Bakhos A Tannous

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Secreted Gaussia luciferase as a biomarker for monitoring tumor progression and treatment response of systemic metastases
    Euiheon Chung
    Edwin L Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
    PLoS ONE 4:e8316. 2009
  2. pmc A highly sensitive assay for monitoring the secretory pathway and ER stress
    Christian E Badr
    Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital, Boston, Massachusetts, United States of America Program in Neuroscience, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 2:e571. 2007
  3. pmc Triple bioluminescence imaging for in vivo monitoring of cellular processes
    Casey A Maguire
    1 Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, Massachusetts, USA 2 Program in Neuroscience, Harvard Medical School, Boston, Massachusetts, USA
    Mol Ther Nucleic Acids 2:e99. 2013
  4. pmc Imaging gene delivery in a mouse model of congenital neuronal ceroid lipofuscinosis
    L S Pike
    Department of Neurology, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Boston, MA 02129, USA
    Gene Ther 18:1173-8. 2011
  5. pmc Secreted blood reporters: insights and applications
    Bakhos A Tannous
    Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, MA 02129, USA
    Biotechnol Adv 29:997-1003. 2011
  6. pmc Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo
    Bakhos A Tannous
    Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nat Protoc 4:582-91. 2009
  7. pmc Mutant sodium channel for tumor therapy
    Bakhos A Tannous
    Department of Neurology, Molecular Neurogenetics Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02129, USA
    Mol Ther 17:810-9. 2009
  8. ncbi request reprint Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo
    Bakhos A Tannous
    Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Mol Ther 11:435-43. 2005
  9. pmc A secreted luciferase for ex vivo monitoring of in vivo processes
    Thomas Wurdinger
    Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, 149 13th St, Charlestown, Massachusetts 02129, USA
    Nat Methods 5:171-3. 2008
  10. pmc miR-296 regulates growth factor receptor overexpression in angiogenic endothelial cells
    Thomas Wurdinger
    Department of Neurology, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 14:382-93. 2008

Collaborators

Detail Information

Publications42

  1. pmc Secreted Gaussia luciferase as a biomarker for monitoring tumor progression and treatment response of systemic metastases
    Euiheon Chung
    Edwin L Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
    PLoS ONE 4:e8316. 2009
    ..To this end, we engineered tumor cells to express a naturally secreted Gaussia luciferase (Gluc), and investigated its use as a circulating biomarker for monitoring viable metastatic or primary tumor growth and their treatment responses...
  2. pmc A highly sensitive assay for monitoring the secretory pathway and ER stress
    Christian E Badr
    Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital, Boston, Massachusetts, United States of America Program in Neuroscience, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 2:e571. 2007
    ..We have developed a highly sensitive assay to monitor processing of proteins through the secretory pathway and endoplasmic reticulum (ER) stress in real-time based on the naturally secreted Gaussia luciferase (Gluc)...
  3. pmc Triple bioluminescence imaging for in vivo monitoring of cellular processes
    Casey A Maguire
    1 Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, Massachusetts, USA 2 Program in Neuroscience, Harvard Medical School, Boston, Massachusetts, USA
    Mol Ther Nucleic Acids 2:e99. 2013
    ..Molecular Therapy - Nucleic Acids (2013) 2, e99; doi:10.1038/mtna.2013.25; published online 18 June 2013...
  4. pmc Imaging gene delivery in a mouse model of congenital neuronal ceroid lipofuscinosis
    L S Pike
    Department of Neurology, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Boston, MA 02129, USA
    Gene Ther 18:1173-8. 2011
    ..These studies support the potential effectiveness and imaging evaluation of enzyme replacement therapy to the brain and other organs in CathD null mice via AAV-mediated gene delivery in neonatal animals...
  5. pmc Secreted blood reporters: insights and applications
    Bakhos A Tannous
    Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, MA 02129, USA
    Biotechnol Adv 29:997-1003. 2011
    ..We also comment on other recently discovered secreted luciferases and their potential use as blood reporters for multiplexing applications...
  6. pmc Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo
    Bakhos A Tannous
    Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nat Protoc 4:582-91. 2009
    ..Additionally, the Gluc signal can be detected in urine and the signal can be localized in animals using in vivo bioluminescence imaging...
  7. pmc Mutant sodium channel for tumor therapy
    Bakhos A Tannous
    Department of Neurology, Molecular Neurogenetics Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02129, USA
    Mol Ther 17:810-9. 2009
    ..The advantage of this direct mode of tumor therapy is that all types of tumor cells become susceptible and death is rapid with no time for the tumor cells to become resistant...
  8. ncbi request reprint Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo
    Bakhos A Tannous
    Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Mol Ther 11:435-43. 2005
    ....
  9. pmc A secreted luciferase for ex vivo monitoring of in vivo processes
    Thomas Wurdinger
    Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, 149 13th St, Charlestown, Massachusetts 02129, USA
    Nat Methods 5:171-3. 2008
    ....
  10. pmc miR-296 regulates growth factor receptor overexpression in angiogenic endothelial cells
    Thomas Wurdinger
    Department of Neurology, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 14:382-93. 2008
    ..Furthermore, inhibition of miR-296 with antagomirs reduces angiogenesis in tumor xenografts in vivo...
  11. ncbi request reprint Gaussia luciferase-based mycoplasma detection assay in mammalian cell culture
    M Hannah Degeling
    Experimental Therapeutics and Molecular Imaging Laboratory, Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA
    Methods Mol Biol 1098:47-55. 2014
    ..In this chapter, we describe a simple and sensitive mycoplasma detection assay based on the bioluminescent properties of the Gaussia luciferase reporter. ..
  12. pmc Single reporter for targeted multimodal in vivo imaging
    Johanna M Niers
    Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, United States
    J Am Chem Soc 134:5149-56. 2012
    ....
  13. pmc Human glioma growth is controlled by microRNA-10b
    Galina Gabriely
    Department of Neurology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cancer Res 71:3563-72. 2011
    ..Altogether, our experiments validate an important role of miR-10b in gliomagenesis, reveal a novel mechanism of miR-10b-mediated regulation, and suggest the possibility of its future use as a therapeutic target in gliomas...
  14. pmc Gaussia luciferase variant for high-throughput functional screening applications
    Casey A Maguire
    Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, Boston Massachusetts 02115, USA
    Anal Chem 81:7102-6. 2009
    ..We isolated a Gluc mutant that catalyzes enhanced light stability in the presence of a detergent, in combination with high sensitivity, making it an attractive luciferase for high-throughput functional screening applications...
  15. pmc Combined targeting of HER2 and VEGFR2 for effective treatment of HER2-amplified breast cancer brain metastases
    David P Kodack
    Edwin L Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 109:E3119-27. 2012
    ..These findings support the clinical development of this three-drug regimen for the treatment of HER2-amplified breast cancer brain metastases...
  16. pmc Microvesicle-associated AAV vector as a novel gene delivery system
    Casey A Maguire
    Department of Neurology, Massachusetts General Hospital, and Neuroscience Program, Harvard Medical School, Boston, Massachusetts, USA
    Mol Ther 20:960-71. 2012
    ..Finally, we show that vexosomes bound to magnetic beads can be attracted to a magnetized area in cultured cells. Vexosomes represent a unique entity which offers a promising strategy to improve gene delivery...
  17. pmc Multimodal in vivo imaging and blood monitoring of intrinsic and extrinsic apoptosis
    Johanna M Niers
    Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Charlestown, Massachusetts, USA
    Mol Ther 19:1090-6. 2011
    ..This reporter provides a valuable tool for imaging and real-time monitoring of apoptosis and is compatible with high-throughput functional screening application in cultured cells and animal models...
  18. pmc Targeting cancer cells with the natural compound obtusaquinone
    Christian E Badr
    Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA
    J Natl Cancer Inst 105:643-53. 2013
    ..Cancer therapeutics exploiting such biochemical changes by increasing reactive oxygen species (ROS) production or decreasing intracellular ROS scavengers could provide a powerful treatment strategy...
  19. pmc Real-time monitoring of nuclear factor kappaB activity in cultured cells and in animal models
    Christian E Badr
    Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Charlestown, 02129, USA
    Mol Imaging 8:278-90. 2009
    ..This NF-kappaB reporter system provides a powerful tool for monitoring NF-kappaB activity in real time in vitro and in vivo...
  20. pmc Effects of the selective MPS1 inhibitor MPS1-IN-3 on glioblastoma sensitivity to antimitotic drugs
    Bakhos A Tannous
    Neuroscience Center and Molecular Neurogenetics Unit, Departments of Neurology, Harvard Medical School, Boston, MA, USA
    J Natl Cancer Inst 105:1322-31. 2013
    ..During the mitotic agent-induced arrest, glioblastoma cells are able to perform damage-control and self-repair to continue proliferation. Monopolar spindle 1 (MPS1/TTK) is a checkpoint kinase and a gatekeeper of the mitotic arrest...
  21. pmc Performance of the red-shifted fluorescent proteins in deep-tissue molecular imaging applications
    Nikolaos C Deliolanis
    Massachusetts General Hospital and Harvard Medical School, Center for Molecular Imaging Research, Laboratory for Bio optics and Molecular Imaging, Charlestown, Massachusetts 02139, USA
    J Biomed Opt 13:044008. 2008
    ....
  22. pmc Glioma angiogenesis: Towards novel RNA therapeutics
    Thomas Wurdinger
    Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, USA
    Cell Adh Migr 3:230-5. 2009
    ..In this review we will discuss the emerging technologies in anti-angiogenesis emphasizing on RNA-based therapeutics...
  23. ncbi request reprint Simultaneous in vivo monitoring of regulatory and effector T lymphocytes using secreted Gaussia luciferase, Firefly luciferase, and secreted alkaline phosphatase
    Grant K Lewandrowski
    Experimental Therapeutics and Molecular Imaging Laboratory, Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA
    Methods Mol Biol 1098:211-27. 2014
    ..Furthermore, this novel technique has applications not only in autoimmunity and alloimmunity, but also in the wider field of immunology. ..
  24. pmc Dynamic biodistribution of extracellular vesicles in vivo using a multimodal imaging reporter
    Charles P Lai
    Department of Neurology, Department of Radiology, Center for Systems Biology, and Center for Molecular Imaging Research, Massachusetts General Hospital, Charlestown, Massachusetts 02129, United States
    ACS Nano 8:483-94. 2014
    ..Altogether, we show the EVs are dynamically processed in vivo with accurate spatiotemporal resolution and target a number of normal organs as well as tumors with implications for disease pathology and therapeutic design. ..
  25. pmc Directed molecular evolution reveals Gaussia luciferase variants with enhanced light output stability
    M Hannah Degeling
    Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Charlestown, MA 02129, United States
    Anal Chem 85:3006-12. 2013
    ..These results indicate that molecular evolution could be used to modulate Gluc bioluminescence reaction characteristics...
  26. pmc Sensitive assay for mycoplasma detection in mammalian cell culture
    M Hannah Degeling
    Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Harvard Medical School, Boston, Massachusetts 02114, USA
    Anal Chem 84:4227-32. 2012
    ..The Gaussia luciferase mycosensor assay provides an easy tool to monitor mammalian cell contaminants in a high-throughput fashion...
  27. pmc Downregulated microRNA-200a in meningiomas promotes tumor growth by reducing E-cadherin and activating the Wnt/beta-catenin signaling pathway
    Okay Saydam
    Department of Neurology, Massachusetts General Hospital, and Neuroscience Program, Harvard Medical School, Boston, Massachusetts 02129, USA
    Mol Cell Biol 29:5923-40. 2009
    ....
  28. ncbi request reprint Noninvasive in vivo monitoring of extracellular vesicles
    Charles P Lai
    Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA
    Methods Mol Biol 1098:249-58. 2014
    ..We here describe a strategy to label EVs with Gaussia luciferase for noninvasive bioluminescence imaging and monitoring of systemically administered EVs in vivo. ..
  29. pmc Multiplex blood reporters for simultaneous monitoring of cellular processes
    M Sarah S Bovenberg
    Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, Massachusetts 02129, United States
    Anal Chem 85:10205-10. 2013
    ..This system could be extended to any field to detect multiple processes in the same biological system and is amenable for high-throughput screening to find drugs that affect multiple cellular populations/phenomena simultaneously. ..
  30. pmc siRNA knock-down of mutant torsinA restores processing through secretory pathway in DYT1 dystonia cells
    Jeffrey W Hewett
    Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Hum Mol Genet 17:1436-45. 2008
    ..The ability of allele-specific siRNA for torsinADeltaE to normalize secretory function in DYT1 patient cells supports its potential role as a therapeutic agent in early onset torsion dystonia...
  31. pmc Cell-based immunotherapy against gliomas: from bench to bedside
    M Sarah S Bovenberg
    Department of Neurology, Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Massachusetts General Hospital, Boston, Massachusetts, USA
    Mol Ther 21:1297-305. 2013
    ..In this review, we discuss advances in active, passive, and vaccine-based immunotherapeutic strategies for gliomas both at the bench and in the clinic. ..
  32. pmc Lanatoside C sensitizes glioblastoma cells to tumor necrosis factor-related apoptosis-inducing ligand and induces an alternative cell death pathway
    Christian E Badr
    Neuroscience Center and Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA
    Neuro Oncol 13:1213-24. 2011
    ..Activation of such mechanism may be a useful strategy to counter resistance of cancer cells to apoptosis...
  33. pmc Functional drug screening assay reveals potential glioma therapeutics
    Christian E Badr
    Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, USA
    Assay Drug Dev Technol 9:281-9. 2011
    ..In conclusion, the Gluc secretion assay is a robust tool for functional drug screening and can be applied to many different fields including cancer...
  34. ncbi request reprint Codon-optimized Luciola italica luciferase variants for mammalian gene expression in culture and in vivo
    Casey A Maguire
    Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
    Mol Imaging 11:13-21. 2012
    ..Overall, our characterization of these codon-optimized luciferases lays the groundwork for their further use as bioluminescent reporters in mammalian cells...
  35. pmc Enhanced Gaussia luciferase blood assay for monitoring of in vivo biological processes
    M Sarah S Bovenberg
    Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, United States
    Anal Chem 84:1189-92. 2012
    ..This assay showed to be over 1 order of magnitude more sensitive in detecting Gluc in the blood as compared to the direct Gluc blood assay enhancing ex vivo monitoring of biological processes...
  36. ncbi request reprint Metabolic biotinylation of cell surface receptors for in vivo imaging
    Bakhos A Tannous
    Center for Molecular Imaging Research, Massachusetts General Hospital East, Building 149, 13th Street, Charlestown, Massachusetts 02129, USA
    Nat Methods 3:391-6. 2006
    ..This BAP-TM allows noninvasive real-time imaging of any cell type transduced to express this reporter protein in culture or in vivo...
  37. pmc Enhanced in vivo imaging of metabolically biotinylated cell surface reporters
    Johanna M Niers
    Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    Anal Chem 83:994-9. 2011
    ..These findings have practical applications in designing more efficient targeting and imaging strategies...
  38. doi request reprint Measurement of fluoride-induced endoplasmic reticulum stress using Gaussia luciferase
    Ramaswamy Sharma
    Department of Cytokine Biology, Forsyth Institute, Cambridge, Massachusetts, USA
    Methods Enzymol 491:111-25. 2011
    ..Since Gluc can be measured in a noninvasive manner, it has been used in several in vitro and in vivo applications. In this chapter, we detail our methodology for using Gluc to monitor fluoride-induced ER stress...
  39. doi request reprint Advances in stem cell therapy against gliomas
    M Sarah S Bovenberg
    Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, and Program in Neuroscience, Harvard Medical School, Boston, MA 02129, USA
    Trends Mol Med 19:281-91. 2013
    ..In this review, we discuss current trends and the latest developments in stem cell therapy against malignant gliomas from both the experimental laboratory and the clinic...
  40. doi request reprint Bioluminescence imaging: progress and applications
    Christian E Badr
    Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA
    Trends Biotechnol 29:624-33. 2011
    ..In this review, we discuss current trends in bioluminescence and its application in different fields with an emphasis on cancer research...
  41. pmc Pharmacokinetics of natural and engineered secreted factors delivered by mesenchymal stromal cells
    Jessica S Elman
    Department of Surgery, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School and the Shriners Hospital for Children, Boston, Massachusetts, United States of America
    PLoS ONE 9:e89882. 2014
    ..We assert that a combined consideration of cell delivery strategies and molecular pharmacokinetics can provide a more predictive model for outcomes of MSC transplantation and potentially other transient cell therapeutics. ..
  42. pmc T7 RNA polymerase as a self-replicating label for antigen quantification
    Bakhos A Tannous
    Department of Chemistry and Biochemistry, University of Windsor, Ontario N9B 3P4, Canada
    Nucleic Acids Res 30:e140. 2002
    ..Furthermore, the T7RP is biotinylated, complexed with streptavidin and used for antigen quantification in a microtiter well-based assay with high sensitivity and reproducibility...