Genomes and Genes
Affiliation: Harvard University
- Targeting pan-resistant bacteria with antibodies to a broadly conserved surface polysaccharide expressed during infectionDavid Skurnik
Channing Laboratory, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
J Infect Dis 205:1709-18. 2012....
- Is exposure to mercury a driving force for the carriage of antibiotic resistance genes?David Skurnik
EA3964 Résistance Bactérienne in Vivo, Faculte de Medecine, Université Paris Diderot and Hôpital Bichat Claude Bernard, CNR Résistance Bactérienne dans les Flores Commensales, APHP, 75018 Paris, France
J Med Microbiol 59:804-7. 2010..Exposure to mercury might be a specific driving force for the acquisition and maintenance of mobile antibiotic resistance gene carriage in the absence of antibiotic selective pressure...
- Antibody to a conserved antigenic target is protective against diverse prokaryotic and eukaryotic pathogensColette Cywes-Bentley
Division of Infectious Diseases, Department of Medicine, Brigham and Women s Hospital Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 110:E2209-18. 2013..Vaccination targeting PNAG could contribute to immunity against serious and diverse prokaryotic and eukaryotic pathogens, and the conserved production of PNAG suggests that it is a critical factor in microbial biology...
- Natural antibodies in normal human serum inhibit Staphylococcus aureus capsular polysaccharide vaccine efficacyDavid Skurnik
Channing Laboratory, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
Clin Infect Dis 55:1188-97. 2012..aureus cell surface polysaccharide, poly-N-acetyl glucosamine (PNAG). Here we evaluated whether natural antibody to PNAG in normal human serum (NHS) had a similar deleterious effect...
- Parallel bacterial evolution within multiple patients identifies candidate pathogenicity genesTami D Lieberman
Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA
Nat Genet 43:1275-80. 2011..The identification of parallel molecular evolution as a pathogen spreads among multiple individuals points to the key selection forces it experiences within human hosts...
- Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coliDavid Skurnik
Laboratoire de Bacteriologie, Hopital Bichat Claude Bernard, AP HP and EA3964, Universite Paris 7 Denis Diderot, 46 rue Henri Huchard, 75018 Paris, France
J Antimicrob Chemother 57:1215-9. 2006..To determine the level of antimicrobial resistance and the occurrence of class 1, 2 and 3 integrons in faecal Escherichia coli from several animal populations variously exposed to human contact...
- Organic and conventional fruits and vegetables contain equivalent counts of Gram-negative bacteria expressing resistance to antibacterial agentsRaymond Ruimy
EA 6934 lt lt Résistance Bactérienne in vivo gt gt Université Paris Diderot, and Centre National de Référence sur la Résistance aux Antibiotiques, Hopital Bichat Claude Bernard, APHP, Paris, France
Environ Microbiol 12:608-15. 2010..which grouped into two phylotypes and all carrying the bla(RAHN) gene. Thus, both organic and conventional fruits and vegetables may constitute significant sources of resistant bacteria and of resistance genes...
- Characteristics of human intestinal Escherichia coli with changing environmentsDavid Skurnik
EA3964 lt lt Résistance bactérienne in vivo gt gt Faculté de Médecine, Université Paris 7 and Hôpital Bichat APHP, 75018 Paris, France
Environ Microbiol 10:2132-7. 2008..03). Our results suggest that the composition of the commensal intestinal flora of humans is not static but changes dynamically in response to new environmental conditions...
- A comprehensive analysis of in vitro and in vivo genetic fitness of Pseudomonas aeruginosa using high-throughput sequencing of transposon librariesDavid Skurnik
Division of Infectious Diseases, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, United States of America
PLoS Pathog 9:e1003582. 2013..These experiments resulted in the identification of the P. aeruginosa strain PA14 genes necessary for optimal survival in the mucosal and systemic environments of a mammalian host. ..
- Enhanced in vivo fitness of carbapenem-resistant oprD mutants of Pseudomonas aeruginosa revealed through high-throughput sequencingDavid Skurnik
Division of Infectious Diseases, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115
Proc Natl Acad Sci U S A 110:20747-52. 2013..aeruginosa in infected murine hosts suggests that either drug resistance or host colonization can cause the emergence of more pathogenic, drug-resistant P. aeruginosa clones in a single genetic event. ..
- Identification of Poly-N-acetylglucosamine as a Major Polysaccharide Component of the Bacillus subtilis Biofilm MatrixDamien Roux
From the Division of Infectious Diseases, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, and
J Biol Chem 290:19261-72. 2015..The immunochemical and genetic data identify the genes and proteins used by B. subtilis to produce PNAG as a significant carbohydrate factor essential for pellicle formation. ..
- Magic bullets for the 21st century: the reemergence of immunotherapy for multi- and pan-resistant microbesDamien Roux
Division of Infectious Diseases, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
J Antimicrob Chemother 67:2785-7. 2012..These discoveries could lead to utilization of antibodies to PNAG for either therapeutic use in patients infected by PNAG-producing MDR bacteria or prophylactic use in patients at risk of developing MDR infections...
- Animal and human antibodies to distinct Staphylococcus aureus antigens mutually neutralize opsonic killing and protection in miceDavid Skurnik
Channing Laboratory, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
J Clin Invest 120:3220-33. 2010..aureus infections in humans could be due, in part, to interference in OPK when antibodies to CP and PNAG antigens are both present. This information could be used to better design S. aureus vaccine components...