Harris H Wang

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi Genome-scale promoter engineering by coselection MAGE
    Harris H Wang
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA
    Nat Methods 9:591-3. 2012
  2. ncbi Improving lambda red genome engineering in Escherichia coli via rational removal of endogenous nucleases
    Joshua A Mosberg
    Program in Chemical Biology, Harvard University, Cambridge, Massachusetts, United States of America
    PLoS ONE 7:e44638. 2012
  3. ncbi Precise manipulation of chromosomes in vivo enables genome-wide codon replacement
    Farren J Isaacs
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 333:348-53. 2011
  4. ncbi Modified bases enable high-efficiency oligonucleotide-mediated allelic replacement via mismatch repair evasion
    Harris H Wang
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Department of Genetics, Harvard Medical School, Harvard University, Boston, MA 02115, USA
    Nucleic Acids Res 39:7336-47. 2011
  5. ncbi Synthetic genomes for synthetic biology
    Harris H Wang
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    J Mol Cell Biol 2:178-9. 2010
  6. ncbi Multiplexed genome engineering and genotyping methods applications for synthetic biology and metabolic engineering
    Harris H Wang
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
    Methods Enzymol 498:409-26. 2011
  7. ncbi Programming cells by multiplex genome engineering and accelerated evolution
    Harris H Wang
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 460:894-8. 2009

Collaborators

Detail Information

Publications7

  1. ncbi Genome-scale promoter engineering by coselection MAGE
    Harris H Wang
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA
    Nat Methods 9:591-3. 2012
    ..Promoter libraries can be quickly generated to study gain-of-function epistatic interactions in gene networks...
  2. ncbi Improving lambda red genome engineering in Escherichia coli via rational removal of endogenous nucleases
    Joshua A Mosberg
    Program in Chemical Biology, Harvard University, Cambridge, Massachusetts, United States of America
    PLoS ONE 7:e44638. 2012
    ..The results described in this work provide further mechanistic insight into recombineering, and substantially improve recombineering performance...
  3. ncbi Precise manipulation of chromosomes in vivo enables genome-wide codon replacement
    Farren J Isaacs
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 333:348-53. 2011
    ..Our methods treat the chromosome as both an editable and an evolvable template, permitting the exploration of vast genetic landscapes...
  4. ncbi Modified bases enable high-efficiency oligonucleotide-mediated allelic replacement via mismatch repair evasion
    Harris H Wang
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Department of Genetics, Harvard Medical School, Harvard University, Boston, MA 02115, USA
    Nucleic Acids Res 39:7336-47. 2011
    ..These results further elucidate the mechanism of oligo-mediated allelic replacement (OMAR) and enable improved methodologies for efficient, large-scale engineering of genomes...
  5. ncbi Synthetic genomes for synthetic biology
    Harris H Wang
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    J Mol Cell Biol 2:178-9. 2010
    ..This achievement marks a new frontier in synthetic biology to design and create genomes for organisms with few genetic tools and for applications in areas of energy, health care and the environment...
  6. ncbi Multiplexed genome engineering and genotyping methods applications for synthetic biology and metabolic engineering
    Harris H Wang
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
    Methods Enzymol 498:409-26. 2011
    ..Here, we discuss ways to utilize these multiplexed genome engineering methods, with special emphasis on experimental design and implementation...
  7. ncbi Programming cells by multiplex genome engineering and accelerated evolution
    Harris H Wang
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 460:894-8. 2009
    ..Our multiplex approach embraces engineering in the context of evolution by expediting the design and evolution of organisms with new and improved properties...