F A Witzmann

Summary

Affiliation: Indiana University
Country: USA

Publications

  1. ncbi request reprint Cutting-edge technology. II. Proteomics: core technologies and applications in physiology
    Frank A Witzmann
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    Am J Physiol Gastrointest Liver Physiol 282:G735-41. 2002
  2. ncbi request reprint Proteomic analysis of the renal effects of simulated occupational jet fuel exposure
    F A Witzmann
    Department of Biology, Indiana University Purdue University, Columbus 47203, USA
    Electrophoresis 21:976-84. 2000
  3. pmc A proteomic survey of rat cerebral cortical synaptosomes
    Frank A Witzmann
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, 46202, USA
    Proteomics 5:2177-201. 2005
  4. ncbi request reprint Effect of JP-8 jet fuel exposure on protein expression in human keratinocyte cells in culture
    F A Witzmann
    Department of Cellular and Integrative Physiology, Biotechnology Research and Training Center, Indiana University School of Medicine, 1345 W 16th Street, Rm 308, Indianapolis, IN 46202 2111, USA
    Toxicol Lett 160:8-21. 2005
  5. ncbi request reprint Two-dimensional gels for toxicological drug discovery applications
    Frank A Witzmann
    Indiana University School of Medicine, Department of Cellular and Integrative Physiology, Biotechnology and Research Training Center, Indianapolis, IN 46202, USA
    Expert Opin Drug Metab Toxicol 2:103-11. 2006
  6. pmc Innate differences in protein expression in the nucleus accumbens and hippocampus of inbred alcohol-preferring and -nonpreferring rats
    Frank A Witzmann
    Department of Cellular and Integrative Physiology, Biotechnology Research and Training Center, Indiana University School of Medicine, 1345 W 16th Street, Indianapolis, IN 46202, USA
    Proteomics 3:1335-44. 2003
  7. ncbi request reprint Analysis of rat testicular protein expression following 91-day exposure to JP-8 jet fuel vapor
    Frank A Witzmann
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Proteomics 3:1016-27. 2003
  8. ncbi request reprint Proteomics and nephrotoxicity
    Frank A Witzmann
    Departments of Cellular and Integrative Physiology and Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Ind, USA
    Contrib Nephrol 141:104-23. 2004
  9. ncbi request reprint Multi-walled carbon nanotube exposure alters protein expression in human keratinocytes
    Frank A Witzmann
    Department of Cellular and Integrative Physiology, Biotechnology Research and Training Center, Indiana University School of Medicine, Indianapolis, Indiana, USA
    Nanomedicine 2:158-68. 2006
  10. ncbi request reprint Toxicity of chemical mixtures: proteomic analysis of persisting liver and kidney protein alterations induced by repeated exposure of rats to JP-8 jet fuel vapor
    F A Witzmann
    Department of Biology, Indiana University Purdue University, Columbus 47204, USA
    Electrophoresis 21:2138-47. 2000

Collaborators

Detail Information

Publications44

  1. ncbi request reprint Cutting-edge technology. II. Proteomics: core technologies and applications in physiology
    Frank A Witzmann
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    Am J Physiol Gastrointest Liver Physiol 282:G735-41. 2002
    ..In this review, we take a brief and concise look at some of the current, physiologically relevant technologies that comprise proteomics and report specific applications in which proteomics has provided valuable biological insight...
  2. ncbi request reprint Proteomic analysis of the renal effects of simulated occupational jet fuel exposure
    F A Witzmann
    Department of Biology, Indiana University Purdue University, Columbus 47203, USA
    Electrophoresis 21:976-84. 2000
    ..Human risk is suggested by these data but conclusive assessment awaits a noninvasive search for biomarkers in JP-8 exposed humans...
  3. pmc A proteomic survey of rat cerebral cortical synaptosomes
    Frank A Witzmann
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, 46202, USA
    Proteomics 5:2177-201. 2005
    ..The results also suggest that this experimental approach can be used to study the differential expression of multiple proteins involved in alterations of synaptic function...
  4. ncbi request reprint Effect of JP-8 jet fuel exposure on protein expression in human keratinocyte cells in culture
    F A Witzmann
    Department of Cellular and Integrative Physiology, Biotechnology Research and Training Center, Indiana University School of Medicine, 1345 W 16th Street, Rm 308, Indianapolis, IN 46202 2111, USA
    Toxicol Lett 160:8-21. 2005
    ..In general, altered proteins involved endocytotic/exocytotic mechanisms and their cytoskeletal components, cell stress, and those involved in vesicular function...
  5. ncbi request reprint Two-dimensional gels for toxicological drug discovery applications
    Frank A Witzmann
    Indiana University School of Medicine, Department of Cellular and Integrative Physiology, Biotechnology and Research Training Center, Indianapolis, IN 46202, USA
    Expert Opin Drug Metab Toxicol 2:103-11. 2006
    ....
  6. pmc Innate differences in protein expression in the nucleus accumbens and hippocampus of inbred alcohol-preferring and -nonpreferring rats
    Frank A Witzmann
    Department of Cellular and Integrative Physiology, Biotechnology Research and Training Center, Indiana University School of Medicine, 1345 W 16th Street, Indianapolis, IN 46202, USA
    Proteomics 3:1335-44. 2003
    ....
  7. ncbi request reprint Analysis of rat testicular protein expression following 91-day exposure to JP-8 jet fuel vapor
    Frank A Witzmann
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Proteomics 3:1016-27. 2003
    ....
  8. ncbi request reprint Proteomics and nephrotoxicity
    Frank A Witzmann
    Departments of Cellular and Integrative Physiology and Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Ind, USA
    Contrib Nephrol 141:104-23. 2004
  9. ncbi request reprint Multi-walled carbon nanotube exposure alters protein expression in human keratinocytes
    Frank A Witzmann
    Department of Cellular and Integrative Physiology, Biotechnology Research and Training Center, Indiana University School of Medicine, Indianapolis, Indiana, USA
    Nanomedicine 2:158-68. 2006
    ..These data clearly show that MWCNTs are capable of altering protein expression in a target epithelial cell that constitutes a primary route of occupational exposure for manufactured nanotubes...
  10. ncbi request reprint Toxicity of chemical mixtures: proteomic analysis of persisting liver and kidney protein alterations induced by repeated exposure of rats to JP-8 jet fuel vapor
    F A Witzmann
    Department of Biology, Indiana University Purdue University, Columbus 47204, USA
    Electrophoresis 21:2138-47. 2000
    ..001) in expressed lamin A and 10-FTHF DH. These persisting changes in liver and kidney proteins are discussed in terms of possible alterations in the functional capacity of exposed subjects...
  11. ncbi request reprint Gels and more gels: probing toxicity
    Frank A Witzmann
    Indiana University School of Medicine, Department of Cellular and Integrative Physiology, Biotechnology and Research Training Center, 1345 West 16th Street, Indianapolis, IN 46202, USA
    Curr Opin Mol Ther 6:608-15. 2004
    ....
  12. ncbi request reprint Proteomic analysis of simulated occupational jet fuel exposure in the lung
    F A Witzmann
    Department of Biology, Indiana University Purdue University, Columbus 47203, USA
    Electrophoresis 20:3659-69. 1999
    ..e., mitochondria, microsomes, and nuclei of lung and other targets...
  13. ncbi request reprint Differential expression of cytosolic proteins in the rat kidney cortex and medulla: preliminary proteomics
    F A Witzmann
    Department of Biology, Indiana University Purdue University, Columbus 47203, USA
    Electrophoresis 19:2491-7. 1998
    ..These results both confirm and expand our understanding of the molecular heterogeneity characterizing structurally and functionally distinct regions of the kidney and serve as a useful foundation for future nephrotoxicologic studies...
  14. ncbi request reprint Glutathione S-transferases: two-dimensional electrophoretic protein markers of lead exposure
    F A Witzmann
    Department of Biology, Indiana University Purdue University, Columbus 47203, USA
    Electrophoresis 19:1332-5. 1998
    ..These preliminary data confirm the utility of 2-D electrophoretic GST analysis as indicative of lead exposure and toxicity and support its use for further elaboration of lead's effects on renal protein expression...
  15. ncbi request reprint Regional protein alterations in rat kidneys induced by lead exposure
    F A Witzmann
    Department of Biology, Indiana University Purdue University, Columbus 47203, USA
    Electrophoresis 20:943-51. 1999
    ..These observations reflect the complexity of lead's nephrotoxicity and endorse the application of proteomics in mechanistic studies as well as biomarker development in a variety of toxicologic paradigms...
  16. ncbi request reprint Protein expression changes in the nucleus accumbens and amygdala of inbred alcohol-preferring rats given either continuous or scheduled access to ethanol
    R L Bell
    Institute of Psychiatric Research and Department of Psychiatry, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Alcohol 40:3-17. 2006
    ..This may reflect differences in neuroanatomical and/or functional characteristics associated with EtOH self-administration and possibly withdrawal, between these two brain structures...
  17. ncbi request reprint Transducin subunit stoichiometry and cellular distribution in rod outer segments
    James W Clack
    Department of Biology, Indiana University, Purdue University at Indianapolis, 4601 Central Ave, Columbus, IN 47203, USA
    Cell Biol Int 30:829-35. 2006
    ..These results suggest that the production and/or turnover of G(alphat), G(beta), and G(gamma) in the rod outer segment are controlled independently...
  18. ncbi request reprint Pharmacoproteomics in drug development
    Frank A Witzmann
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, 1345 W 16th Street, Indianapolis, IN 46202, USA
    Pharmacogenomics J 3:69-76. 2003
    ....
  19. ncbi request reprint Proteomic analysis of transducin beta-subunit structural heterogeneity
    James W Clack
    Department of Biology, Indiana University Purdue University at Indianapolis, Columbus 47203, USA
    Electrophoresis 24:3493-9. 2003
    ..We also conclude that some, but not all, of the heterogeneity observed is due to phosphorylation of Tb1...
  20. pmc Venous and arterial endothelial proteomics: mining for markers and mechanisms of endothelial diversity
    Matthew R Richardson
    Herman B Wells Center for Pediatric Research, Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Expert Rev Proteomics 7:823-31. 2010
    ..It is the purpose of this article to account for the diversity of vascular EC proteomic investigations and comment on the issues that have been and should be addressed in future work...
  21. ncbi request reprint Toxicant-induced alterations in two-dimensional electrophoretic patterns of hepatic and renal stress proteins
    F A Witzmann
    Molecular Anatomy Laboratory, Indiana University Purdue University, Columbus, USA
    Electrophoresis 17:198-202. 1996
    ..Furthermore, because nearly all of the proteins studied were constitutively expressed at detectable levels in both liver and kidney, the 2-DE stress protein pattern may be suitable to future toxicologic screening applications...
  22. ncbi request reprint Proteomics and alcoholism
    Frank A Witzmann
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Int Rev Neurobiol 61:189-214. 2004
  23. ncbi request reprint Proteomic analysis of differential protein expression induced by ultraviolet light radiation in HeLa cells
    Emily D Decker
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, 46202, USA
    Proteomics 3:2019-27. 2003
    ..This proteomic approach provides opportunities to gain insights into the mechanism by which DNA replication is inhibited...
  24. ncbi request reprint Impaired collateral development in mature rats
    Jay L Tuttle
    Department of Surgery, Indiana University School of Medicine, Indianapolis, 46202, USA
    Am J Physiol Heart Circ Physiol 283:H146-55. 2002
    ..Alteration in arterial protein expression with maturation and altered responses to stimuli for collateral development may contribute to this impairment...
  25. pmc A proteomic workflow for discovery of serum carrier protein-bound biomarker candidates of alcohol abuse using LC-MS/MS
    Xianyin Lai
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Electrophoresis 30:2207-14. 2009
    ..Altered abundance of these proteins suggests that they may be potential novel biomarkers for alcohol abuse...
  26. pmc Tumor Necrosis Factor-alpha- and interleukin-1-induced cellular responses: coupling proteomic and genomic information
    Lee W Ott
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, MS 4053, Indianapolis, Indiana 46202, USA
    J Proteome Res 6:2176-85. 2007
    ..When combined with genomic studies, our results indicate that TNFalpha, but not IL-1, mediates cell cycle arrest...
  27. pmc A pilot study of proteomic profiles of human hepatocellular carcinoma in the United States
    Jesus M Matos
    Department of Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA
    J Surg Res 155:237-43. 2009
    ..Validation and further investigation of these protein changes may lead to the discovery of new molecular targets for therapy, biomarkers for early detection, and new endpoints for therapeutic efficacy and toxicity...
  28. ncbi request reprint A software utility for creating interactive maps for 2D gel-based proteomics
    Junyu Li
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN, USA
    Anal Biochem 332:187-90. 2004
  29. ncbi request reprint Proteomic evaluation of cell preparation methods in primary hepatocyte cell culture
    Frank A Witzmann
    Departments of Cellular and Integrative Physiology and Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Electrophoresis 23:2223-32. 2002
    ..These results emphasize the variation introduced by cell-handling during recovery of hepatocytes from culture plates and may explain at least some of the artifactual differences observed in earlier in vitro experiments...
  30. pmc Diabetic dyslipidemia and exercise alter the plasma low-density lipoproteome in Yucatan pigs
    Matthew R Richardson
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Proteomics 9:2468-83. 2009
    ..Taken together, this work identifies new possible biomarkers, potential therapeutic targets for atherosclerosis, and generates new hypotheses regarding the role of LDL in atherogenesis...
  31. pmc Sample complexity reduction for two-dimensional electrophoresis using solution isoelectric focusing prefractionation
    Matthew R Richardson
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN, USA
    Electrophoresis 29:2637-44. 2008
    ..5-fold increase over the 1577 spots detected in an unfractionated gel. We concluded that sIEF is an effective means of prefractionation to increase depth of field and improve the analysis of low-abundance proteins...
  32. doi request reprint Protein composition of liver cyst fluid from the BALB/c-cpk/+ mouse model of autosomal recessive polycystic kidney disease
    Xianyin Lai
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Proteomics 9:3775-82. 2009
    ..Protein identifications have been submitted to the PRIDE database (http://www.ebi.ac.uk/pride), accession number 9227...
  33. ncbi request reprint Application of pressure cycling technology to tissue sample preparation for 2-DE
    Heather Ringham
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202 2111, USA
    Electrophoresis 28:1022-4. 2007
    ..In an attempt to improve the yield of detectable proteins during sample preparation, we applied a novel extraction technique called pressure cycling technology...
  34. pmc Variability of protein content in calcium oxalate monohydrate stones
    James C Williams
    Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202 5120, USA
    J Endourol 20:560-4. 2006
    ....
  35. ncbi request reprint Serine/threonine/tyrosine phosphorylation of the LHX3 LIM-homeodomain transcription factor
    Gretchen E Parker
    Department of Biology, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202 5132, USA
    J Cell Biochem 94:67-80. 2005
    ..In conclusion, we have identified unique amino acids within LHX3 that are important for its transcriptional activity and are phosphorylated...
  36. ncbi request reprint The impact of blood contamination on the proteome of cerebrospinal fluid
    Jin Sam You
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, USA
    Proteomics 5:290-6. 2005
    ..This analysis was aimed at identification of proteins found primarily in CSF, those found primarily in blood and assessment of the impact of blood contamination on those proteins found in both fluids...
  37. ncbi request reprint JP-8 jet fuel exposure alters protein expression in the lung
    Matthew G Drake
    Joan B and Donald R Diamond Lung Injury Laboratory, Department of Pediatrics, Arizona Health Sciences Center, 1501 N Campbell Avenue, Room 3354, Tucson, AZ 85724, USA
    Toxicology 191:199-210. 2003
    ..Furthermore, there was a decrease in alpha1-anti-trypsin expression suggesting that JP-8 jet fuel exposure may have implications for the development of pulmonary disorders...
  38. pmc Synaptosome proteomics
    Fengju Bai
    Safety Sciences, Charles River Laboratories Preclinical Services, Worcester, Massachusetts, USA
    Subcell Biochem 43:77-98. 2007
    ....
  39. ncbi request reprint Proteomic and inducible transgenic approaches to study disease processes
    Prabir Ray
    Dorothy P and Richard P Simmons Center for Research and Education in Interstitial Lung Disease, Division of Pulmonary, Allergy, and Critical Care Medicine and Department of Immunology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    Am J Respir Cell Mol Biol 29:S42-6. 2003
  40. ncbi request reprint On the dependence of a characterization of proteomics maps on the number of protein spots considered
    Milan Randić
    National Institute of Chemistry, Ljubljana, Slovenia
    J Chem Inf Model 46:116-22. 2006
    ..We found that proteins maps based on a set of about the 250 most abundant proteins spots suffice for a satisfactory numerical characterization of such maps...
  41. pmc Mitochondrial matrix phosphoproteome: effect of extra mitochondrial calcium
    Rachel K Hopper
    Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892 1061, USA
    Biochemistry 45:2524-36. 2006
    ..These data demonstrate the use of a phosphoproteome screen in determining mitochondrial signaling pathways and reveal new pathways for Ca(2+) modification of mitochondrial function at the level of MnSOD...
  42. ncbi request reprint Proteomics of S-(1, 2-dichlorovinyl)-L-cysteine-induced acute renal failure and autoprotection in mice
    Midhun C Korrapati
    Department of Toxicology, College of Pharmacy, University of Louisiana Monroe, Monroe, Louisiana 71209 0470, USA
    Am J Physiol Renal Physiol 293:F994-F1006. 2007
    ..This study provides the first report on proteomics of DCVC-induced ARF and autoprotection in mice and reflects the application of proteomics in mechanistic studies as well as biomarker development in a variety of toxicological paradigms...
  43. pmc Sample preparation for two-dimensional gel electrophoresis using pressure cycling technology
    Gary B Smejkal
    Proteomics and Small Molecules Applications Laboratory, Pressure BioSciences, Woburn, MA 01801, USA
    Anal Biochem 363:309-11. 2007
  44. ncbi request reprint Proteomics: technologies and applications
    Mark P Molloy
    Pfizer Global Research and Development, Ann Arbor Laboratories, USA
    Brief Funct Genomic Proteomic 1:23-39. 2002
    ..Some areas where proteomics has delivered valuable biological insight will also be considered, and there will be a look towards future areas of development...

Research Grants6

  1. Synaptosomal Protein Changes After EtOH Self-Administration
    Frank Witzmann; Fiscal Year: 2006
    ....
  2. Protein Biomarker Profiles of Abusive Alcohol Consumption
    Frank Witzmann; Fiscal Year: 2007
    ....
  3. Nanoparticle Effects on Epithelial Cell Protein Expression and Function
    Frank A Witzmann; Fiscal Year: 2010
    ..In addition, the studies will correlate the changes in protein expression with changes in cellular function at both toxic and subtoxic levels. ..
  4. Nanoparticle Effects on Epithelial Cell Protein Expression and Function
    Frank Witzmann; Fiscal Year: 2009
    ..In addition, the studies will correlate the changes in protein expression with changes in cellular function at both toxic and subtoxic levels. ..