Erik C Ralph

Summary

Affiliation: Human BioMolecular Research Institute
Country: USA

Publications

  1. pmc His-tag truncated butyrylcholinesterase as a useful construct for in vitro characterization of wild-type and variant butyrylcholinesterases
    Erik C Ralph
    Human BioMolecular Research Institute, 5310 Eastgate Mall, San Diego, CA 92121, USA
    Protein Expr Purif 80:22-7. 2011
  2. doi Nonquaternary reactivators for organophosphate-inhibited cholinesterases
    Jarosław Kalisiak
    Human BioMolecular Research Institute, 5310 Eastgate Mall, San Diego, California 92121, United States
    J Med Chem 55:465-74. 2012
  3. doi Amidine-oximes: reactivators for organophosphate exposure
    Jarosław Kalisiak
    Human BioMolecular Research Institute, 5310 Eastgate Mall, San Diego, California 92121, United States
    J Med Chem 54:3319-30. 2011
  4. pmc Identification of human butyrylcholinesterase organophosphate-resistant variants through a novel mammalian enzyme functional screen
    Jun Zhang
    Human BioMolecular Research Institute, San Diego, CA 92121, USA
    J Pharmacol Exp Ther 343:673-82. 2012
  5. doi pH dependence on functional activity of human and mouse flavin-containing monooxygenase 5
    Meike S Motika
    Human BioMolecular Research Institute, 5310 Eastgate Mall, San Diego, CA 92121, USA
    Biochem Pharmacol 83:962-8. 2012
  6. pmc Characterization of human flavin-containing monooxygenase (FMO) 3 and FMO5 expressed as maltose-binding protein fusions
    Robert R Reddy
    Human BioMolecular Research Institute, 5310 Eastgate Mall, San Diego, CA 92121, USA
    Drug Metab Dispos 38:2239-45. 2010

Collaborators

  • Jarosław Kalisiak
  • Jun Zhang
  • John R Cashman
  • Meike S Motika
  • Robert R Reddy
  • Mary Dwyer
  • Sigeng Chen
  • Mary A Dwyer

Detail Information

Publications6

  1. pmc His-tag truncated butyrylcholinesterase as a useful construct for in vitro characterization of wild-type and variant butyrylcholinesterases
    Erik C Ralph
    Human BioMolecular Research Institute, 5310 Eastgate Mall, San Diego, CA 92121, USA
    Protein Expr Purif 80:22-7. 2011
    ....
  2. doi Nonquaternary reactivators for organophosphate-inhibited cholinesterases
    Jarosław Kalisiak
    Human BioMolecular Research Institute, 5310 Eastgate Mall, San Diego, California 92121, United States
    J Med Chem 55:465-74. 2012
    ..e., a dose of 36 μmol/kg, i.p.), 7b and 12e protected 100% of the animals challenged with the sarin nerve agent model compound that caused lethality in 6/11 animals without amidine-oxime...
  3. doi Amidine-oximes: reactivators for organophosphate exposure
    Jarosław Kalisiak
    Human BioMolecular Research Institute, 5310 Eastgate Mall, San Diego, California 92121, United States
    J Med Chem 54:3319-30. 2011
    ..In addition, 15d was tested in a post-treatment experiment (145 μmol/kg, ip, administration 5 min after sarin model compound exposure). In both cases, amidine-oximes afforded 100% 24 h survival in an animal model of OP exposure...
  4. pmc Identification of human butyrylcholinesterase organophosphate-resistant variants through a novel mammalian enzyme functional screen
    Jun Zhang
    Human BioMolecular Research Institute, San Diego, CA 92121, USA
    J Pharmacol Exp Ther 343:673-82. 2012
    ..The mammalian functional screening approach can serve as a cornerstone for further optimization and screening for OP-resistant hBChEs for potential therapeutic applications...
  5. doi pH dependence on functional activity of human and mouse flavin-containing monooxygenase 5
    Meike S Motika
    Human BioMolecular Research Institute, 5310 Eastgate Mall, San Diego, CA 92121, USA
    Biochem Pharmacol 83:962-8. 2012
    ..Further variants were made to investigate the role of these amino acids. The results of this study may help to explain the mechanism of FMO function...
  6. pmc Characterization of human flavin-containing monooxygenase (FMO) 3 and FMO5 expressed as maltose-binding protein fusions
    Robert R Reddy
    Human BioMolecular Research Institute, 5310 Eastgate Mall, San Diego, CA 92121, USA
    Drug Metab Dispos 38:2239-45. 2010
    ..This report serves as the first direct comparison between Supersome FMOs and the corresponding MBP fusions and the first report of a detergent-based activation of k(cat)/K(m) that corresponds to changes in oligomerization...