WILLIAM H STILLWELL

Summary

Affiliation: Indiana University
Country: USA

Publications

  1. pmc Anticancer properties of propofol-docosahexaenoate and propofol-eicosapentaenoate on breast cancer cells
    Rafat A Siddiqui
    Methodist Research Institute, Clarian Health Partners, Indianapolis, IN, USA
    Breast Cancer Res 7:R645-54. 2005
  2. ncbi request reprint Detection of lipid domains in docasahexaenoic acid-rich bilayers by acyl chain-specific FRET probes
    W Stillwell
    Department of Biology, Indiana University Purdue University at Indianapolis, 46202 5132, USA
    Chem Phys Lipids 104:113-32. 2000
  3. ncbi request reprint Docosahexaenoic acid affects cell signaling by altering lipid rafts
    William Stillwell
    Department of Biology, Indiana University, Purdue University Indianapolis, 723 West Michigan, Indianapolis, IN 46202, USA
    Reprod Nutr Dev 45:559-79. 2005
  4. ncbi request reprint Docosahexaenoic acid: membrane properties of a unique fatty acid
    William Stillwell
    Department of Biology, Indiana University Purdue University Indianapolis, 723 W Michigan Street, Indianapolis, IN 46202 5132, USA
    Chem Phys Lipids 126:1-27. 2003
  5. ncbi request reprint Controlling membrane cholesterol content. A role for polyunsaturated (docosahexaenoate) phospholipids
    Michael R Brzustowicz
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202 3273, USA
    Biochemistry 41:12509-19. 2002
  6. pmc Molecular organization of cholesterol in polyunsaturated membranes: microdomain formation
    Michael R Brzustowicz
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202 3273, USA
    Biophys J 82:285-98. 2002
  7. doi request reprint Docosahexaenoic acid domains: the ultimate non-raft membrane domain
    Stephen R Wassall
    Department of Physics, Indiana University Purdue University Indianapolis, 402 North Blackford Street, Indianapolis, IN 46202 3273, United States
    Chem Phys Lipids 153:57-63. 2008
  8. ncbi request reprint Order from disorder, corralling cholesterol with chaotic lipids. The role of polyunsaturated lipids in membrane raft formation
    Stephen R Wassall
    Department of Physics, Indiana University Purdue University Indianapolis, 402 N Blackford Street, Indianapolis, IN 46202 3273, USA
    Chem Phys Lipids 132:79-88. 2004
  9. doi request reprint Polyunsaturated fatty acid-cholesterol interactions: domain formation in membranes
    Stephen R Wassall
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202 3273, USA
    Biochim Biophys Acta 1788:24-32. 2009
  10. ncbi request reprint Interaction of cholesterol with a docosahexaenoic acid-containing phosphatidylethanolamine: trigger for microdomain/raft formation?
    Saame Raza Shaikh
    Department of Biology, Indiana University, Indianapolis 46202 5132, USA
    Biochemistry 42:12028-37. 2003

Research Grants

Collaborators

Detail Information

Publications25

  1. pmc Anticancer properties of propofol-docosahexaenoate and propofol-eicosapentaenoate on breast cancer cells
    Rafat A Siddiqui
    Methodist Research Institute, Clarian Health Partners, Indianapolis, IN, USA
    Breast Cancer Res 7:R645-54. 2005
    ..The present study describes the synthesis, purification, characterization and evaluation of two novel anticancer conjugates, propofol-docosahexaenoate (propofol-DHA) and propofol-eicosapentaenoate (propofol-EPA)...
  2. ncbi request reprint Detection of lipid domains in docasahexaenoic acid-rich bilayers by acyl chain-specific FRET probes
    W Stillwell
    Department of Biology, Indiana University Purdue University at Indianapolis, 46202 5132, USA
    Chem Phys Lipids 104:113-32. 2000
    ..These experiments indicate that acyl chain affinities may not be sufficient to employ acyl chain-specific N-NBD-PE/N-Rh-PE FRET probes to investigate phase separations into biologically relevant fluid/fluid lipid microdomains...
  3. ncbi request reprint Docosahexaenoic acid affects cell signaling by altering lipid rafts
    William Stillwell
    Department of Biology, Indiana University, Purdue University Indianapolis, 723 West Michigan, Indianapolis, IN 46202, USA
    Reprod Nutr Dev 45:559-79. 2005
    ..We conclude with a proposal of how DHA incorporation into membranes may control cell biochemistry and physiology...
  4. ncbi request reprint Docosahexaenoic acid: membrane properties of a unique fatty acid
    William Stillwell
    Department of Biology, Indiana University Purdue University Indianapolis, 723 W Michigan Street, Indianapolis, IN 46202 5132, USA
    Chem Phys Lipids 126:1-27. 2003
    ..It is concluded that DHA's interaction with other membrane lipids, particularly cholesterol, may play a prominent role in modulating the local structure and function of cell membranes...
  5. ncbi request reprint Controlling membrane cholesterol content. A role for polyunsaturated (docosahexaenoate) phospholipids
    Michael R Brzustowicz
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202 3273, USA
    Biochemistry 41:12509-19. 2002
    ..Our results form the basis for a model that is consistent with cholesterol's ability to modulate the activity of certain neural transmembrane proteins...
  6. pmc Molecular organization of cholesterol in polyunsaturated membranes: microdomain formation
    Michael R Brzustowicz
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202 3273, USA
    Biophys J 82:285-98. 2002
    ....
  7. doi request reprint Docosahexaenoic acid domains: the ultimate non-raft membrane domain
    Stephen R Wassall
    Department of Physics, Indiana University Purdue University Indianapolis, 402 North Blackford Street, Indianapolis, IN 46202 3273, United States
    Chem Phys Lipids 153:57-63. 2008
    ..According to our model, changes in the conformation of signaling proteins when they move between these disparate domains have the potential to modulate cell function...
  8. ncbi request reprint Order from disorder, corralling cholesterol with chaotic lipids. The role of polyunsaturated lipids in membrane raft formation
    Stephen R Wassall
    Department of Physics, Indiana University Purdue University Indianapolis, 402 N Blackford Street, Indianapolis, IN 46202 3273, USA
    Chem Phys Lipids 132:79-88. 2004
    ..We hypothesize that the enhanced propensity to form SM-/cholesterol-rich rafts as well as PUFA-rich/cholesterol-poor microdomains would modify the function of proteins for which these respective regions provide a platform...
  9. doi request reprint Polyunsaturated fatty acid-cholesterol interactions: domain formation in membranes
    Stephen R Wassall
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202 3273, USA
    Biochim Biophys Acta 1788:24-32. 2009
    ..We hypothesize that DHA-rich domains also form in the plasma membrane and are responsible, in part, for the diverse range of health benefits associated with DHA...
  10. ncbi request reprint Interaction of cholesterol with a docosahexaenoic acid-containing phosphatidylethanolamine: trigger for microdomain/raft formation?
    Saame Raza Shaikh
    Department of Biology, Indiana University, Indianapolis 46202 5132, USA
    Biochemistry 42:12028-37. 2003
    ..Our findings provide further evidence that cholesterol has a low affinity for DHA-containing phospholipids and that this reduced affinity may serve as a mechanism for triggering the formation of lipid microdomains such as rafts...
  11. ncbi request reprint Rapid flip-flop in polyunsaturated (docosahexaenoate) phospholipid membranes
    Victoria T Armstrong
    Department of Biology, Indiana University Purdue University Indianapolis, 46202, USA
    Arch Biochem Biophys 414:74-82. 2003
    ..We conclude that PC membranes containing DHA support faster flip-flop and permeability rates than those measured for other less-unsaturated PCs...
  12. pmc Oleic and docosahexaenoic acid differentially phase separate from lipid raft molecules: a comparative NMR, DSC, AFM, and detergent extraction study
    Saame Raza Shaikh
    Department of Biology, Indiana University Purdue University, Indianapolis, Indiana 46202 5132, USA
    Biophys J 87:1752-66. 2004
    ..We conclude that the OA-PE phase separates from rafts far less than DHA-PE, which may have implications for cellular signaling...
  13. ncbi request reprint Monounsaturated PE does not phase-separate from the lipid raft molecules sphingomyelin and cholesterol: role for polyunsaturation?
    Saame Raza Shaikh
    Department of Biology, Indiana University Purdue University Indianapolis, 723 West Michigan Street, Indianapolis, IN 46202 5132, USA
    Biochemistry 41:10593-602. 2002
    ..We propose that monounsaturated lipids do not promote formation of stable lipid rafts and that polyunsaturation may be important for raft stability...
  14. ncbi request reprint Comparison of cis and trans fatty acid containing phosphatidylcholines on membrane properties
    Charles Roach
    Department of Biology, Indiana University Purdue University Indianapolis, 723 West Michigan Street, Indianapolis, Indiana 46202 5132, USA
    Biochemistry 43:6344-51. 2004
    ..e., cis double bonds induce much larger membrane perturbations than trans double bonds...
  15. pmc Partitioning of ABA into bilayers of Di-saturated phosphatidylcholines as measured by DSC
    Michael Katzer
    Department of Biology, Indiana University Purdue University at Indianapolis, Indianapolis 46202, USA
    Biophys J 84:314-25. 2003
    ..Differences in partitioning and surface pKa between the biologically active ct-ABA and the inactive tt-isomer are discussed for biological relevance...
  16. ncbi request reprint Acyl chain unsaturation in PEs modulates phase separation from lipid raft molecules
    Saame Raza Shaikh
    Department of Biology, Indiana University Purdue University Indianapolis, 723 W Michigan Street, Indianapolis, IN 46202 5132, USA
    Biochem Biophys Res Commun 311:793-6. 2003
    ..Our findings demonstrate that increasing unsaturation in PEs results in increased phase separation from SM/CHOL membranes, which may have implications for cellular signaling...
  17. pmc Docosahexaenoic acid enhances segregation of lipids between : 2H-NMR study
    Smita P Soni
    Department of Physics, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 46202 3273, USA
    Biophys J 95:203-14. 2008
    ..We attribute, in part, the diverse health benefits associated with dietary consumption of DHA to an alteration in membrane domains...
  18. ncbi request reprint Modulation of lipid rafts by Omega-3 fatty acids in inflammation and cancer: implications for use of lipids during nutrition support
    Rafat A Siddiqui
    Methodist Research Institute, Cellular Biochemistry, Indianapolis, IN 46202, USA
    Nutr Clin Pract 22:74-88. 2007
    ..We review how alteration of raft lipids affects the raft/nonraft localization and hence the function of several proteins involved in cell signaling. We focus our discussion of raft-signaling proteins on inflammation and cancer...
  19. ncbi request reprint Inhibition of phenylephrine-induced cardiac hypertrophy by docosahexaenoic acid
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, 1701 N Senate Blvd, Indianapolis, Indiana 46202, USA
    J Cell Biochem 92:1141-59. 2004
    ..Our results suggest that inhibition of Ras --> Raf-1 --> Erk1/2 --> p90(rsk) --> hypertrophy is one possible pathway by which DHA can inhibit cardiac hypertrophy. In vivo studies are needed to confirm these in vitro effects of DHA...
  20. ncbi request reprint Synthesis of a novel phosphatidylcholine conjugated to docosahexaenoic acid and methotrexate that inhibits cell proliferation
    Mustapha Zerouga
    Department of Biology, Indiana University Purdue University, Indianapolis, IN 46202 5143, USA
    Anticancer Drugs 13:301-11. 2002
    ..These data show the feasibility of synthesizing, and the inhibitory activity of phosphatidylcholine with DHA in the sn-1 position and MTX in the sn-2 position, and suggest the compound's potential use in cancer chemotherapy...
  21. doi request reprint Anticancer properties of oxidation products of docosahexaenoic acid
    Rafat A Siddiqui
    Methodist Research Institute, Clarian Health Partners, Indianapolis, IN 46202, United States
    Chem Phys Lipids 153:47-56. 2008
    ..We conclude that the study of the effects of lipid peroxidation products on biochemical function will be a difficult but highly rewarding area for future studies...
  22. ncbi request reprint Molecular organization of cholesterol in unsaturated phosphatidylethanolamines: X-ray diffraction and solid state 2H NMR reveal differences with phosphatidylcholines
    Saame Raza Shaikh
    Department of Biology, Indiana University Purdue University Indianapolis, 723 West Michigan Street, Indianapolis, Indiana 46202 5132, USA
    J Am Chem Soc 128:5375-83. 2006
    ..This distinction may have biological implications reflected by the health benefits of dietary polyunsaturated fatty acids that are often taken up into PE > PC...
  23. ncbi request reprint Omega 3-fatty acids: health benefits and cellular mechanisms of action
    Rafat A Siddiqui
    Methodist Research Institute, Indianapolis, Indiana 46202, USA
    Mini Rev Med Chem 4:859-71. 2004
    ..Protein phosphatases are also involved with the protein Bcl2, which regulates the release of cytochrome c from mitochondria, and eventually, activation of the apoptotic enzyme caspase 3...
  24. pmc Cell-cycle arrest in Jurkat leukaemic cells: a possible role for docosahexaenoic acid
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, 1701 N Senate Boulevard, Indianapolis, IN 46202, USA
    Biochem J 371:621-9. 2003
    ..The net effect of cdk2 inhibition and protein phosphatase activation is an inhibition of pRb phosphorylation, consequently arresting Jurkat cell growth...
  25. ncbi request reprint International workshop on cellular and molecular aspects of omega-3 fatty acids and cancer
    William Stillwell
    Department of Biology, Indiana University Purdue University, 723 W Michigan Street, Indianapolis, IN 46202 5132, USA
    J Lipid Res 43:1579-80. 2002

Research Grants13

  1. OMEGA-3 FATTY ACIDS & TUMOR MEMBRANE STRUCTURE/FUNCTION
    William Stillwell; Fiscal Year: 1993
    ..From these experiments we anticipate being able to describe for the first time one underlying fundamental mechanism of action of omega-3 fatty acids as anti-cancer agents...
  2. Omega 3 Fatty Acids & Tumor Membrane Structure/ Function
    William Stillwell; Fiscal Year: 2004
    ..These results will set the stage for creating novel methods of activating tumor-reactive CTL in vitro for the purpose of generating memory CTL to patrol for cancer cells in vivo. ..
  3. OMEGA 3 FATTY ACIDS & TUMOR MEMBRANE STRUCTURE/FUNCTION
    William Stillwell; Fiscal Year: 1999
    ..Successfully unraveling DHA's mode of action should suggest practical clinical, dietary or therapeutic applications for this potentially exciting, useful natural substance. ..