Tanja Opriessnig

Summary

Affiliation: Iowa State University
Country: USA

Publications

  1. ncbi request reprint Porcine circovirus type 2 associated disease: update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    J Vet Diagn Invest 19:591-615. 2007
  2. doi request reprint Erysipelothrix rhusiopathiae isolates recovered from fish, a harbour seal (Phoca vitulina) and the marine environment are capable of inducing characteristic cutaneous lesions in pigs
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    J Comp Pathol 148:365-72. 2013
  3. doi request reprint Emergence of a novel mutant PCV2b variant associated with clinical PCVAD in two vaccinated pig farms in the U.S. concurrently infected with PPV2
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA
    Vet Microbiol 163:177-83. 2013
  4. doi request reprint Current state of knowledge on porcine circovirus type 2-associated lesions
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA
    Vet Pathol 50:23-38. 2013
  5. doi request reprint Evaluation of diagnostic assays for the serological detection of Actinobacillus pleuropneumoniae on samples of known or unknown exposure
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA
    J Vet Diagn Invest 25:61-71. 2013
  6. doi request reprint A PCV2 vaccine based on genotype 2b is more effective than a 2a-based vaccine to protect against PCV2b or combined PCV2a/2b viremia in pigs with concurrent PCV2, PRRSV and PPV infection
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA
    Vaccine 31:487-94. 2013
  7. doi request reprint Effect of porcine circovirus type 2a or 2b on infection kinetics and pathogenicity of two genetically divergent strains of porcine reproductive and respiratory syndrome virus in the conventional pig model
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Vet Microbiol 158:69-81. 2012
  8. doi request reprint Concurrent infections are important for expression of porcine circovirus associated disease
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50010, USA
    Virus Res 164:20-32. 2012
  9. doi request reprint An experimental live chimeric porcine circovirus 1-2a vaccine decreases porcine circovirus 2b viremia when administered intramuscularly or orally in a porcine circovirus 2b and porcine reproductive and respiratory syndrome virus dual-challenge model
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA
    Microbiol Immunol 55:863-73. 2011
  10. doi request reprint Comparison of the effectiveness of passive (dam) versus active (piglet) immunization against porcine circovirus type 2 (PCV2) and impact of passively derived PCV2 vaccine-induced immunity on vaccination
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Vet Microbiol 142:177-83. 2010

Collaborators

Detail Information

Publications68

  1. ncbi request reprint Porcine circovirus type 2 associated disease: update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    J Vet Diagn Invest 19:591-615. 2007
    ..This article is intended to discuss the most common disease manifestations, pathogenesis, diagnostic approaches, and intervention strategies associated with PCVAD in North America...
  2. doi request reprint Erysipelothrix rhusiopathiae isolates recovered from fish, a harbour seal (Phoca vitulina) and the marine environment are capable of inducing characteristic cutaneous lesions in pigs
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    J Comp Pathol 148:365-72. 2013
    ..infection. The results of this study indicate that the marine E. rhusiopathiae isolates have greater genetic and antigenic diversity than pig isolates and are capable of inducing classical skin lesions in pigs...
  3. doi request reprint Emergence of a novel mutant PCV2b variant associated with clinical PCVAD in two vaccinated pig farms in the U.S. concurrently infected with PPV2
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA
    Vet Microbiol 163:177-83. 2013
    ..In addition, emerging porcine parvovirus type 2 (PPV2) was detected in 55% of the serum samples (73/132), perhaps implying that PPV2 could be a cofactor in cases of PCVAD...
  4. doi request reprint Current state of knowledge on porcine circovirus type 2-associated lesions
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA
    Vet Pathol 50:23-38. 2013
    ..It is now widely accepted that PCV2 can be further subdivided into different types, of which PCV2a and PCV2b are present worldwide and of greatest importance. This review will focus on PCV2-associated lesions in different organ systems...
  5. doi request reprint Evaluation of diagnostic assays for the serological detection of Actinobacillus pleuropneumoniae on samples of known or unknown exposure
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA
    J Vet Diagn Invest 25:61-71. 2013
    ..pleuropneumoniae exposure, differences in assay targets complicate test choices. Decisions on which assay or combination of assays to use depend on the specific reasons for running the assays...
  6. doi request reprint A PCV2 vaccine based on genotype 2b is more effective than a 2a-based vaccine to protect against PCV2b or combined PCV2a/2b viremia in pigs with concurrent PCV2, PRRSV and PPV infection
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA
    Vaccine 31:487-94. 2013
    ....
  7. doi request reprint Effect of porcine circovirus type 2a or 2b on infection kinetics and pathogenicity of two genetically divergent strains of porcine reproductive and respiratory syndrome virus in the conventional pig model
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Vet Microbiol 158:69-81. 2012
    ..This work further emphasizes in vivo replication differences among PRRSV strains and the importance of coinfecting pathogens...
  8. doi request reprint Concurrent infections are important for expression of porcine circovirus associated disease
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50010, USA
    Virus Res 164:20-32. 2012
    ....
  9. doi request reprint An experimental live chimeric porcine circovirus 1-2a vaccine decreases porcine circovirus 2b viremia when administered intramuscularly or orally in a porcine circovirus 2b and porcine reproductive and respiratory syndrome virus dual-challenge model
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA
    Microbiol Immunol 55:863-73. 2011
    ..Under the conditions of this study, the live attenuated PCV1-2a vaccine was safe and provided immune protection resulting in reduction of viremia. The IM route provided the most effective protection...
  10. doi request reprint Comparison of the effectiveness of passive (dam) versus active (piglet) immunization against porcine circovirus type 2 (PCV2) and impact of passively derived PCV2 vaccine-induced immunity on vaccination
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Vet Microbiol 142:177-83. 2010
    ....
  11. ncbi request reprint Development and validation of an immunohistochemical method for rapid diagnosis of swine erysipelas in formalin-fixed, paraffin-embedded tissue samples
    Tanja Opriessnig
    Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    J Vet Diagn Invest 22:86-90. 2010
    ..In addition, the IHC was found to be useful for detection of E. rhusiopathiae antigen in skin lesions, which are often culture negative...
  12. doi request reprint Difference in severity of porcine circovirus type two-induced pathological lesions between Landrace and Pietrain pigs
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA
    J Anim Sci 87:1582-90. 2009
    ..The results from this study indicate that a genetic difference exists between these 2 breeds of pigs in susceptibility to PCV2-associated lesions...
  13. pmc Porcine circovirus type 2 (PCV2)-infection and re-inoculation with homologous or heterologous strains: virological, serological, pathological and clinical effects in growing pigs
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA
    Vet Res 41:31. 2010
    ..In the current study, pigs had extended viremia combined with detectable tissue PCV2 antigen levels despite the presence of high levels of anti-PCV2 antibody; however, no clinical disease was observed...
  14. doi request reprint Experimental reproduction of porcine circovirus type 2 (PCV2)-associated enteritis in pigs infected with PCV2 alone or concurrently with Lawsonia intracellularis or Salmonella typhimurium
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA
    J Comp Pathol 145:261-70. 2011
    ....
  15. doi request reprint Porcine circovirus type 2 (PCV2) vaccination is effective in reducing disease and PCV2 shedding in semen of boars concurrently infected with PCV2 and Mycoplasma hyopneumoniae
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa, USA
    Theriogenology 76:351-60. 2011
    ..In summary, although concurrent MHYO infection did not influence PCV2 shedding patterns, coinfection of boars with PCV2 and MHYO resulted in severe clinical disease and viral shedding was significantly decreased by PCV2 vaccination...
  16. pmc A live-attenuated chimeric porcine circovirus type 2 (PCV2) vaccine is transmitted to contact pigs but is not upregulated by concurrent infection with porcine parvovirus (PPV) and porcine reproductive and respiratory syndrome virus (PRRSV) and is efficaci
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Clin Vaccine Immunol 18:1261-8. 2011
    ....
  17. pmc Limited susceptibility of three different mouse (Mus musculus) lines to Porcine circovirus-2 infection and associated lesions
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA
    Can J Vet Res 73:81-6. 2009
    ..The results indicate the mouse model likely has limited utility to advance understanding of the pathogenesis of PCV-2 associated lesions, but mice could potentially be important in the epidemiology of PCV-2...
  18. doi request reprint Polymicrobial respiratory disease in pigs
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, USA
    Anim Health Res Rev 12:133-48. 2011
    ..This review discusses the latest findings on polymicrobial respiratory disease in pigs...
  19. doi request reprint Effect of porcine circovirus type 2 (PCV2) vaccination on porcine reproductive and respiratory syndrome virus (PRRSV) and PCV2 coinfection
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Vet Microbiol 131:103-14. 2008
    ..Differences between intradermal and intramuscular routes of vaccine administration were not observed...
  20. pmc Influence of maternal antibodies on efficacy of porcine circovirus type 2 (PCV2) vaccination to protect pigs from experimental infection with PCV2
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Clin Vaccine Immunol 15:397-401. 2008
    ..These results indicate that vaccination with Suvaxyn PCV2 One Dose reduces viremia and prevents microscopic lesions associated with PCV2 in the presence of maternal antibodies...
  21. doi request reprint Differences in virulence among porcine circovirus type 2 isolates are unrelated to cluster type 2a or 2b and prior infection provides heterologous protection
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    J Gen Virol 89:2482-91. 2008
    ..Increased virulence as reported to be associated with PCV2b isolates in the field was not observed under the conditions of this study. Moreover, cross-protection between PCV2a and PCV2b exists...
  22. pmc Use of an experimental model to test the efficacy of planned exposure to live porcine reproductive and respiratory syndrome virus
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Clin Vaccine Immunol 14:1572-7. 2007
    ..Planned exposure to live PRRSV can be used as an inexpensive and effective way to decrease the severity of PRRSV-induced disease following subsequent challenge...
  23. ncbi request reprint Genetic and experimental comparison of porcine circovirus type 2 (PCV2) isolates from cases with and without PCV2-associated lesions provides evidence for differences in virulence
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011 1250, USA
    J Gen Virol 87:2923-32. 2006
    ..05) in pigs inoculated with PCV2-4838. The results of this study suggest that PCV2 isolates from the USA differ in virulence in an SPF pig model...
  24. doi request reprint Comparison of efficacy of commercial one dose and two dose PCV2 vaccines using a mixed PRRSV-PCV2-SIV clinical infection model 2-3-months post vaccination
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, United States
    Vaccine 27:1002-7. 2009
    ..Viremia was reduced by 78.5% in pigs vaccinated with one dose and by 97.1% in pigs vaccinated with two dose products and overall microscopic lymphoid lesions were reduced by 78.7% and 81.8%, respectively...
  25. pmc Porcine circovirus type 2 infection decreases the efficacy of a modified live porcine reproductive and respiratory syndrome virus vaccine
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Clin Vaccine Immunol 13:923-9. 2006
    ..001) lower average daily weight gain than did the control and the vaccinated groups. This work suggests that PCV2 infection has an adverse effect on the development of protective immunity induced by PRRSV vaccine...
  26. doi request reprint Porcine circovirus type 2 in muscle and bone marrow is infectious and transmissible to naïve pigs by oral consumption
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1600 South 16th Street, Ames, IA 50011, USA
    Vet Microbiol 133:54-64. 2009
    ..Results from this study indicate that uncooked PCV2 DNA positive lymphoid tissues, bone marrow, and skeletal muscle from PCV2 viremic pigs contain sufficient amount of infectious PCV2 to infect naïve pigs by the oral route...
  27. pmc Studies on porcine circovirus type 2 vaccination of 5-day-old piglets
    K C O'Neill
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1600 S 16th Street, Ames, IA 50011, USA
    Clin Vaccine Immunol 18:1865-71. 2011
    ....
  28. doi request reprint Identification and characterization of novel porcine astroviruses (PAstVs) with high prevalence and frequent co-infection of individual pigs with multiple PAstV types
    Chao Ting Xiao
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA
    J Gen Virol 94:570-82. 2013
    ..Phylogenetic analysis revealed that PAstV1, PAstV2 and PAstV3 were more closely related to AstVs from humans and other animals than to each other, indicating past cross-species transmission and the zoonotic potential of these PAstVs...
  29. ncbi request reprint Characterization of shedding patterns of Porcine circovirus types 2a and 2b in experimentally inoculated mature boars
    Darin M Madson
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    J Vet Diagn Invest 20:725-34. 2008
    ..Differences in shedding patterns between PCV-2a and -2b were not observed under the study conditions...
  30. pmc Infectivity of porcine circovirus type 2 DNA in semen from experimentally-infected boars
    Darin M Madson
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA
    Vet Res 40:10. 2009
    ....
  31. doi request reprint ORF1 but not ORF2 dependent differences are important for in vitro replication of PCV2 in porcine alveolar macrophages singularly or coinfected with PRRSV
    Avanti Sinha
    Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011 1250, USA
    Vet Microbiol 158:95-103. 2012
    ....
  32. doi request reprint Prevalence and phylogenetic analysis of the current porcine circovirus 2 genotypes after implementation of widespread vaccination programmes in the USA
    Hui Gang Shen
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA
    J Gen Virol 93:1345-55. 2012
    ..The data generated in this study provide novel information on the distribution of PCV2 genotypes in vaccinated pig populations...
  33. doi request reprint Expression of human CD46 has no effect on porcine circovirus type 2 infection and shedding in the experimental pig model
    Michelle Hemann
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1600 S 16th Street, Ames, IA, 50011, USA
    Vet Res Commun 36:187-93. 2012
    ..Under the study conditions, the presence of human CD46 in transgenic pigs had no effect on PCV2 infection in otherwise healthy pigs capable of a normal immune response...
  34. ncbi request reprint Comparison of three enzyme-linked immunosorbent assays to detect Porcine circovirus-2 (PCV-2)-specific antibodies after vaccination or inoculation of pigs with distinct PCV-1 or PCV-2 isolates
    Abby R Patterson
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011 1250, USA
    J Vet Diagn Invest 20:744-51. 2008
    ..One of the ELISAs was able to distinguish pigs vaccinated with vaccine B from pigs inoculated with either PCV-2a or PCV-2b...
  35. doi request reprint Novel chimeric porcine circovirus (PCV) with the capsid gene of the emerging PCV2b subtype cloned in the genomic backbone of the non-pathogenic PCV1 is attenuated in vivo and induces protective and cross-protective immunity against PCV2b and PCV2a subtype
    Nathan M Beach
    Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States
    Vaccine 29:221-32. 2010
    ..The results indicate the chimeric PCV1-2b virus is a good candidate for a live-attenuated vaccine against both PCV2b and PCV2a subtypes...
  36. doi request reprint Characterization of porcine parvovirus type 2 (PPV2) which is highly prevalent in the USA
    Chao Ting Xiao
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, United States
    Vet Microbiol 161:325-30. 2013
    ..Phylogenetic and sequence homology analyses demonstrated a close relationship of PPV2 with members of the proposed genus PARV4-like virus, and the classification of PPV2 into this proposed genus is suggested...
  37. pmc Comparison of commercial real-time reverse transcription-PCR assays for reliable, early, and rapid detection of heterologous strains of porcine reproductive and respiratory syndrome virus in experimentally infected or noninfected boars by use of different
    Priscilla F Gerber
    Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa, USA
    J Clin Microbiol 51:547-56. 2013
    ..The AB assay had a 1.6-times-shorter PCR time. In summary, serum and blood swabs had the best performance with highest detection rates and agreement between assays and the shortest turnaround times...
  38. doi request reprint High prevalence of porcine circovirus viremia in newborn piglets in five clinically normal swine breeding herds in North America
    Huigang Shen
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Prev Vet Med 97:228-36. 2010
    ..This information adds to the knowledge of PCV2 infection in breeding herds...
  39. pmc Serological profile of torque teno sus virus species 1 (TTSuV1) in pigs and antigenic relationships between two TTSuV1 genotypes (1a and 1b), between two species (TTSuV1 and -2), and between porcine and human anelloviruses
    Yao Wei Huang
    Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA
    J Virol 86:10628-39. 2012
    ..These results have important implications for an understanding of the diversity of anelloviruses as well as for the classification and vaccine development of TTSuVs...
  40. doi request reprint Cytokine and chemokine mRNA expression profiles in tracheobronchial lymph nodes from pigs singularly infected or coinfected with porcine circovirus type 2 (PCV2) and Mycoplasma hyopneumoniae (MHYO)
    Huanrong Zhang
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1600 S 16th Street, Ames, IA 50011, USA
    Vet Immunol Immunopathol 140:152-8. 2011
    ..In summary, this work provided evidence that the increased severity of lesions in PCV2 and MHYO coinfected pigs was associated mainly with the presence of PCV2 antigen and alterations of cytokine mRNA expression profiles...
  41. ncbi request reprint Comparison of conventional direct and enrichment culture methods for Erysipelothrix spp. from experimentally and naturally infected swine
    Joseph S Bender
    Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    J Vet Diagn Invest 21:863-8. 2009
    ..from clinical samples with a history suggestive of erysipelas and that Vagococcus spp. is potentially an important differential diagnosis...
  42. doi request reprint Increasing porcine PARV4 prevalence with pig age in the U.S. pig population
    Chao Ting Xiao
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA
    Vet Microbiol 160:290-6. 2012
    ..To the authors' knowledge, this is the first description of porcine PARV4 in the U.S. pig population...
  43. ncbi request reprint Erysipelothrix spp. genotypes, serotypes, and surface protective antigen types associated with abattoir condemnations
    Joseph S Bender
    Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    J Vet Diagn Invest 23:139-42. 2011
    ..tonsillarum, thought to be avirulent for swine, was isolated from systemic sites from 3.4% of the carcasses that were negative for E. rhusiopathiae, indicating the potential importance of this genotype in erysipelas pathogenesis...
  44. doi request reprint The prevalence of Torque teno sus virus (TTSuV) is common and increases with the age of growing pigs in the United States
    Chao Ting Xiao
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA
    J Virol Methods 183:40-4. 2012
    ..8% and 100% in growing pigs. The results of this study indicate that vertical transmission may not be a main route of TTSuV transmission in pigs in the USA...
  45. pmc Characterization of a novel porcine parvovirus tentatively designated PPV5
    Chao Ting Xiao
    Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa, United States of America
    PLoS ONE 8:e65312. 2013
    ..The high similarity to PPV4 and the lack of ORF3 may suggest PPV5 is an intermediate of PPV4 during the evolution of parvoviruses in pigs...
  46. ncbi request reprint Interlaboratory comparison of Porcine circovirus-2 indirect immunofluorescent antibody test and enzyme-linked immunosorbent assay results on experimentally infected pigs
    Abby R Patterson
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    J Vet Diagn Invest 23:206-12. 2011
    ..94 to 1.00, 0.72 to 1.00, and 0.95 to 1.00, respectively. However, test sensitivities varied based on laboratory-specific cutoffs that were used to dichotomize test results...
  47. doi request reprint Genetic and phenotypic characterization of a 2006 United States porcine reproductive and respiratory virus isolate associated with high morbidity and mortality in the field
    Phillip C Gauger
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Virus Res 163:98-107. 2012
    ....
  48. doi request reprint Inhibition of porcine reproductive and respiratory syndrome virus infection in piglets by a peptide-conjugated morpholino oligomer
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, USA
    Antiviral Res 91:36-42. 2011
    ..This study provides further data indicating that the 5UP2 PPMO can be considered a candidate component for a novel PRRS control strategy...
  49. pmc Complete genome sequence of a newly identified porcine astrovirus genotype 3 strain US-MO123
    Chao Ting Xiao
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA
    J Virol 86:13126. 2012
    ..Sequence comparison and phylogenetic analysis showed that PAstV3 has the closest relationship with mink AstV and the human AstV strains VA1, VA2, and SG, indicating the same ancestral origin and zoonotic potential of the virus...
  50. pmc Complete genome sequence of a novel porcine circovirus type 2b variant present in cases of vaccine failures in the United States
    Chao Ting Xiao
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA
    J Virol 86:12469. 2012
    ..The genomic knowledge of this mutant PCV2b will improve understanding of the epidemiology of PCV and potentially inform the development of new and more effective vaccines for PCV2...
  51. pmc Comparison of experimental models for Streptococcus suis infection of conventional pigs
    Francisco J Pallares
    Veterinary Diagnostic Laboratory, Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA
    Can J Vet Res 67:225-8. 2003
    ..The IN-AA model should serve as a good model for S. suis-induced disease, because the natural route of exposure is intranasal and the IN-AA model was effective in inducing disease that mimics what is observed in the field...
  52. doi request reprint Establishment of a DNA-launched infectious clone for a highly pneumovirulent strain of type 2 porcine reproductive and respiratory syndrome virus: identification and in vitro and in vivo characterization of a large spontaneous deletion in the nsp2 region
    Yan Yan Ni
    Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24061 0913, USA
    Virus Res 160:264-73. 2011
    ..The results from this study indicates that the spontaneous nsp2 deletion plays a role for enhanced PRRSV replication in vitro but has no effect on the pathogenicity of the virus...
  53. doi request reprint Comparison of commercial enzyme-linked immunosorbent assays and fluorescent microbead immunoassays for detection of antibodies against porcine reproductive and respiratory syndrome virus in boars
    Priscilla F Gerber
    Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA Laboratório de Pesquisa em Virologia Animal, Departamento de Medicina Veterinaria Preventiva, Universidade Federal de Minas Gerais, Minas Gerais, Brazil
    J Virol Methods 197:63-6. 2014
    ..In this study, the nucleocapsid protein utilized as antigen in the FMIAs yielded a low specificity. IDEXX-Se had the earliest detection and similar sensitivity and specificity to the HIPRA-Se. ..
  54. doi request reprint High prevalence and genetic diversity of porcine bocaviruses in pigs in the USA, and identification of multiple novel porcine bocaviruses
    Yong Hou Jiang
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA
    J Gen Virol 95:453-65. 2014
    ..Further studies will be required to reveal the possible pathogenic role of these diverse PBoVs. ..
  55. pmc A live-attenuated and an inactivated chimeric porcine circovirus (PCV)1-2 vaccine are both effective at inducing a humoral immune response and reducing PCV2 viremia and intrauterine infection in female swine of breeding age
    Michelle Hemann
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine Hemann, Wang, Halbur, Opriessnig and Department of Statistics, College of Liberal Arts and Sciences, Iowa State University, Ames, Iowa, USA Wang Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA Beach, Meng The Roslin Institute and The Royal Dick School of Veterinary Studies, University of Edinburgh, Midlothian, United Kingdom Opriessnig
    Can J Vet Res 78:8-16. 2014
    ..Under the conditions of this pilot study, both vaccines protected against PCV2 viremia in breeding age animals; however, vertical transmission was not prevented. ..
  56. doi request reprint Simultaneous detection of antibodies against Apx toxins ApxI, ApxII, ApxIII, and ApxIV in pigs with known and unknown Actinobacillus pleuropneumoniae exposure using a multiplexing liquid array platform
    Luis G Giménez-Lirola
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA
    Clin Vaccine Immunol 21:85-95. 2014
    ..5 to 56%; specificity, 100%) and is potentially an improved tool for the surveillance of disease and for monitoring vaccination compliance. ..
  57. doi request reprint Concurrent porcine circovirus type 2a (PCV2a) or PCV2b infection increases the rate of amino acid mutations of porcine reproductive and respiratory syndrome virus (PRRSV) during serial passages in pigs
    Shuang Hui Yin
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA
    Virus Res 178:445-51. 2013
    ..Furthermore, a significantly higher mutation rate of PRRSV was observed when pigs were co-infected with PCV2 highlighting the importance of concurrent infections on PRRSV evolution and control. ..
  58. doi request reprint Association of concurrent porcine circovirus (PCV) 2a and 2b infection with PCV associated disease in vaccinated pigs
    Priscilla F Gerber
    Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA
    Res Vet Sci 95:775-81. 2013
    ..The finding that only 50% of the pigs developed a detectable immune response to vaccination suggests poor vaccine compliance or efficacy. Concurrent PCV2a and PCV2b infection was common and may have resulted in enhanced PCV2 replication...
  59. doi request reprint Singular PCV2a or PCV2b infection results in apoptosis of hepatocytes in clinically affected gnotobiotic pigs
    Avanti Sinha
    Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA 50011, USA
    Res Vet Sci 92:151-6. 2012
    ..This suggests that PCV2-induced apoptosis of hepatocytes is important in the pathogenesis of PCV2-associated lesions and disease...
  60. doi request reprint Development of a fluorescent microbead-based immunoassay for the detection of hepatitis E virus IgG antibodies in pigs and comparison to an enzyme-linked immunoassay
    Olajide A Owolodun
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA Department of Biochemistry and Applied Molecular Biology, National Veterinary Research Institute, Vom, Plateau State, Nigeria
    J Virol Methods 193:278-83. 2013
    ..The overall prevalence of HEV IgG antibodies in field samples from pigs with unknown HEV exposure was 21.9% (40/182) for the ELISA and 21.4% (39/182) for the FMIA. The two assays had an almost perfect overall agreement (Kappa=0.92). ..
  61. pmc Rescue of a porcine anellovirus (torque teno sus virus 2) from cloned genomic DNA in pigs
    Yao Wei Huang
    Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic Institute and State University Virginia Tech, Blacksburg, Virginia, USA
    J Virol 86:6042-54. 2012
    ..The availability of an infectious DNA clone of TTSuV2 will facilitate future study of porcine anellovirus pathogenesis and biology...
  62. doi request reprint A commercial vaccine based on PCV2a and an experimental vaccine based on a variant mPCV2b are both effective in protecting pigs against challenge with a 2013 U.S. variant mPCV2b strain
    Tanja Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA The Roslin Institute and The Royal Dick School of Veterinary Studies, University of Edinburgh, Midlothian, United Kingdom Electronic address
    Vaccine 32:230-7. 2014
    ..Under the conditions of this study, PCV2 vaccines regardless of PCV2 type were effective against mPCV2b challenge. ..
  63. ncbi request reprint Development of a reverse transcription-PCR assay to detect porcine circovirus type 2 transcription as a measure of replication
    Shan Yu
    Department of Veterinary Microbiology and Preventive Medicine, Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    J Virol Methods 123:109-12. 2005
    ..This assay will be useful in assessing cell populations that support PCV2 replication in vivo or in vitro and advance the understanding of PCV2 replication and pathogenesis...
  64. ncbi request reprint Systemic Conidiobolus incongruus infection and hypertrophic osteopathy in a white-tailed deer (Odocoileus virginianus)
    Darin M Madson
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    J Vet Diagn Invest 21:167-70. 2009
    ..Furthermore, the proliferative lesions of the metatarsal bones along with the intrathoracic mass were compatible with hypertrophic osteopathy...
  65. pmc Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States
    Yao Wei Huang
    Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA
    MBio 4:e00737-13. 2013
    ..The data from this study have important implications for understanding the ongoing PEDV outbreaks in the United States and will guide future efforts to develop effective preventive and control measures against PEDV...
  66. doi request reprint Molecular evolutionary genetic analysis of emerging parvoviruses identified in pigs
    Chao Ting Xiao
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Infect Genet Evol 16:369-76. 2013
    ..The present analysis will assist to clarify the nomenclature of parvoviruses in pigs and facilitate future uniform assignment of names for new parvoviruses within the subfamily Parvovirinae...
  67. pmc Porcine reproductive and respiratory syndrome virus infection at the time of porcine circovirus type 2 vaccination has no impact on vaccine efficacy
    A Sinha
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Clin Vaccine Immunol 17:1940-5. 2010
    ..The results indicate that acute PRRSV infection at the time of PCV2 vaccination has no adverse effect on PCV2 vaccine efficacy...
  68. ncbi request reprint Peptide-conjugated morpholino oligomers inhibit porcine reproductive and respiratory syndrome virus replication
    Deendayal Patel
    Molecular Virology Laboratory, VA MD Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA
    Antiviral Res 77:95-107. 2008
    ..5UP2 and 5HP inhibited virus replication of 10 other strains of PRRSV. Results from this study suggest potential applications of PPMO for PRRS control...