G M Culver

Summary

Affiliation: Iowa State University
Country: USA

Publications

  1. ncbi request reprint Meanderings of the mRNA through the ribosome
    G M Culver
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, 4216 Molecular Biology Building, Ames, IA 50011, USA
    Structure 9:751-8. 2001
  2. ncbi request reprint Assembly of the 30S ribosomal subunit
    Gloria M Culver
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University Ames, IA 50011, USA
    Biopolymers 68:234-49. 2003
  3. pmc Assembly of the 5' and 3' minor domains of 16S ribosomal RNA as monitored by tethered probing from ribosomal protein S20
    Laura M Dutca
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Mol Biol 376:92-108. 2008
  4. ncbi request reprint Analysis of conformational changes in 16 S rRNA during the course of 30 S subunit assembly
    Kristi L Holmes
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    J Mol Biol 354:340-57. 2005
  5. pmc Assembly of the 30S ribosomal subunit: positioning ribosomal protein S13 in the S7 assembly branch
    Joel F Grondek
    Department of Biochemistry, Biophysics and Molecular Biology, 4216 Molecular Biology Bldg, Iowa State University, Ames, IA 50011, USA
    RNA 10:1861-6. 2004
  6. ncbi request reprint Ribosomal protein-dependent orientation of the 16 S rRNA environment of S15
    Indu Jagannathan
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, 4258 Molecular Biology Building, Ames, IA 50011, USA
    J Mol Biol 335:1173-85. 2004
  7. ncbi request reprint Assembly of the central domain of the 30S ribosomal subunit: roles for the primary binding ribosomal proteins S15 and S8
    Indu Jagannathan
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, 4258 Molecular Biology Building, Ames, IA 50011, USA
    J Mol Biol 330:373-83. 2003
  8. ncbi request reprint The DnaK chaperone system facilitates 30S ribosomal subunit assembly
    Jennifer A Maki
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames 50011, USA
    Mol Cell 10:129-38. 2002
  9. pmc Demonstration of the role of the DnaK chaperone system in assembly of 30S ribosomal subunits using a purified in vitro system
    Jennifer A Maki
    Iowa State University, Department of Biochemistry, Biophysics, and Molecular Biology, Ames, Iowa 50011, USA
    RNA 9:1418-21. 2003
  10. ncbi request reprint Sno-capped: 5' ends of preribosomal RNAs are decorated with a U3 SnoRNP
    Gloria M Culver
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Chem Biol 9:777-9. 2002

Collaborators

  • Jennifer A Maki
  • Indu Jagannathan
  • Laura M Dutca
  • Kristi L Holmes
  • Jason P Rife
  • Joel F Grondek
  • Zhili Xu
  • Narayanaswamy Kirthi
  • Heather C O'Farrell
  • Biswajoy Roy-Chaudhuri
  • Teresa Kelley
  • Daniel R Southworth
  • Daniel J Schnobrich

Detail Information

Publications16

  1. ncbi request reprint Meanderings of the mRNA through the ribosome
    G M Culver
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, 4216 Molecular Biology Building, Ames, IA 50011, USA
    Structure 9:751-8. 2001
    ..The mechanisms and mechanics of reading frame selection, translational fidelity, and translocation can now be informed by the structure...
  2. ncbi request reprint Assembly of the 30S ribosomal subunit
    Gloria M Culver
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University Ames, IA 50011, USA
    Biopolymers 68:234-49. 2003
    ..In contrast, many details of how these massive ribonucleoprotein complexes assemble remain elusive. The goal of this review is to discuss some crucial aspects of 30S ribosomal subunit assembly...
  3. pmc Assembly of the 5' and 3' minor domains of 16S ribosomal RNA as monitored by tethered probing from ribosomal protein S20
    Laura M Dutca
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Mol Biol 376:92-108. 2008
    ..These results are similar to what would be predicted of subunit assembly by the 5'-to-3' direction assembly model...
  4. ncbi request reprint Analysis of conformational changes in 16 S rRNA during the course of 30 S subunit assembly
    Kristi L Holmes
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    J Mol Biol 354:340-57. 2005
    ..This work has allowed a more comprehensive and global view of E.coli 30 S ribosomal subunit assembly to be obtained...
  5. pmc Assembly of the 30S ribosomal subunit: positioning ribosomal protein S13 in the S7 assembly branch
    Joel F Grondek
    Department of Biochemistry, Biophysics and Molecular Biology, 4216 Molecular Biology Bldg, Iowa State University, Ames, IA 50011, USA
    RNA 10:1861-6. 2004
    ..These results link S13 to the 3' major domain family of proteins, and the S7 assembly branch, placing S13 in a new location in the 30S subunit assembly map where its position is in accordance with much biochemical and structural data...
  6. ncbi request reprint Ribosomal protein-dependent orientation of the 16 S rRNA environment of S15
    Indu Jagannathan
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, 4258 Molecular Biology Building, Ames, IA 50011, USA
    J Mol Biol 335:1173-85. 2004
    ..These studies reveal that ribosomal proteins can alter the assembly of regions of the 30 S subunit from a considerable distance and influence the overall conformation of this ribonucleoprotein particle...
  7. ncbi request reprint Assembly of the central domain of the 30S ribosomal subunit: roles for the primary binding ribosomal proteins S15 and S8
    Indu Jagannathan
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, 4258 Molecular Biology Building, Ames, IA 50011, USA
    J Mol Biol 330:373-83. 2003
    ..Thus, while S8 binding is not absolutely required for assembly of the platform, it appears to affect significantly the 16S rRNA environment of S15 by influencing central domain organization...
  8. ncbi request reprint The DnaK chaperone system facilitates 30S ribosomal subunit assembly
    Jennifer A Maki
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames 50011, USA
    Mol Cell 10:129-38. 2002
    ..These studies reveal a novel role for the DnaK/hsp70 chaperone system, in addition to its well-documented role in protein folding, and suggest that 30S subunit assembly can be facilitated...
  9. pmc Demonstration of the role of the DnaK chaperone system in assembly of 30S ribosomal subunits using a purified in vitro system
    Jennifer A Maki
    Iowa State University, Department of Biochemistry, Biophysics, and Molecular Biology, Ames, Iowa 50011, USA
    RNA 9:1418-21. 2003
    ..Here, we present additional data indicating that purified DnaK chaperone assembled 30S subunits are functional. Additionally, explanations for reported differences are discussed...
  10. ncbi request reprint Sno-capped: 5' ends of preribosomal RNAs are decorated with a U3 SnoRNP
    Gloria M Culver
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Chem Biol 9:777-9. 2002
    ..Work recently published in Nature demonstrates that a large ribonucleoprotein processes 18S rRNA in S. cerevisiae, indicating that macromolecular particles regulate ribosome maturation and revealing the complex nature of this process...
  11. pmc Temperature-dependent RNP conformational rearrangements: analysis of binary complexes of primary binding proteins with 16 S rRNA
    Laura M Dutca
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    J Mol Biol 368:853-69. 2007
    ..The differential interaction of 16 S rRNA with r-proteins illustrates a means for controlling the sequential assembly pathway for complex RNPs and may offer insights into aspects of RNP assembly in general...
  12. pmc A novel single amino acid change in small subunit ribosomal protein S5 has profound effects on translational fidelity
    Narayanaswamy Kirthi
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    RNA 12:2080-91. 2006
    ..This study highlights the importance of S5 in ribosome function and cell physiology, and suggests that translational fidelity can be regulated in multiple ways...
  13. ncbi request reprint Recent developments in factor-facilitated ribosome assembly
    Jennifer A Maki
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Methods 36:313-20. 2005
    ..Also, recent efforts toward understanding the roles of exogenous factors in 50S subunit and eukaryotic ribosome assembly will be briefly discussed...
  14. ncbi request reprint Mapping structural differences between 30S ribosomal subunit assembly intermediates
    Kristi L Holmes
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Nat Struct Mol Biol 11:179-86. 2004
    ....
  15. ncbi request reprint Breaking the cycle of translation
    Jason P Rife
    Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, VA 23298, USA
    Mol Cell 28:517-9. 2007
    ..Now, in this issue, the Green research group establishes unexpected complexity in decoding translation stop codons (Youngman et al., 2007)...
  16. doi request reprint A conserved rRNA methyltransferase regulates ribosome biogenesis
    Zhili Xu
    Department of Biology, Hutchison Hall 301, University of Rochester, Rochester, New York 14627, USA
    Nat Struct Mol Biol 15:534-6. 2008
    ..These findings suggest a checkpoint role for this modification system and offer a functional rationale for the unprecedented level of conservation...