Junichi Takagi

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi request reprint C-terminal opening mimics 'inside-out' activation of integrin alpha5beta1
    J Takagi
    The Center for Blood Research, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Struct Biol 8:412-6. 2001
  2. ncbi request reprint [First atomic view of alpha V beta 3 integrin extracellular domain]
    Junichi Takagi
    Tanpakushitsu Kakusan Koso 47:153-9. 2002
  3. ncbi request reprint The role of the specificity-determining loop of the integrin beta subunit I-like domain in autonomous expression, association with the alpha subunit, and ligand binding
    Junichi Takagi
    The Center for Blood Research and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Biochemistry 41:4339-47. 2002
  4. ncbi request reprint The relative influence of metal ion binding sites in the I-like domain and the interface with the hybrid domain on rolling and firm adhesion by integrin alpha4beta7
    JianFeng Chen
    The CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    J Biol Chem 279:55556-61. 2004
  5. ncbi request reprint Locking the beta3 integrin I-like domain into high and low affinity conformations with disulfides
    Bing Hao Luo
    CBR Institute fo Biomedical Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:10215-21. 2004
  6. ncbi request reprint Allosteric beta1 integrin antibodies that stabilize the low affinity state by preventing the swing-out of the hybrid domain
    Bing Hao Luo
    The CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:27466-71. 2004
  7. pmc Disrupting integrin transmembrane domain heterodimerization increases ligand binding affinity, not valency or clustering
    Bing Hao Luo
    The CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:3679-84. 2005
  8. ncbi request reprint Structures of the alpha L I domain and its complex with ICAM-1 reveal a shape-shifting pathway for integrin regulation
    Motomu Shimaoka
    The Center for Blood Research, Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Cell 112:99-111. 2003
  9. pmc A specific interface between integrin transmembrane helices and affinity for ligand
    Bing Hao Luo
    Center for Blood Research, Institute for Biomedical Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Biol 2:e153. 2004
  10. ncbi request reprint Structural basis for allostery in integrins and binding to fibrinogen-mimetic therapeutics
    Tsan Xiao
    The CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 432:59-67. 2004

Collaborators

Detail Information

Publications37

  1. ncbi request reprint C-terminal opening mimics 'inside-out' activation of integrin alpha5beta1
    J Takagi
    The Center for Blood Research, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Struct Biol 8:412-6. 2001
    ..These findings suggest that the overall mechanism for integrin inside-out activation involves the spatial separation of the cytoplasmic and/or transmembrane domains...
  2. ncbi request reprint [First atomic view of alpha V beta 3 integrin extracellular domain]
    Junichi Takagi
    Tanpakushitsu Kakusan Koso 47:153-9. 2002
  3. ncbi request reprint The role of the specificity-determining loop of the integrin beta subunit I-like domain in autonomous expression, association with the alpha subunit, and ligand binding
    Junichi Takagi
    The Center for Blood Research and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Biochemistry 41:4339-47. 2002
    ....
  4. ncbi request reprint The relative influence of metal ion binding sites in the I-like domain and the interface with the hybrid domain on rolling and firm adhesion by integrin alpha4beta7
    JianFeng Chen
    The CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    J Biol Chem 279:55556-61. 2004
    ..Overall, the results demonstrate the importance of headpiece allostery in the conversion of rolling to firm adhesion...
  5. ncbi request reprint Locking the beta3 integrin I-like domain into high and low affinity conformations with disulfides
    Bing Hao Luo
    CBR Institute fo Biomedical Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:10215-21. 2004
    ..e. that axial movement in the C-terminal direction of the alpha7-helix is linked to rearrangement of the I-like domain metal ion-dependent adhesion site into a high affinity conformation...
  6. ncbi request reprint Allosteric beta1 integrin antibodies that stabilize the low affinity state by preventing the swing-out of the hybrid domain
    Bing Hao Luo
    The CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:27466-71. 2004
    ....
  7. pmc Disrupting integrin transmembrane domain heterodimerization increases ligand binding affinity, not valency or clustering
    Bing Hao Luo
    The CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:3679-84. 2005
    ..Our studies provide evidence that disrupting the integrin heterodimeric TM helix-helix interface activates ligand binding mainly by increasing the monomeric affinity for ligand, but not the receptor valency, i.e., clustering...
  8. ncbi request reprint Structures of the alpha L I domain and its complex with ICAM-1 reveal a shape-shifting pathway for integrin regulation
    Motomu Shimaoka
    The Center for Blood Research, Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Cell 112:99-111. 2003
    ..Pulling down on the C-terminal alpha 7 helix with introduced disulfide bonds ratchets the beta 6-alpha 7 loop into three different positions in the closed, intermediate, and open conformations, with a progressive increase in affinity...
  9. pmc A specific interface between integrin transmembrane helices and affinity for ligand
    Bing Hao Luo
    Center for Blood Research, Institute for Biomedical Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Biol 2:e153. 2004
    ....
  10. ncbi request reprint Structural basis for allostery in integrins and binding to fibrinogen-mimetic therapeutics
    Tsan Xiao
    The CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 432:59-67. 2004
    ..Allostery in the head thus disrupts interaction between the legs in a previously described low-affinity bent integrin conformation, and leg extension positions the high-affinity head far above the cell surface...
  11. pmc Structure of integrin alpha5beta1 in complex with fibronectin
    Junichi Takagi
    The Center for Blood Research, Department of Pediatrics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    EMBO J 22:4607-15. 2003
    ..This finding is corroborated by kinetic analysis of real-time binding data, which shows that the synergy site greatly enhances k(on) but has little effect on the stability or k(off) of the complex...
  12. ncbi request reprint Complex between nidogen and laminin fragments reveals a paradigmatic beta-propeller interface
    Junichi Takagi
    The Center for Blood Research, Boston, Massachusetts 02115, USA
    Nature 424:969-74. 2003
    ....
  13. pmc Stabilizing the open conformation of the integrin headpiece with a glycan wedge increases affinity for ligand
    Bing Hao Luo
    Center for Blood Research and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:2403-8. 2003
    ....
  14. pmc Intersubunit signal transmission in integrins by a receptor-like interaction with a pull spring
    Wei Yang
    The CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:2906-11. 2004
    ....
  15. ncbi request reprint Integrin activation and structural rearrangement
    Junichi Takagi
    The Center for Blood Research, Department of Pathology, Harvard Medical School, Boston 02115, Massachusetts, USA
    Immunol Rev 186:141-63. 2002
    ....
  16. ncbi request reprint Conformational regulation of integrin structure and function
    Motomu Shimaoka
    The Center for Blood Research, Department of Pathology and Anesthesia, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Annu Rev Biophys Biomol Struct 31:485-516. 2002
    ....
  17. ncbi request reprint Global conformational rearrangements in integrin extracellular domains in outside-in and inside-out signaling
    Junichi Takagi
    The Center for Blood Research, Departments of Pathology and Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Cell 110:599-11. 2002
    ..Breakage of a C-terminal clasp between the alpha and beta subunits enhances Mn(2+)-induced unbending and ligand binding...
  18. ncbi request reprint High affinity ligand binding by integrins does not involve head separation
    Bing Hao Luo
    Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 278:17185-9. 2003
    ..The mechanisms of conformational regulation of integrin function should therefore be considered in the context of the associated alphabeta headpiece...
  19. pmc Stabilizing the integrin alpha M inserted domain in alternative conformations with a range of engineered disulfide bonds
    Motomu Shimaoka
    Center for Blood Research, Departments of Pathology, Pediatrics, and Anesthesia, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 99:16737-41. 2002
    ..By contrast, a disulfide stabilizing the closed conformation resulted in inactive alphaMbeta2 that was resistant to activation but became activatable after dithiothreitol treatment...
  20. ncbi request reprint Cysteine-rich module structure reveals a fulcrum for integrin rearrangement upon activation
    Natalia Beglova
    Department of Pathology, Harvard Medical School, 75 Francis St, Boston, Masschusetts 02115, USA
    Nat Struct Biol 9:282-7. 2002
    ....
  21. ncbi request reprint Archaeal surface layer proteins contain beta propeller, PKD, and beta helix domains and are related to metazoan cell surface proteins
    Hua Jing
    Center for Blood Research and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Structure 10:1453-64. 2002
    ..The third domain is predicted to adopt a beta helix fold. These domains have homologs in metazoan cell surface proteins, suggesting remarkable relationships between domains in archaeal SLPs and metazoan cell surface proteins...
  22. pmc The thioesterase domain from a nonribosomal peptide synthetase as a cyclization catalyst for integrin binding peptides
    Rahul M Kohli
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 99:1247-52. 2002
    ..This study provides evidence for TycC TE as a versatile macrocyclization catalyst and raises the prospect of using TE catalysis for the generation of diverse macrocyclic peptide libraries that can be probed for novel biological function...
  23. pmc Integrin beta3 regions controlling binding of murine mAb 7E3: implications for the mechanism of integrin alphaIIbbeta3 activation
    Andrea Artoni
    Laboratory of Blood and Vascular Biology, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 101:13114-20. 2004
    ..Thus, our data are consistent with alphaIIbbeta3 existing in variably bent conformations in equilibrium with each other on unactivated platelets, and activation resulting in alphaIIbbeta3 adopting a more extended conformation...
  24. pmc The RGD motif in fibronectin is essential for development but dispensable for fibril assembly
    Seiichiro Takahashi
    Max Planck Institute of Biochemistry, Department of Molecular Medicine, 82152 Martinsried, Germany
    J Cell Biol 178:167-78. 2007
    ..Our findings demonstrate that FN contains a novel motif for integrin binding and fibril formation whose activity is controlled by amino acid modification...
  25. pmc Structure of a receptor-binding fragment of reelin and mutational analysis reveal a recognition mechanism similar to endocytic receptors
    Norihisa Yasui
    Laboratory of Protein Synthesis and Expression, Institute for Protein Research, Osaka University, 3 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 104:9988-93. 2007
    ....
  26. ncbi request reprint alphaVbeta6 is a novel receptor for human fibrillin-1. Comparative studies of molecular determinants underlying integrin-rgd affinity and specificity
    Jelena Jovanovic
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    J Biol Chem 282:6743-51. 2007
    ..Non-focal contact distribution of alpha5beta1 suggests that its engagement by fibrillin-1 may elicit a lesser degree and/or different type of intracellular signaling compared with that seen with a high affinity ligand...
  27. pmc Structure of a signaling-competent reelin fragment revealed by X-ray crystallography and electron tomography
    Terukazu Nogi
    Laboratory of Protein Synthesis and Expression, Institute for Protein Research, Osaka University, Suita, Osaka, Japan
    EMBO J 25:3675-83. 2006
    ....
  28. ncbi request reprint Direct interaction of the kringle domain of urokinase-type plasminogen activator (uPA) and integrin alpha v beta 3 induces signal transduction and enhances plasminogen activation
    Takehiko Tarui
    Department of Dermatology, UC Davis Medical Center, Research III, Sacramento, CA 95817, USA
    Thromb Haemost 95:524-34. 2006
    ..The uPA kringle-integrin interaction may represent a novel therapeutic target for cancer, inflammation, and vascular remodeling...
  29. ncbi request reprint [Complex between nidogen and laminin fragments reveals a paradigmatic beta-propeller interface]
    Junichi Takagi
    Tanpakushitsu Kakusan Koso 48:1920-7. 2003
  30. pmc Potentiation of the ligand-binding activity of integrin alpha3beta1 via association with tetraspanin CD151
    Ryoko Nishiuchi
    Institute for Protein Research and Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 102:1939-44. 2005
    ....
  31. ncbi request reprint Ligand-binding specificities of laminin-binding integrins: a comprehensive survey of laminin-integrin interactions using recombinant alpha3beta1, alpha6beta1, alpha7beta1 and alpha6beta4 integrins
    Ryoko Nishiuchi
    Laboratory of Extracellular Matrix Biochemistry, Institute for Protein Research, Osaka University, Suita, Osaka, 565 0871, Japan
    Matrix Biol 25:189-97. 2006
    ....
  32. ncbi request reprint [Dynamic regulation of receptor transmembrane domain assembly during signal transmission across the plasma membrane]
    Junichi Takagi
    Tanpakushitsu Kakusan Koso 50:1226-32. 2005
  33. ncbi request reprint [New era of structural analysis of proteins by electron microscopy-application to a dynamic structural analysis of integrin cell adhesion receptor-]
    Kenji Iwasaki
    Research Center for Ultra High Voltage Electron Microcroscopy, Osaka University, Ibraraki, Osaka, Japan
    Seikagaku 77:400-10. 2005
  34. ncbi request reprint Fibronectin peptides derived from two distinct regions stimulate adipocyte differentiation by preventing fibronectin matrix assembly
    Sadahiro Kamiya
    Department of Patho Physiology and Analytical Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, 12 Ichigaya Funagawara machi, Shinjuku ku, Tokyo 162 0826, Japan
    Biochemistry 41:3270-7. 2002
    ....
  35. ncbi request reprint Structural basis for ligand recognition by integrins
    Junichi Takagi
    Laboratory of Protein Synthesis and Expression, Institute for Protein Research, Osaka University, 3 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Curr Opin Cell Biol 19:557-64. 2007
    ....
  36. doi request reprint Milk fat globule EGF factor 8 in the serum of human patients of systemic lupus erythematosus
    Hiroshi Yamaguchi
    Department of Medical Chemistry, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo, Kyoto 606 8501, Japan
    J Leukoc Biol 83:1300-7. 2008
    ..These results suggested that the aberrant expression of MFG-E8 is involved in the pathoetiology of some cases of hSLE...
  37. pmc Key interactions in integrin ectodomain responsible for global conformational change detected by elastic network normal-mode analysis
    Atsushi Matsumoto
    Quantum Bioinformatics Team, Center for Computational Science and Engineering, Japan Atomic Energy Agency, 8 1 Umemidai, Kizugawa, Kyoto 619 0215, Japan
    Biophys J 95:2895-908. 2008
    ..This conservation pattern suggests that the molecular mechanism of the conformational change relying on the interactions found in integrin alpha(V)beta(3) is unique to the limited types of integrins...