Hal Broxmeyer

Summary

Affiliation: Indiana University
Country: USA

Publications

  1. Broxmeyer H, Franklin D, Cooper S, Hangoc G, Mantel C. Cyclin dependent kinase inhibitors differentially modulate synergistic cytokine responsiveness of hematopoietic progenitor cells. Stem Cells Dev. 2012;21:1597-603 pubmed publisher
    ..These findings underscore the complex interplay of cell cycle regulators in HPC, and demonstrate that loss of one can sometimes be compensated by loss of another CDKI in both, a pro- or anti-proliferative context. ..
  2. Broxmeyer H, Orschell C, Clapp D, Hangoc G, Cooper S, Plett P, et al. Rapid mobilization of murine and human hematopoietic stem and progenitor cells with AMD3100, a CXCR4 antagonist. J Exp Med. 2005;201:1307-18 pubmed
    ..These results support the hypothesis that the CXCL12-CXCR4 axis is involved in marrow retention of HSCs and HPCs, and demonstrate the clinical potential of AMD3100 for HSC mobilization. ..
  3. Capitano M, Zhao L, Cooper S, Thorsheim C, Suzuki A, Huang X, et al. Phosphatidylinositol transfer proteins regulate megakaryocyte TGF-?1 secretion and hematopoiesis in mice. Blood. 2018;: pubmed publisher
    ..Altogether, these data show that ablating Pitps in MKs indirectly dysregulates hematopoiesis in the BM by disrupting ?-granule physiology and secretion of TGF-?1. ..
  4. Huang X, Guo B, Liu S, Wan J, Broxmeyer H. Neutralizing negative epigenetic regulation by HDAC5 enhances human haematopoietic stem cell homing and engraftment. Nat Commun. 2018;9:2741 pubmed publisher
    ..These results demonstrate a previously unknown negative epigenetic regulation of HSC homing and engraftment by HDAC5, and allow for a new and simple translational strategy to enhance HSC transplantation. ..
  5. Broxmeyer H, Mor Vaknin N, Kappes F, Legendre M, Saha A, Ou X, et al. Concise review: role of DEK in stem/progenitor cell biology. Stem Cells. 2013;31:1447-53 pubmed publisher
  6. Broxmeyer H. Erythropoietin: multiple targets, actions, and modifying influences for biological and clinical consideration. J Exp Med. 2013;210:205-8 pubmed publisher
  7. Capitano M, Chitteti B, Cooper S, Srour E, Bartke A, Broxmeyer H. Ames hypopituitary dwarf mice demonstrate imbalanced myelopoiesis between bone marrow and spleen. Blood Cells Mol Dis. 2015;55:15-20 pubmed publisher
    ..The reasons for this unusual shift in spleen to marrow hematopoiesis in Ames dwarf mice are yet to be determined but may relate to the decreased hormone levels in these mice. ..
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    Broxmeyer H, Youn B, Kim C, Hangoc G, Cooper S, Mantel C. Chemokine regulation of hematopoiesis and the involvement of pertussis toxin-sensitive G alpha i proteins. Ann N Y Acad Sci. 2001;938:117-27; discussion 127-8 pubmed
    ..These results suggest that not all chemokine-mediated effects on MPCs are necessarily mediated through pertussis toxin-sensitive G alpha i proteins. ..
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    Broxmeyer H, Kohli L, Kim C, Lee Y, Mantel C, Cooper S, et al. Stromal cell-derived factor-1/CXCL12 directly enhances survival/antiapoptosis of myeloid progenitor cells through CXCR4 and G(alpha)i proteins and enhances engraftment of competitive, repopulating stem cells. J Leukoc Biol. 2003;73:630-8 pubmed
    ..These results extend information on the survival effects mediated through the SDF-1/CXCL12-CXCR4 axis and may be of relevance for ex vivo expansion and gene-transduction procedures. ..

More Information

Publications24

  1. Broxmeyer H. Enhancing the efficacy of engraftment of cord blood for hematopoietic cell transplantation. Transfus Apher Sci. 2016;54:364-72 pubmed publisher
    ..The ultimate goal is to manipulate efficiency and efficacy for safe and economical use of single unit CB HCT. ..
  2. Cai Q, Capitano M, Huang X, Guo B, Cooper S, Broxmeyer H. Combinations of antioxidants and/or of epigenetic enzyme inhibitors allow for enhanced collection of mouse bone marrow hematopoietic stem cells in ambient air. Blood Cells Mol Dis. 2018;71:23-28 pubmed publisher
  3. Messina Graham S, Broxmeyer H. SDF-1/CXCL12 modulates mitochondrial respiration of immature blood cells in a bi-phasic manner. Blood Cells Mol Dis. 2016;58:13-8 pubmed publisher
  4. Mantel C, O Leary H, Chitteti B, Huang X, Cooper S, Hangoc G, et al. Enhancing Hematopoietic Stem Cell Transplantation Efficacy by Mitigating Oxygen Shock. Cell. 2015;161:1553-65 pubmed publisher
    ..Mitigating EPHOSS during cell collection and processing by pharmacological means may be clinically advantageous for transplantation. ..
  5. Broxmeyer H, Li J, Hangoc G, Cooper S, Tao W, Mantel C, et al. Regulation of myeloid progenitor cell proliferation/survival by IL-31 receptor and IL-31. Exp Hematol. 2007;35:78-86 pubmed
    ..Under steady-state conditions, loss of IL-31R on HPC does not appear to influence the activity of competitive repopulating HSC. These results with HPC may be of future utility for manipulation of hematopoiesis in a preclinical setting. ..
  6. Broxmeyer H, Cooper S, Hangoc G, Gao J, Murphy P. Dominant myelopoietic effector functions mediated by chemokine receptor CCR1. J Exp Med. 1999;189:1987-92 pubmed
    ..CCR1 is not a dominant receptor for MIP-1alpha suppression of MPC proliferation, but it does negatively impact G-CSF-induced MPC mobilization. ..
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    Broxmeyer H, Cooper S, Kohli L, Hangoc G, Lee Y, Mantel C, et al. Transgenic expression of stromal cell-derived factor-1/CXC chemokine ligand 12 enhances myeloid progenitor cell survival/antiapoptosis in vitro in response to growth factor withdrawal and enhances myelopoiesis in vivo. J Immunol. 2003;170:421-9 pubmed
    ..These results substantiate survival effects of SDF-1/CXCL12, now extended to progenitors engineered to endogenously produce low levels of this cytokine, and demonstrate activity in vivo for SDF-1/CXCL12 in addition to cell trafficking. ..
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    Broxmeyer H, Cooper S, Hangoc G, Kim C. Stromal cell-derived factor-1/CXCL12 selectively counteracts inhibitory effects of myelosuppressive chemokines on hematopoietic progenitor cell proliferation in vitro. Stem Cells Dev. 2005;14:199-203 pubmed
    ..Thus, SDF-1/CXCL12 differentially and selectively regulates suppression of HPC proliferation by chemokines. These effects may counter myelosuppressive effects of certain chemokines in vivo, where proliferation of HPCs must be sustained. ..
  9. Lee M, Mantel C, Lee S, Moon S, Broxmeyer H. MiR-31/SDHA Axis Regulates Reprogramming Efficiency through Mitochondrial Metabolism. Stem Cell Reports. 2016;7:1-10 pubmed publisher
    ..This is supportive of multi-stage reprogramming whereby metabolic remodeling is fundamental. ..
  10. Broxmeyer H, Hoggatt J, O Leary H, Mantel C, Chitteti B, Cooper S, et al. Dipeptidylpeptidase 4 negatively regulates colony-stimulating factor activity and stress hematopoiesis. Nat Med. 2012;18:1786-96 pubmed publisher
    ..DPP4 inhibition enhanced engraftment in mice without compromising HSC function, suggesting the potential clinical utility of this approach. ..
  11. Broxmeyer H, Christopherson K, Hangoc G, Cooper S, Mantel C, Renukaradhya G, et al. CD1d expression on and regulation of murine hematopoietic stem and progenitor cells. Blood. 2012;119:5731-41 pubmed publisher
    ..Pretreatment of normal BM cells with CD1d Abs greatly enhanced their engraftment of HSCs. The results of the present study implicate CD1d in a previously unrecognized regulatory role of normal and stressed hematopoiesis. ..
  12. Broxmeyer H, Kappes F, Mor Vaknin N, Legendre M, Kinzfogl J, Cooper S, et al. DEK regulates hematopoietic stem engraftment and progenitor cell proliferation. Stem Cells Dev. 2012;21:1449-54 pubmed publisher
    ..This demonstrates that DEK has potent effects on HSCs, HPCs, and hematopoiesis, information of biological and potential clinical interest. ..
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    Broxmeyer H, Mejia J, Hangoc G, Barese C, Dinauer M, Cooper S. SDF-1/CXCL12 enhances in vitro replating capacity of murine and human multipotential and macrophage progenitor cells. Stem Cells Dev. 2007;16:589-96 pubmed
    ..However, the results suggest that SDF-1/CXCL12 enhances in vitro replating/self-renewal of HPCs, which may contribute to myelopoiesis in vivo. This information may be of value to ex vivo expansion of HPCs/HSCs. ..
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    Broxmeyer H, Hangoc G, Cooper S, Campbell T, Ito S, Mantel C. AMD3100 and CD26 modulate mobilization, engraftment, and survival of hematopoietic stem and progenitor cells mediated by the SDF-1/CXCL12-CXCR4 axis. Ann N Y Acad Sci. 2007;1106:1-19 pubmed
    ..These results demonstrate the means to increase the mobilization of HPCs, the engrafting capability of HSCs, and responsiveness of HPCs to the survival-enhancing activity of SDF-1/CXCL12, effects that may be of practical value. ..
  15. Broxmeyer H, Srour E, Cooper S, Wallace C, Hangoc G, Youn B. Angiopoietin-like-2 and -3 act through their coiled-coil domains to enhance survival and replating capacity of human cord blood hematopoietic progenitors. Blood Cells Mol Dis. 2012;48:25-9 pubmed publisher
    ..Thus, among ANGPTL family members, ANGPTL-2 and -3 had enhancing activities on human HPC survival and replating activity, effects requiring the CC domain of the ANGPTL molecules. This information is of relevance to hu HPC regulation. ..