Stephen D Bell
Affiliation: Indiana University
Bell S. Initiating DNA replication: a matter of prime importance. Biochem Soc Trans. 2019;: pubmed publisher
..Significantly, the general conclusions from these studies likely extend to a broad class of extrachromosomal element-associated primases as well as the human primase-related DNA repair enzyme, PrimPol. ..
Yan J, Holzer S, Pellegrini L, Bell S. An archaeal primase functions as a nanoscale caliper to define primer length. Proc Natl Acad Sci U S A. 2018;115:6697-6702 pubmed publisher
..In addition, we determine the key determinants that govern primer length definition by the archaeal primase. Our results indicate a primer measuring system that functions akin to a caliper. ..
Samson R, Abeyrathne P, Bell S. Mechanism of Archaeal MCM Helicase Recruitment to DNA Replication Origins. Mol Cell. 2016;61:287-96 pubmed publisher
..Additionally, we provide evidence that an open-ring form of the archaeal MCM homohexamer is loaded at origins. ..
Samson R, Bell S. Archaeal DNA Replication Origins and Recruitment of the MCM Replicative Helicase. Enzymes. 2016;39:169-90 pubmed publisher
..In this chapter, we will describe recent advances in our understanding of the basis of archaeal origin definition and how the archaeal initiator proteins recruit the replicative helicase to origins. ..
Bell S. Initiation of DNA Replication in the Archaea. Adv Exp Med Biol. 2017;1042:99-115 pubmed publisher
..Genetic, structural, and biochemical studies have revealed the nature of archaeal origin specification. Recent work has begun to shed light on the mechanisms of replication initiation in these organisms. ..
Liew L, Lim Z, Cohen M, Kong Z, Marjavaara L, Chabes A, et al
. Hydroxyurea-Mediated Cytotoxicity Without Inhibition of Ribonucleotide Reductase. Cell Rep. 2016;17:1657-1670 pubmed publisher
..Furthermore, we find evidence for a detoxification response induced by HU treatment. ..
Xu Y, Gristwood T, Hodgson B, Trinidad J, Albers S, Bell S. Archaeal orthologs of Cdc45 and GINS form a stable complex that stimulates the helicase activity of MCM. Proc Natl Acad Sci U S A. 2016;113:13390-13395 pubmed
..Further, the archaeal GINSâ€¢Cdc45 complex contains two copies of Cdc45. Our analyses give insight into the function and evolution of the conserved core of the archaeal/eukaryotic replisome. ..
Samson R, Dobro M, Jensen G, Bell S. The Structure, Function and Roles of the Archaeal ESCRT Apparatus. Subcell Biochem. 2017;84:357-377 pubmed publisher
..Perhaps surprisingly, given their prokaryotic morphology, some archaea also possess a core cell division apparatus that is related to that involved in the final stages of membrane abscission in vertebrate cells, the ESCRT machinery. ..
Holzer S, Yan J, Kilkenny M, Bell S, Pellegrini L. Primer synthesis by a eukaryotic-like archaeal primase is independent of its Fe-S cluster. Nat Commun. 2017;8:1718 pubmed publisher
..Our evidence shows that PriX has replaced PriL as the subunit that endows primase with the unique ability to initiate nucleic acid synthesis. Importantly, our findings reveal that the Fe-S cluster is not required for primer synthesis. ..