M A Behlke

Summary

Affiliation: Integrated DNA Technologies
Country: USA

Publications

  1. ncbi Use of high specific activity StarFire oligonucleotide probes to visualize low-abundance pre-mRNA splicing intermediates in S. pombe
    M A Behlke
    Integrated DNA Technologies, Coralville, IA, USA
    Biotechniques 29:892-7. 2000
  2. pmc Functional polarity is introduced by Dicer processing of short substrate RNAs
    Scott D Rose
    Integrated DNA Technologies, Inc 1710 Commercial Park, Coralville, IA 52241, USA
    Nucleic Acids Res 33:4140-56. 2005
  3. pmc SNP detection in mRNA in living cells using allele specific FRET probes
    Liya Dahan
    Department of Cell Research and Immunology, Tel Aviv University, Tel Aviv, Israel Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv, Israel
    PLoS ONE 8:e72389. 2013
  4. pmc RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers
    Joseph R Dobosy
    Integrated DNA Technologies, Inc, 1710 Commercial Park, Coralville, IA 5224, USA
    BMC Biotechnol 11:80. 2011
  5. doi Chemical modification of siRNAs for in vivo use
    Mark A Behlke
    Integrated DNA Technologies, Inc, Coralville, Iowa 52241, USA
    Oligonucleotides 18:305-19. 2008
  6. ncbi Progress towards in vivo use of siRNAs
    Mark A Behlke
    Integrated DNA Technologies, Inc, Coralville, IA 52241, USA
    Mol Ther 13:644-70. 2006
  7. pmc Increased potency and longevity of gene silencing using validated Dicer substrates
    E Hefner
    Bio Rad Laboratories, Inc, Hercules, CA 94547, USA
    J Biomol Tech 19:231-7. 2008
  8. doi Chemical modification and design of anti-miRNA oligonucleotides
    K A Lennox
    Molecular Genetics and Biophysics, Integrated DNA Technologies, Coralville, IA 52241, USA
    Gene Ther 18:1111-20. 2011
  9. ncbi Central delivery of Dicer-substrate siRNA: a direct application for pain research
    Louis Doré-Savard
    Department of Physiology and Biophysics, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, Quebec, Canada
    Mol Ther 16:1331-9. 2008
  10. ncbi Respiratory syncytial virus nonstructural protein 2 specifically inhibits type I interferon signal transduction
    Murali Ramaswamy
    Department of Internal Medicine, University of Iowa Carver College of Medicine, 200 Hawkins Drive, Iowa City, IA 52242, USA
    Virology 344:328-39. 2006

Collaborators

  • Mark E Davis
  • Mohammed Amarzguioui
  • Jeremy D Heidel
  • E J Devor
  • K L Gould
  • E Hefner
  • JOHN JOSEPH ROSSI
  • Andrew S Peek
  • Richard Owczarzy
  • S Barik
  • K Clark
  • Scott D Rose
  • Michael A Collingwood
  • Andrew Tsourkas
  • Maryam Zamanian-Daryoush
  • Kim A Lennox
  • Harris S Soifer
  • Liya Dahan
  • Dong Ho Kim
  • Joseph R Dobosy
  • K A Lennox
  • Lingyan Huang
  • Joseph A Walder
  • Michael S Hildebrand
  • Antony K Chen
  • Louis Doré-Savard
  • Bryan R G Williams
  • Murali Ramaswamy
  • Yong You
  • Bernardo G Moreira
  • Marjorie A Robbins
  • Joao Trindade Marques
  • Gang Bao
  • Ranit Kedmi
  • Dan Peer
  • Kristy M Powers
  • Kristin R Beltz
  • Susan M Rupp
  • Samuel P Gubbels
  • Michael P Gantier
  • Marc André Dansereau
  • Abraham M Sheffield
  • Philippe Sarret
  • Hans Henrik M Dahl
  • Matthias John
  • Amit Kochhar
  • Chris Hillier
  • Michelle G de Silva
  • James Finke
  • Merete T Wiiger
  • Nicolas Beaudet
  • Patricia Rayman
  • Richard J H Smith
  • Stephen S Newton
  • Geneviève Roussy
  • Joao T Marques
  • Cherisa A Fletcher
  • Takashi Fujita
  • Die Wang
  • Paul Serbinowski
  • Maegan J Johnson
  • Lei Shi
  • Doris V Boyer
  • Steven M Varga
  • Thalie Devosse
  • Jaime L Sabel
  • Andrei L Laikhter
  • Dwight C Look
  • Irene Leung
  • Rune Hartmann
  • John M Dagle
  • Yong Song
  • Mingjie Li
  • Haitang Li
  • Sangdun Choi
  • Maany Peyvan
  • Mi Sook Chang
  • Adriana Zaragoza

Detail Information

Publications26

  1. ncbi Use of high specific activity StarFire oligonucleotide probes to visualize low-abundance pre-mRNA splicing intermediates in S. pombe
    M A Behlke
    Integrated DNA Technologies, Coralville, IA, USA
    Biotechniques 29:892-7. 2000
    ..Kinase-labeled tf2d probe detected only unspliced RNA, while the same oligonucleotide labeled using the new method detected both unspliced tf2d RNA and rare pre-mRNA splicing intermediates...
  2. pmc Functional polarity is introduced by Dicer processing of short substrate RNAs
    Scott D Rose
    Integrated DNA Technologies, Inc 1710 Commercial Park, Coralville, IA 52241, USA
    Nucleic Acids Res 33:4140-56. 2005
    ..Thus, Dicer processing confers functional polarity within the RNAi pathway...
  3. pmc SNP detection in mRNA in living cells using allele specific FRET probes
    Liya Dahan
    Department of Cell Research and Immunology, Tel Aviv University, Tel Aviv, Israel Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv, Israel
    PLoS ONE 8:e72389. 2013
    ..This approach promotes in vivo molecular imaging methods and could further be applied in cancer diagnosis and theranostic strategies. ..
  4. pmc RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers
    Joseph R Dobosy
    Integrated DNA Technologies, Inc, 1710 Commercial Park, Coralville, IA 5224, USA
    BMC Biotechnol 11:80. 2011
    ....
  5. doi Chemical modification of siRNAs for in vivo use
    Mark A Behlke
    Integrated DNA Technologies, Inc, Coralville, Iowa 52241, USA
    Oligonucleotides 18:305-19. 2008
    ..This review will examine studies that document the utility of various chemical modifications for use in siRNAs, both in vitro and in vivo, with close attention given to reports demonstrating actual performance in animal model systems...
  6. ncbi Progress towards in vivo use of siRNAs
    Mark A Behlke
    Integrated DNA Technologies, Inc, Coralville, IA 52241, USA
    Mol Ther 13:644-70. 2006
    ..This review will first discuss aspects of RNAi biochemistry and biology that impact in vivo use, especially as relates to experimental design, and will then provide an overview of published work with a focus on methodology...
  7. pmc Increased potency and longevity of gene silencing using validated Dicer substrates
    E Hefner
    Bio Rad Laboratories, Inc, Hercules, CA 94547, USA
    J Biomol Tech 19:231-7. 2008
    ..Furthermore, improved silencing by these 27mers is often more pronounced at lower concentrations...
  8. doi Chemical modification and design of anti-miRNA oligonucleotides
    K A Lennox
    Molecular Genetics and Biophysics, Integrated DNA Technologies, Coralville, IA 52241, USA
    Gene Ther 18:1111-20. 2011
    ..In particular, it is essential that the AMO binds with high affinity to the miRNA 'seed region', which spans bases 2-8 from the 5'-end of the miRNA...
  9. ncbi Central delivery of Dicer-substrate siRNA: a direct application for pain research
    Louis Doré-Savard
    Department of Physiology and Biophysics, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, Quebec, Canada
    Mol Ther 16:1331-9. 2008
    ..Altogether, DsiRNAi represents a potent tool for dissecting nociceptive pathways and could further lead to a new class of central active drugs...
  10. ncbi Respiratory syncytial virus nonstructural protein 2 specifically inhibits type I interferon signal transduction
    Murali Ramaswamy
    Department of Internal Medicine, University of Iowa Carver College of Medicine, 200 Hawkins Drive, Iowa City, IA 52242, USA
    Virology 344:328-39. 2006
    ..Taken together, our results indicate that NS2 regulates Stat2 levels during RSV infection, thereby modulating viral effects on interferon-dependent gene expression...
  11. pmc Hybridization of 2'-O-methyl and 2'-deoxy molecular beacons to RNA and DNA targets
    Andrew Tsourkas
    Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
    Nucleic Acids Res 30:5168-74. 2002
    ..For the pairs tested, the 2'-deoxy-beacon/DNA-target duplex showed the fastest hybridization kinetics. These findings have significant implications for the design and application of molecular beacons...
  12. ncbi Hybridization of 2'-O-methyl and 2'-deoxy molecular beacons to RNA and DNA targets
    Andrew Tsourkas
    Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
    Nucleic Acids Res 31:5168-74. 2003
    ..For the pairs tested, the 2'-deoxy-beacon/DNA-target duplex showed the fastest hybridization kinetics. These findings have significant implications for the design and application of molecular beacons...
  13. pmc Thermodynamic treatment of oligonucleotide duplex-simplex equilibria
    Richard Owczarzy
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA
    Proc Natl Acad Sci U S A 100:14840-5. 2003
    ....
  14. pmc Chemical modification patterns compatible with high potency dicer-substrate small interfering RNAs
    Michael A Collingwood
    Integrated DNA Technologies, Inc 1710 Commercial Park, Coralville, IA 52241, USA
    Oligonucleotides 18:187-200. 2008
    ....
  15. pmc Efficient cytosolic delivery of molecular beacon conjugates and flow cytometric analysis of target RNA
    Antony K Chen
    Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA
    Nucleic Acids Res 36:e69. 2008
    ..Overall, the present study introduces an improved methodology for the high-throughput detection of RNA at the single cell level...
  16. ncbi Determinants of cytokine induction by small interfering RNA in human peripheral blood mononuclear cells
    Maryam Zamanian-Daryoush
    Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Interferon Cytokine Res 28:221-33. 2008
    ..In accordance with this, specific chemical modifications differentially altered cytokine production, suggesting recruitment of different TLRs in a sequence-dependent manner...
  17. doi Advances in molecular and cellular therapies for hearing loss
    Michael S Hildebrand
    Department of Otolaryngology Head and Neck Surgery, University of Iowa, Iowa City, Iowa 52242, USA
    Mol Ther 16:224-36. 2008
    ..We explore the advantages and limitations associated with the use of these strategies for inner ear restoration...
  18. ncbi Design of active small interfering RNAs
    Andrew S Peek
    Integrated DNA Technologies Inc, 1710 Commercial Park, Coralville, IA 52241, USA
    Curr Opin Mol Ther 9:110-8. 2007
    ..In addition to rational design criteria, chemical modification of the RNA can improve function by improving stability, reducing the potential for off-target effects and avoiding stimulation of the innate immune system...
  19. ncbi Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy
    Dong Ho Kim
    Nat Biotechnol 23:222-6. 2005
    ..These results provide an alternative strategy for eliciting RNAi-mediated target cleavage using low concentrations of synthetic RNA as substrates for cellular Dicer-mediated cleavage...
  20. pmc Design of LNA probes that improve mismatch discrimination
    Yong You
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA
    Nucleic Acids Res 34:e60. 2006
    ..New guidelines are suggested for design of LNA probes, which significantly improve mismatch discrimination in comparison with unmodified DNA probes...
  21. ncbi A structural basis for discriminating between self and nonself double-stranded RNAs in mammalian cells
    Joao Trindade Marques
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Nat Biotechnol 24:559-65. 2006
    ..These findings will enable the rational design of siRNAs that avoid nonspecific effects or, alternatively, that induce bystander effects to potentially increase the efficacy of siRNA-based treatments of viral infections or cancer...
  22. ncbi Stable expression of shRNAs in human CD34+ progenitor cells can avoid induction of interferon responses to siRNAs in vitro
    Marjorie A Robbins
    Division of Molecular Biology, Beckman Research Institute, City of Hope, Duarte, California 91010, USA
    Nat Biotechnol 24:566-71. 2006
    ..We show that in this system, lipid-delivered siRNAs are potent inducers of IFNalpha and type I IFN gene expression, whereas the same sequences when expressed endogenously are nonimmunostimulatory...
  23. ncbi Characterization of modified antisense oligonucleotides in Xenopus laevis embryos
    Kim A Lennox
    Integrated DNA Technologies, Coralville, IA 52241, USA
    Oligonucleotides 16:26-42. 2006
    ..However, at 400 nM, both of these compounds led to significant degradation of survivin mRNA, even when up to three mismatches were present in the heteroduplex...
  24. pmc A potential role for RNA interference in controlling the activity of the human LINE-1 retrotransposon
    Harris S Soifer
    Division of Molecular Biology, Beckman Research Institute of the City of Hope 1450 East Duarte Road, Duarte, CA 91010 3011, USA
    Nucleic Acids Res 33:846-56. 2005
    ..Finally, both synthetic and pooled siRNA suppressed retrotransposition from a highly active RC-L1 clone in cell culture assay. Our report is the first to demonstrate that a human transposable element is subjected to RNAi...
  25. ncbi Rational design and in vitro and in vivo delivery of Dicer substrate siRNA
    Mohammed Amarzguioui
    The Biotechnology Centre of Oslo, Gaustadalleen 21, 0349 Oslo, Norway
    Nat Protoc 1:508-17. 2006
    ..siRNA delivery and transfection and analysis of macrophages in vivo can be accomplished within 36 h...