Daniel G Gibson

Summary

Affiliation: J. Craig Venter Institute
Country: USA

Publications

  1. pmc One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 105:20404-9. 2008
  2. doi request reprint Oligonucleotide assembly in yeast to produce synthetic DNA fragments
    Daniel G Gibson
    Department of Synthetic Biology, J Craig Venter Institute, Inc, Rockville, MD, USA
    Methods Mol Biol 852:11-21. 2012
  3. doi request reprint Enzymatic assembly of DNA molecules up to several hundred kilobases
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, Rockville, Maryland, USA
    Nat Methods 6:343-5. 2009
  4. pmc Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 37:6984-90. 2009
  5. doi request reprint Chemical synthesis of the mouse mitochondrial genome
    Daniel G Gibson
    The J Craig Venter Institute, Rockville, Maryland, USA
    Nat Methods 7:901-3. 2010
  6. doi request reprint Creation of a bacterial cell controlled by a chemically synthesized genome
    Daniel G Gibson
    The J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Science 329:52-6. 2010
  7. pmc Cloning whole bacterial genomes in yeast
    Gwynedd A Benders
    Synthetic Biology and Bioenergy Group, The J Craig Venter Institute, San Diego, CA 92121, USA
    Nucleic Acids Res 38:2558-69. 2010
  8. doi request reprint Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome
    Daniel G Gibson
    J Craig Venter Institute, Rockville, MD 20850, USA
    Science 319:1215-20. 2008
  9. doi request reprint Creating bacterial strains from genomes that have been cloned and engineered in yeast
    Carole Lartigue
    J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Science 325:1693-6. 2009
  10. doi request reprint Transferring whole genomes from bacteria to yeast spheroplasts using entire bacterial cells to reduce DNA shearing
    Bogumil J Karas
    Department of Synthetic Biology and Bioenergy, J Craig Venter Institute, La Jolla, California, USA
    Nat Protoc 9:743-50. 2014

Collaborators

Detail Information

Publications17

  1. pmc One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 105:20404-9. 2008
    ..The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments...
  2. doi request reprint Oligonucleotide assembly in yeast to produce synthetic DNA fragments
    Daniel G Gibson
    Department of Synthetic Biology, J Craig Venter Institute, Inc, Rockville, MD, USA
    Methods Mol Biol 852:11-21. 2012
    ..This straightforward scheme for assembling chemically synthesized oligonucleotides can be a useful tool for building synthetic DNA molecules...
  3. doi request reprint Enzymatic assembly of DNA molecules up to several hundred kilobases
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, Rockville, Maryland, USA
    Nat Methods 6:343-5. 2009
    ..This assembly method can be used to seamlessly construct synthetic and natural genes, genetic pathways and entire genomes, and could be a useful molecular engineering tool...
  4. pmc Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 37:6984-90. 2009
    ..This straightforward scheme for assembling chemically-synthesized oligonucleotides could be a useful tool for building synthetic DNA molecules...
  5. doi request reprint Chemical synthesis of the mouse mitochondrial genome
    Daniel G Gibson
    The J Craig Venter Institute, Rockville, Maryland, USA
    Nat Methods 7:901-3. 2010
    ..As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers...
  6. doi request reprint Creation of a bacterial cell controlled by a chemically synthesized genome
    Daniel G Gibson
    The J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Science 329:52-6. 2010
    ..The new cells have expected phenotypic properties and are capable of continuous self-replication...
  7. pmc Cloning whole bacterial genomes in yeast
    Gwynedd A Benders
    Synthetic Biology and Bioenergy Group, The J Craig Venter Institute, San Diego, CA 92121, USA
    Nucleic Acids Res 38:2558-69. 2010
    ..6 Mb), M. pneumoniae (0.8 Mb) and M. mycoides subspecies capri (1.1 Mb) as yeast circular centromeric plasmids. These genomes appear to be stably maintained in a host that has efficient, well-established methods for DNA manipulation...
  8. doi request reprint Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome
    Daniel G Gibson
    J Craig Venter Institute, Rockville, MD 20850, USA
    Science 319:1215-20. 2008
    ..The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments...
  9. doi request reprint Creating bacterial strains from genomes that have been cloned and engineered in yeast
    Carole Lartigue
    J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Science 325:1693-6. 2009
    ..mycoides. These methods allow the construction of strains that could not be produced with genetic tools available for this bacterium...
  10. doi request reprint Transferring whole genomes from bacteria to yeast spheroplasts using entire bacterial cells to reduce DNA shearing
    Bogumil J Karas
    Department of Synthetic Biology and Bioenergy, J Craig Venter Institute, La Jolla, California, USA
    Nat Protoc 9:743-50. 2014
    ..Excluding the time required for preparing starting cultures and for incubating cells to form final colonies, the protocol can be completed in 3 h. ..
  11. doi request reprint Assembly of large, high G+C bacterial DNA fragments in yeast
    Vladimir N Noskov
    Department of Synthetic Biology and Bioenergy, J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    ACS Synth Biol 1:267-73. 2012
    ..Thus, cloning strategies utilizing yeast recombination with large, high G+C prokaryotic sequences should include yeast origins of replication as a part of the design process...
  12. doi request reprint Gene and genome construction in yeast
    Daniel G Gibson
    The J Craig Venter Institute, San Diego, California, USA
    Curr Protoc Mol Biol . 2011
    ..This unit presents protocols for designing the DNA molecules to be assembled, transforming them into yeast, and confirming their assembly...
  13. doi request reprint Enzymatic assembly of overlapping DNA fragments
    Daniel G Gibson
    J Craig Venter Institute Inc, Synthetic Biology Group, La Jolla, California, USA
    Methods Enzymol 498:349-61. 2011
    ..These assembly methods can be used to seamlessly construct synthetic and natural genes, genetic pathways, and entire genomes and could be very useful for molecular engineering tools...
  14. pmc Sequence analysis of a complete 1.66 Mb Prochlorococcus marinus MED4 genome cloned in yeast
    Christian Tagwerker
    Department of Synthetic Biology and Bioenergy, J Craig Venter Institute, 10355 Science Center Drive, San Diego, CA 92121, USA
    Nucleic Acids Res 40:10375-83. 2012
    ..We thus provide evidence of transformation, replication and maintenance of this 1.66 Mb intact bacterial genome in S. cerevisiae...
  15. doi request reprint Isolation of circular yeast artificial chromosomes for synthetic biology and functional genomics studies
    Vladimir N Noskov
    The J Craig Venter Institute, Rockville, Maryland, USA
    Nat Protoc 6:89-96. 2011
    ..This method for isolating high-quality YAC DNA in microgram quantities should be valuable for functional and synthetic genomic studies. The entire protocol takes ∼3 d to complete...
  16. doi request reprint Direct transfer of whole genomes from bacteria to yeast
    Bogumil J Karas
    Department of Synthetic Biology and Bioenergy, J Craig Venter Institute, San Diego, California, USA
    Nat Methods 10:410-2. 2013
    ..8 megabases (Mb) into yeast under conditions that promote cell fusion. Moreover, we discovered that removal of restriction endonucleases from donor bacteria resulted in the enhancement of genome transfer...
  17. ncbi request reprint Methods and applications for assembling large DNA constructs
    Chuck Merryman
    J Craig Venter Institute, Rockville, MD 20850, USA
    Metab Eng 14:196-204. 2012
    ..We tested the potential of DNA assembly to install rational changes in complex biosynthetic pathways, their potential for generating complex libraries, and consider how various strategies are applicable to metabolic engineering...