Research Topics
| WILLIAM H STILLWELLSummaryAffiliation: Indiana University Country: USA Publications
Research Grants
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Detail Information
Publications
Anticancer properties of propofol-docosahexaenoate and propofol-eicosapentaenoate on breast cancer cellsRafat A Siddiqui
Methodist Research Institute, Clarian Health Partners, Indianapolis, IN, USA
Breast Cancer Res 7:R645-54. 2005..The present study describes the synthesis, purification, characterization and evaluation of two novel anticancer conjugates, propofol-docosahexaenoate (propofol-DHA) and propofol-eicosapentaenoate (propofol-EPA)...
Detection of lipid domains in docasahexaenoic acid-rich bilayers by acyl chain-specific FRET probesW Stillwell
Department of Biology, Indiana University Purdue University at Indianapolis, 46202 5132, USA
Chem Phys Lipids 104:113-32. 2000..These experiments indicate that acyl chain affinities may not be sufficient to employ acyl chain-specific N-NBD-PE/N-Rh-PE FRET probes to investigate phase separations into biologically relevant fluid/fluid lipid microdomains...- Docosahexaenoic acid affects cell signaling by altering lipid raftsWilliam Stillwell
Department of Biology, Indiana University, Purdue University Indianapolis, 723 West Michigan, Indianapolis, IN 46202, USA
Reprod Nutr Dev 45:559-79. 2005..We conclude with a proposal of how DHA incorporation into membranes may control cell biochemistry and physiology... - Docosahexaenoic acid: membrane properties of a unique fatty acidWilliam Stillwell
Department of Biology, Indiana University Purdue University Indianapolis, 723 W Michigan Street, Indianapolis, IN 46202 5132, USA
Chem Phys Lipids 126:1-27. 2003..It is concluded that DHA's interaction with other membrane lipids, particularly cholesterol, may play a prominent role in modulating the local structure and function of cell membranes... - Controlling membrane cholesterol content. A role for polyunsaturated (docosahexaenoate) phospholipidsMichael R Brzustowicz
Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202-3273, USA
Biochemistry 41:12509-19. 2002..Our results form the basis for a model that is consistent with cholesterol's ability to modulate the activity of certain neural transmembrane proteins... - Molecular organization of cholesterol in polyunsaturated membranes: microdomain formationMichael R Brzustowicz
Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202-3273, USA
Biophys J 82:285-98. 2002.... 
Docosahexaenoic acid domains: the ultimate non-raft membrane domainStephen R Wassall
Department of Physics, Indiana University Purdue University Indianapolis, 402 North Blackford Street, Indianapolis, IN 46202 3273, United States
Chem Phys Lipids 153:57-63. 2008..According to our model, changes in the conformation of signaling proteins when they move between these disparate domains have the potential to modulate cell function...- Order from disorder, corralling cholesterol with chaotic lipids. The role of polyunsaturated lipids in membrane raft formationStephen R Wassall
Department of Physics, Indiana University Purdue University Indianapolis, 402 N Blackford Street, Indianapolis, IN 46202 3273, USA
Chem Phys Lipids 132:79-88. 2004..We hypothesize that the enhanced propensity to form SM-/cholesterol-rich rafts as well as PUFA-rich/cholesterol-poor microdomains would modify the function of proteins for which these respective regions provide a platform... - Polyunsaturated fatty acid-cholesterol interactions: domain formation in membranesStephen R Wassall
Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202 3273, USA
Biochim Biophys Acta 1788:24-32. 2009..We hypothesize that DHA-rich domains also form in the plasma membrane and are responsible, in part, for the diverse range of health benefits associated with DHA... - Interaction of cholesterol with a docosahexaenoic acid-containing phosphatidylethanolamine: trigger for microdomain/raft formation?Saame Raza Shaikh
Department of Biology, Indiana University, Indianapolis 46202 5132, USA
Biochemistry 42:12028-37. 2003..Our findings provide further evidence that cholesterol has a low affinity for DHA-containing phospholipids and that this reduced affinity may serve as a mechanism for triggering the formation of lipid microdomains such as rafts... - Rapid flip-flop in polyunsaturated (docosahexaenoate) phospholipid membranesVictoria T Armstrong
Department of Biology, Indiana University Purdue University Indianapolis, 46202, USA
Arch Biochem Biophys 414:74-82. 2003..We conclude that PC membranes containing DHA support faster flip-flop and permeability rates than those measured for other less-unsaturated PCs... - Oleic and docosahexaenoic acid differentially phase separate from lipid raft molecules: a comparative NMR, DSC, AFM, and detergent extraction studySaame Raza Shaikh
Department of Biology, Indiana University-Purdue University, Indianapolis, Indiana 46202-5132, USA
Biophys J 87:1752-66. 2004..We conclude that the OA-PE phase separates from rafts far less than DHA-PE, which may have implications for cellular signaling... - Monounsaturated PE does not phase-separate from the lipid raft molecules sphingomyelin and cholesterol: role for polyunsaturation?Saame Raza Shaikh
Department of Biology, Indiana University Purdue University Indianapolis, 723 West Michigan Street, Indianapolis, IN 46202 5132, USA
Biochemistry 41:10593-602. 2002..We propose that monounsaturated lipids do not promote formation of stable lipid rafts and that polyunsaturation may be important for raft stability... - Comparison of cis and trans fatty acid containing phosphatidylcholines on membrane propertiesCharles Roach
Department of Biology, Indiana University Purdue University Indianapolis, 723 West Michigan Street, Indianapolis, Indiana 46202-5132, USA
Biochemistry 43:6344-51. 2004..e., cis double bonds induce much larger membrane perturbations than trans double bonds... - Partitioning of ABA into bilayers of Di-saturated phosphatidylcholines as measured by DSCMichael Katzer
Department of Biology, Indiana University-Purdue University at Indianapolis, Indianapolis 46202, USA
Biophys J 84:314-25. 2003..Differences in partitioning and surface pKa between the biologically active ct-ABA and the inactive tt-isomer are discussed for biological relevance... - Acyl chain unsaturation in PEs modulates phase separation from lipid raft moleculesSaame Raza Shaikh
Department of Biology, Indiana University Purdue University Indianapolis, 723 W Michigan Street, Indianapolis, IN 46202-5132, USA
Biochem Biophys Res Commun 311:793-6. 2003..Our findings demonstrate that increasing unsaturation in PEs results in increased phase separation from SM/CHOL membranes, which may have implications for cellular signaling... - Docosahexaenoic acid enhances segregation of lipids between : 2H-NMR studySmita P Soni
Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202 3273, USA
Biophys J 95:203-14. 2008..We attribute, in part, the diverse health benefits associated with dietary consumption of DHA to an alteration in membrane domains... - Modulation of lipid rafts by Omega-3 fatty acids in inflammation and cancer: implications for use of lipids during nutrition supportRafat A Siddiqui
Methodist Research Institute, Cellular Biochemistry, Indianapolis, IN 46202, USA
Nutr Clin Pract 22:74-88. 2007..We review how alteration of raft lipids affects the raft/nonraft localization and hence the function of several proteins involved in cell signaling. We focus our discussion of raft-signaling proteins on inflammation and cancer... - Inhibition of phenylephrine-induced cardiac hypertrophy by docosahexaenoic acidRafat A Siddiqui
Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, 1701 N Senate Blvd, Indianapolis, Indiana 46202, USA
J Cell Biochem 92:1141-59. 2004..Our results suggest that inhibition of Ras --> Raf-1 --> Erk1/2 --> p90(rsk) --> hypertrophy is one possible pathway by which DHA can inhibit cardiac hypertrophy. In vivo studies are needed to confirm these in vitro effects of DHA... - Synthesis of a novel phosphatidylcholine conjugated to docosahexaenoic acid and methotrexate that inhibits cell proliferationMustapha Zerouga
Department of Biology, Indiana University-Purdue University, Indianapolis, IN 46202-5143, USA
Anticancer Drugs 13:301-11. 2002..These data show the feasibility of synthesizing, and the inhibitory activity of phosphatidylcholine with DHA in the sn-1 position and MTX in the sn-2 position, and suggest the compound's potential use in cancer chemotherapy... - Anticancer properties of oxidation products of docosahexaenoic acidRafat A Siddiqui
Methodist Research Institute, Clarian Health Partners, Indianapolis, IN 46202, United States
Chem Phys Lipids 153:47-56. 2008..We conclude that the study of the effects of lipid peroxidation products on biochemical function will be a difficult but highly rewarding area for future studies... - Molecular organization of cholesterol in unsaturated phosphatidylethanolamines: X-ray diffraction and solid state 2H NMR reveal differences with phosphatidylcholinesSaame Raza Shaikh
Department of Biology, Indiana University Purdue University Indianapolis, 723 West Michigan Street, Indianapolis, Indiana 46202-5132, USA
J Am Chem Soc 128:5375-83. 2006..This distinction may have biological implications reflected by the health benefits of dietary polyunsaturated fatty acids that are often taken up into PE > PC... - Omega 3-fatty acids: health benefits and cellular mechanisms of actionRafat A Siddiqui
Methodist Research Institute, Indianapolis, Indiana 46202, USA
Mini Rev Med Chem 4:859-71. 2004..Protein phosphatases are also involved with the protein Bcl2, which regulates the release of cytochrome c from mitochondria, and eventually, activation of the apoptotic enzyme caspase 3... - Cell-cycle arrest in Jurkat leukaemic cells: a possible role for docosahexaenoic acidRafat A Siddiqui
Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, 1701 N Senate Boulevard, Indianapolis, IN 46202, USA
Biochem J 371:621-9. 2003..The net effect of cdk2 inhibition and protein phosphatase activation is an inhibition of pRb phosphorylation, consequently arresting Jurkat cell growth... - International workshop on cellular and molecular aspects of omega-3 fatty acids and cancerWilliam Stillwell
Department of Biology, Indiana University-Purdue University, 723 W. Michigan Street, Indianapolis, IN 46202-5132, USA
J Lipid Res 43:1579-80. 2002
Research Grants
- OMEGA-3 FATTY ACIDS & TUMOR MEMBRANE STRUCTURE/FUNCTIONWilliam Stillwell; Fiscal Year: 1993..From these experiments we anticipate being able to describe for the first time one underlying fundamental mechanism of action of omega-3 fatty acids as anti-cancer agents...
- Omega 3 Fatty Acids & Tumor Membrane Structure/ FunctionWilliam Stillwell; Fiscal Year: 2004..These results will set the stage for creating novel methods of activating tumor-reactive CTL in vitro for the purpose of generating memory CTL to patrol for cancer cells in vivo. ..
- OMEGA 3 FATTY ACIDS & TUMOR MEMBRANE STRUCTURE/FUNCTIONWilliam Stillwell; Fiscal Year: 1999..Successfully unraveling DHA's mode of action should suggest practical clinical, dietary or therapeutic applications for this potentially exciting, useful natural substance. ..