. laidlawii genome as a centromeric plasmid in the yeast cell...
  7. doi request reprint Transferring whole genomes from bacteria to yeast spheroplasts using entire bacterial cells to reduce DNA shearing
    Bogumil J Karas
    Department of Synthetic Biology and Bioenergy, J Craig Venter Institute, La Jolla, California, USA
    Nat Protoc 9:743-50. 2014
    ..Excluding the time required for preparing starting cultures and for incubating cells to form final colonies, the protocol can be completed in 3 h. ..
  8. doi request reprint Enzymatic assembly of DNA molecules up to several hundred kilobases
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, Rockville, Maryland, USA
    Nat Methods 6:343-5. 2009
    ..This assembly method can be used to seamlessly construct synthetic and natural genes, genetic pathways and entire genomes, and could be a useful molecular engineering tool...
  9. pmc Sequence analysis of a complete 1.66 Mb Prochlorococcus marinus MED4 genome cloned in yeast
    Christian Tagwerker
    Department of Synthetic Biology and Bioenergy, J Craig Venter Institute, 10355 Science Center Drive, San Diego, CA 92121, USA
    Nucleic Acids Res 40:10375-83. 2012
    ..We thus provide evidence of transformation, replication and maintenance of this 1.66 Mb intact bacterial genome in S. cerevisiae...
  10. doi request reprint Chemical synthesis of the mouse mitochondrial genome
    Daniel G Gibson
    The J Craig Venter Institute, Rockville, Maryland, USA
    Nat Methods 7:901-3. 2010
    ..As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers...
  11. ncbi request reprint Genome transplantation in bacteria: changing one species to another
    Carole Lartigue
    J Craig Venter Institute, Rockville, MD 20850, USA
    Science 317:632-8. 2007
    ..These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria...
  12. pmc Cloning whole bacterial genomes in yeast
    Gwynedd A Benders
    Synthetic Biology and Bioenergy Group, The J Craig Venter Institute, San Diego, CA 92121, USA
    Nucleic Acids Res 38:2558-69. 2010
    ..6 Mb), M. pneumoniae (0.8 Mb) and M. mycoides subspecies capri (1.1 Mb) as yeast circular centromeric plasmids. These genomes appear to be stably maintained in a host that has efficient, well-established methods for DNA manipulation...
  13. pmc Direct transfer of whole genomes from bacteria to yeast
    Bogumil J Karas
    Department of Synthetic Biology and Bioenergy, J Craig Venter Institute, San Diego, California, USA
    Nat Methods 10:410-2. 2013
    ..8 megabases (Mb) into yeast under conditions that promote cell fusion. Moreover, we discovered that removal of restriction endonucleases from donor bacteria resulted in the enhancement of genome transfer...
  14. pmc Cell-free cloning using phi29 DNA polymerase
    Clyde A Hutchison
    Synthetic Biology Group, The J Craig Venter Institute, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 102:17332-6. 2005
    ..These include environmental DNA samples that have proven difficult to clone and synthetic genes encoding toxic products. The method may also speed genome sequencing by eliminating the need for biological cloning...
  15. doi request reprint Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome
    Daniel G Gibson
    J Craig Venter Institute, Rockville, MD 20850, USA
    Science 319:1215-20. 2008
    ..The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments...
  16. pmc Essential genes of a minimal bacterium
    John I Glass
    Synthetic Biology Group, J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 103:425-30. 2006
    ..Genes encoding proteins of unknown function constitute 28% of the essential protein-coding genes set. Disruption of some genes accelerated M. genitalium growth...
  17. pmc Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotides
    Hamilton O Smith
    Institute for Biological Energy Alternatives, 1901 Research Boulevard, Suite 600, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 100:15440-5. 2003
    ..One such isolate had exactly the intended sequence. We propose to assemble larger genomes by joining separately assembled 5- to 6-kb segments; approximately 60 such segments would be required for a minimal cellular genome...
  18. pmc Similarity-based gene detection: using COGs to find evolutionarily-conserved ORFs
    Bradford C Powell
    Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina, USA
    BMC Bioinformatics 7:31. 2006
    ..Although COG analysis was initially developed to group annotated genes, we applied it to the task of grouping anonymous DNA sequences that may encode proteins...