Thomas A Bobik

Summary

Affiliation: Iowa State University
Country: USA

Publications

  1. ncbi request reprint Polyhedral organelles compartmenting bacterial metabolic processes
    Thomas A Bobik
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Appl Microbiol Biotechnol 70:517-25. 2006
  2. pmc Interactions between the termini of lumen enzymes and shell proteins mediate enzyme encapsulation into bacterial microcompartments
    Chenguang Fan
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Proc Natl Acad Sci U S A 109:14995-5000. 2012
  3. pmc The PduM protein is a structural component of the microcompartments involved in coenzyme B(12)-dependent 1,2-propanediol degradation by Salmonella enterica
    Sharmistha Sinha
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa, USA
    J Bacteriol 194:1912-8. 2012
  4. pmc Genetic analysis of the protein shell of the microcompartments involved in coenzyme B12-dependent 1,2-propanediol degradation by Salmonella
    Shouqiang Cheng
    Iowa State University, Department of Biochemistry, Biophysics and Molecular Biology, Ames, IA 50011, USA
    J Bacteriol 193:1385-92. 2011
  5. pmc Short N-terminal sequences package proteins into bacterial microcompartments
    Chenguang Fan
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Proc Natl Acad Sci U S A 107:7509-14. 2010
  6. pmc Kinetic and functional analysis of L-threonine kinase, the PduX enzyme of Salmonella enterica
    Chenguang Fan
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Biol Chem 284:20240-8. 2009
  7. pmc Structural insight into the mechanisms of transport across the Salmonella enterica Pdu microcompartment shell
    Christopher S Crowley
    Molecular Biology Institute, Iowa State University, Ames, Iowa 50011, USA
    J Biol Chem 285:37838-46. 2010
  8. pmc Characterization of the PduS cobalamin reductase of Salmonella enterica and its role in the Pdu microcompartment
    Shouqiang Cheng
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    J Bacteriol 192:5071-80. 2010
  9. pmc Bacterial microcompartments: their properties and paradoxes
    Shouqiang Cheng
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA
    Bioessays 30:1084-95. 2008
  10. pmc The N-terminal region of the medium subunit (PduD) packages adenosylcobalamin-dependent diol dehydratase (PduCDE) into the Pdu microcompartment
    Chenguang Fan
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Bacteriol 193:5623-8. 2011

Collaborators

  • Yu Liu
  • TODD YEATES
  • Ramon Gonzalez
  • Chenguang Fan
  • Shouqiang Cheng
  • Sharmistha Sinha
  • Christopher S Crowley
  • Huilin Zhu
  • David S Gogerty
  • Edith M Sampson
  • Duilio Cascio
  • Robert E Jefferson
  • Jeffery S Kopstein
  • Cristina M Escobar
  • Michael R Sawaya
  • Herbert J Fromm

Detail Information

Publications16

  1. ncbi request reprint Polyhedral organelles compartmenting bacterial metabolic processes
    Thomas A Bobik
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Appl Microbiol Biotechnol 70:517-25. 2006
    ..Therefore, the unusual structure of polyhedral organelles raises intriguing questions about their assembly, turnover, and molecular evolution, very little of which is understood...
  2. pmc Interactions between the termini of lumen enzymes and shell proteins mediate enzyme encapsulation into bacterial microcompartments
    Chenguang Fan
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Proc Natl Acad Sci U S A 109:14995-5000. 2012
    ..We propose that specific interactions between the termini of shell proteins and lumen enzymes have general importance for guiding the assembly and the higher level organization of bacterial MCPs...
  3. pmc The PduM protein is a structural component of the microcompartments involved in coenzyme B(12)-dependent 1,2-propanediol degradation by Salmonella enterica
    Sharmistha Sinha
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa, USA
    J Bacteriol 194:1912-8. 2012
    ..We believe that this protocol can be extended or modified for the purification of MCPs from diverse bacteria...
  4. pmc Genetic analysis of the protein shell of the microcompartments involved in coenzyme B12-dependent 1,2-propanediol degradation by Salmonella
    Shouqiang Cheng
    Iowa State University, Department of Biochemistry, Biophysics and Molecular Biology, Ames, IA 50011, USA
    J Bacteriol 193:1385-92. 2011
    ..Interestingly, a pduK deletion caused MCP aggregation, suggesting a role in the spatial organization of MCP within the cytoplasm or perhaps in segregation at cell division...
  5. pmc Short N-terminal sequences package proteins into bacterial microcompartments
    Chenguang Fan
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Proc Natl Acad Sci U S A 107:7509-14. 2010
    ..The potential uses of MCP assembly principles in nature and in biotechnology are discussed...
  6. pmc Kinetic and functional analysis of L-threonine kinase, the PduX enzyme of Salmonella enterica
    Chenguang Fan
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Biol Chem 284:20240-8. 2009
    ..The studies reported here are the first to investigate the kinetic and catalytic mechanisms of l-threonine kinase from any organism...
  7. pmc Structural insight into the mechanisms of transport across the Salmonella enterica Pdu microcompartment shell
    Christopher S Crowley
    Molecular Biology Institute, Iowa State University, Ames, Iowa 50011, USA
    J Biol Chem 285:37838-46. 2010
    ..The location of a metal redox cofactor in the pore of a shell protein suggests a novel mechanism for either transferring redox equivalents across the shell or for regenerating luminal [Fe-S] clusters...
  8. pmc Characterization of the PduS cobalamin reductase of Salmonella enterica and its role in the Pdu microcompartment
    Shouqiang Cheng
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    J Bacteriol 192:5071-80. 2010
    ..These studies further characterize PduS, an unusual MCP-associated cobalamin reductase, and, in conjunction with prior results, indicate that the Pdu MCP encapsulates a complete cobalamin assimilation system...
  9. pmc Bacterial microcompartments: their properties and paradoxes
    Shouqiang Cheng
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA
    Bioessays 30:1084-95. 2008
    ....
  10. pmc The N-terminal region of the medium subunit (PduD) packages adenosylcobalamin-dependent diol dehydratase (PduCDE) into the Pdu microcompartment
    Chenguang Fan
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Bacteriol 193:5623-8. 2011
    ..Immunoprecipitation tests indicated that the fusion proteins were encapsulated inside the MCP shell...
  11. pmc Formation of isobutene from 3-hydroxy-3-methylbutyrate by diphosphomevalonate decarboxylase
    David S Gogerty
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Appl Environ Microbiol 76:8004-10. 2010
    ..Moreover, isobutene is a gas, which might simplify its purification from a fermentation medium, substantially reducing production costs...
  12. pmc Coproduction of acetaldehyde and hydrogen during glucose fermentation by Escherichia coli
    Huilin Zhu
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Appl Environ Microbiol 77:6441-50. 2011
    ..The possibility of a more efficient "no-distill" ethanol fermentation procedure based on the coproduction of acetaldehyde and hydrogen is discussed...
  13. doi request reprint Functional characterization and mutation analysis of human ATP:Cob(I)alamin adenosyltransferase
    Chenguang Fan
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Biochemistry 47:2806-13. 2008
    ..These studies functionally defined the hATR active site and tentatively implicated three amino acid residues in facilitating the reduction of cob(II)alamin to cob(I)alamin which is a prerequisite to adenosylation...
  14. doi request reprint The PduX enzyme of Salmonella enterica is an L-threonine kinase used for coenzyme B12 synthesis
    Chenguang Fan
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Biol Chem 283:11322-9. 2008
    ..To our knowledge, PduX is the first enzyme shown to phosphorylate free L-threonine and the first L-threonine kinase shown to function in coenzyme B(12) synthesis...
  15. pmc Microcompartments for B12-dependent 1,2-propanediol degradation provide protection from DNA and cellular damage by a reactive metabolic intermediate
    Edith M Sampson
    Iowa State University, Department of Biochemistry, Biophysics and Molecular Biology, Ames, IA 50011, USA
    J Bacteriol 190:2966-71. 2008
    ..We propose that a primary function of the MCPs involved in 1,2-PD degradation is the mitigation of toxicity and DNA damage by propionaldehyde...
  16. pmc PduL is an evolutionarily distinct phosphotransacylase involved in B12-dependent 1,2-propanediol degradation by Salmonella enterica serovar typhimurium LT2
    Yu Liu
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    J Bacteriol 189:1589-96. 2007
    ..97 mM, respectively. Sequence analyses showed that PduL is unrelated in amino acid sequence to known PTAC enzymes and that PduL homologues are distributed among at least 49 bacterial species but are absent from the Archaea and Eukarya...

Research Grants5

  1. Dissecting the structure and function of the Pdu microcompartment in Salmonella
    Thomas Bobik; Fiscal Year: 2009
    ..The studies will lead to a deeper fundamental and mechanistic understanding of these important bacterial organelles, and ultimately to new opportunities for interfering with pathogenic processes. ..
  2. Conversion of Inactive Cobalamins to Coenzyme B12
    Thomas Bobik; Fiscal Year: 2005
    ..abstract_text> ..
  3. Dissecting the structure and function of the Pdu microcompartment in Salmonella
    Todd O Yeates; Fiscal Year: 2010
    ..The studies will lead to a deeper fundamental and mechanistic understanding of these important bacterial organelles, and ultimately to new opportunities for interfering with pathogenic processes. ..