KRISTEN JOHANSEN

Summary

Affiliation: Iowa State University
Country: USA

Publications

  1. ncbi request reprint Recent glimpses of the elusive spindle matrix
    Kristen M Johansen
    Department of Zoology and Genetics, Iowa State University, 3154 Molecular Biology Building, Ames, Iowa 50011, USA
    Cell Cycle 1:312-4. 2002
  2. pmc Evidence against a role for the JIL-1 kinase in H3S28 phosphorylation and 14-3-3 recruitment to active genes in Drosophila
    Chao Wang
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa, United States of America
    PLoS ONE 8:e62484. 2013
  3. pmc Skeletor, a novel chromosomal protein that redistributes during mitosis provides evidence for the formation of a spindle matrix
    D L Walker
    Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011, USA
    J Cell Biol 151:1401-12. 2000
  4. pmc JIL-1, a chromosomal kinase implicated in regulation of chromatin structure, associates with the male specific lethal (MSL) dosage compensation complex
    Y Jin
    Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011, USA
    J Cell Biol 149:1005-10. 2000
  5. ncbi request reprint Regulation of chromatin structure by histone H3S10 phosphorylation
    Kristen M Johansen
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, 3154 Molecular Biology Building, Ames, Iowa 50011, USA
    Chromosome Res 14:393-404. 2006
  6. pmc Reduced levels of Su(var)3-9 but not Su(var)2-5 (HP1) counteract the effects on chromatin structure and viability in loss-of-function mutants of the JIL-1 histone H3S10 kinase
    Huai Deng
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Genetics 177:79-87. 2007
  7. ncbi request reprint The lamin Dm0 allele Ari3 acts as an enhancer of position effect variegation of the wm4 allele in Drosophila
    Xiaomin Bao
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, 3154 Molecular Biology Building, Ames, Iowa 50011, USA
    Genetica 129:339-42. 2007
  8. pmc Polytene chromosome squash methods for studying transcription and epigenetic chromatin modification in Drosophila using antibodies
    Kristen M Johansen
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, 3154 Molecular Biology Building, Ames, IA 50011, USA
    Methods 48:387-97. 2009
  9. pmc Megator, an essential coiled-coil protein that localizes to the putative spindle matrix during mitosis in Drosophila
    Hongying Qi
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Mol Biol Cell 15:4854-65. 2004
  10. ncbi request reprint The JIL-1 histone H3S10 kinase regulates dimethyl H3K9 modifications and heterochromatic spreading in Drosophila
    Weiguo Zhang
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Development 133:229-35. 2006

Research Grants

  1. Regulation of Chromatin Structure byPhosphorylation
    KRISTEN JOHANSEN; Fiscal Year: 2004
  2. Regulation of chromatin structure and gene expression
    KRISTEN JOHANSEN; Fiscal Year: 2007
  3. Regulation of chromatin structure and gene expression
    Kristen M Johansen; Fiscal Year: 2010

Collaborators

Detail Information

Publications32

  1. ncbi request reprint Recent glimpses of the elusive spindle matrix
    Kristen M Johansen
    Department of Zoology and Genetics, Iowa State University, 3154 Molecular Biology Building, Ames, Iowa 50011, USA
    Cell Cycle 1:312-4. 2002
    ..Three recent papers suggest that the spindle matrix may be more than a theoretical idea...
  2. pmc Evidence against a role for the JIL-1 kinase in H3S28 phosphorylation and 14-3-3 recruitment to active genes in Drosophila
    Chao Wang
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa, United States of America
    PLoS ONE 8:e62484. 2013
    ..Thus, our results argue strongly against a model where JIL-1 is required for H3S28 phosphorylation and 14-3-3 recruitment at active genes...
  3. pmc Skeletor, a novel chromosomal protein that redistributes during mitosis provides evidence for the formation of a spindle matrix
    D L Walker
    Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011, USA
    J Cell Biol 151:1401-12. 2000
    ..Thus, the identification and characterization of skeletor represents the first direct molecular evidence for the existence of a complete spindle matrix that forms within the nucleus before microtubule spindle formation...
  4. pmc JIL-1, a chromosomal kinase implicated in regulation of chromatin structure, associates with the male specific lethal (MSL) dosage compensation complex
    Y Jin
    Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011, USA
    J Cell Biol 149:1005-10. 2000
    ..Thus, these results strongly indicate JIL-1 associates with the MSL complex and further suggests JIL-1 functions in signal transduction pathways regulating chromatin structure...
  5. ncbi request reprint Regulation of chromatin structure by histone H3S10 phosphorylation
    Kristen M Johansen
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, 3154 Molecular Biology Building, Ames, Iowa 50011, USA
    Chromosome Res 14:393-404. 2006
    ....
  6. pmc Reduced levels of Su(var)3-9 but not Su(var)2-5 (HP1) counteract the effects on chromatin structure and viability in loss-of-function mutants of the JIL-1 histone H3S10 kinase
    Huai Deng
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Genetics 177:79-87. 2007
    ....
  7. ncbi request reprint The lamin Dm0 allele Ari3 acts as an enhancer of position effect variegation of the wm4 allele in Drosophila
    Xiaomin Bao
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, 3154 Molecular Biology Building, Ames, Iowa 50011, USA
    Genetica 129:339-42. 2007
    ..These results indicate that lamins may be involved in regulating gene silencing and heterochromatic spreading at the wm4 locus and provide evidence that lamins may contribute to the regulation of higher-order chromatin organization...
  8. pmc Polytene chromosome squash methods for studying transcription and epigenetic chromatin modification in Drosophila using antibodies
    Kristen M Johansen
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, 3154 Molecular Biology Building, Ames, IA 50011, USA
    Methods 48:387-97. 2009
    ..Here we provide detailed protocols for polytene chromosome squash preparation and discuss their relative pros and cons in terms of suitability for reliable antibody labeling and preservation of high resolution chromatin structure...
  9. pmc Megator, an essential coiled-coil protein that localizes to the putative spindle matrix during mitosis in Drosophila
    Hongying Qi
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Mol Biol Cell 15:4854-65. 2004
    ....
  10. ncbi request reprint The JIL-1 histone H3S10 kinase regulates dimethyl H3K9 modifications and heterochromatic spreading in Drosophila
    Weiguo Zhang
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Development 133:229-35. 2006
    ..Based on these findings, we propose a model where JIL-1 kinase activity functions to maintain euchromatic regions by antagonizing Su(var)3-9-mediated heterochromatization...
  11. doi request reprint Ectopic histone H3S10 phosphorylation causes chromatin structure remodeling in Drosophila
    Huai Deng
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Development 135:699-705. 2008
    ..These findings provide direct evidence that the epigenetic histone tail modification of H3S10 phosphorylation at interphase can function as a causative regulator of higher-order chromatin structure in Drosophila in vivo...
  12. pmc RNA polymerase II-mediated transcription at active loci does not require histone H3S10 phosphorylation in Drosophila
    Weili Cai
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Development 135:2917-25. 2008
    ....
  13. doi request reprint Asator, a tau-tubulin kinase homolog in Drosophila localizes to the mitotic spindle
    Hongying Qi
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Dev Dyn 238:3248-56. 2009
    ..The finding that Asator localizes to the spindle region during mitosis and directly can interact with Megator suggests that its kinase activity may be involved in regulating microtubule dynamics and microtubule spindle function...
  14. pmc The COOH-terminal domain of the JIL-1 histone H3S10 kinase interacts with histone H3 and is required for correct targeting to chromatin
    Xiaomin Bao
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Biol Chem 283:32741-50. 2008
    ..Taken together these findings indicate that JIL-1 may participate in regulating chromatin structure by multiple and partially redundant mechanisms...
  15. pmc Loss-of-function alleles of the JIL-1 histone H3S10 kinase enhance position-effect variegation at pericentric sites in Drosophila heterochromatin
    Xiaomin Bao
    Department of Biochemistry, Biophysics, and Molecular Biology Iowa State University Ames, Iowa 50011, USA
    Genetics 176:1355-8. 2007
    ....
  16. ncbi request reprint Chromator, a novel and essential chromodomain protein interacts directly with the putative spindle matrix protein skeletor
    Uttama Rath
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Cell Biochem 93:1033-47. 2004
    ..These findings suggest that Chromator is a nuclear protein that plays a role in proper spindle dynamics during mitosis...
  17. ncbi request reprint The JIL-1 kinase interacts with lamin Dm0 and regulates nuclear lamina morphology of Drosophila nurse cells
    Xiaomin Bao
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    J Cell Sci 118:5079-87. 2005
    ..Thus, our results suggest that the JIL-1 kinase is required to maintain nuclear morphology and integrity of nurse cells during oogenesis and that this function may be linked to molecular interactions with lamin Dm0...
  18. ncbi request reprint The JIL-1 kinase regulates the structure of Drosophila polytene chromosomes
    Huai Deng
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, 3154 Molecular Biology Building, Ames, IA 50011, USA
    Chromosoma 114:173-82. 2005
    ....
  19. ncbi request reprint The chromodomain protein, Chromator, interacts with JIL-1 kinase and regulates the structure of Drosophila polytene chromosomes
    Uttama Rath
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, 50011, USA
    J Cell Sci 119:2332-41. 2006
    ..Taken together these findings indicate that Chromator and JIL-1 interact in an interband-specific complex that functions to establish or maintain polytene chromosome structure in Drosophila...
  20. ncbi request reprint The spindle matrix through the cell cycle in Drosophila
    Jørgen Johansen
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, USA
    Fly (Austin) 3:213-20. 2009
    ....
  21. doi request reprint Chromator is required for proper microtubule spindle formation and mitosis in Drosophila
    Yun Ding
    Department of Biochemistry, Biophysics, and Molecular Biology, 3156 Molecular Biology Building, Iowa State University, Ames, IA 50011, USA
    Dev Biol 334:253-63. 2009
    ....
  22. doi request reprint Chromatin structure and the regulation of gene expression: the lessons of PEV in Drosophila
    Jack R Girton
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Adv Genet 61:1-43. 2008
    ..Current studies of PEV are focusing on defining the complex patterns of modifier gene activity and the sequence of events that leads to the dynamic interplay between heterochromatin and euchromatin...
  23. ncbi request reprint EAST interacts with Megator and localizes to the putative spindle matrix during mitosis in Drosophila
    Hongying Qi
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Cell Biochem 95:1284-91. 2005
    ..On the basis of these findings, we propose that Megator and EAST interact to form a nuclear endoskeleton and as well are important components of the putative spindle matrix complex during mitosis...
  24. ncbi request reprint Studying nuclear organization in embryos using antibody tools
    Kristen M Johansen
    Department of Biochemistry, Iowa State University, Ames, IA, USA
    Methods Mol Biol 247:215-34. 2004
  25. pmc Genetic and phenotypic analysis of alleles of the Drosophila chromosomal JIL-1 kinase reveals a functional requirement at multiple developmental stages
    Weiguo Zhang
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Genetics 165:1341-54. 2003
    ..We propose that JIL-1 may exert such effects by a general regulation of chromatin structure affecting gene expression...
  26. pmc Loss-of-function alleles of the JIL-1 kinase are strong suppressors of position effect variegation of the wm4 allele in Drosophila
    Stephanie Lerach
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Genetics 173:2403-6. 2006
    ....
  27. ncbi request reprint D-Hillarin, a novel W180-domain protein, affects cytokinesis through interaction with the septin family member Pnut
    Yun Ji
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Neurobiol 64:157-69. 2005
    ..Based on these results we propose that D-hil may function as a regulator of septin function during cytokinesis in the developing nervous system...
  28. ncbi request reprint Cell and molecular biology of the spindle matrix
    Kristen M Johansen
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Int Rev Cytol 263:155-206. 2007
    ..However, as reviewed here multiple studies spanning the evolutionary spectrum from lower eukaryotes to vertebrates have provided new and intriguing evidence that a spindle matrix may be a general feature of mitosis...
  29. ncbi request reprint A developmentally regulated splice variant from the complex lola locus encoding multiple different zinc finger domain proteins interacts with the chromosomal kinase JIL-1
    Weiguo Zhang
    Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011, USA
    J Biol Chem 278:11696-704. 2003
    ..Thus, genetic interaction assays provide direct evidence that gene products from the lola locus function within the same pathway as the chromosomal kinase JIL-1...
  30. pmc JIL-1 kinase, a member of the male-specific lethal (MSL) complex, is necessary for proper dosage compensation of eye pigmentation in Drosophila
    Stephanie Lerach
    Genesis 43:213-5. 2005
    ..These results indicate that proper dosage compensation of eye pigment levels in males controlled by X-linked white alleles requires normal JIL-1 function...
  31. ncbi request reprint Titin in insect spermatocyte spindle fibers associates with microtubules, actin, myosin and the matrix proteins skeletor, megator and chromator
    Lacramioara Fabian
    Biology Department, York University, Toronto, ON, Canada
    J Cell Sci 120:2190-204. 2007
    ..The varying spatial arrangements of these proteins during the course of division suggest that they interact to form a spindle matrix with elastic properties provided by a titin-like protein...

Research Grants8

  1. Regulation of Chromatin Structure byPhosphorylation
    KRISTEN JOHANSEN; Fiscal Year: 2004
    ..abstract_text> ..
  2. Regulation of chromatin structure and gene expression
    KRISTEN JOHANSEN; Fiscal Year: 2007
    ..Thus, the proposed studies of JIL-1 will provide important new insights into the molecular mechanisms of how kinase activity modulates chromatin structure and gene regulation that are directly relevant to humans. ..
  3. Regulation of chromatin structure and gene expression
    Kristen M Johansen; Fiscal Year: 2010
    ..Thus, the proposed studies of JIL-1 will provide important new insights into the molecular mechanisms of how kinase activity modulates chromatin structure and gene regulation that are directly relevant to humans. ..