Daniel G Gibson

Summary

Affiliation: J. Craig Venter Institute
Country: USA

Publications

  1. ncbi One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 105:20404-9. 2008
  2. ncbi Oligonucleotide assembly in yeast to produce synthetic DNA fragments
    Daniel G Gibson
    Department of Synthetic Biology, J Craig Venter Institute, Inc, Rockville, MD, USA
    Methods Mol Biol 852:11-21. 2012
  3. ncbi Chemical synthesis of the mouse mitochondrial genome
    Daniel G Gibson
    The J Craig Venter Institute, Rockville, Maryland, USA
    Nat Methods 7:901-3. 2010
  4. ncbi Enzymatic assembly of DNA molecules up to several hundred kilobases
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, Rockville, Maryland, USA
    Nat Methods 6:343-5. 2009
  5. ncbi Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 37:6984-90. 2009
  6. ncbi Creation of a bacterial cell controlled by a chemically synthesized genome
    Daniel G Gibson
    The J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Science 329:52-6. 2010
  7. ncbi Cloning whole bacterial genomes in yeast
    Gwynedd A Benders
    Synthetic Biology and Bioenergy Group, The J Craig Venter Institute, San Diego, CA 92121, USA
    Nucleic Acids Res 38:2558-69. 2010
  8. ncbi Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome
    Daniel G Gibson
    J Craig Venter Institute, Rockville, MD 20850, USA
    Science 319:1215-20. 2008
  9. ncbi Creating bacterial strains from genomes that have been cloned and engineered in yeast
    Carole Lartigue
    J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Science 325:1693-6. 2009
  10. ncbi Gene and genome construction in yeast
    Daniel G Gibson
    The J Craig Venter Institute, San Diego, California, USA
    Curr Protoc Mol Biol . 2011

Collaborators

Detail Information

Publications14

  1. ncbi One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 105:20404-9. 2008
    ..The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments...
  2. ncbi Oligonucleotide assembly in yeast to produce synthetic DNA fragments
    Daniel G Gibson
    Department of Synthetic Biology, J Craig Venter Institute, Inc, Rockville, MD, USA
    Methods Mol Biol 852:11-21. 2012
    ..This straightforward scheme for assembling chemically synthesized oligonucleotides can be a useful tool for building synthetic DNA molecules...
  3. ncbi Chemical synthesis of the mouse mitochondrial genome
    Daniel G Gibson
    The J Craig Venter Institute, Rockville, Maryland, USA
    Nat Methods 7:901-3. 2010
    ..As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers...
  4. ncbi Enzymatic assembly of DNA molecules up to several hundred kilobases
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, Rockville, Maryland, USA
    Nat Methods 6:343-5. 2009
    ..This assembly method can be used to seamlessly construct synthetic and natural genes, genetic pathways and entire genomes, and could be a useful molecular engineering tool...
  5. ncbi Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 37:6984-90. 2009
    ..This straightforward scheme for assembling chemically-synthesized oligonucleotides could be a useful tool for building synthetic DNA molecules...
  6. ncbi Creation of a bacterial cell controlled by a chemically synthesized genome
    Daniel G Gibson
    The J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Science 329:52-6. 2010
    ..The new cells have expected phenotypic properties and are capable of continuous self-replication...
  7. ncbi Cloning whole bacterial genomes in yeast
    Gwynedd A Benders
    Synthetic Biology and Bioenergy Group, The J Craig Venter Institute, San Diego, CA 92121, USA
    Nucleic Acids Res 38:2558-69. 2010
    ..6 Mb), M. pneumoniae (0.8 Mb) and M. mycoides subspecies capri (1.1 Mb) as yeast circular centromeric plasmids. These genomes appear to be stably maintained in a host that has efficient, well-established methods for DNA manipulation...
  8. ncbi Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome
    Daniel G Gibson
    J Craig Venter Institute, Rockville, MD 20850, USA
    Science 319:1215-20. 2008
    ..The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments...
  9. ncbi Creating bacterial strains from genomes that have been cloned and engineered in yeast
    Carole Lartigue
    J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Science 325:1693-6. 2009
    ..mycoides. These methods allow the construction of strains that could not be produced with genetic tools available for this bacterium...
  10. ncbi Gene and genome construction in yeast
    Daniel G Gibson
    The J Craig Venter Institute, San Diego, California, USA
    Curr Protoc Mol Biol . 2011
    ..This unit presents protocols for designing the DNA molecules to be assembled, transforming them into yeast, and confirming their assembly...
  11. ncbi Enzymatic assembly of overlapping DNA fragments
    Daniel G Gibson
    J Craig Venter Institute Inc, Synthetic Biology Group, La Jolla, California, USA
    Methods Enzymol 498:349-61. 2011
    ..These assembly methods can be used to seamlessly construct synthetic and natural genes, genetic pathways, and entire genomes and could be very useful for molecular engineering tools...
  12. ncbi Sequence analysis of a complete 1.66 Mb Prochlorococcus marinus MED4 genome cloned in yeast
    Christian Tagwerker
    Department of Synthetic Biology and Bioenergy, J Craig Venter Institute, 10355 Science Center Drive, San Diego, CA 92121, USA
    Nucleic Acids Res 40:10375-83. 2012
    ..We thus provide evidence of transformation, replication and maintenance of this 1.66 Mb intact bacterial genome in S. cerevisiae...
  13. ncbi Isolation of circular yeast artificial chromosomes for synthetic biology and functional genomics studies
    Vladimir N Noskov
    The J Craig Venter Institute, Rockville, Maryland, USA
    Nat Protoc 6:89-96. 2011
    ..This method for isolating high-quality YAC DNA in microgram quantities should be valuable for functional and synthetic genomic studies. The entire protocol takes ∼3 d to complete...
  14. ncbi Methods and applications for assembling large DNA constructs
    Chuck Merryman
    J Craig Venter Institute, Rockville, MD 20850, USA
    Metab Eng 14:196-204. 2012
    ..We tested the potential of DNA assembly to install rational changes in complex biosynthetic pathways, their potential for generating complex libraries, and consider how various strategies are applicable to metabolic engineering...