Stephen R Lasky

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..Together, our data support a model in which Nup2p movement facilitates the transition between the import and export phases of nucleocytoplasmic transport...

..We have entered the cell, the Mansion of our birth and started the inventory of our acquired wealth." (Albert Claude, Nobel lecture, 1974)...

..This review focuses on the biogenesis of peroxisomes with an emphasis on the regulation of peroxisome formation and the import of peroxisomal matrix proteins in the model organism Saccharomyces cerevisiae...

..Rho1p regulates the assembly state of actin on the peroxisome membrane, thereby controlling peroxisome membrane dynamics and biogenesis...

..By integration with extant data sets, we present a total of 211 genes linked to peroxisome biogenesis and highlight the complex networks through which information flows during peroxisome biogenesis and function...

..A benchtop inkjet arrayer-nicknamed POSAM-can be built by most skilled molecular biology laboratories. Inkjet arrays can fulfill the changing needs of those studying the complex network of relationships in systems biology...

..We describe here an open-source inkjet arrayer capable of rapidly producing sets of unique 9,800-feature arrays...

..Our study represents the first global study of signaling networks regulating the biogenesis of an organelle...

..The Institute for Systems Biology has aspired to pioneer and seamlessly integrate each of these concepts...

..The OFFNM is highly overrepresented in the yeast regulome, suggesting that the specific functions described for the OFFNM, or other properties of this motif, provide a selective advantage...

..These studies demonstrate the utility of comparative gene profiling as an alternative to functional assays to identify genes with roles in peroxisome biogenesis...

..The methods for the analyses of the yeast peroxisome are described in detail; however, these concepts are generally applicable to the study of other organelles...

..Notably, our method is theoretically applicable to any type of modification or chemical moiety generating a unique fragment ion pattern...

..While these new nucleosomes do not incorporate Htz1p, the initial presence of Htz1p appears to mark the promoter for sustained gene expression and the recruitment of TATA-binding protein...

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..doi:10.1083/jcb.200909013), have combined systems genetics with high-throughput and high-content imaging to comprehensively identify and classify novel components that contribute to the morphology and function of the mitotic spindle...

..This method has general utility for multiple applications where isotopic labeling is used for quantification in mass spectrometry...

..For each combination of these categories, a model is presented and discussed in the context of the yeast cell cycle, illustrating how different types of questions can be addressed by different model classes...

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..Together, these data suggest a network mechanism involving dynamic combinatorial control for coordinating transcriptional responses...

..Together these data demonstrate that Ste12p is imported into nuclei by Kap121p and that mating and differentiation defects associated with kap121 mutants are primarily attributable to the mislocalization of Ste12p...

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..Together, our data elucidate additional levels of complexity in these nuclear transport pathways...

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..We show that these complexes are important for the faithful maintenance of an established boundary, as disruption of the complexes results in specific, anomalous alterations of the silent and active epigenetic states...

..Our method overcomes many of the previous limitations to produce large RNP interactomes with almost no contamination...

..Understanding peroxisome dynamics, biogenesis and function are critical to understanding these numerous human conditions and to their future treatment. ..

..Preliminary results applying these techniques demonstrate that the first these proteins is a previously unknown peroxin required for peroxisome size regulation and segregation during cell division. ..

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..Understanding peroxisome dynamics, biogenesis and function are critical to understanding these numerous human conditions and to their future treatment. ..