Harris H Wang

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. doi request reprint Synthetic genomes for synthetic biology
    Harris H Wang
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    J Mol Cell Biol 2:178-9. 2010
  2. doi request reprint Programming cells by multiplex genome engineering and accelerated evolution
    Harris H Wang
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 460:894-8. 2009
  3. pmc Modified bases enable high-efficiency oligonucleotide-mediated allelic replacement via mismatch repair evasion
    Harris H Wang
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Department of Genetics, Harvard Medical School, Harvard University, Boston, MA 02115, USA
    Nucleic Acids Res 39:7336-47. 2011
  4. doi request reprint Genomically recoded organisms expand biological functions
    Marc J Lajoie
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 342:357-60. 2013
  5. doi request reprint Precise manipulation of chromosomes in vivo enables genome-wide codon replacement
    Farren J Isaacs
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 333:348-53. 2011
  6. pmc Genome-scale promoter engineering by coselection MAGE
    Harris H Wang
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA
    Nat Methods 9:591-3. 2012
  7. pmc Syntrophic exchange in synthetic microbial communities
    Michael T Mee
    Department of Biomedical Engineering, Boston University, Boston, MA 02215 Department of Genetics, Harvard Medical School, Boston, MA 02115
    Proc Natl Acad Sci U S A 111:E2149-56. 2014
  8. pmc Genome-scale engineering for systems and synthetic biology
    Kevin M Esvelt
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA
    Mol Syst Biol 9:641. 2013
  9. doi request reprint Multiplexed genome engineering and genotyping methods applications for synthetic biology and metabolic engineering
    Harris H Wang
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
    Methods Enzymol 498:409-26. 2011
  10. doi request reprint Improving microbial fitness in the mammalian gut by in vivo temporal functional metagenomics
    Stephanie J Yaung
    Program in Medical Engineering and Medical Physics, Harvard MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA Department of Genetics, Harvard Medical School, Boston, MA, USA Wyss Institute for Biologically inspired Engineering, Harvard University, Boston, MA, USA
    Mol Syst Biol 11:788. 2015

Collaborators

Detail Information

Publications11

  1. doi request reprint Synthetic genomes for synthetic biology
    Harris H Wang
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    J Mol Cell Biol 2:178-9. 2010
    ..This achievement marks a new frontier in synthetic biology to design and create genomes for organisms with few genetic tools and for applications in areas of energy, health care and the environment...
  2. doi request reprint Programming cells by multiplex genome engineering and accelerated evolution
    Harris H Wang
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 460:894-8. 2009
    ..Our multiplex approach embraces engineering in the context of evolution by expediting the design and evolution of organisms with new and improved properties...
  3. pmc Modified bases enable high-efficiency oligonucleotide-mediated allelic replacement via mismatch repair evasion
    Harris H Wang
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Department of Genetics, Harvard Medical School, Harvard University, Boston, MA 02115, USA
    Nucleic Acids Res 39:7336-47. 2011
    ..These results further elucidate the mechanism of oligo-mediated allelic replacement (OMAR) and enable improved methodologies for efficient, large-scale engineering of genomes...
  4. doi request reprint Genomically recoded organisms expand biological functions
    Marc J Lajoie
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 342:357-60. 2013
    ..The GRO also exhibited increased resistance to T7 bacteriophage, demonstrating that new genetic codes could enable increased viral resistance. ..
  5. doi request reprint Precise manipulation of chromosomes in vivo enables genome-wide codon replacement
    Farren J Isaacs
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 333:348-53. 2011
    ..Our methods treat the chromosome as both an editable and an evolvable template, permitting the exploration of vast genetic landscapes...
  6. pmc Genome-scale promoter engineering by coselection MAGE
    Harris H Wang
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA
    Nat Methods 9:591-3. 2012
    ..Promoter libraries can be quickly generated to study gain-of-function epistatic interactions in gene networks...
  7. pmc Syntrophic exchange in synthetic microbial communities
    Michael T Mee
    Department of Biomedical Engineering, Boston University, Boston, MA 02215 Department of Genetics, Harvard Medical School, Boston, MA 02115
    Proc Natl Acad Sci U S A 111:E2149-56. 2014
    ..This work sets the foundation for future endeavors to resolve key questions in microbial ecology and evolution, and presents a platform to develop better and more robust engineered synthetic communities for industrial biotechnology. ..
  8. pmc Genome-scale engineering for systems and synthetic biology
    Kevin M Esvelt
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA
    Mol Syst Biol 9:641. 2013
    ....
  9. doi request reprint Multiplexed genome engineering and genotyping methods applications for synthetic biology and metabolic engineering
    Harris H Wang
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
    Methods Enzymol 498:409-26. 2011
    ..Here, we discuss ways to utilize these multiplexed genome engineering methods, with special emphasis on experimental design and implementation...
  10. doi request reprint Improving microbial fitness in the mammalian gut by in vivo temporal functional metagenomics
    Stephanie J Yaung
    Program in Medical Engineering and Medical Physics, Harvard MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA Department of Genetics, Harvard Medical School, Boston, MA, USA Wyss Institute for Biologically inspired Engineering, Harvard University, Boston, MA, USA
    Mol Syst Biol 11:788. 2015
    ..coli genome driving increased galactose utilization. Our findings highlight the utility of functional metagenomics for engineering commensal bacteria with improved properties, including expanded colonization capabilities in vivo. ..
  11. pmc Yeast oligo-mediated genome engineering (YOGE)
    James E Dicarlo
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, United States
    ACS Synth Biol 2:741-9. 2013
    ..YOGE cycling alone or in combination with phenotypic selections or endonuclease-based negative genotypic selections can be used to generate modified alleles easily in yeast populations with high frequencies. ..