Xiaoliang Sunney Xie

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Enzymatic signal amplification of molecular beacons for sensitive DNA detection
    Jianwei Jeffery Li
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Nucleic Acids Res 36:e36. 2008
  2. pmc RNAi screening for fat regulatory genes with SRS microscopy
    Meng C Wang
    Department of Molecular and Human Genetics and Huffington Center on Aging, Baylor College of Medicine, Houston, Texas, USA
    Nat Methods 8:135-8. 2011
  3. pmc Fluorogenic DNA sequencing in PDMS microreactors
    Peter A Sims
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA
    Nat Methods 8:575-80. 2011
  4. pmc Single-molecule imaging of transcription factor binding to DNA in live mammalian cells
    J Christof M Gebhardt
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA
    Nat Methods 10:421-6. 2013
  5. ncbi request reprint Single-molecule approach to molecular biology in living bacterial cells
    X Sunney Xie
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Annu Rev Biophys 37:417-44. 2008
  6. ncbi request reprint Living cells as test tubes
    X Sunney Xie
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 312:228-30. 2006
  7. pmc In vivo metabolic fingerprinting of neutral lipids with hyperspectral stimulated Raman scattering microscopy
    Dan Fu
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, United States
    J Am Chem Soc 136:8820-8. 2014
  8. pmc Hyperspectral imaging with stimulated Raman scattering by chirped femtosecond lasers
    Dan Fu
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Phys Chem B 117:4634-40. 2013
  9. pmc Coherent nonlinear optical imaging: beyond fluorescence microscopy
    Wei Min
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Annu Rev Phys Chem 62:507-30. 2011
  10. pmc Probing transcription factor dynamics at the single-molecule level in a living cell
    Johan Elf
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 316:1191-4. 2007

Collaborators

  • Gene Wei Li
  • Yong Yu
  • Ji Yu
  • Wei Min
  • Paul J Choi
  • Wei Yuan Yang
  • S C Kou
  • Xu Zhang
  • Giuseppe Lia
  • Martin Karplus
  • SUNNEY XIE
  • Eric O Potma
  • Dan Fu
  • Xiaolin Nan
  • Conor L Evans
  • Meng C Wang
  • Peter A Sims
  • Nir Friedman
  • Long Cai
  • J Christof M Gebhardt
  • Jianwei Jeffery Li
  • Johan Elf
  • Guobin Luo
  • Feruz Ganikhanov
  • Ji Xin Cheng
  • Erin Currie
  • Andrew Folick
  • Tsung Huang Tsai
  • Robert V Farese
  • Christian Freudiger
  • Tom Maniatis
  • Srinjan Basu
  • David M Suter
  • Ziqing W Zhao
  • Gary Holtom
  • Alec R Chapman
  • Rahul Roy
  • Christian W Freudiger
  • William J Greenleaf
  • Haifeng Duan
  • Gary Ruvkun
  • Yizhuo Chu
  • Benjamin Yi Hung Lee
  • Brian G Saar
  • John Paul Pezacki
  • Albert Stolow
  • Angela M Tonary
  • Ioan Andricioaei
  • Mehron Puoris'haag
  • Daniel Côté
  • Charles P Lin
  • Peng Chen
  • David A Weitz
  • Sophie Pautot

Detail Information

Publications25

  1. pmc Enzymatic signal amplification of molecular beacons for sensitive DNA detection
    Jianwei Jeffery Li
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Nucleic Acids Res 36:e36. 2008
    ..Moreover, the extended version provides one additional level of signal amplification, bringing the detection limit down to tens of femtomolar, nearly five orders of magnitude lower than that of conventional hybridization assay...
  2. pmc RNAi screening for fat regulatory genes with SRS microscopy
    Meng C Wang
    Department of Molecular and Human Genetics and Huffington Center on Aging, Baylor College of Medicine, Houston, Texas, USA
    Nat Methods 8:135-8. 2011
    ..Our screening yielded eight new genetic regulators of fat storage...
  3. pmc Fluorogenic DNA sequencing in PDMS microreactors
    Peter A Sims
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA
    Nat Methods 8:575-80. 2011
    ....
  4. pmc Single-molecule imaging of transcription factor binding to DNA in live mammalian cells
    J Christof M Gebhardt
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA
    Nat Methods 10:421-6. 2013
    ..Our single-molecule measurements and statistical analysis revealed dynamic properties of transcription factors...
  5. ncbi request reprint Single-molecule approach to molecular biology in living bacterial cells
    X Sunney Xie
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Annu Rev Biophys 37:417-44. 2008
    ..Here we review the key methods of single-molecule detection and highlight numerous examples to illustrate how these experiments are contributing to the quantitative understanding of the fundamental processes in a living cell...
  6. ncbi request reprint Living cells as test tubes
    X Sunney Xie
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 312:228-30. 2006
    ..Here, we describe the application of these techniques in studying gene expression, active transport, and lipid metabolism...
  7. pmc In vivo metabolic fingerprinting of neutral lipids with hyperspectral stimulated Raman scattering microscopy
    Dan Fu
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, United States
    J Am Chem Soc 136:8820-8. 2014
    ..Our findings provide new approaches for metabolic tracing of neutral lipids and their precursors in living cells and organisms, and could potentially serve as a general approach for metabolic fingerprinting of other metabolites. ..
  8. pmc Hyperspectral imaging with stimulated Raman scattering by chirped femtosecond lasers
    Dan Fu
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Phys Chem B 117:4634-40. 2013
    ..The combination of fast spectroscopy and label-free chemical imaging will enable new applications in studying biological systems and material systems...
  9. pmc Coherent nonlinear optical imaging: beyond fluorescence microscopy
    Wei Min
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Annu Rev Phys Chem 62:507-30. 2011
    ..Here we review the underlying principles and excitation and detection schemes, as well as exemplary biomedical applications of this emerging class of molecular imaging techniques...
  10. pmc Probing transcription factor dynamics at the single-molecule level in a living cell
    Johan Elf
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 316:1191-4. 2007
    ..The methods and findings can be generalized to other nucleic acid binding proteins...
  11. ncbi request reprint Probing gene expression in live cells, one protein molecule at a time
    Ji Yu
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 311:1600-3. 2006
    ..The quantitative study of low-level gene expression demonstrates the potential of single-molecule experiments in elucidating the workings of fundamental biological processes in living cells...
  12. pmc A stochastic single-molecule event triggers phenotype switching of a bacterial cell
    Paul J Choi
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 322:442-6. 2008
    ..Hence, a stochastic single-molecule event determines a cell's phenotype...
  13. ncbi request reprint Linking stochastic dynamics to population distribution: an analytical framework of gene expression
    Nir Friedman
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Phys Rev Lett 97:168302. 2006
    ....
  14. ncbi request reprint Intracellular imaging of HCV RNA and cellular lipids by using simultaneous two-photon fluorescence and coherent anti-Stokes Raman scattering microscopies
    Xiaolin Nan
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    Chembiochem 7:1895-7. 2006
  15. ncbi request reprint High-sensitivity vibrational imaging with frequency modulation coherent anti-Stokes Raman scattering (FM CARS) microscopy
    Feruz Ganikhanov
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
    Opt Lett 31:1872-4. 2006
    ..This scheme efficiently suppresses the nonresonant background and allows for the detection of far fewer vibrational oscillators than possible through existing CARS microscopy methods...
  16. ncbi request reprint Coherent anti-stokes raman scattering spectral interferometry: determination of the real and imaginary components of nonlinear susceptibility chi(3) for vibrational microscopy
    Conor L Evans
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
    Opt Lett 29:2923-5. 2004
    ..Heterodyne detection offers potential for signal amplification and enhanced sensitivity for CARS microscopy...
  17. ncbi request reprint Direct visualization of lipid phase segregation in single lipid bilayers with coherent anti-Stokes Raman scattering microscopy
    Eric O Potma
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
    Chemphyschem 6:77-9. 2005
  18. ncbi request reprint Observation of a power-law memory kernel for fluctuations within a single protein molecule
    Wei Min
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Phys Rev Lett 94:198302. 2005
    ..K(t) is a power-law decay, t(-0.51 +/- 0.07) in a broad range of time scales (10(-3)-10 s). Such a long-time memory effect could have implications for protein functions...
  19. pmc Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy
    Conor L Evans
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 102:16807-12. 2005
    ....
  20. ncbi request reprint Observation of individual microtubule motor steps in living cells with endocytosed quantum dots
    Xiaolin Nan
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Phys Chem B 109:24220-4. 2005
    ..This work demonstrates the ability of the QD assay to probe the operation of motor proteins at the molecular level in living cells under physiological conditions...
  21. ncbi request reprint Heterodyne coherent anti-Stokes Raman scattering (CARS) imaging
    Eric O Potma
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Opt Lett 31:241-3. 2006
    ..We show that heterodyne CARS microscopy permits the detection of weak vibrational resonances that are otherwise overshadowed by the strong interference of the nonresonant background...
  22. ncbi request reprint Stochastic protein expression in individual cells at the single molecule level
    Long Cai
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
    Nature 440:358-62. 2006
    ..This microfluidic single cell assay opens up possibilities for system-wide characterization of the expression of these low copy number proteins...
  23. pmc Nonperturbative chemical imaging of organelle transport in living cells with coherent anti-stokes Raman scattering microscopy
    Xiaolin Nan
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA
    Biophys J 91:728-35. 2006
    ..These observations demonstrate CARS microscopy as a powerful noninvasive imaging tool for studying dynamic processes in living cells...
  24. ncbi request reprint Dynamic distance disorder in proteins is caused by trapping
    Guobin Luo
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    J Phys Chem B 110:9363-7. 2006
    ....
  25. pmc Ordering of water molecules between phospholipid bilayers visualized by coherent anti-Stokes Raman scattering microscopy
    Ji Xin Cheng
    Department of Chemistry and Chemical Biology, Division of Engineering and Applied Sciences, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 100:9826-30. 2003
    ..The spectral profile for the inter-lamellar water shows that the water molecules bound to the bilayer surface are less hydrogen-bonded and exhibit a higher vibrational frequency than bulk water...

Research Grants17

  1. Real-time single-molecule nucleic acid sequencing with fluorogenic nucleotides
    Xiaoliang Sunney Xie; Fiscal Year: 2010
    ..This ability to economically sequence full genomes will usher in a new era of personalized medicine. ..
  2. SINGLE-MOLECULE ENZYMATIC DYNAMICS OF FLAVOPROTEINS
    SUNNEY XIE; Fiscal Year: 2000
    ..This work and its variations will significantly enhance the knowledge of how protein dynamics influence enzymatic functions. ..
  3. Imaging the Invisible with No Labels: A Major Opportunity in Biology and Medicine
    Xiaoliang Sunney Xie; Fiscal Year: 2010
    ....
  4. Imaging the Invisible with No Labels: A Major Opportunity in Biology and Medicine
    Xiaoliang Sunney Xie; Fiscal Year: 2009
    ....
  5. Real-time single-molecule nucleic acid sequencing with fluorogenic nucleotides
    Xiaoliang Sunney Xie; Fiscal Year: 2009
    ..This ability to economically sequence full genomes will usher in a new era of personalized medicine. ..
  6. 2006 Single Molecule Approaches to Biology
    SUNNEY XIE; Fiscal Year: 2006
    ....
  7. Real-time observation of gene expression in live cells
    SUNNEY XIE; Fiscal Year: 2005
    ..The new approach is likely to have a broad impact on biomedical research and lead to unexpected discoveries. ..
  8. SINGLE-MOLECULE ENZYMATIC DYNAMICS OF FLAVOPROTEINS
    SUNNEY XIE; Fiscal Year: 2003
    ..This work and its variations will significantly enhance the knowledge of how protein dynamics influence enzymatic functions. ..
  9. COHERENT ANTI-STOKES RAMAN SCATTERING MICROSCOPY
    SUNNEY XIE; Fiscal Year: 2004
    ..This proposal focuses on the development of a new methodology to tackle compelling problems in biology. Although we give many examples of anticipated applications, the new methodology will likely to lead to exciting unexpected results. ..
  10. COHERENT ANTI-STOKES RAMAN SCATTERING MICROSCOPY
    SUNNEY XIE; Fiscal Year: 2003
    ..This proposal focuses on the development of a new methodology to tackle compelling problems in biology. Although we give many examples of anticipated applications, the new methodology will likely to lead to exciting unexpected results. ..
  11. SINGLE-MOLECULE ENZYMATIC DYNAMICS OF FLAVOPROTEINS
    SUNNEY XIE; Fiscal Year: 2003
    ..This work and its variations will significantly enhance the knowledge of how protein dynamics influence enzymatic functions. ..
  12. COHERENT ANTI-STOKES RAMAN SCATTERING MICROSCOPY
    SUNNEY XIE; Fiscal Year: 2002
    ..This proposal focuses on the development of a new methodology to tackle compelling problems in biology. Although we give many examples of anticipated applications, the new methodology will likely to lead to exciting unexpected results. ..
  13. SINGLE-MOLECULE ENZYMATIC DYNAMICS OF FLAVOPROTEINS
    SUNNEY XIE; Fiscal Year: 2002
    ..This work and its variations will significantly enhance the knowledge of how protein dynamics influence enzymatic functions. ..
  14. SINGLE-MOLECULE ENZYMATIC DYNAMICS OF FLAVOPROTEINS
    SUNNEY XIE; Fiscal Year: 2001
    ..This work and its variations will significantly enhance the knowledge of how protein dynamics influence enzymatic functions. ..
  15. SINGLE-MOLECULE ENZYMATIC DYNAMICS OF FLAVOPROTEINS
    SUNNEY XIE; Fiscal Year: 2001
    ..This work and its variations will significantly enhance the knowledge of how protein dynamics influence enzymatic functions. ..
  16. COHERENT ANTI-STOKES RAMAN SCATTERING MICROSCOPY
    SUNNEY XIE; Fiscal Year: 2001
    ..This proposal focuses on the development of a new methodology to tackle compelling problems in biology. Although we give many examples of anticipated applications, the new methodology will likely to lead to exciting unexpected results. ..
  17. Real-time single-molecule nucleic acid sequencing with fluorogenic nucleotides
    Xiaoliang Sunney Xie; Fiscal Year: 2010
    ..We also propose the integration of a massively parallel single molecule fluorogenic sequencer with microfluidic devices that process and deliver genetic material from a single cell. ..