Research Topics
Genomes and Genes | Bakhos A TannousSummaryAffiliation: Harvard University Country: USA Publications
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Publications
Mutant sodium channel for tumor therapyBakhos A Tannous
Department of Neurology, Molecular Neurogenetics Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02129, USA
Mol Ther 17:810-9. 2009..The advantage of this direct mode of tumor therapy is that all types of tumor cells become susceptible and death is rapid with no time for the tumor cells to become resistant...
Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivoBakhos A Tannous
Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital, Charlestown, MA 02129, USA
Mol Ther 11:435-43. 2005....- Secreted blood reporters: insights and applicationsBakhos A Tannous
Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, MA 02129, USA
Biotechnol Adv 29:997-1003. 2011..We also comment on other recently discovered secreted luciferases and their potential use as blood reporters for multiplexing applications... - Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivoBakhos A Tannous
Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, Boston, Massachusetts 02114, USA
Nat Protoc 4:582-91. 2009..Additionally, the Gluc signal can be detected in urine and the signal can be localized in animals using in vivo bioluminescence imaging... - Single reporter for targeted multimodal in vivo imagingJohanna M Niers
Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, United States
J Am Chem Soc 134:5149-56. 2012.... - Human glioma growth is controlled by microRNA-10bGalina Gabriely
Department of Neurology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
Cancer Res 71:3563-72. 2011..Altogether, our experiments validate an important role of miR-10b in gliomagenesis, reveal a novel mechanism of miR-10b-mediated regulation, and suggest the possibility of its future use as a therapeutic target in gliomas... - A secreted luciferase for ex vivo monitoring of in vivo processesThomas Wurdinger
Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, 149 13th St, Charlestown, Massachusetts 02129, USA
Nat Methods 5:171-3. 2008.... - Combined targeting of HER2 and VEGFR2 for effective treatment of HER2-amplified breast cancer brain metastasesDavid P Kodack
Edwin L Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Proc Natl Acad Sci U S A 109:E3119-27. 2012..These findings support the clinical development of this three-drug regimen for the treatment of HER2-amplified breast cancer brain metastases... - miR-296 regulates growth factor receptor overexpression in angiogenic endothelial cellsThomas Wurdinger
Department of Neurology, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Boston, MA 02115, USA
Cancer Cell 14:382-93. 2008..Furthermore, inhibition of miR-296 with antagomirs reduces angiogenesis in tumor xenografts in vivo... 
A highly sensitive assay for monitoring the secretory pathway and ER stressChristian E Badr
Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital, Boston, Massachusetts, United States of America Program in Neuroscience, Harvard Medical School, Boston, Massachusetts, United States of America
PLoS ONE 2:e571. 2007..We have developed a highly sensitive assay to monitor processing of proteins through the secretory pathway and endoplasmic reticulum (ER) stress in real-time based on the naturally secreted Gaussia luciferase (Gluc)...- Gaussia luciferase variant for high-throughput functional screening applicationsCasey A Maguire
Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, Boston Massachusetts 02115, USA
Anal Chem 81:7102-6. 2009..We isolated a Gluc mutant that catalyzes enhanced light stability in the presence of a detergent, in combination with high sensitivity, making it an attractive luciferase for high-throughput functional screening applications... - Microvesicle-associated AAV vector as a novel gene delivery systemCasey A Maguire
Department of Neurology, Massachusetts General Hospital, and Neuroscience Program, Harvard Medical School, Boston, Massachusetts, USA
Mol Ther 20:960-71. 2012..Finally, we show that vexosomes bound to magnetic beads can be attracted to a magnetized area in cultured cells. Vexosomes represent a unique entity which offers a promising strategy to improve gene delivery... - Performance of the red-shifted fluorescent proteins in deep-tissue molecular imaging applicationsNikolaos C Deliolanis
Massachusetts General Hospital and Harvard Medical School, Center for Molecular Imaging Research, Laboratory for Bio optics and Molecular Imaging, Charlestown, Massachusetts 02139, USA
J Biomed Opt 13:044008. 2008.... - Glioma angiogenesis: Towards novel RNA therapeuticsThomas Wurdinger
Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, USA
Cell Adh Migr 3:230-5. 2009..In this review we will discuss the emerging technologies in anti-angiogenesis emphasizing on RNA-based therapeutics... - Multimodal in vivo imaging and blood monitoring of intrinsic and extrinsic apoptosisJohanna M Niers
Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Charlestown, Massachusetts, USA
Mol Ther 19:1090-6. 2011..This reporter provides a valuable tool for imaging and real-time monitoring of apoptosis and is compatible with high-throughput functional screening application in cultured cells and animal models... - Sensitive assay for mycoplasma detection in mammalian cell cultureM Hannah Degeling
Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Harvard Medical School, Boston, Massachusetts 02114, USA
Anal Chem 84:4227-32. 2012..The Gaussia luciferase mycosensor assay provides an easy tool to monitor mammalian cell contaminants in a high-throughput fashion... - Secreted Gaussia luciferase as a biomarker for monitoring tumor progression and treatment response of systemic metastasesEuiheon Chung
Edwin L Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
PLoS ONE 4:e8316. 2009..To this end, we engineered tumor cells to express a naturally secreted Gaussia luciferase (Gluc), and investigated its use as a circulating biomarker for monitoring viable metastatic or primary tumor growth and their treatment responses... - Real-time monitoring of nuclear factor kappaB activity in cultured cells and in animal modelsChristian E Badr
Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Charlestown, 02129, USA
Mol Imaging 8:278-90. 2009..This NF-kappaB reporter system provides a powerful tool for monitoring NF-kappaB activity in real time in vitro and in vivo... - Downregulated microRNA-200a in meningiomas promotes tumor growth by reducing E-cadherin and activating the Wnt/beta-catenin signaling pathwayOkay Saydam
Department of Neurology, Massachusetts General Hospital, and Neuroscience Program, Harvard Medical School, Boston, Massachusetts 02129, USA
Mol Cell Biol 29:5923-40. 2009.... - Codon-optimized Luciola italica luciferase variants for mammalian gene expression in culture and in vivoCasey A Maguire
Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
Mol Imaging 11:13-21. 2012..Overall, our characterization of these codon-optimized luciferases lays the groundwork for their further use as bioluminescent reporters in mammalian cells... - Enhanced Gaussia luciferase blood assay for monitoring of in vivo biological processesM Sarah S Bovenberg
Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, United States
Anal Chem 84:1189-92. 2012..This assay showed to be over 1 order of magnitude more sensitive in detecting Gluc in the blood as compared to the direct Gluc blood assay enhancing ex vivo monitoring of biological processes... - Lanatoside C sensitizes glioblastoma cells to tumor necrosis factor-related apoptosis-inducing ligand and induces an alternative cell death pathwayChristian E Badr
Neuroscience Center and Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA
Neuro Oncol 13:1213-24. 2011..Activation of such mechanism may be a useful strategy to counter resistance of cancer cells to apoptosis... - Metabolic biotinylation of cell surface receptors for in vivo imagingBakhos A Tannous
Center for Molecular Imaging Research, Massachusetts General Hospital-East, Building 149, 13th Street, Charlestown, Massachusetts 02129, USA
Nat Methods 3:391-6. 2006..This BAP-TM allows noninvasive real-time imaging of any cell type transduced to express this reporter protein in culture or in vivo... - Enhanced in vivo imaging of metabolically biotinylated cell surface reportersJohanna M Niers
Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
Anal Chem 83:994-9. 2011..These findings have practical applications in designing more efficient targeting and imaging strategies... - Functional drug screening assay reveals potential glioma therapeuticsChristian E Badr
Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, USA
Assay Drug Dev Technol 9:281-9. 2011..In conclusion, the Gluc secretion assay is a robust tool for functional drug screening and can be applied to many different fields including cancer... - siRNA knock-down of mutant torsinA restores processing through secretory pathway in DYT1 dystonia cellsJeffrey W Hewett
Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA
Hum Mol Genet 17:1436-45. 2008..The ability of allele-specific siRNA for torsinADeltaE to normalize secretory function in DYT1 patient cells supports its potential role as a therapeutic agent in early onset torsion dystonia... 
Bioluminescence imaging: progress and applicationsChristian E Badr
Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA
Trends Biotechnol 29:624-33. 2011..In this review, we discuss current trends in bioluminescence and its application in different fields with an emphasis on cancer research...- T7 RNA polymerase as a self-replicating label for antigen quantificationBakhos A Tannous
Department of Chemistry and Biochemistry, University of Windsor, Ontario N9B 3P4, Canada
Nucleic Acids Res 30:e140. 2002..Furthermore, the T7RP is biotinylated, complexed with streptavidin and used for antigen quantification in a microtiter well-based assay with high sensitivity and reproducibility...