Walt F Lima

Summary

Affiliation: Isis Pharmaceuticals
Country: USA

Publications

  1. Lima W, Murray H, Nichols J, Wu H, Sun H, Prakash T, et al. Human Dicer binds short single-strand and double-strand RNA with high affinity and interacts with different regions of the nucleic acids. J Biol Chem. 2009;284:2535-48 pubmed publisher
    ..These data suggest that the siRNAs and ssRNAs interact predominantly with the PAZ domain of the enzyme. Finally, the tightest binding siRNAs were also more potent inhibitors of gene expression. ..
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    Lima W, Crooke S. Binding affinity and specificity of Escherichia coli RNase H1: impact on the kinetics of catalysis of antisense oligonucleotide-RNA hybrids. Biochemistry. 1997;36:390-8 pubmed
    ..These results offer further insights into the physical binding properties of the RNase H-substrate interaction as well as the design of effective antisense oligonucleotides. ..
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    Lima W, Wu H, Nichols J, Manalili S, Drader J, Hofstadler S, et al. Human RNase H1 activity is regulated by a unique redox switch formed between adjacent cysteines. J Biol Chem. 2003;278:14906-12 pubmed
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    Lima W, Wu H, Nichols J, Prakash T, Ravikumar V, Crooke S. Human RNase H1 uses one tryptophan and two lysines to position the enzyme at the 3'-DNA/5'-RNA terminus of the heteroduplex substrate. J Biol Chem. 2003;278:49860-7 pubmed
    ..These results offer further insights into the interaction between human RNase H1 and the heteroduplex substrate as well as approaches to enhance the design of effective antisense oligonucleotides. ..
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    Lima W, Nichols J, Wu H, Prakash T, Migawa M, Wyrzykiewicz T, et al. Structural requirements at the catalytic site of the heteroduplex substrate for human RNase H1 catalysis. J Biol Chem. 2004;279:36317-26 pubmed
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    Lima W, Rose J, Nichols J, Wu H, Migawa M, Wyrzykiewicz T, et al. The positional influence of the helical geometry of the heteroduplex substrate on human RNase H1 catalysis. Mol Pharmacol. 2007;71:73-82 pubmed
    ..Finally, these results provide a method for enhancing the human RNase H1 activity of chimeric antisense oligonucleotides (ASO) as well as the design of more potent ASO drugs. ..
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    Lima W, Rose J, Nichols J, Wu H, Migawa M, Wyrzykiewicz T, et al. Human RNase H1 discriminates between subtle variations in the structure of the heteroduplex substrate. Mol Pharmacol. 2007;71:83-91 pubmed
    ..The data presented here offer further insights into the role substrate structure plays in directing human RNase H1 activity as well as the design of effective ASOs. ..
  8. Lima W, Wu H, Nichols J, Sun H, Murray H, Crooke S. Binding and cleavage specificities of human Argonaute2. J Biol Chem. 2009;284:26017-28 pubmed publisher