Ravindra Singh

Summary

Affiliation: Iowa State University
Country: USA

Publications

  1. pmc In vivo selection reveals combinatorial controls that define a critical exon in the spinal muscular atrophy genes
    Natalia N Singh
    Department of Medicine LRB 326, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605 2324, USA
    RNA 10:1291-305. 2004
  2. pmc TIA1 prevents skipping of a critical exon associated with spinal muscular atrophy
    Natalia N Singh
    Department of Biomedical Sciences, College of Veterinary Medicine, 2034 Veterinary Medicine Bldg, Iowa State University, Ames, IA 50011, USA
    Mol Cell Biol 31:935-54. 2011
  3. ncbi Evolving concepts on human SMN pre-mRNA splicing
    Ravindra N Singh
    Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA
    RNA Biol 4:7-10. 2007
  4. pmc An antisense microwalk reveals critical role of an intronic position linked to a unique long-distance interaction in pre-mRNA splicing
    Natalia N Singh
    Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA
    RNA 16:1167-81. 2010
  5. pmc A short antisense oligonucleotide masking a unique intronic motif prevents skipping of a critical exon in spinal muscular atrophy
    Natalia N Singh
    Department of Biomedical Sciences, Iowa State University, Ames, IA 01605, USA
    RNA Biol 6:341-50. 2009
  6. ncbi Unfolding the mystery of alternative splicing through a unique method of in vivo selection
    Ravindra N Singh
    Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605 2324, USA
    Front Biosci 12:3263-72. 2007
  7. pmc Modulating role of RNA structure in alternative splicing of a critical exon in the spinal muscular atrophy genes
    Natalia N Singh
    Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605 2324, USA
    Nucleic Acids Res 35:371-89. 2007
  8. pmc Splicing of a critical exon of human Survival Motor Neuron is regulated by a unique silencer element located in the last intron
    Nirmal K Singh
    Department of Medicine LRB 326, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Mol Cell Biol 26:1333-46. 2006
  9. ncbi The regulation and regulatory activities of alternative splicing of the SMN gene
    Natalia N Singh
    Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605 2324, USA
    Crit Rev Eukaryot Gene Expr 14:271-85. 2004
  10. ncbi An extended inhibitory context causes skipping of exon 7 of SMN2 in spinal muscular atrophy
    Natalia N Singh
    Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605 2324, USA
    Biochem Biophys Res Commun 315:381-8. 2004

Collaborators

  • Alan Lambowitz
  • Natalia N Singh
  • Elliot J Androphy
  • Dhruva Bhattacharya
  • Maria Shishimorova
  • Nirmal K Singh
  • Eric W Ottesen
  • Joonbae Seo
  • Katrin Hollinger
  • Laxman Gangwani
  • Lu Cheng Cao

Detail Information

Publications11

  1. pmc In vivo selection reveals combinatorial controls that define a critical exon in the spinal muscular atrophy genes
    Natalia N Singh
    Department of Medicine LRB 326, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605 2324, USA
    RNA 10:1291-305. 2004
    ..This method offers unparallel advantage for determining the relative strength of splice sites, as well as for identifying the novel exonic cis-elements...
  2. pmc TIA1 prevents skipping of a critical exon associated with spinal muscular atrophy
    Natalia N Singh
    Department of Biomedical Sciences, College of Veterinary Medicine, 2034 Veterinary Medicine Bldg, Iowa State University, Ames, IA 50011, USA
    Mol Cell Biol 31:935-54. 2011
    ..Our findings expand the scope of TIA1/TIAR in genome-wide regulation of alternative splicing under normal and pathological conditions...
  3. ncbi Evolving concepts on human SMN pre-mRNA splicing
    Ravindra N Singh
    Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA
    RNA Biol 4:7-10. 2007
    ..Considering an antisense-based strategy confers a unique advantage of sequence specificity, availability of many target worthy cis-elements holds strong potential for antisense-mediated therapy of SMA...
  4. pmc An antisense microwalk reveals critical role of an intronic position linked to a unique long-distance interaction in pre-mRNA splicing
    Natalia N Singh
    Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA
    RNA 16:1167-81. 2010
    ....
  5. pmc A short antisense oligonucleotide masking a unique intronic motif prevents skipping of a critical exon in spinal muscular atrophy
    Natalia N Singh
    Department of Biomedical Sciences, Iowa State University, Ames, IA 01605, USA
    RNA Biol 6:341-50. 2009
    ..Our findings underscore the advantages and unmatched potential of very short ASOs in splicing modulation in vivo...
  6. ncbi Unfolding the mystery of alternative splicing through a unique method of in vivo selection
    Ravindra N Singh
    Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605 2324, USA
    Front Biosci 12:3263-72. 2007
    ..Considering this unbiased method uses in vivo conditions, it has potential to identify critical regulatory elements as therapeutic targets for a growing number of splicing-associated diseases...
  7. pmc Modulating role of RNA structure in alternative splicing of a critical exon in the spinal muscular atrophy genes
    Natalia N Singh
    Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605 2324, USA
    Nucleic Acids Res 35:371-89. 2007
    ..Our findings reveal for the first time the critical role of RNA structure in regulation of alternative splicing of human SMN...
  8. pmc Splicing of a critical exon of human Survival Motor Neuron is regulated by a unique silencer element located in the last intron
    Nirmal K Singh
    Department of Medicine LRB 326, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Mol Cell Biol 26:1333-46. 2006
    ....
  9. ncbi The regulation and regulatory activities of alternative splicing of the SMN gene
    Natalia N Singh
    Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605 2324, USA
    Crit Rev Eukaryot Gene Expr 14:271-85. 2004
    ..Recent advances in our understanding of SMN gene splicing reveals multiple challenges that are specific to in vivo regulation, which we now know is intimately connected with other biological pathways...
  10. ncbi An extended inhibitory context causes skipping of exon 7 of SMN2 in spinal muscular atrophy
    Natalia N Singh
    Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605 2324, USA
    Biochem Biophys Res Commun 315:381-8. 2004
    ....
  11. ncbi Binding of a group II intron-encoded reverse transcriptase/maturase to its high affinity intron RNA binding site involves sequence-specific recognition and autoregulates translation
    Ravindra N Singh
    Institute for Cellular and Molecular Biology, Department of Chemistry and Biochemistry, and Section of Molecular Genetics and Microbiology, School of Biological Sciences, University of Texas at Austin, 78712, USA
    J Mol Biol 318:287-303. 2002
    ..The recognition of the DIVa loop-stem-loop structure accounts, in part, for the intron specificity of group II intron maturases and has parallels in template-recognition mechanisms used by other reverse transcriptases...

Research Grants8

  1. Targeting a novel silencer to correct SMN2 splicing in Spinal Muscular Atrophy
    Ravindra Singh; Fiscal Year: 2007
    ..To explore the therapeutic potential of antisense oligos, we will extend our study to the mice models of SMA. ..
  2. Characterization of a complex regulatory element of Spinal Muscular Atrophy genes
    Ravindra Singh; Fiscal Year: 2007
    ..Here, we will characterize a novel intronic element that plays a critical role in pathogenesis of SMA. In addition, we will use this element as a target for the antisense-mediated correction of aberrant splicing in SMA. ..
  3. Targeting a novel silencer to correct SMN2 splicing in Spinal Muscular Atrophy
    Ravindra Singh; Fiscal Year: 2007
    ..To explore the therapeutic potential of antisense oligos, we will extend our study to the mice models of SMA. ..
  4. Characterization of a complex regulatory element of Spinal Muscular Atrophy genes
    Ravindra Singh; Fiscal Year: 2009
    ..Here, we will characterize a novel intronic element that plays a critical role in pathogenesis of SMA. In addition, we will use this element as a target for the antisense-mediated correction of aberrant splicing in SMA. ..
  5. Characterization of a complex regulatory element of Spinal Muscular Atrophy genes
    Ravindra N Singh; Fiscal Year: 2010
    ..Here, we will characterize a novel intronic element that plays a critical role in pathogenesis of SMA. In addition, we will use this element as a target for the antisense-mediated correction of aberrant splicing in SMA. ..
  6. Characterization of a complex regulatory element of Spinal Muscular Atrophy genes
    Ravindra Singh; Fiscal Year: 2009
    ..Here, we will characterize a novel intronic element that plays a critical role in pathogenesis of SMA. In addition, we will use this element as a target for the antisense-mediated correction of aberrant splicing in SMA. ..