T P Stossel

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi request reprint Regulation of financial conflicts of interest in medical practice and medical research: a damaging solution in search of a problem
    Thomas P Stossel
    Department of Medicine, Harvard Medical School, Brigham and Women s Hospital, Boston, MA 02115, USA
    Perspect Biol Med 50:54-71. 2007
  2. ncbi request reprint Filamins as integrators of cell mechanics and signalling
    T P Stossel
    Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 2:138-45. 2001
  3. pmc Mechanical strain in actin networks regulates FilGAP and integrin binding to filamin A
    A J Ehrlicher
    Translational Medicine Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 478:260-3. 2011
  4. ncbi request reprint Mechanisms of cold-induced platelet actin assembly
    K M Hoffmeister
    Division of Hematology, Brigham and Women's Hospital, Harvard Medical School, 221 Longwood Ave, Boston, MA 02115, USA
    J Biol Chem 276:24751-9. 2001
  5. pmc Filamin A, the Arp2/3 complex, and the morphology and function of cortical actin filaments in human melanoma cells
    L A Flanagan
    Hematology Division, Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 155:511-7. 2001
  6. ncbi request reprint Deconstructing gelsolin: identifying sites that mimic or alter binding to actin and phosphoinositides
    P A Janmey
    Experimental Medicine Division, Brigham and Women s Hospital, Boston, MA 02115, USA
    Chem Biol 5:R81-5. 1998
  7. pmc Thiol oxidation of actin produces dimers that enhance the elasticity of the F-actin network
    J X Tang
    Hematology Division, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Biophys J 76:2208-15. 1999
  8. ncbi request reprint Cell permeant polyphosphoinositide-binding peptides that block cell motility and actin assembly
    C C Cunningham
    Hematology Division, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 276:43390-9. 2001
  9. ncbi request reprint Signaling pathways and cell mechanics involved in wound closure by epithelial cell sheets
    G Fenteany
    Division of Hematology, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Curr Biol 10:831-8. 2000
  10. ncbi request reprint Actin-binding protein-280 binds the stress-activated protein kinase (SAPK) activator SEK-1 and is required for tumor necrosis factor-alpha activation of SAPK in melanoma cells
    A Marti
    Diabetes Unit and Medical Services, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 021291, USA
    J Biol Chem 272:2620-8. 1997

Collaborators

Detail Information

Publications40

  1. ncbi request reprint Regulation of financial conflicts of interest in medical practice and medical research: a damaging solution in search of a problem
    Thomas P Stossel
    Department of Medicine, Harvard Medical School, Brigham and Women s Hospital, Boston, MA 02115, USA
    Perspect Biol Med 50:54-71. 2007
    ..Most ominously, it has imposed onerous and counterproductive regulations on medical education and translational research...
  2. ncbi request reprint Filamins as integrators of cell mechanics and signalling
    T P Stossel
    Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 2:138-45. 2001
    ..They integrate cellular architectural and signalling functions and are essential for fetal development and cell locomotion. Here, we describe the history, structure and function of this group of proteins...
  3. pmc Mechanical strain in actin networks regulates FilGAP and integrin binding to filamin A
    A J Ehrlicher
    Translational Medicine Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 478:260-3. 2011
    ..These results identify a molecular mechanotransduction element within the actin cytoskeleton, revealing that mechanical strain of key proteins regulates the binding of signalling molecules...
  4. ncbi request reprint Mechanisms of cold-induced platelet actin assembly
    K M Hoffmeister
    Division of Hematology, Brigham and Women's Hospital, Harvard Medical School, 221 Longwood Ave, Boston, MA 02115, USA
    J Biol Chem 276:24751-9. 2001
    ....
  5. pmc Filamin A, the Arp2/3 complex, and the morphology and function of cortical actin filaments in human melanoma cells
    L A Flanagan
    Hematology Division, Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 155:511-7. 2001
    ..Contrary to some proposals, Arp2/3 complex-mediated branching of actin alone is insufficient for establishing an orthogonal actin organization or maintaining mechanical stability at the leading edge...
  6. ncbi request reprint Deconstructing gelsolin: identifying sites that mimic or alter binding to actin and phosphoinositides
    P A Janmey
    Experimental Medicine Division, Brigham and Women s Hospital, Boston, MA 02115, USA
    Chem Biol 5:R81-5. 1998
    ..Recent advances in defining the structure of gelsolin identified functionally important sites. These structural insights could lead to the design of small molecule analogs to enhance, inhibit or mimic the functions of gelsolin...
  7. pmc Thiol oxidation of actin produces dimers that enhance the elasticity of the F-actin network
    J X Tang
    Hematology Division, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Biophys J 76:2208-15. 1999
    ..These new findings may explain why previous studies of actin rheology report a wide range of values when purified actin is polymerized without added regulatory proteins...
  8. ncbi request reprint Cell permeant polyphosphoinositide-binding peptides that block cell motility and actin assembly
    C C Cunningham
    Hematology Division, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 276:43390-9. 2001
    ..This class of reagents confirms the critical relation between inositol lipids and cytoskeletal structure and may be useful to probe the location and function of polyphosphoinositides in vivo...
  9. ncbi request reprint Signaling pathways and cell mechanics involved in wound closure by epithelial cell sheets
    G Fenteany
    Division of Hematology, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Curr Biol 10:831-8. 2000
    ..We have used epithelial wound closure as a model system for such movements and examined the mechanisms of closure and the importance of the Rho family of Ras-related small GTPases in this process...
  10. ncbi request reprint Actin-binding protein-280 binds the stress-activated protein kinase (SAPK) activator SEK-1 and is required for tumor necrosis factor-alpha activation of SAPK in melanoma cells
    A Marti
    Diabetes Unit and Medical Services, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 021291, USA
    J Biol Chem 272:2620-8. 1997
    ..ABP-280 participates in TNF-alpha signal transduction to SAPKs, in part through the binding of SEK-1...
  11. ncbi request reprint Hemostatic, inflammatory, and fibroblast responses are blunted in mice lacking gelsolin
    W Witke
    Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 81:41-51. 1995
    ..Neither gelsolin nor other proteins with similar actin filament-severing activity are expressed in early embryonic cells, indicating that this mechanism of actin filament dynamics is not essential for motility during early embryogenesis...
  12. ncbi request reprint Reduction in viscosity of cystic fibrosis sputum in vitro by gelsolin
    C A Vasconcellos
    Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115
    Science 263:969-71. 1994
    ..These results suggest that gelsolin may have therapeutic potential as a mucolytic agent in CF patients...
  13. ncbi request reprint Mechanisms of lysolipid phosphate effects on cellular survival and proliferation
    E J Goetzl
    Department of Medicine, University of California, San Francisco 94143, USA
    Ann N Y Acad Sci 905:177-87. 2000
    ....
  14. ncbi request reprint Comparison of filamin A-induced cross-linking and Arp2/3 complex-mediated branching on the mechanics of actin filaments
    Fumihiko Nakamura
    Hematology Division, Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 277:9148-54. 2002
    ..They suggest complementary roles for actin branching and cross-linking in cellular actin mechanics in vivo...
  15. pmc The structure of the GPIb-filamin A complex
    Fumihiko Nakamura
    Hematology Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    Blood 107:1925-32. 2006
    ..To verify the topology of GPIb-FLNa interaction we also purified the native complex from platelets and showed that GPIb interacts with the C-terminus of FLNa, which is in accordance with our biochemical and structural data...
  16. ncbi request reprint FilGAP, a Rho- and ROCK-regulated GAP for Rac binds filamin A to control actin remodelling
    Yasutaka Ohta
    Hematology Division, Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    Nat Cell Biol 8:803-14. 2006
    ..FilGAP is, therefore, a mediator of the well-established antagonism of Rac by RhoA that suppresses leading edge protrusion and promotes cell retraction to achieve cellular polarity...
  17. ncbi request reprint Modifications of cellular responses to lysophosphatidic acid and platelet-activating factor by plasma gelsolin
    Teresia M Osborn
    Translational Medicine Division Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, 75 Francis St, Boston, MA 02115, USA
    Am J Physiol Cell Physiol 292:C1323-30. 2007
    ..024). Therefore, pGSN interferes with PAF- and LPA-induced cellular activation in vitro, suggesting a mechanism for the protective role of pGSN in vivo...
  18. ncbi request reprint Plasma gelsolin is a marker and therapeutic agent in animal sepsis
    Po Shun Lee
    Pulmonary and Critical Care Division Brigham and Women s Hospital, Boston, MA, USA
    Crit Care Med 35:849-55. 2007
    ..Depletion of plasma gelsolin in systemic inflammation may contribute to adverse outcomes. We examined the role of plasma gelsolin in animal models of sepsis...
  19. ncbi request reprint In vitro function and phagocytosis of galactosylated platelet concentrates after long-term refrigeration
    Aleksandar M Babic
    Department of Pathology, Division of Translational Medicine, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Transfusion 47:442-51. 2007
    ..PLT-associated galactosyltransferase efficiently galactosylates chilled PLTs in the presence of its substrate UDP-galactose is added to PLT-rich plasma...
  20. pmc Disease-associated mutant alpha-actinin-4 reveals a mechanism for regulating its F-actin-binding affinity
    Astrid Weins
    Renal and Translational Medicine Divisions, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:16080-5. 2007
    ....
  21. pmc Structural basis of filamin A functions
    Fumihiko Nakamura
    Translational Medicine Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 179:1011-25. 2007
    ..Dimerization, mediated by FLNa Ig repeat 24, accounts for rigid high-angle FLNa/F-actin branching resistant to bending by thermal forces, and high avidity F-actin binding and cross-linking...
  22. pmc Has the hunt for conflicts of interest gone too far? Yes
    Thomas P Stossel
    Department of Medicine, Harvard Medical School, Translational Medicine and Hematology Divisions, Brigham and Women s Hospital, 1 Blackfan Circle, Karp 6, Boston, MA 02115 USA
    BMJ 336:476. 2008
  23. pmc Arp2/3 complex-deficient mouse fibroblasts are viable and have normal leading-edge actin structure and function
    Alessia Di Nardo
    Division of Hematology, Brigham and Women s Hospital, and Department of Medicine, Harvard Medical School, One Blackfan Circle, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:16263-8. 2005
    ..The Arp2/3 complex can be dispensable for leading-edge actin remodeling...
  24. ncbi request reprint Effect of thrombopoietin alone and a combination of cytochalasin B and ethylene glycol bis(beta-aminoethyl ether) N,N'-tetraacetic acid-AM on the survival and function of autologous baboon platelets stored at 4 degrees C for as long as 5 days
    C Robert Valeri
    Naval Blood Research Laboratory, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Transfusion 44:865-70. 2004
    ....
  25. ncbi request reprint Filamin is essential in actin cytoskeletal assembly mediated by p21-activated kinase 1
    Ratna K Vadlamudi
    Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Nat Cell Biol 4:681-90. 2002
    ....
  26. pmc Importance of free actin filament barbed ends for Arp2/3 complex function in platelets and fibroblasts
    Hervé Falet
    Division of Hematology, Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, 221 Longwood Avenue, LMRC 301, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 99:16782-7. 2002
    ..We conclude that Arp23 complex contribution to actin filament nucleation in platelets and fibroblasts importantly requires free barbed ends generated by severing and uncapping...
  27. ncbi request reprint The clearance mechanism of chilled blood platelets
    Karin M Hoffmeister
    Division of Hematology, Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
    Cell 112:87-97. 2003
    ..Chilled platelets bind vWf and function normally in vitro and ex vivo after transfusion into CR3-deficient mice. Therefore, GPIb modification might permit cold platelet storage...
  28. ncbi request reprint Filling gaps in signaling to actin cytoskeletal remodeling
    Thomas P Stossel
    Hematology Division, Brigham and Women s Hospital, Harvard Medical School, 221 Longwood Avenue, Boston, MA 02115, USA
    Dev Cell 4:444-5. 2003
    ..It describes the identification of a novel membrane component Mig-2 that engages filamin A through a new intermediary, migfilin, to stimulate actin assembly and cell spreading on a substrate of extracellular matrix...
  29. ncbi request reprint Glycosylation restores survival of chilled blood platelets
    Karin M Hoffmeister
    Division of Hematology, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Science 301:1531-4. 2003
    ..Platelet-associated galactosyltransferase produces efficient galactosylation when uridine diphosphate-galactose is added, affording a potentially simple method for storing platelets in the cold...
  30. pmc Ribosomal S6 kinase (RSK) regulates phosphorylation of filamin A on an important regulatory site
    Michele S Woo
    Department of Cell Biology, Brigham and Women s Hospital, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 24:3025-35. 2004
    ..Given that phosphorylation of FLNa on Ser(2152) is required for Pak1-mediated membrane ruffling, our results suggest a novel role for RSK in the regulation of the actin cytoskeleton...
  31. ncbi request reprint The macrophage alphaMbeta2 integrin alphaM lectin domain mediates the phagocytosis of chilled platelets
    Emma C Josefsson
    Division of Hematology, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 280:18025-32. 2005
    ....
  32. ncbi request reprint Ca2+ and calmodulin regulate the binding of filamin A to actin filaments
    Fumihiko Nakamura
    Hematology Division, Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 280:32426-33. 2005
    ..These findings identify the first evidence for direct regulation of FLNa, implicating a mechanism whereby Ca(2+)-calmodulin selectively targets the FLNa.F-actin complex...
  33. ncbi request reprint Cell surface actin remodeling
    Thomas P Stossel
    Hematology Division, Brigham and Women s Hospital, Boston, MA 02115, USA
    J Cell Sci 119:3261-4. 2006
  34. ncbi request reprint Recombinant plasma gelsolin diminishes the acute inflammatory response to hyperoxia in mice
    Melpo Christofidou-Solomidou
    Division of Pulmonary Medicine, Department of Medicine, University of Pennsylvania Medical School, Philadelphia, USA
    J Investig Med 50:54-60. 2002
    ..By scavenging extracellular actin and modulating the effects of lysophosphatidic acid, plasma gelsolin could serve a critical protective role against oxidant injury...
  35. doi request reprint A biopsy of financial conflicts of interest in medicine
    Thomas P Stossel
    Divisions of Translational Medicine and Hematology, Brigham and Women s Hospital, Boston, Mass, USA
    Surgery 143:193-8. 2008
  36. ncbi request reprint Prognostic implications of declining plasma gelsolin levels after allogeneic stem cell transplantation
    Mark J DiNubile
    Division of Infectious Diseases, Department of Medicine, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Camden, NJ, USA
    Blood 100:4367-71. 2002
    ..0007). These findings suggest that gelsolin levels shortly after allogeneic HSCT can predict the later development of fatal IPS. Gelsolin replacement in selected transplant patients may offer a novel strategy to prevent or reverse IPS...
  37. ncbi request reprint Divergent views on managing clinical conflicts of interest
    Thomas P Stossel
    Mayo Clin Proc 82:1013-4; author reply 1014-5. 2007
  38. ncbi request reprint Overregulation of conflicts hinders medical progress
    Thomas P Stossel
    Harvard Medical School, Brigham and Women s Hospital, Boston, MA 02115, USA
    Cleve Clin J Med 74:S14-5; discussion S16-22. 2007
    ..Regulations to prevent conflicts of interest by restricting medical education, medical research, expert advisory functions, or researcher ownership of inventions may have the unintended consequence of slowing medical progress...
  39. pmc Relationship of plasma gelsolin levels to outcomes in critically ill surgical patients
    Po Shun Lee
    Pulmonary and Critical Care Unit, Massachusetts General Hospital, Boston, MA, USA
    Ann Surg 243:399-403. 2006
    ..To examine the relationship between plasma gelsolin levels and mortality following surgery or trauma...
  40. ncbi request reprint Regulating academic-industrial research relationships--solving problems or stifling progress?
    Thomas P Stossel
    Harvard Medical School and the Hematology Division, Brigham and Women s Hospital, Boston, USA
    N Engl J Med 353:1060-5. 2005