Research Topics
Genomes and Genes
| Edward A MiaoSummaryAffiliation: Institute for Systems Biology Country: USA Publications
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Detail Information
Publications
Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteriaEdward A Miao
Institute for Systems Biology, Seattle, Washington, USA
Nat Immunol 11:1136-42. 2010..This demonstrates that activation of caspase-1 clears intracellular bacteria in vivo independently of IL-1β and IL-18 and establishes pyroptosis as an efficient mechanism of bacterial clearance by the innate immune system...- Innate immune detection of bacterial virulence factors via the NLRC4 inflammasomeEdward A Miao
Institute for Systems Biology, 1441 N 34th St, Seattle, WA 98103, USA
J Clin Immunol 30:502-6. 2010..Both flagellin and rod protein are unintentionally delivered to the mammalian cytosol by the bacterium through the T3SS... - Innate immune detection of the type III secretion apparatus through the NLRC4 inflammasomeEdward A Miao
Institute for Systems Biology, Seattle, WA 98103, USA
Proc Natl Acad Sci U S A 107:3076-80. 2010..Furthermore, the specific detection of the virulence machinery permits the discrimination between pathogenic and nonpathogenic bacteria... - Pseudomonas aeruginosa activates caspase 1 through IpafEdward A Miao
Institute for Systems Biology, Seattle, WA 98103, USA
Proc Natl Acad Sci U S A 105:2562-7. 2008..Thus, Ipaf-dependent detection of cytosolic flagellin is a conserved mechanism by which macrophages detect the presence of pathogens that use T3SS... - Cutting edge: Cytosolic bacterial DNA activates the inflammasome via Aim2Sarah E Warren
Institute for Systems Biology, Seattle, WA 98103, USA
J Immunol 185:818-21. 2010..We now use biochemical and genetic approaches to demonstrate that the third detector senses bacterial DNA and identify it as Aim2, a receptor that has previously been shown to detect viral DNA... - Generation of a Listeria vaccine strain by enhanced caspase-1 activationSarah E Warren
Institute for Systems Biology, Seattle, WA 98109, USA
Eur J Immunol 41:1934-40. 2011..monocytogenes-based vaccine. Moreover, the attenuated strains with embedded flagellin or PrgJ adjuvants represent attractive vectors for vaccines aimed at eliciting T-cell responses... 
Cytoplasmic flagellin activates caspase-1 and secretion of interleukin 1beta via IpafEdward A Miao
Institute for Systems Biology, Seattle, Washington 98103, USA
Nat Immunol 7:569-75. 2006....- Virus binding to a plasma membrane receptor triggers interleukin-1 alpha-mediated proinflammatory macrophage response in vivoNelson C Di Paolo
Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA 98195, USA
Immunity 31:110-21. 2009..Thus, these data identify IL-1 alpha-IL-1RI as a key pathway allowing for the activation of proinflammatory responses to the virus, independently of its genomic nucleic acid recognition... - Activation of the NLRP3 inflammasome by intracellular poly I:CJayant V Rajan
Institute for Systems Biology, Seattle, WA 98103, USA
FEBS Lett 584:4627-32. 2010..This detection is independent of the other poly I:C sensors: TLR3 and MDA5. These results suggest a mechanism by which cytosolic dsRNA produced during viral infection could activate the NLRP3 inflammasome... - The NLRP3 inflammasome detects encephalomyocarditis virus and vesicular stomatitis virus infectionJayant V Rajan
Institute for Systems Biology, 1441 34th Avenue N, Seattle, WA 98104, USA
J Virol 85:4167-72. 2011..These findings indicate that the NLRP3 inflammasome may be a common pathway for RNA virus detection, but its precise role in the host response may be variable... - TLR5 and Ipaf: dual sensors of bacterial flagellin in the innate immune systemEdward A Miao
Institute for Systems Biology, Seattle, WA 98103, USA
Semin Immunopathol 29:275-88. 2007..Thus, TLR5 responds more generally to flagellated bacteria, while Ipaf responds to bacteria that express both flagellin and virulence factors... - Salmonella effectors translocated across the vacuolar membrane interact with the actin cytoskeletonEdward A Miao
Department of Microbiology, University of Washington, HSB K-140, Box 357710, Seattle, WA 98195-7710, USA
Mol Microbiol 48:401-15. 2003..However, a third intracellular virulence factor, spvB, which ADP-ribosylates actin, strongly inhibited VAP formation in HeLa cells, suggesting a more subtle effect for SspH2 and SseI on the actin cytoskeleton... - Transcription of the SsrAB regulon is repressed by alkaline pH and is independent of PhoPQ and magnesium concentrationEdward A Miao
Department of Microbiology, Health Sciences Building, University of Washington, Seattle, WA 98195, USA
J Bacteriol 184:1493-7. 2002..0 or at a lower pH, suggesting that alkaline pH represses SsrAB activation. In addition, the PhoPQ two-component system, which is also activated intracellularly, was not required for activation of SsrAB...