REUBEN JOHN PETERSSummaryAffiliation: Iowa State University Country: USA Publications
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Publications
Rice contains two disparate ent-copalyl diphosphate synthases with distinct metabolic functionsSladjana Prisic
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
Plant Physiol 136:4228-36. 2004....
Uncovering the complex metabolic network underlying diterpenoid phytoalexin biosynthesis in rice and other cereal crop plantsReuben J Peters
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
Phytochemistry 67:2307-17. 2006....- Terpenoid synthase structures: a so far incomplete view of complex catalysisYang Gao
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
Nat Prod Rep 29:1153-75. 2012..Accordingly, selected examples are discussed here to demonstrate our current understanding, its limits, and potential ways forward... - Increasing diterpene yield with a modular metabolic engineering system in E. coli: comparison of MEV and MEP isoprenoid precursor pathway engineeringDana Morrone
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
Appl Microbiol Biotechnol 85:1893-906. 2010..Finally, we reiterate here that this modular approach is expandable and should be easily adaptable to the production of any terpenoid natural product... - Functional identification of rice syn-copalyl diphosphate synthase and its role in initiating biosynthesis of diterpenoid phytoalexin/allelopathic natural productsMeimei Xu
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
Plant J 39:309-18. 2004..One particularly notable feature is the specific conservation of the functionally cryptic 'insertional' sequence element in class II terpene synthases, indicating that this region is important for the corresponding cyclization reaction... - Probing the role of the DXDD motif in Class II diterpene cyclasesSladjana Prisic
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Molecular Biology Building, Ames, IA 50011, USA
Chembiochem 8:869-74. 2007 
Electrostatic effects on (di)terpene synthase product outcomeKe Zhou
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
Chem Commun (Camb) 47:4074-80. 2011..Thus, (di)terpene synthases seem to mediate specific reaction outcomes, at least in part, by providing electrostatic effects to counteract those exerted by the pyrophosphate co-product...- CYP76M7 is an ent-cassadiene C11alpha-hydroxylase defining a second multifunctional diterpenoid biosynthetic gene cluster in riceSivakumar Swaminathan
Department of Biochemistry, Iowa State University, Ames, Iowa 50011, USA
Plant Cell 21:3315-25. 2009.... - Edaxadiene: a new bioactive diterpene from Mycobacterium tuberculosisFrancis M Mann
Iowa State University, Department of Biochemistry, Biophysics, and Molecular Biology, Ames, Iowa 5001, USA
J Am Chem Soc 131:17526-7. 2009.... - A single residue switch for Mg(2+)-dependent inhibition characterizes plant class II diterpene cyclases from primary and secondary metabolismFrancis M Mann
From the Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
J Biol Chem 285:20558-63. 2010.... - Characterization and inhibition of a class II diterpene cyclase from Mycobacterium tuberculosis: implications for tuberculosisFrancis M Mann
Department of Biochemistry, Iowa State University, Ames, Iowa 50011, USA
J Biol Chem 284:23574-9. 2009..Thus, we hypothesize that the inability to produce edaxadiene may be a contributing factor in the decreased infectivity and/or virulence of M. bovis relative to M. tuberculosis in humans... - Investigating the conservation pattern of a putative second terpene synthase divalent metal binding motif in plantsKe Zhou
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Molecular Biology Building, Rm 4216, Ames, IA 50011, United States
Phytochemistry 70:366-9. 2009..In any case, our results are consistent with the observed conservation pattern for this putative second divalent metal ion binding motif in plant TPS... - Identification of syn-pimara-7,15-diene synthase reveals functional clustering of terpene synthases involved in rice phytoalexin/allelochemical biosynthesisP Ross Wilderman
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
Plant Physiol 135:2098-105. 2004.... - Synergistic substrate inhibition of ent-copalyl diphosphate synthase: a potential feed-forward inhibition mechanism limiting gibberellin metabolismSladjana Prisic
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
Plant Physiol 144:445-54. 2007..Finally, the observed synergistic substrate/feed-forward inhibition of CPS also seems to provide a novel example of direct regulation of enzymatic activity in hormone biosynthesis... - An unexpected diterpene cyclase from rice: functional identification of a stemodene synthaseDana Morrone
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
Arch Biochem Biophys 448:133-40. 2006.... - A modular approach for facile biosynthesis of labdane-related diterpenesAnthony Cyr
Iowa State University, Department of Biochemistry, Biophysics, and Molecular Biology, Ames, Iowa 50011, USA
J Am Chem Soc 129:6684-5. 2007 - Increasing complexity of a diterpene synthase reaction with a single residue switchDana Morrone
Iowa State University, Department of Biochemistry, Biophysics, and Molecular Biology, Ames, Iowa 50011, USA
J Am Chem Soc 130:5400-1. 2008..Furthermore, the profound plasticity of diterpene synthases exemplified by this single residue switch for product outcome is consistent with the screening/diversity-oriented hypothesis of natural products metabolism... - A single residue switch converts abietadiene synthase into a pimaradiene specific cyclaseP Ross Wilderman
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
J Am Chem Soc 129:15736-7. 2007..Furthermore, these defined single residue switches may be useful in engineering product outcome in diterpene synthases more generally... - Following evolution's lead to a single residue switch for diterpene synthase product outcomeMeimei Xu
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
Proc Natl Acad Sci U S A 104:7397-401. 2007.... - Abietadiene synthase catalysis: conserved residues involved in protonation-initiated cyclization of geranylgeranyl diphosphate to (+)-copalyl diphosphateReuben J Peters
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USA
Biochemistry 41:1836-42. 2002..A functional role was also suggested for the cryptic insertional element found in abietadiene synthase and other diterpene synthases that carry out similar protonation-initiated cyclizations... - Monoterpene biosynthesis pathway construction in Escherichia coliOra A Carter
Institute of Biological Chemistry, and Plant Physiology Program, Washington State University, Pullman, WA 99164-6340, USA
Phytochemistry 64:425-33. 2003.... - 16-Aza-ent-beyerane and 16-Aza-ent-trachylobane: potent mechanism-based inhibitors of recombinant ent-kaurene synthase from Arabidopsis thalianaArnab Roy
Department of Chemistry University of Illinois, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
J Am Chem Soc 129:12453-60. 2007.... - Bifunctional abietadiene synthase: mutual structural dependence of the active sites for protonation-initiated and ionization-initiated cyclizationsReuben J Peters
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USA
Biochemistry 42:2700-7. 2003..Thus, the two cyclization sites of abietadiene synthase cannot be dissected into catalytically distinct domains, and, therefore, abietadiene synthase is unlikely to have arisen by fusion of two previously independent genes...
Research Grants
- A Modular Approach for Combinatorial Biosynthesis of Functionalized TerpenoidsREUBEN JOHN PETERS; Fiscal Year: 2010..Bacterial production will provide an effective source for production of the larger amounts of these natural products necessary for detailed investigation of their promising biological activity. ..
- A Modular Approach for Combinatorial Biosynthesis of Functionalized TerpenoidsREUBEN PETERS; Fiscal Year: 2009..Bacterial production will provide an effective source for production of the larger amounts of these natural products necessary for detailed investigation of their promising biological activity. ..
- Enzymatic Cyclization to Labdanes and Related Diterpenoid Natural ProductsREUBEN PETERS; Fiscal Year: 2007..abstract_text> ..
- Enzymatic Cyclization to Labdanes and Related Diterpenoid Natural ProductsREUBEN PETERS; Fiscal Year: 2007....
- Enzymatic Cyclization to Labdanes and Related Diterpenoid Natural ProductsREUBEN JOHN PETERS; Fiscal Year: 2010....