Research Topics
Genomes and GenesSpecies | Philipp J KellerSummaryAffiliation: Howard Hughes Medical Institute Country: USA Publications
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Detail Information
Publications
Light sheet microscopy of living or cleared specimensPhilipp J Keller
Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA
Curr Opin Neurobiol 22:138-43. 2012..Here, we provide an overview of the history of light sheet microscopy, review the development of tissue clearing methods, and discuss recent technical breakthroughs that have the potential to influence the future direction of the field...
Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopyPhilipp J Keller
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
Nat Methods 7:637-42. 2010..We reconstructed cell positions over time from the Drosophila DSLM-SI data and created a fly digital embryo...
Reconstruction of zebrafish early embryonic development by scanned light sheet microscopyPhilipp J Keller
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Science 322:1065-9. 2008..We further derive a model of germ layer formation and show that the mesendoderm forms from one-third of the embryo's cells in a single event. Our digital embryos, with 55 million nucleus entries, are provided as a resource...
Nlcam modulates midline convergence during anterior neural plate morphogenesisKatherine E Brown
Developmental Biology, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Dev Biol 339:14-25. 2010....
Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopyRaju Tomer
Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA
Nat Methods 9:755-63. 2012..SiMView data sets provide quantitative morphological information even for fast global processes and enable accurate automated cell tracking in the entire early embryo...
Reconstructing embryonic developmentKhaled Khairy
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA
Genesis 49:488-513. 2011..We argue that the substantial increase in data complexity and size requires sophisticated new strategies to data analysis to exploit the enormous potential of these new resources...
Light sheet microscopy in cell biologyRaju Tomer
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA
Methods Mol Biol 931:123-37. 2013..We furthermore describe computational tools for basic image processing and data inspection...
Shedding light on the system: studying embryonic development with light sheet microscopyRaju Tomer
Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA
Curr Opin Genet Dev 21:558-65. 2011..We conclude with a discussion of promising future directions...
