REUBEN JOHN PETERS

Summary

Affiliation: Iowa State University
Country: USA

Publications

  1. pmc Rice contains two disparate ent-copalyl diphosphate synthases with distinct metabolic functions
    Sladjana Prisic
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Plant Physiol 136:4228-36. 2004
  2. ncbi request reprint Uncovering the complex metabolic network underlying diterpenoid phytoalexin biosynthesis in rice and other cereal crop plants
    Reuben J Peters
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Phytochemistry 67:2307-17. 2006
  3. pmc Terpenoid synthase structures: a so far incomplete view of complex catalysis
    Yang Gao
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Nat Prod Rep 29:1153-75. 2012
  4. pmc Increasing diterpene yield with a modular metabolic engineering system in E. coli: comparison of MEV and MEP isoprenoid precursor pathway engineering
    Dana Morrone
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Appl Microbiol Biotechnol 85:1893-906. 2010
  5. ncbi request reprint Functional identification of rice syn-copalyl diphosphate synthase and its role in initiating biosynthesis of diterpenoid phytoalexin/allelopathic natural products
    Meimei Xu
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Plant J 39:309-18. 2004
  6. ncbi request reprint Probing the role of the DXDD motif in Class II diterpene cyclases
    Sladjana Prisic
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Molecular Biology Building, Ames, IA 50011, USA
    Chembiochem 8:869-74. 2007
  7. pmc Electrostatic effects on (di)terpene synthase product outcome
    Ke Zhou
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Chem Commun (Camb) 47:4074-80. 2011
  8. pmc Investigating the conservation pattern of a putative second terpene synthase divalent metal binding motif in plants
    Ke Zhou
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Molecular Biology Building, Rm 4216, Ames, IA 50011, United States
    Phytochemistry 70:366-9. 2009
  9. pmc Characterization and inhibition of a class II diterpene cyclase from Mycobacterium tuberculosis: implications for tuberculosis
    Francis M Mann
    Department of Biochemistry, Iowa State University, Ames, Iowa 50011, USA
    J Biol Chem 284:23574-9. 2009
  10. pmc Edaxadiene: a new bioactive diterpene from Mycobacterium tuberculosis
    Francis M Mann
    Iowa State University, Department of Biochemistry, Biophysics, and Molecular Biology, Ames, Iowa 5001, USA
    J Am Chem Soc 131:17526-7. 2009

Collaborators

  • Meimei Xu
  • Sladjana Prisic
  • Dana Morrone
  • P Ross Wilderman
  • Robert M Coates
  • Francis M Mann
  • Ke Zhou
  • Mara K Determan
  • D Bruce Fulton
  • Yang Gao
  • Sivakumar Swaminathan
  • Arnab Roy
  • Anthony Cyr
  • Yinghua Jin
  • Ora A Carter
  • Richard B Honzatko
  • Emily K Davenport
  • Luke Lowry
  • David M Hershey
  • Huayou Hu
  • Xiaoming Chen
  • David G Russell
  • Qiang Wang
  • Mara Determan
  • Jianjun Xu
  • Frank G Roberts
  • Suh Yeon Choi
  • Matthew L Hillwig
  • Rodney Croteau

Detail Information

Publications23

  1. pmc Rice contains two disparate ent-copalyl diphosphate synthases with distinct metabolic functions
    Sladjana Prisic
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Plant Physiol 136:4228-36. 2004
    ....
  2. ncbi request reprint Uncovering the complex metabolic network underlying diterpenoid phytoalexin biosynthesis in rice and other cereal crop plants
    Reuben J Peters
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Phytochemistry 67:2307-17. 2006
    ....
  3. pmc Terpenoid synthase structures: a so far incomplete view of complex catalysis
    Yang Gao
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Nat Prod Rep 29:1153-75. 2012
    ..Accordingly, selected examples are discussed here to demonstrate our current understanding, its limits, and potential ways forward...
  4. pmc Increasing diterpene yield with a modular metabolic engineering system in E. coli: comparison of MEV and MEP isoprenoid precursor pathway engineering
    Dana Morrone
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Appl Microbiol Biotechnol 85:1893-906. 2010
    ..Finally, we reiterate here that this modular approach is expandable and should be easily adaptable to the production of any terpenoid natural product...
  5. ncbi request reprint Functional identification of rice syn-copalyl diphosphate synthase and its role in initiating biosynthesis of diterpenoid phytoalexin/allelopathic natural products
    Meimei Xu
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Plant J 39:309-18. 2004
    ..One particularly notable feature is the specific conservation of the functionally cryptic 'insertional' sequence element in class II terpene synthases, indicating that this region is important for the corresponding cyclization reaction...
  6. ncbi request reprint Probing the role of the DXDD motif in Class II diterpene cyclases
    Sladjana Prisic
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Molecular Biology Building, Ames, IA 50011, USA
    Chembiochem 8:869-74. 2007
  7. pmc Electrostatic effects on (di)terpene synthase product outcome
    Ke Zhou
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Chem Commun (Camb) 47:4074-80. 2011
    ..Thus, (di)terpene synthases seem to mediate specific reaction outcomes, at least in part, by providing electrostatic effects to counteract those exerted by the pyrophosphate co-product...
  8. pmc Investigating the conservation pattern of a putative second terpene synthase divalent metal binding motif in plants
    Ke Zhou
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Molecular Biology Building, Rm 4216, Ames, IA 50011, United States
    Phytochemistry 70:366-9. 2009
    ..In any case, our results are consistent with the observed conservation pattern for this putative second divalent metal ion binding motif in plant TPS...
  9. pmc Characterization and inhibition of a class II diterpene cyclase from Mycobacterium tuberculosis: implications for tuberculosis
    Francis M Mann
    Department of Biochemistry, Iowa State University, Ames, Iowa 50011, USA
    J Biol Chem 284:23574-9. 2009
    ..Thus, we hypothesize that the inability to produce edaxadiene may be a contributing factor in the decreased infectivity and/or virulence of M. bovis relative to M. tuberculosis in humans...
  10. pmc Edaxadiene: a new bioactive diterpene from Mycobacterium tuberculosis
    Francis M Mann
    Iowa State University, Department of Biochemistry, Biophysics, and Molecular Biology, Ames, Iowa 5001, USA
    J Am Chem Soc 131:17526-7. 2009
    ....
  11. pmc A single residue switch for Mg(2+)-dependent inhibition characterizes plant class II diterpene cyclases from primary and secondary metabolism
    Francis M Mann
    From the Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Biol Chem 285:20558-63. 2010
    ....
  12. pmc CYP76M7 is an ent-cassadiene C11alpha-hydroxylase defining a second multifunctional diterpenoid biosynthetic gene cluster in rice
    Sivakumar Swaminathan
    Department of Biochemistry, Iowa State University, Ames, Iowa 50011, USA
    Plant Cell 21:3315-25. 2009
    ....
  13. doi request reprint Increasing complexity of a diterpene synthase reaction with a single residue switch
    Dana Morrone
    Iowa State University, Department of Biochemistry, Biophysics, and Molecular Biology, Ames, Iowa 50011, USA
    J Am Chem Soc 130:5400-1. 2008
    ..Furthermore, the profound plasticity of diterpene synthases exemplified by this single residue switch for product outcome is consistent with the screening/diversity-oriented hypothesis of natural products metabolism...
  14. pmc A single residue switch converts abietadiene synthase into a pimaradiene specific cyclase
    P Ross Wilderman
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Am Chem Soc 129:15736-7. 2007
    ..Furthermore, these defined single residue switches may be useful in engineering product outcome in diterpene synthases more generally...
  15. pmc A modular approach for facile biosynthesis of labdane-related diterpenes
    Anthony Cyr
    Iowa State University, Department of Biochemistry, Biophysics, and Molecular Biology, Ames, Iowa 50011, USA
    J Am Chem Soc 129:6684-5. 2007
  16. pmc Following evolution's lead to a single residue switch for diterpene synthase product outcome
    Meimei Xu
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Proc Natl Acad Sci U S A 104:7397-401. 2007
    ....
  17. pmc Synergistic substrate inhibition of ent-copalyl diphosphate synthase: a potential feed-forward inhibition mechanism limiting gibberellin metabolism
    Sladjana Prisic
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Plant Physiol 144:445-54. 2007
    ..Finally, the observed synergistic substrate/feed-forward inhibition of CPS also seems to provide a novel example of direct regulation of enzymatic activity in hormone biosynthesis...
  18. ncbi request reprint An unexpected diterpene cyclase from rice: functional identification of a stemodene synthase
    Dana Morrone
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Arch Biochem Biophys 448:133-40. 2006
    ....
  19. pmc Identification of syn-pimara-7,15-diene synthase reveals functional clustering of terpene synthases involved in rice phytoalexin/allelochemical biosynthesis
    P Ross Wilderman
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Plant Physiol 135:2098-105. 2004
    ....
  20. pmc 16-Aza-ent-beyerane and 16-Aza-ent-trachylobane: potent mechanism-based inhibitors of recombinant ent-kaurene synthase from Arabidopsis thaliana
    Arnab Roy
    Department of Chemistry University of Illinois, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
    J Am Chem Soc 129:12453-60. 2007
    ....
  21. ncbi request reprint Monoterpene biosynthesis pathway construction in Escherichia coli
    Ora A Carter
    Institute of Biological Chemistry, and Plant Physiology Program, Washington State University, Pullman, WA 99164 6340, USA
    Phytochemistry 64:425-33. 2003
    ....
  22. ncbi request reprint Bifunctional abietadiene synthase: mutual structural dependence of the active sites for protonation-initiated and ionization-initiated cyclizations
    Reuben J Peters
    Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164 6340, USA
    Biochemistry 42:2700-7. 2003
    ..Thus, the two cyclization sites of abietadiene synthase cannot be dissected into catalytically distinct domains, and, therefore, abietadiene synthase is unlikely to have arisen by fusion of two previously independent genes...
  23. ncbi request reprint Abietadiene synthase catalysis: conserved residues involved in protonation-initiated cyclization of geranylgeranyl diphosphate to (+)-copalyl diphosphate
    Reuben J Peters
    Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164 6340, USA
    Biochemistry 41:1836-42. 2002
    ..A functional role was also suggested for the cryptic insertional element found in abietadiene synthase and other diterpene synthases that carry out similar protonation-initiated cyclizations...

Research Grants10

  1. A Modular Approach for Combinatorial Biosynthesis of Functionalized Terpenoids
    REUBEN JOHN PETERS; Fiscal Year: 2010
    ..Bacterial production will provide an effective source for production of the larger amounts of these natural products necessary for detailed investigation of their promising biological activity. ..
  2. A Modular Approach for Combinatorial Biosynthesis of Functionalized Terpenoids
    REUBEN PETERS; Fiscal Year: 2009
    ..Bacterial production will provide an effective source for production of the larger amounts of these natural products necessary for detailed investigation of their promising biological activity. ..
  3. Enzymatic Cyclization to Labdanes and Related Diterpenoid Natural Products
    REUBEN PETERS; Fiscal Year: 2007
    ..abstract_text> ..
  4. Enzymatic Cyclization to Labdanes and Related Diterpenoid Natural Products
    REUBEN PETERS; Fiscal Year: 2007
    ....
  5. Enzymatic Cyclization to Labdanes and Related Diterpenoid Natural Products
    REUBEN JOHN PETERS; Fiscal Year: 2010
    ....