Karen Harmon

Summary

Affiliation: Iowa State University
Country: USA

Publications

  1. pmc A matrix gene-based multiplex real-time RT-PCR for detection and differentiation of 2009 pandemic H1N1 and other influenza A viruses in North America
    Karen Harmon
    Veterinary Diagnostic Laboratory, Iowa State University, Ames, IA 50011, USA
    Influenza Other Respir Viruses 4:405-10. 2010
  2. doi request reprint Porcine reproductive and respiratory syndrome virus (PRRSV) surveillance using pre-weaning oral fluid samples detects circulation of wild-type PRRSV
    Apisit Kittawornrat
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Vet Microbiol 168:331-9. 2014
  3. pmc Comparison of commercial real-time reverse transcription-PCR assays for reliable, early, and rapid detection of heterologous strains of porcine reproductive and respiratory syndrome virus in experimentally infected or noninfected boars by use of different
    Priscilla F Gerber
    Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa, USA
    J Clin Microbiol 51:547-56. 2013
  4. pmc Influenza A pandemic (H1N1) 2009 virus infection in domestic cat
    Brett A Sponseller
    College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011 1248, USA
    Emerg Infect Dis 16:534-7. 2010
  5. doi request reprint PCR-based retrospective evaluation of diagnostic samples for emergence of porcine deltacoronavirus in US swine
    Avanti Sinha
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA Electronic address
    Vet Microbiol 179:296-8. 2015
  6. ncbi request reprint Comparison of RNA extraction and real-time reverse transcription polymerase chain reaction methods for the detection of Porcine reproductive and respiratory syndrome virus in porcine oral fluid specimens
    Wayne A Chittick
    Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State University, 1600 South 16th Street, Ames, IA 50011 1250, USA
    J Vet Diagn Invest 23:248-53. 2011
  7. ncbi request reprint Ring test evaluation of the detection of influenza A virus in swine oral fluids by real-time reverse-transcription polymerase chain reaction and virus isolation
    Christa K Goodell
    Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute Building 1, 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011 1240, USA Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman Department of Statistics, Iowa State University, Ames, Iowa 50011, USA Wang Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St Paul, Minnesota 55108, USA Patnayak, Otterson, Culhane Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA Christopher Hennings, Clement, Leslie Steen Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA Hesse, Anderson, Skarbek US Department of Agriculture USDA National Animal Disease Center, Ames
    Can J Vet Res 80:12-20. 2016

Collaborators

Detail Information

Publications7

  1. pmc A matrix gene-based multiplex real-time RT-PCR for detection and differentiation of 2009 pandemic H1N1 and other influenza A viruses in North America
    Karen Harmon
    Veterinary Diagnostic Laboratory, Iowa State University, Ames, IA 50011, USA
    Influenza Other Respir Viruses 4:405-10. 2010
    ..The emergence in humans of pandemic (H1N1) 2009 (pH1N1) with similarities to swine influenza viruses (SIVs) caused much concern for both human and animal health as potential for interspecies transmission was initially unknown...
  2. doi request reprint Porcine reproductive and respiratory syndrome virus (PRRSV) surveillance using pre-weaning oral fluid samples detects circulation of wild-type PRRSV
    Apisit Kittawornrat
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Vet Microbiol 168:331-9. 2014
    ..0.98; p<0.001) S/P ratios. Overall, the study showed that pre-weaning litter oral fluid samples could provide an efficient and sensitive approach to surveil for PRRSV in infected, vaccinated, or presumed-negative pig breeding herds...
  3. pmc Comparison of commercial real-time reverse transcription-PCR assays for reliable, early, and rapid detection of heterologous strains of porcine reproductive and respiratory syndrome virus in experimentally infected or noninfected boars by use of different
    Priscilla F Gerber
    Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa, USA
    J Clin Microbiol 51:547-56. 2013
    ..The AB assay had a 1.6-times-shorter PCR time. In summary, serum and blood swabs had the best performance with highest detection rates and agreement between assays and the shortest turnaround times...
  4. pmc Influenza A pandemic (H1N1) 2009 virus infection in domestic cat
    Brett A Sponseller
    College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011 1248, USA
    Emerg Infect Dis 16:534-7. 2010
    ..In 2009, pandemic (H1N1) 2009 infection in a domestic cat from Iowa was diagnosed by a novel PCR assay that distinguishes between Eurasian and North American pandemic (H1N1) 2009 virus matrix genes. Human-to-cat transmission is presumed...
  5. doi request reprint PCR-based retrospective evaluation of diagnostic samples for emergence of porcine deltacoronavirus in US swine
    Avanti Sinha
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA Electronic address
    Vet Microbiol 179:296-8. 2015
    ..Therefore, with available samples submitted to the ISU VDL, it can be inferred that PDCoV has been present in US swine at least since August 2013. ..
  6. ncbi request reprint Comparison of RNA extraction and real-time reverse transcription polymerase chain reaction methods for the detection of Porcine reproductive and respiratory syndrome virus in porcine oral fluid specimens
    Wayne A Chittick
    Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State University, 1600 South 16th Street, Ames, IA 50011 1250, USA
    J Vet Diagn Invest 23:248-53. 2011
    ..The results suggest that further optimization of PCR assays for porcine oral fluids is needed and that laboratories should not assume that methods optimized for detection of virus in serum will perform equally with porcine oral fluids...
  7. ncbi request reprint Ring test evaluation of the detection of influenza A virus in swine oral fluids by real-time reverse-transcription polymerase chain reaction and virus isolation
    Christa K Goodell
    Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute Building 1, 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011 1240, USA Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman Department of Statistics, Iowa State University, Ames, Iowa 50011, USA Wang Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St Paul, Minnesota 55108, USA Patnayak, Otterson, Culhane Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA Christopher Hennings, Clement, Leslie Steen Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA Hesse, Anderson, Skarbek US Department of Agriculture USDA National Animal Disease Center, Ames
    Can J Vet Res 80:12-20. 2016
    ..Successful swine health monitoring and disease surveillance require assays with consistent performance, but significant differences in reproducibility were observed among the assays evaluated. ..