Stephen R Wassall

Summary

Affiliation: Indiana University
Country: USA

Publications

  1. ncbi request reprint Monounsaturated PE does not phase-separate from the lipid raft molecules sphingomyelin and cholesterol: role for polyunsaturation?
    Saame Raza Shaikh
    Department of Biology, Indiana University Purdue University Indianapolis, 723 West Michigan Street, Indianapolis, IN 46202 5132, USA
    Biochemistry 41:10593-602. 2002
  2. ncbi request reprint Cholesterol hydroxyl group is found to reside in the center of a polyunsaturated lipid membrane
    Thad A Harroun
    National Research Council, Canadian Neutron Beam Centre, Chalk River, Ontario K0J 1J0, Canada
    Biochemistry 45:1227-33. 2006
  3. doi request reprint Docosahexaenoic acid domains: the ultimate non-raft membrane domain
    Stephen R Wassall
    Department of Physics, Indiana University Purdue University Indianapolis, 402 North Blackford Street, Indianapolis, IN 46202 3273, United States
    Chem Phys Lipids 153:57-63. 2008
  4. doi request reprint Solid-state (2)H NMR and MD simulations of positional isomers of a monounsaturated phospholipid membrane: structural implications of double bond location
    Stephen R Wassall
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202 3273, USA
    J Phys Chem B 114:11474-83. 2010
  5. doi request reprint Polyunsaturated fatty acid-cholesterol interactions: domain formation in membranes
    Stephen R Wassall
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202 3273, USA
    Biochim Biophys Acta 1788:24-32. 2009
  6. ncbi request reprint Order from disorder, corralling cholesterol with chaotic lipids. The role of polyunsaturated lipids in membrane raft formation
    Stephen R Wassall
    Department of Physics, Indiana University Purdue University Indianapolis, 402 N Blackford Street, Indianapolis, IN 46202 3273, USA
    Chem Phys Lipids 132:79-88. 2004
  7. ncbi request reprint Controlling membrane cholesterol content. A role for polyunsaturated (docosahexaenoate) phospholipids
    Michael R Brzustowicz
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202 3273, USA
    Biochemistry 41:12509-19. 2002
  8. pmc Docosahexaenoic and eicosapentaenoic acids segregate differently between raft and nonraft domains
    Justin A Williams
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana, USA
    Biophys J 103:228-37. 2012
  9. ncbi request reprint Molecular organization of cholesterol in unsaturated phosphatidylethanolamines: X-ray diffraction and solid state 2H NMR reveal differences with phosphatidylcholines
    Saame Raza Shaikh
    Department of Biology, Indiana University Purdue University Indianapolis, 723 West Michigan Street, Indianapolis, Indiana 46202 5132, USA
    J Am Chem Soc 128:5375-83. 2006
  10. doi request reprint Effect of trans unsaturation on molecular organization in a phospholipid membrane
    Smita P Soni
    Department of Physics, Indiana University Purdue University Indianapolis, 402 North Blackford Street, Indianapolis, Indiana 46202 3273, USA
    Biochemistry 48:11097-107. 2009

Collaborators

Detail Information

Publications21

  1. ncbi request reprint Monounsaturated PE does not phase-separate from the lipid raft molecules sphingomyelin and cholesterol: role for polyunsaturation?
    Saame Raza Shaikh
    Department of Biology, Indiana University Purdue University Indianapolis, 723 West Michigan Street, Indianapolis, IN 46202 5132, USA
    Biochemistry 41:10593-602. 2002
    ..We propose that monounsaturated lipids do not promote formation of stable lipid rafts and that polyunsaturation may be important for raft stability...
  2. ncbi request reprint Cholesterol hydroxyl group is found to reside in the center of a polyunsaturated lipid membrane
    Thad A Harroun
    National Research Council, Canadian Neutron Beam Centre, Chalk River, Ontario K0J 1J0, Canada
    Biochemistry 45:1227-33. 2006
    ..We attribute the change in location to the high disorder of polyunsaturated fatty acids (PUFA) that is incompatible with close proximity to the steroid moiety in its usual "upright" orientation...
  3. doi request reprint Docosahexaenoic acid domains: the ultimate non-raft membrane domain
    Stephen R Wassall
    Department of Physics, Indiana University Purdue University Indianapolis, 402 North Blackford Street, Indianapolis, IN 46202 3273, United States
    Chem Phys Lipids 153:57-63. 2008
    ..According to our model, changes in the conformation of signaling proteins when they move between these disparate domains have the potential to modulate cell function...
  4. doi request reprint Solid-state (2)H NMR and MD simulations of positional isomers of a monounsaturated phospholipid membrane: structural implications of double bond location
    Stephen R Wassall
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202 3273, USA
    J Phys Chem B 114:11474-83. 2010
    ..The simulations support the idea that the order parameter changes reflect differences in molecular surface areas, and furthermore that the molecular areas are a function of the strength of the acyl chain attractions...
  5. doi request reprint Polyunsaturated fatty acid-cholesterol interactions: domain formation in membranes
    Stephen R Wassall
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202 3273, USA
    Biochim Biophys Acta 1788:24-32. 2009
    ..We hypothesize that DHA-rich domains also form in the plasma membrane and are responsible, in part, for the diverse range of health benefits associated with DHA...
  6. ncbi request reprint Order from disorder, corralling cholesterol with chaotic lipids. The role of polyunsaturated lipids in membrane raft formation
    Stephen R Wassall
    Department of Physics, Indiana University Purdue University Indianapolis, 402 N Blackford Street, Indianapolis, IN 46202 3273, USA
    Chem Phys Lipids 132:79-88. 2004
    ..We hypothesize that the enhanced propensity to form SM-/cholesterol-rich rafts as well as PUFA-rich/cholesterol-poor microdomains would modify the function of proteins for which these respective regions provide a platform...
  7. ncbi request reprint Controlling membrane cholesterol content. A role for polyunsaturated (docosahexaenoate) phospholipids
    Michael R Brzustowicz
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202 3273, USA
    Biochemistry 41:12509-19. 2002
    ..Our results form the basis for a model that is consistent with cholesterol's ability to modulate the activity of certain neural transmembrane proteins...
  8. pmc Docosahexaenoic and eicosapentaenoic acids segregate differently between raft and nonraft domains
    Justin A Williams
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana, USA
    Biophys J 103:228-37. 2012
    ..We propose that DHA may be the more bioactive component of fish oil that serves to disrupt lipid raft domain organization. This mechanism represents an evolution in the view of how PUFA remodel membrane architecture...
  9. ncbi request reprint Molecular organization of cholesterol in unsaturated phosphatidylethanolamines: X-ray diffraction and solid state 2H NMR reveal differences with phosphatidylcholines
    Saame Raza Shaikh
    Department of Biology, Indiana University Purdue University Indianapolis, 723 West Michigan Street, Indianapolis, Indiana 46202 5132, USA
    J Am Chem Soc 128:5375-83. 2006
    ..This distinction may have biological implications reflected by the health benefits of dietary polyunsaturated fatty acids that are often taken up into PE > PC...
  10. doi request reprint Effect of trans unsaturation on molecular organization in a phospholipid membrane
    Smita P Soni
    Department of Physics, Indiana University Purdue University Indianapolis, 402 North Blackford Street, Indianapolis, Indiana 46202 3273, USA
    Biochemistry 48:11097-107. 2009
    ....
  11. pmc Molecular organization of cholesterol in polyunsaturated membranes: microdomain formation
    Michael R Brzustowicz
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202 3273, USA
    Biophys J 82:285-98. 2002
    ....
  12. pmc Oleic and docosahexaenoic acid differentially phase separate from lipid raft molecules: a comparative NMR, DSC, AFM, and detergent extraction study
    Saame Raza Shaikh
    Department of Biology, Indiana University Purdue University, Indianapolis, Indiana 46202 5132, USA
    Biophys J 87:1752-66. 2004
    ..We conclude that the OA-PE phase separates from rafts far less than DHA-PE, which may have implications for cellular signaling...
  13. ncbi request reprint Rapid flip-flop in polyunsaturated (docosahexaenoate) phospholipid membranes
    Victoria T Armstrong
    Department of Biology, Indiana University Purdue University Indianapolis, 46202, USA
    Arch Biochem Biophys 414:74-82. 2003
    ..We conclude that PC membranes containing DHA support faster flip-flop and permeability rates than those measured for other less-unsaturated PCs...
  14. pmc Docosahexaenoic acid enhances segregation of lipids between : 2H-NMR study
    Smita P Soni
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202 3273, USA
    Biophys J 95:203-14. 2008
    ..We attribute, in part, the diverse health benefits associated with dietary consumption of DHA to an alteration in membrane domains...
  15. ncbi request reprint Docosahexaenoic acid affects cell signaling by altering lipid rafts
    William Stillwell
    Department of Biology, Indiana University, Purdue University Indianapolis, 723 West Michigan, Indianapolis, IN 46202, USA
    Reprod Nutr Dev 45:559-79. 2005
    ..We conclude with a proposal of how DHA incorporation into membranes may control cell biochemistry and physiology...
  16. ncbi request reprint Docosahexaenoic acid: membrane properties of a unique fatty acid
    William Stillwell
    Department of Biology, Indiana University Purdue University Indianapolis, 723 W Michigan Street, Indianapolis, IN 46202 5132, USA
    Chem Phys Lipids 126:1-27. 2003
    ..It is concluded that DHA's interaction with other membrane lipids, particularly cholesterol, may play a prominent role in modulating the local structure and function of cell membranes...
  17. doi request reprint An electron paramagnetic resonance method for measuring the affinity of a spin-labeled analog of cholesterol for phospholipids
    Justin A Williams
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, IN, 46202 3273, USA
    J Membr Biol 246:689-96. 2013
    ..The virtue of this EPR method is that it provides a measure of chol binding that is quick, employs a commercially available probe and avoids the necessity for physical separation of LUVs and mβCD...
  18. pmc Effects of lipid interactions on model vesicle engulfment by alveolar macrophages
    Matthew J Justice
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana
    Biophys J 106:598-609. 2014
    ....
  19. ncbi request reprint Interaction of cholesterol with a docosahexaenoic acid-containing phosphatidylethanolamine: trigger for microdomain/raft formation?
    Saame Raza Shaikh
    Department of Biology, Indiana University, Indianapolis 46202 5132, USA
    Biochemistry 42:12028-37. 2003
    ..Our findings provide further evidence that cholesterol has a low affinity for DHA-containing phospholipids and that this reduced affinity may serve as a mechanism for triggering the formation of lipid microdomains such as rafts...
  20. doi request reprint Cholesterol is found to reside in the center of a polyunsaturated lipid membrane
    Thad A Harroun
    Department of Physics, Brock University, St Catharines, Ontario L2S 3A1, Canada
    Biochemistry 47:7090-6. 2008
    ..These diffraction measurements unequivocally show cholesterol lies flat in the middle of 20:4-20:4PC bilayers...
  21. ncbi request reprint Cholesterol shows preference for the interior of polyunsaturated lipid membranes
    Siewert J Marrink
    Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Am Chem Soc 130:10-1. 2008
    ....