M Sykes

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi request reprint Chimerism and central tolerance
    M Sykes
    Bone Marrow Transplantation Section, Massachusetts General Hospital East, Boston 02129, USA
    Curr Opin Immunol 8:694-703. 1996
  2. ncbi request reprint Mixed lymphohaemopoietic chimerism and graft-versus-lymphoma effects after non-myeloablative therapy and HLA-mismatched bone-marrow transplantation
    M Sykes
    Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital and Harvard Medical School, Boston 02129, USA
    Lancet 353:1755-9. 1999
  3. ncbi request reprint Dose and timing of interleukin (IL)-12 and timing and type of total-body irradiation: effects on graft-vs.-host disease inhibition and toxicity of exogenous IL-12 in murine bone marrow transplant recipients
    M Sykes
    Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital Harvard Medical School, Boston 02129, USA
    Biol Blood Marrow Transplant 5:277-84. 1999
  4. ncbi request reprint CD4 T cell-mediated alloresistance to fully MHC-mismatched allogeneic bone marrow engraftment is dependent on CD40-CD40 ligand interactions, and lasting T cell tolerance is induced by bone marrow transplantation with initial blockade of this pathway
    H Ito
    Bone Marrow Transplantation Section, Transplantation Biology Research Center, Massachusetts General Hospital, Boston, MA 02129, USA
    J Immunol 166:2970-81. 2001
  5. ncbi request reprint Tolerization of Gal alpha 1,3Gal-reactive B cells in pre-sensitized alpha 1,3-galactosyltransferase-deficient mice by nonmyeloablative induction of mixed chimerism
    H Ohdan
    Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA
    Xenotransplantation 8:227-38. 2001
  6. ncbi request reprint Enhanced CD4 reconstitution by grafting neonatal porcine tissue in alternative locations is associated with donor-specific tolerance and suppression of preexisting xenoreactive T cells
    J I Rodriguez-Barbosa
    BMT Section, Transplantation Biology Research Center, Massachusetts General Hospital / Harvard Medical School, Boston 02129, USA
    Transplantation 72:1223-31. 2001
  7. ncbi request reprint Both gamma delta T cells and NK cells inhibit the engraftment of xenogeneic rat bone marrow cells and the induction of xenograft tolerance in mice
    B Nikolic
    Bone Marrow Transplantation Section, Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA
    J Immunol 166:1398-404. 2001
  8. ncbi request reprint Induction of stable long-term mixed hematopoietic chimerism following nonmyeloablative conditioning with T cell-depleting antibodies, cyclophosphamide, and thymic irradiation leads to donor-specific in vitro and in vivo tolerance
    M Y Mapara
    Transplantation Biology Research Center, Bone Marrow Transplantation Section, Massachusetts General Hospital, Harvard Medical School, Boston 02129, USA
    Biol Blood Marrow Transplant 7:646-55. 2001
  9. ncbi request reprint Maturation and function of mouse T-cells with a transgenic TCR positively selected by highly disparate xenogeneic porcine MHC
    Y Zhao
    Bone Marrow Transplantation Section, Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital/Harvard Medical School, Boston 02129, USA
    Cell Mol Biol (Noisy-le-grand) 47:217-28. 2001
  10. ncbi request reprint T cell and B cell tolerance to GALalpha1,3GAL-expressing heart xenografts is achieved in alpha1,3-galactosyltransferase-deficient mice by nonmyeloablative induction of mixed chimerism
    H Ohdan
    Bone Marrow Transplantation Section, Transplantation Biology Research Center, Massachusetts General Hospital, MGH East, Building 149-5102, 13th Street, Boston, MA 02129, USA
    Transplantation 71:1532-42. 2001

Collaborators

Detail Information

Publications40

  1. ncbi request reprint Chimerism and central tolerance
    M Sykes
    Bone Marrow Transplantation Section, Massachusetts General Hospital East, Boston 02129, USA
    Curr Opin Immunol 8:694-703. 1996
    ..Advances have been made in the ability to achieve this permissive state without toxic, myeloablative conditioning, thus bringing this approach closer to clinical application...
  2. ncbi request reprint Mixed lymphohaemopoietic chimerism and graft-versus-lymphoma effects after non-myeloablative therapy and HLA-mismatched bone-marrow transplantation
    M Sykes
    Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital and Harvard Medical School, Boston 02129, USA
    Lancet 353:1755-9. 1999
    ..We tested a new approach to find out whether lymphohaemopoietic graft-versus-host reactions could occur without excessive GVHD in mixed haemopoietic chimeras produced across HLA barriers with non-myeloablative conditioning...
  3. ncbi request reprint Dose and timing of interleukin (IL)-12 and timing and type of total-body irradiation: effects on graft-vs.-host disease inhibition and toxicity of exogenous IL-12 in murine bone marrow transplant recipients
    M Sykes
    Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital Harvard Medical School, Boston 02129, USA
    Biol Blood Marrow Transplant 5:277-84. 1999
    ..Careful studies are warranted to test the effects of IL-12 in the context of BMT with various conditioning regimens in large animal preclinical models before this novel approach to GVHD protection can be applied clinically...
  4. ncbi request reprint CD4 T cell-mediated alloresistance to fully MHC-mismatched allogeneic bone marrow engraftment is dependent on CD40-CD40 ligand interactions, and lasting T cell tolerance is induced by bone marrow transplantation with initial blockade of this pathway
    H Ito
    Bone Marrow Transplantation Section, Transplantation Biology Research Center, Massachusetts General Hospital, Boston, MA 02129, USA
    J Immunol 166:2970-81. 2001
    ..Exposure to donor bone marrow allows rapid tolerization of alloreactive CD4 cells when the CD40 pathway is blocked, leading to permanent marrow engraftment and intrathymic tolerization of T cells that develop subsequently...
  5. ncbi request reprint Tolerization of Gal alpha 1,3Gal-reactive B cells in pre-sensitized alpha 1,3-galactosyltransferase-deficient mice by nonmyeloablative induction of mixed chimerism
    H Ohdan
    Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA
    Xenotransplantation 8:227-38. 2001
    ..The absence of B cells with receptors recognizing Gal in mixed chimeras suggests a role for clonal deletion/receptor editing in the maintenance of B cell tolerance...
  6. ncbi request reprint Enhanced CD4 reconstitution by grafting neonatal porcine tissue in alternative locations is associated with donor-specific tolerance and suppression of preexisting xenoreactive T cells
    J I Rodriguez-Barbosa
    BMT Section, Transplantation Biology Research Center, Massachusetts General Hospital / Harvard Medical School, Boston 02129, USA
    Transplantation 72:1223-31. 2001
    ..These results also suggest that there is a suppressive component to the porcine xenograft tolerance induced with this approach...
  7. ncbi request reprint Both gamma delta T cells and NK cells inhibit the engraftment of xenogeneic rat bone marrow cells and the induction of xenograft tolerance in mice
    B Nikolic
    Bone Marrow Transplantation Section, Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA
    J Immunol 166:1398-404. 2001
    ..2 and anti-NK1.1 mAbs in TCRdelta(-/-) mice. Therefore, in addition to CD4 and CD8 T cells, gammadelta T cells and NK cells play a role in resisting engraftment of rat marrow and the induction of xenograft tolerance in mice...
  8. ncbi request reprint Induction of stable long-term mixed hematopoietic chimerism following nonmyeloablative conditioning with T cell-depleting antibodies, cyclophosphamide, and thymic irradiation leads to donor-specific in vitro and in vivo tolerance
    M Y Mapara
    Transplantation Biology Research Center, Bone Marrow Transplantation Section, Massachusetts General Hospital, Harvard Medical School, Boston 02129, USA
    Biol Blood Marrow Transplant 7:646-55. 2001
    ..CONCLUSION: Engraftment, long-term chimerism, and induction of donor-specific tolerance can be achieved using a nonmyeloablative CTX-based conditioning regimen in fully MHC-mismatched BMT recipients without the induction of GVHD...
  9. ncbi request reprint Maturation and function of mouse T-cells with a transgenic TCR positively selected by highly disparate xenogeneic porcine MHC
    Y Zhao
    Bone Marrow Transplantation Section, Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital/Harvard Medical School, Boston 02129, USA
    Cell Mol Biol (Noisy-le-grand) 47:217-28. 2001
    ....
  10. ncbi request reprint T cell and B cell tolerance to GALalpha1,3GAL-expressing heart xenografts is achieved in alpha1,3-galactosyltransferase-deficient mice by nonmyeloablative induction of mixed chimerism
    H Ohdan
    Bone Marrow Transplantation Section, Transplantation Biology Research Center, Massachusetts General Hospital, MGH East, Building 149-5102, 13th Street, Boston, MA 02129, USA
    Transplantation 71:1532-42. 2001
    ....
  11. ncbi request reprint Highly disparate xenogeneic skin graft tolerance induction by fetal pig thymus in thymectomized mice: Conditioning requirements and the role of coimplantation of fetal pig liver
    Y Zhao
    Bone Marrow Transplantation Section, Transplantation Biology Research Center, Surgical Services, Massachusetts General Hospital, Boston, MA 02129, USA
    Transplantation 72:1608-15. 2001
    ..Additional treatment of ATX recipient mice with anti-Thy1.2 and NK1.1 mAbs and 3 Gy TBI is not essential for donor pig skin graft tolerance induction...
  12. ncbi request reprint Porcine stem cell engraftment and seeding of murine thymus with class II+ cells in mice expressing porcine cytokines: toward tolerance induction across discordant xenogeneic barriers
    A M Chen
    Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital Harvard Medical School, Boston 02129, USA
    Transplantation 69:2484-90. 2000
    ..To evaluate the ability of mixed chimerism to induce swine-specific tolerance in widely disparate xenogeneic recipients, this study aimed to achieve long-lasting chimerism in a pig to mouse combination...
  13. ncbi request reprint Peripheral deletion after bone marrow transplantation with costimulatory blockade has features of both activation-induced cell death and passive cell death
    T Wekerle
    BMT Section, Transplantation Biology Research Center, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA
    J Immunol 166:2311-6. 2001
    ..Furthermore, these in vivo data demonstrate for the first time the significance of in vitro results indicating that proteins of the Bcl family can prevent Fas-mediated apoptosis under certain circumstances...
  14. ncbi request reprint Induction of tolerance by mixed chimerism with nonmyeloblative host conditioning: the importance of overcoming intrathymic alloresistance
    B Nikolic
    Bone Marrow Transplantation Section, Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, 02129, USA
    Biol Blood Marrow Transplant 7:144-53. 2001
    ..This downmodulation may play a role in the loss of alloreactivity. Our results suggest that a second MoAb injection inactivates mature, functional donor-alloreactive CD4+ and CD8+ host thymocytes...
  15. ncbi request reprint Mac-1-negative B-1b phenotype of natural antibody-producing cells, including those responding to Gal alpha 1,3Gal epitopes in alpha 1,3-galactosyltransferase-deficient mice
    H Ohdan
    Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital Harvard Medical School, Boston, MA 02129, USA
    J Immunol 165:5518-29. 2000
    ..They are consistent with a model whereby B-1b cells lose Mac-1 expression upon Ag exposure and that these, rather than plasma cells, become the major IgM Ab-producing cell population...
  16. ncbi request reprint Differing mechanisms of early and late B cell hyporesponsiveness induced by mixed chimerism
    T Kawahara
    Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Am J Transplant 5:2821-9. 2005
    ..These results have implications for the potential use of mixed hematopioetic chimerism as an approach to performing organ transplantation in recipients with pre-existing anti-donor IgM antibodies...
  17. ncbi request reprint Cross-species interaction of porcine and human integrins with their respective ligands: implications for xenogeneic tolerance induction
    A R Simon
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston 02129, USA
    Transplantation 66:385-94. 1998
    ....
  18. pmc B-cell immunity in the context of T-cell tolerance after combined kidney and bone marrow transplantation in humans
    F Porcheray
    Transplantation Biology Research Center, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
    Am J Transplant 9:2126-35. 2009
    ..Overall, our findings showed the development of B-cell allo- and autoimmunity in patients with T-cell tolerance to the donor graft...
  19. ncbi request reprint Myeloma responses and tolerance following combined kidney and nonmyeloablative marrow transplantation: in vivo and in vitro analyses
    Y Fudaba
    Transplantation Biology Research Center, Department of Surgery, Massachusetts General Hospital Harvard Medical School, MGH East, Building 149 5102 13th Street, Boston, Massachusetts, USA
    Am J Transplant 6:2121-33. 2006
    ..Combined kidney/BMT with nonmyeloablative conditioning can achieve renal allograft tolerance and excellent myeloma responses, even in the presence of donor marrow rejection and antidonor alloresponses in vitro...
  20. ncbi request reprint Mechanisms involved in the establishment of tolerance through costimulatory blockade and BMT: lack of requirement for CD40L-mediated signaling for tolerance or deletion of donor-reactive CD4+ cells
    J Kurtz
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston 02129, USA
    Am J Transplant 1:339-49. 2001
    ....
  21. ncbi request reprint Development and analysis of transgenic mice expressing porcine hematopoietic cytokines: a model for achieving durable porcine hematopoietic chimerism across an extensive xenogeneic barrier
    Y G Yang
    Bone Marrow Transplantation Section, Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston 02129, USA
    Xenotransplantation 7:58-64. 2000
    ..These Tg mice provide a useful model system for studying porcine hematopoietic stem cells, and for evaluating the feasibility of donor-specific tolerance induction by mixed chimerism across highly disparate xenogeneic barriers...
  22. pmc Induction of human T-cell tolerance to pig xenoantigens via thymus transplantation in mice with an established human immune system
    K Habiro
    Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
    Am J Transplant 9:1324-9. 2009
    ..These data show that porcine thymic xenotransplantation can induce donor-specific tolerance in immunocompetent hu-mice, supporting this approach for tolerance induction in clinical xenotransplantation...
  23. ncbi request reprint In vivo T-cell depletion enhances production of anti-GALalpha1,3GAL natural antibodies in alpha1,3-galactosyltransferase-deficient mice
    H Ohdan
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston 02129, USA
    Transplantation 69:910-3. 2000
    ..It has been reported that T-cell depletion by in vivo treatment with monoclonal antibodies results in polyclonal B-cell activation. However, its effects on B cells responding to Galalpha1,3Gal (Gal) epitopes remain unknown...
  24. ncbi request reprint Tolerance, mixed chimerism, and chronic transplant arteriopathy
    P S Russell
    Department of Surgery, Transplantation Biology Research Center, Harvard Medical School, Massachusetts General Hospital, Boston, MA 02114, USA
    J Immunol 167:5731-40. 2001
    ..Perhaps innate responsiveness, that could include NK cell activity, can create such arteriopathic lesions. More evidence is being sought regarding this process...
  25. pmc Mechanisms of donor-specific tolerance in recipients of haploidentical combined bone marrow/kidney transplantation
    G Andreola
    Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
    Am J Transplant 11:1236-47. 2011
    ..While regulatory cells may play an early role, long-term tolerance appears to be maintained by a deletion or anergy mechanism...
  26. ncbi request reprint Global unresponsiveness as a mechanism of natural killer cell tolerance in mixed xenogeneic chimeras
    T Kawahara
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA, USA
    Am J Transplant 7:2090-7. 2007
    ..Our data suggest that NK-cell anergy is induced by interactions with xenogeneic hematopoietic cells that express activating but not inhibitory ligands for recipient NK cells...
  27. pmc Results of gal-knockout porcine thymokidney xenografts
    A D Griesemer
    Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
    Am J Transplant 9:2669-78. 2009
    ..These results show that xenogeneic thymus transplantation can support early primate thymopoiesis, which in turn may induce T-cell tolerance to solid organ xenografts...
  28. doi request reprint Recipient dendritic cells, but not B cells, are required antigen-presenting cells for peripheral alloreactive CD8+ T-cell tolerance
    J L Mollov
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA, USA
    Am J Transplant 10:518-26. 2010
    ..Moreover, recipient IDO and IL-10 were not required. Thus, antigen presentation by recipient DCs and not by B cells is critical for peripheral alloreactive CD8 T cell tolerance...
  29. ncbi request reprint Specific unresponsiveness to a retrovirally-transferred class I antigen is controlled through the helper pathway
    C C Fraser
    Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston 02129
    J Immunol 154:1587-95. 1995
    ..Tolerance to Kb induced by this approach may therefore be restricted to T helper cell lineages...
  30. ncbi request reprint Immune tolerance: mechanisms and application in clinical transplantation
    M Sykes
    Transplantation Biology Research Center, Bone Marrow Transplantation Section, Massachusetts General Hospital Harvard Medical School, Boston, MA 02129, USA
    J Intern Med 262:288-310. 2007
    ..These advances are reviewed here and the appropriate timing for clinical trials is discussed...
  31. pmc Mixed chimerism
    M Sykes
    Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02129, USA
    Philos Trans R Soc Lond B Biol Sci 356:707-26. 2001
    ....
  32. ncbi request reprint Pig hematopoietic cell chimerism in baboons conditioned with a nonmyeloablative regimen and CD154 blockade
    L Buhler
    Transplantation Biology Research Center, Massachusetts General Hospital, MGH East, Building 149 9019, 13th Street, Boston, MA 02129, USA
    Transplantation 73:12-22. 2002
    ....
  33. pmc Th1 and Th2 mediate acute graft-versus-host disease, each with distinct end-organ targets
    B Nikolic
    Bone Marrow Transplantation Section, Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts 02129, USA
    J Clin Invest 105:1289-98. 2000
    ..Our studies demonstrate an additive role for Th1 and Th2 cells in producing acute GVHD, and suggest a cytokine-directed approach to treating end-organ manifestations of GVHD...
  34. pmc Mixed chimerism induced without lethal conditioning prevents T cell- and anti-Gal alpha 1,3Gal-mediated graft rejection
    H Ohdan
    Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital Harvard Medical School, Boston 02129, USA
    J Clin Invest 104:281-90. 1999
    ....
  35. ncbi request reprint Efficacy of adhesive interactions in pig-to-human xenotransplantation
    A R Simon
    Bone Marrow Transplantation Section, Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital Harvard Medical School, MGH East, Building 149 5102, 13th Street, Boston, MA 02129, USA
    Immunol Today 20:323-30. 1999
    ..Here, André Simon, Anthony Warrens and Megan Sykes review the existing information on pig-to-human adhesive interactions and its implication for different approaches to pig-to-human xenotransplantation...
  36. ncbi request reprint Hematopoietic stem cell quiescence maintained by p21cip1/waf1
    T Cheng
    Experimental Hematology, AIDS Research Center, Massachusetts General Hospital Cancer Center, Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
    Science 287:1804-8. 2000
    ..Under conditions of stress, restricted cell cycling is crucial to prevent premature stem cell depletion and hematopoietic death...
  37. ncbi request reprint American society of transplantation symposium on B cells in transplantation: harnessing humoral immunity from rodent models to clinical practice
    A D Kirk
    The Transplantation Branch, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA
    Am J Transplant 7:1464-70. 2007
    ....
  38. ncbi request reprint Comparison of outcomes after transplantation of peripheral blood stem cells versus bone marrow following an identical nonmyeloablative conditioning regimen
    B R Dey
    Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Bone Marrow Transplant 40:19-27. 2007
    ..CD4 and CD8 T-cell recovery was faster in PBSC recipients. In PBSC recipients, a higher number of CD34+ cells was associated with increased rates of severe, grade III-IV acute GVHD...
  39. pmc Depletion of CD8 memory T cells for induction of tolerance of a previously transplanted kidney allograft
    I Koyama
    Department of Surgery, Transplantation Unit, Harvard Medical School at Massachusetts General Hospital, Boston, MA, USA
    Am J Transplant 7:1055-61. 2007
    ..The current studies provide 'proof of principle' that the mixed chimerism approach can induce renal allograft tolerance, even late after organ transplantation if memory T-cell function is adequately controlled...
  40. ncbi request reprint Long-term islet allograft function in the absence of chronic immunosuppression: a case report of a nonhuman primate previously made tolerant to a renal allograft from the same donor
    T Kawai
    Department of Surgery, Harvard Medical School and the Transplantation Unit, Massachusetts General Hospital, White 510, 55 Fruit Street, Boston, MA 02114, USA
    Transplantation 72:351-4. 2001
    ..Because no histological evidence of rejection was identified, recurrent diabetes is presumed to be inadequate islet mass...