Research Topics
Species | F A WitzmannSummaryAffiliation: Indiana University Country: USA Publications
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Publications
Cutting-edge technology. II. Proteomics: core technologies and applications in physiologyFrank A Witzmann
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
Am J Physiol Gastrointest Liver Physiol 282:G735-41. 2002..In this review, we take a brief and concise look at some of the current, physiologically relevant technologies that comprise proteomics and report specific applications in which proteomics has provided valuable biological insight...- Proteomic analysis of the renal effects of simulated occupational jet fuel exposureF A Witzmann
Department of Biology, Indiana University Purdue University, Columbus 47203, USA
Electrophoresis 21:976-84. 2000..Human risk is suggested by these data but conclusive assessment awaits a noninvasive search for biomarkers in JP-8 exposed humans... - A proteomic survey of rat cerebral cortical synaptosomesFrank A Witzmann
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, 46202, USA
Proteomics 5:2177-201. 2005..The results also suggest that this experimental approach can be used to study the differential expression of multiple proteins involved in alterations of synaptic function... - Effect of JP-8 jet fuel exposure on protein expression in human keratinocyte cells in cultureF A Witzmann
Department of Cellular and Integrative Physiology, Biotechnology Research and Training Center, Indiana University School of Medicine, 1345 W 16th Street, Rm 308, Indianapolis, IN 46202 2111, USA
Toxicol Lett 160:8-21. 2005..In general, altered proteins involved endocytotic/exocytotic mechanisms and their cytoskeletal components, cell stress, and those involved in vesicular function... - Two-dimensional gels for toxicological drug discovery applicationsFrank A Witzmann
Indiana University School of Medicine, Department of Cellular and Integrative Physiology, Biotechnology and Research Training Center, Indianapolis, IN 46202, USA
Expert Opin Drug Metab Toxicol 2:103-11. 2006.... - Innate differences in protein expression in the nucleus accumbens and hippocampus of inbred alcohol-preferring and -nonpreferring ratsFrank A Witzmann
Department of Cellular and Integrative Physiology, Biotechnology Research and Training Center, Indiana University School of Medicine, 1345 W 16th Street, Indianapolis, IN 46202, USA
Proteomics 3:1335-44. 2003.... - Analysis of rat testicular protein expression following 91-day exposure to JP-8 jet fuel vaporFrank A Witzmann
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
Proteomics 3:1016-27. 2003.... - Proteomics and nephrotoxicityFrank A Witzmann
Departments of Cellular and Integrative Physiology and Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Ind, USA
Contrib Nephrol 141:104-23. 2004 - Multi-walled carbon nanotube exposure alters protein expression in human keratinocytesFrank A Witzmann
Department of Cellular and Integrative Physiology, Biotechnology Research and Training Center, Indiana University School of Medicine, Indianapolis, Indiana, USA
Nanomedicine 2:158-68. 2006..These data clearly show that MWCNTs are capable of altering protein expression in a target epithelial cell that constitutes a primary route of occupational exposure for manufactured nanotubes... - Toxicity of chemical mixtures: proteomic analysis of persisting liver and kidney protein alterations induced by repeated exposure of rats to JP-8 jet fuel vaporF A Witzmann
Department of Biology, Indiana University Purdue University, Columbus 47204, USA
Electrophoresis 21:2138-47. 2000..001) in expressed lamin A and 10-FTHF DH. These persisting changes in liver and kidney proteins are discussed in terms of possible alterations in the functional capacity of exposed subjects... - Gels and more gels: probing toxicityFrank A Witzmann
Indiana University School of Medicine, Department of Cellular and Integrative Physiology, Biotechnology and Research Training Center, 1345 West 16th Street, Indianapolis, IN 46202, USA
Curr Opin Mol Ther 6:608-15. 2004.... - Proteomic analysis of simulated occupational jet fuel exposure in the lungF A Witzmann
Department of Biology, Indiana University Purdue University, Columbus 47203, USA
Electrophoresis 20:3659-69. 1999..e., mitochondria, microsomes, and nuclei of lung and other targets... - Differential expression of cytosolic proteins in the rat kidney cortex and medulla: preliminary proteomicsF A Witzmann
Department of Biology, Indiana University Purdue University, Columbus 47203, USA
Electrophoresis 19:2491-7. 1998..These results both confirm and expand our understanding of the molecular heterogeneity characterizing structurally and functionally distinct regions of the kidney and serve as a useful foundation for future nephrotoxicologic studies... - Glutathione S-transferases: two-dimensional electrophoretic protein markers of lead exposureF A Witzmann
Department of Biology, Indiana University Purdue University, Columbus 47203, USA
Electrophoresis 19:1332-5. 1998..These preliminary data confirm the utility of 2-D electrophoretic GST analysis as indicative of lead exposure and toxicity and support its use for further elaboration of lead's effects on renal protein expression... - Regional protein alterations in rat kidneys induced by lead exposureF A Witzmann
Department of Biology, Indiana University Purdue University, Columbus 47203, USA
Electrophoresis 20:943-51. 1999..These observations reflect the complexity of lead's nephrotoxicity and endorse the application of proteomics in mechanistic studies as well as biomarker development in a variety of toxicologic paradigms... - Protein expression changes in the nucleus accumbens and amygdala of inbred alcohol-preferring rats given either continuous or scheduled access to ethanolR L Bell
Institute of Psychiatric Research and Department of Psychiatry, Indiana University School of Medicine, Indianapolis, IN 46202, USA
Alcohol 40:3-17. 2006..This may reflect differences in neuroanatomical and/or functional characteristics associated with EtOH self-administration and possibly withdrawal, between these two brain structures... - Transducin subunit stoichiometry and cellular distribution in rod outer segmentsJames W Clack
Department of Biology, Indiana University, Purdue University at Indianapolis, 4601 Central Ave, Columbus, IN 47203, USA
Cell Biol Int 30:829-35. 2006..These results suggest that the production and/or turnover of G(alphat), G(beta), and G(gamma) in the rod outer segment are controlled independently... - Pharmacoproteomics in drug developmentFrank A Witzmann
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, 1345 W 16th Street, Indianapolis, IN 46202, USA
Pharmacogenomics J 3:69-76. 2003.... - Proteomic analysis of transducin beta-subunit structural heterogeneityJames W Clack
Department of Biology, Indiana University Purdue University at Indianapolis, Columbus 47203, USA
Electrophoresis 24:3493-9. 2003..We also conclude that some, but not all, of the heterogeneity observed is due to phosphorylation of Tb1... - Venous and arterial endothelial proteomics: mining for markers and mechanisms of endothelial diversityMatthew R Richardson
Herman B Wells Center for Pediatric Research, Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN 46202, USA
Expert Rev Proteomics 7:823-31. 2010..It is the purpose of this article to account for the diversity of vascular EC proteomic investigations and comment on the issues that have been and should be addressed in future work... - Toxicant-induced alterations in two-dimensional electrophoretic patterns of hepatic and renal stress proteinsF A Witzmann
Molecular Anatomy Laboratory, Indiana University Purdue University, Columbus, USA
Electrophoresis 17:198-202. 1996..Furthermore, because nearly all of the proteins studied were constitutively expressed at detectable levels in both liver and kidney, the 2-DE stress protein pattern may be suitable to future toxicologic screening applications... - Proteomics and alcoholismFrank A Witzmann
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
Int Rev Neurobiol 61:189-214. 2004 - Proteomic analysis of differential protein expression induced by ultraviolet light radiation in HeLa cellsEmily D Decker
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, 46202, USA
Proteomics 3:2019-27. 2003..This proteomic approach provides opportunities to gain insights into the mechanism by which DNA replication is inhibited... - Impaired collateral development in mature ratsJay L Tuttle
Department of Surgery, Indiana University School of Medicine, Indianapolis, 46202, USA
Am J Physiol Heart Circ Physiol 283:H146-55. 2002..Alteration in arterial protein expression with maturation and altered responses to stimuli for collateral development may contribute to this impairment... - A proteomic workflow for discovery of serum carrier protein-bound biomarker candidates of alcohol abuse using LC-MS/MSXianyin Lai
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
Electrophoresis 30:2207-14. 2009..Altered abundance of these proteins suggests that they may be potential novel biomarkers for alcohol abuse... - Tumor Necrosis Factor-alpha- and interleukin-1-induced cellular responses: coupling proteomic and genomic informationLee W Ott
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, MS 4053, Indianapolis, Indiana 46202, USA
J Proteome Res 6:2176-85. 2007..When combined with genomic studies, our results indicate that TNFalpha, but not IL-1, mediates cell cycle arrest... - A pilot study of proteomic profiles of human hepatocellular carcinoma in the United StatesJesus M Matos
Department of Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA
J Surg Res 155:237-43. 2009..Validation and further investigation of these protein changes may lead to the discovery of new molecular targets for therapy, biomarkers for early detection, and new endpoints for therapeutic efficacy and toxicity... - A software utility for creating interactive maps for 2D gel-based proteomicsJunyu Li
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN, USA
Anal Biochem 332:187-90. 2004 - Proteomic evaluation of cell preparation methods in primary hepatocyte cell cultureFrank A Witzmann
Departments of Cellular and Integrative Physiology and Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
Electrophoresis 23:2223-32. 2002..These results emphasize the variation introduced by cell-handling during recovery of hepatocytes from culture plates and may explain at least some of the artifactual differences observed in earlier in vitro experiments... - Diabetic dyslipidemia and exercise alter the plasma low-density lipoproteome in Yucatan pigsMatthew R Richardson
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
Proteomics 9:2468-83. 2009..Taken together, this work identifies new possible biomarkers, potential therapeutic targets for atherosclerosis, and generates new hypotheses regarding the role of LDL in atherogenesis... - Sample complexity reduction for two-dimensional electrophoresis using solution isoelectric focusing prefractionationMatthew R Richardson
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN, USA
Electrophoresis 29:2637-44. 2008..5-fold increase over the 1577 spots detected in an unfractionated gel. We concluded that sIEF is an effective means of prefractionation to increase depth of field and improve the analysis of low-abundance proteins... - Protein composition of liver cyst fluid from the BALB/c-cpk/+ mouse model of autosomal recessive polycystic kidney diseaseXianyin Lai
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
Proteomics 9:3775-82. 2009..Protein identifications have been submitted to the PRIDE database (http://www.ebi.ac.uk/pride), accession number 9227... - Application of pressure cycling technology to tissue sample preparation for 2-DEHeather Ringham
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202 2111, USA
Electrophoresis 28:1022-4. 2007..In an attempt to improve the yield of detectable proteins during sample preparation, we applied a novel extraction technique called pressure cycling technology... - Variability of protein content in calcium oxalate monohydrate stonesJames C Williams
Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202 5120, USA
J Endourol 20:560-4. 2006.... - Serine/threonine/tyrosine phosphorylation of the LHX3 LIM-homeodomain transcription factorGretchen E Parker
Department of Biology, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202 5132, USA
J Cell Biochem 94:67-80. 2005..In conclusion, we have identified unique amino acids within LHX3 that are important for its transcriptional activity and are phosphorylated... - The impact of blood contamination on the proteome of cerebrospinal fluidJin-Sam You
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, USA
Proteomics 5:290-6. 2005..This analysis was aimed at identification of proteins found primarily in CSF, those found primarily in blood and assessment of the impact of blood contamination on those proteins found in both fluids... - JP-8 jet fuel exposure alters protein expression in the lungMatthew G Drake
Joan B and Donald R Diamond Lung Injury Laboratory, Department of Pediatrics, Arizona Health Sciences Center, 1501 N Campbell Avenue, Room 3354, Tucson, AZ 85724, USA
Toxicology 191:199-210. 2003..Furthermore, there was a decrease in alpha1-anti-trypsin expression suggesting that JP-8 jet fuel exposure may have implications for the development of pulmonary disorders... - Synaptosome proteomicsFengju Bai
Safety Sciences, Charles River Laboratories Preclinical Services, Worcester, Massachusetts, USA
Subcell Biochem 43:77-98. 2007.... - Proteomic and inducible transgenic approaches to study disease processesPrabir Ray
Dorothy P and Richard P Simmons Center for Research and Education in Interstitial Lung Disease, Division of Pulmonary, Allergy, and Critical Care Medicine and Department of Immunology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
Am J Respir Cell Mol Biol 29:S42-6. 2003 - On the dependence of a characterization of proteomics maps on the number of protein spots consideredMilan Randić
National Institute of Chemistry, Ljubljana, Slovenia
J Chem Inf Model 46:116-22. 2006..We found that proteins maps based on a set of about the 250 most abundant proteins spots suffice for a satisfactory numerical characterization of such maps... - Mitochondrial matrix phosphoproteome: effect of extra mitochondrial calciumRachel K Hopper
Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892-1061, USA
Biochemistry 45:2524-36. 2006..These data demonstrate the use of a phosphoproteome screen in determining mitochondrial signaling pathways and reveal new pathways for Ca(2+) modification of mitochondrial function at the level of MnSOD... - Proteomics of S-(1, 2-dichlorovinyl)-L-cysteine-induced acute renal failure and autoprotection in miceMidhun C Korrapati
Department of Toxicology, College of Pharmacy, University of Louisiana Monroe, Monroe, Louisiana 71209 0470, USA
Am J Physiol Renal Physiol 293:F994-F1006. 2007..This study provides the first report on proteomics of DCVC-induced ARF and autoprotection in mice and reflects the application of proteomics in mechanistic studies as well as biomarker development in a variety of toxicological paradigms... - Sample preparation for two-dimensional gel electrophoresis using pressure cycling technologyGary B Smejkal
Proteomics and Small Molecules Applications Laboratory, Pressure BioSciences, Woburn, MA 01801, USA
Anal Biochem 363:309-11. 2007 - Proteomics: technologies and applicationsMark P Molloy
Pfizer Global Research and Development, Ann Arbor Laboratories, USA
Brief Funct Genomic Proteomic 1:23-39. 2002..Some areas where proteomics has delivered valuable biological insight will also be considered, and there will be a look towards future areas of development...
Research Grants
- Synaptosomal Protein Changes After EtOH Self-AdministrationFrank Witzmann; Fiscal Year: 2006....
- Protein Biomarker Profiles of Abusive Alcohol ConsumptionFrank Witzmann; Fiscal Year: 2007....
- Nanoparticle Effects on Epithelial Cell Protein Expression and FunctionFrank A Witzmann; Fiscal Year: 2010..In addition, the studies will correlate the changes in protein expression with changes in cellular function at both toxic and subtoxic levels. ..
- Nanoparticle Effects on Epithelial Cell Protein Expression and FunctionFrank Witzmann; Fiscal Year: 2009..In addition, the studies will correlate the changes in protein expression with changes in cellular function at both toxic and subtoxic levels. ..