Priyattam Shiromani

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Hypocretin-2 saporin lesions of the ventrolateral periaquaductal gray (vlPAG) increase REM sleep in hypocretin knockout mice
    Satvinder Kaur
    Veterans Affairs Boston Healthcare System, and Harvard Medical School, West Roxbury, MA, USA
    PLoS ONE 4:e6346. 2009
  2. ncbi request reprint Compensatory sleep response to 12 h wakefulness in young and old rats
    P J Shiromani
    West Roxbury Veterans Affairs Medical Center and Harvard Medical School, West Roxbury 02132, Massachusetts, USA
    Am J Physiol Regul Integr Comp Physiol 278:R125-33. 2000
  3. ncbi request reprint Sleep rhythmicity and homeostasis in mice with targeted disruption of mPeriod genes
    Priyattam J Shiromani
    West Roxbury VA Medical Center, 1400 VFW Parkway, West Roxbury, MA 02132, USA
    Am J Physiol Regul Integr Comp Physiol 287:R47-57. 2004
  4. ncbi request reprint Sleep and wakefulness in c-fos and fos B gene knockout mice
    P J Shiromani
    VA Medical Center and Harvard Medical School, West Roxbury, MA 02132, USA
    Brain Res Mol Brain Res 80:75-87. 2000
  5. pmc The diurnal rhythm of hypocretin in young and old F344 rats
    Frank Desarnaud
    West Roxbury Veterans Affairs Medical Center and Harvard Medical School, Mass 02132, USA
    Sleep 27:851-6. 2004
  6. pmc Effects of saporin-induced lesions of three arousal populations on daily levels of sleep and wake
    Carlos Blanco-Centurion
    West Roxbury Veterans Affairs Medical Center and Harvard Medical School, West Roxbury, Massachusetts 02132, USA
    J Neurosci 27:14041-8. 2007
  7. pmc Effects of hypocretin2-saporin and antidopamine-beta-hydroxylase-saporin neurotoxic lesions of the dorsolateral pons on sleep and muscle tone
    Carlos Blanco-Centurion
    West Roxbury VA Medical Center and Harvard Medical School, 1400 VFW Parkway, West Roxbury, MA 02132, USA
    Eur J Neurosci 19:2741-52. 2004
  8. pmc Effects of hypocretin (orexin) neuronal loss on sleep and extracellular adenosine levels in the rat basal forebrain
    Eric Murillo-Rodriguez
    Department of Neurology, Harvard Medical School, VA Medical Center, West Roxbury, MA 02132, USA
    Eur J Neurosci 28:1191-8. 2008
  9. pmc Activity of pontine neurons during sleep and cataplexy in hypocretin knock-out mice
    Stephen Thankachan
    West Roxbury Veterans Administration Medical Center and Harvard Medical School, West Roxbury, Massachusetts 02132, USA
    J Neurosci 29:1580-5. 2009
  10. pmc Orexin (hypocretin) gene transfer diminishes narcoleptic sleep behavior in mice
    Meng Liu
    West Roxbury Veterans Administration Medical Center and Harvard Medical School, 1400 Veterans of Foreign Wars Parkway, West Roxbury, MA 02132, USA
    Eur J Neurosci 28:1382-93. 2008

Research Grants

  1. BRAIN MECHANISMS IN SLEEP AND NARCOLEPSY
    Priyattam Shiromani; Fiscal Year: 2001
  2. HOMEOSTATIC REGULATION OF SLEEP IN AGING
    Priyattam Shiromani; Fiscal Year: 2002
  3. BRAIN MECHANISMS IN SLEEP AND NARCOLEPSY
    Priyattam Shiromani; Fiscal Year: 2007
  4. BASAL FOREBRAIN HYPOCRETIN REGULATION OF WAKING
    Priyattam Shiromani; Fiscal Year: 2007
  5. HYPOTHALAMIC REGULATION OF SLEEP
    Priyattam Shiromani; Fiscal Year: 2005
  6. BRAINSTEM CHOLINERGIC MECHANISMS IN NARCOLEPSY
    Priyattam Shiromani; Fiscal Year: 1993
  7. BASAL FOREBRAIN HYPOCRETIN REGULATION OF WAKING
    Priyattam J Shiromani; Fiscal Year: 2010
  8. HYPOTHALAMIC REGULATION OF SLEEP
    Priyattam J Shiromani; Fiscal Year: 2010

Collaborators

Detail Information

Publications23

  1. pmc Hypocretin-2 saporin lesions of the ventrolateral periaquaductal gray (vlPAG) increase REM sleep in hypocretin knockout mice
    Satvinder Kaur
    Veterans Affairs Boston Healthcare System, and Harvard Medical School, West Roxbury, MA, USA
    PLoS ONE 4:e6346. 2009
    ..89) with loss of eGFP neurons. These results identify the vlPAG as one site that loses its inhibitory control over REM sleep, but does not cause cataplexy, as a result of hypocretin deficiency...
  2. ncbi request reprint Compensatory sleep response to 12 h wakefulness in young and old rats
    P J Shiromani
    West Roxbury Veterans Affairs Medical Center and Harvard Medical School, West Roxbury 02132, Massachusetts, USA
    Am J Physiol Regul Integr Comp Physiol 278:R125-33. 2000
    ..The findings also indicate that although old rats have a decline in sleep, this cannot be attributed to loss of VLPO neurons implicated in sleep...
  3. ncbi request reprint Sleep rhythmicity and homeostasis in mice with targeted disruption of mPeriod genes
    Priyattam J Shiromani
    West Roxbury VA Medical Center, 1400 VFW Parkway, West Roxbury, MA 02132, USA
    Am J Physiol Regul Integr Comp Physiol 287:R47-57. 2004
    ..Although mPer1 and mPer2 represent key elements of the molecular clock in the SCN, they are not required for homeostatic regulation of the daily amounts of waking, SWS, or REM sleep...
  4. ncbi request reprint Sleep and wakefulness in c-fos and fos B gene knockout mice
    P J Shiromani
    VA Medical Center and Harvard Medical School, West Roxbury, MA 02132, USA
    Brain Res Mol Brain Res 80:75-87. 2000
    ..By demonstrating that these IEGs are involved in sleep, we suggest that the deletion of specific genes, even within a family of genes, can have a specific effect on sleep...
  5. pmc The diurnal rhythm of hypocretin in young and old F344 rats
    Frank Desarnaud
    West Roxbury Veterans Affairs Medical Center and Harvard Medical School, Mass 02132, USA
    Sleep 27:851-6. 2004
    ..In young rats and monkeys, levels of HCRT-1 are highest at the end of the wake-active period and lowest toward the end of the sleep period. However, the effects of age on the diurnal rhythm of HCRT-1 are not known...
  6. pmc Effects of saporin-induced lesions of three arousal populations on daily levels of sleep and wake
    Carlos Blanco-Centurion
    West Roxbury Veterans Affairs Medical Center and Harvard Medical School, West Roxbury, Massachusetts 02132, USA
    J Neurosci 27:14041-8. 2007
    ....
  7. pmc Effects of hypocretin2-saporin and antidopamine-beta-hydroxylase-saporin neurotoxic lesions of the dorsolateral pons on sleep and muscle tone
    Carlos Blanco-Centurion
    West Roxbury VA Medical Center and Harvard Medical School, 1400 VFW Parkway, West Roxbury, MA 02132, USA
    Eur J Neurosci 19:2741-52. 2004
    ....
  8. pmc Effects of hypocretin (orexin) neuronal loss on sleep and extracellular adenosine levels in the rat basal forebrain
    Eric Murillo-Rodriguez
    Department of Neurology, Harvard Medical School, VA Medical Center, West Roxbury, MA 02132, USA
    Eur J Neurosci 28:1191-8. 2008
    ..These findings indicate that adenosine levels do not increase with wakefulness in rats with a HCRT lesion, and that the increased sleep in these rats occurs independently of adenosine levels in the basal forebrain...
  9. pmc Activity of pontine neurons during sleep and cataplexy in hypocretin knock-out mice
    Stephen Thankachan
    West Roxbury Veterans Administration Medical Center and Harvard Medical School, West Roxbury, Massachusetts 02132, USA
    J Neurosci 29:1580-5. 2009
    ..All of these neurons were less active during cataplexy compared with REM sleep. Thus, although cataplexy and REM sleep share many common features, including the muscle atonia, cataplexy is a distinct state in mice...
  10. pmc Orexin (hypocretin) gene transfer diminishes narcoleptic sleep behavior in mice
    Meng Liu
    West Roxbury Veterans Administration Medical Center and Harvard Medical School, 1400 Veterans of Foreign Wars Parkway, West Roxbury, MA 02132, USA
    Eur J Neurosci 28:1382-93. 2008
    ....
  11. pmc Entrainment of temperature and activity rhythms to restricted feeding in orexin knock out mice
    Satvinder Kaur
    West Roxbury VA Medical Center and Harvard Medical School, 1400 VFW Parkway, West Roxbury, MA 02132, USA
    Brain Res 1205:47-54. 2008
    ..We conclude that orexin is not required for entrainment of activity and temperature to a restricted feeding schedule, but is required for the robust expression of gross locomotor activity in anticipation of the scheduled feeding...
  12. ncbi request reprint Effects of inflammation produced by chronic lipopolysaccharide administration on the survival of hypocretin neurons and sleep
    Dmitry Gerashchenko
    West Roxbury VA Medical Center and Harvard Medical School, 1400 VFW Parkway, West Roxbury, MA 02132, USA
    Brain Res 1019:162-9. 2004
    ..The loss of hypocretin neurons produced by chronic LPS administration suggests that inflammation may play a role in the loss of hypocretin neurons in narcolepsy...
  13. ncbi request reprint Different neuronal phenotypes in the lateral hypothalamus and their role in sleep and wakefulness
    Dmitry Gerashchenko
    West Roxbury VA Medical Center and Harvard Medical School, 1400 VFW Parkway, West Roxbury, MA 02132, USA
    Mol Neurobiol 29:41-59. 2004
    ....
  14. ncbi request reprint Relationship between CSF hypocretin levels and hypocretin neuronal loss
    Dmitry Gerashchenko
    West Roxbury VA Medical Center and Harvard Medical School, West Roxbury, MA 02132, USA
    Exp Neurol 184:1010-6. 2003
    ..In those narcoleptics where CSF levels are within the normal range, it is possible that not all of the HCRT neurons are lost and that the surviving HCRT neurons might be increasing output of CSF HCRT...
  15. ncbi request reprint Adenosine and sleep homeostasis in the Basal forebrain
    Carlos Blanco-Centurion
    West Roxbury Veterans Affairs Medical Center and Harvard Medical School, West Roxbury, Massachusetts 02132, USA
    J Neurosci 26:8092-100. 2006
    ..Thus, neither the activity of the BF cholinergic neurons nor the accumulation of AD in the BF during wake is necessary for sleep drive...
  16. ncbi request reprint Effects of hypocretin-1 in 192-IgG-saporin-lesioned rats
    Carlos A Blanco-Centurion
    Department of Neurology, West Roxbury VA Medical Center and Harvard Medical School, 1400 VFW Parkway, West Roxbury, MA 02132, USA
    Eur J Neurosci 24:2084-8. 2006
    ..These results suggest that, in the absence of the basal forebrain cholinergic neurons, the basal forebrain noncholinergic neurons are able to convey hypocretin's arousal signal unabated...
  17. ncbi request reprint Anandamide enhances extracellular levels of adenosine and induces sleep: an in vivo microdialysis study
    Eric Murillo-Rodriguez
    Neurology Department, Harvard Medical School and VA Medical Center, West Roxbury, Mass 02132, USA
    Sleep 26:943-7. 2003
    ..Microdialysis samples were collected from the basal forebrain in order to detect levels of adenosine before and after injection of anandamide...
  18. pmc Effects of lesions of the histaminergic tuberomammillary nucleus on spontaneous sleep in rats
    Dmitry Gerashchenko
    West Roxbury Veterans Affairs Medical Center, West Roxbury, Massachusetts 02132, USA
    Sleep 27:1275-81. 2004
    ..Hence, we placed cell-specific lesions in the TMN to determine its role in spontaneous wakefulness...
  19. ncbi request reprint Effects of hypocaloric diet on sleep in young and old rats
    Rafael J Salin-Pascual
    West Roxbury VA Medical Center and Harvard Medical School, 1400 VFW Parkway, West Roxbury, MA 02132, USA
    Neurobiol Aging 23:771-6. 2002
    ..These results indicate that caloric restriction is unable to prevent the decline in sleep that occurs with aging...
  20. pmc Identification of a population of sleep-active cerebral cortex neurons
    Dmitry Gerashchenko
    Biosciences Division, SRI International, Menlo Park, CA 94025, USA
    Proc Natl Acad Sci U S A 105:10227-32. 2008
    ....
  21. pmc Transplantation of hypocretin neurons into the pontine reticular formation: preliminary results
    Oscar Arias-Carrión
    West Roxbury VA Medical Center and Harvard Medical School, Massachusetts 02132, USA
    Sleep 27:1465-70. 2004
    ..Here we examined the survival of hypocretin-containing neurons grafted into the pontine reticular formation, a region traditionally regarded to be key for rapid eye movement sleep generation...
  22. ncbi request reprint Selective activation of the extended ventrolateral preoptic nucleus during rapid eye movement sleep
    Jun Lu
    Department of Neurology, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    J Neurosci 22:4568-76. 2002
    ..The connections and physiological activity of the extended VLPO suggest a specialized role in the regulation of REM sleep...
  23. ncbi request reprint Caffeine challenge in insomniac patients after total sleep deprivation
    Rafael J Salin-Pascual
    Departamento de Fisiologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Mexico
    Sleep Med 7:141-5. 2006
    ..We hypothesized that insomniacs would be more affected by caffeine, which would suggest a change in adenosine receptor (number or sensitivity) in primary insomniacs...

Research Grants34

  1. BRAIN MECHANISMS IN SLEEP AND NARCOLEPSY
    Priyattam Shiromani; Fiscal Year: 2001
    ..The PI's studies to date have used c-Fos to identify the neuronal mechanisms generating sleep. To the best of our knowledge no current program is examining transcription factors as playing a role in the disease. ..
  2. HOMEOSTATIC REGULATION OF SLEEP IN AGING
    Priyattam Shiromani; Fiscal Year: 2002
    ..Their preliminary results indicate that somnogens such as adenosine induce c-Fos (measured using immunohistochemistry and on tissue slices, and via western blot analysis of basal forebrain tissue homogenate) and AP-1. ..
  3. BRAIN MECHANISMS IN SLEEP AND NARCOLEPSY
    Priyattam Shiromani; Fiscal Year: 2007
    ..Specific aim 3 will test the hypothesis that loss of a specific population of HCRT neurons is responsible for the symptoms of narcolepsy. Specific aim 4 will determine the afferents and efferents of this population of HCRT neurons. ..
  4. BASAL FOREBRAIN HYPOCRETIN REGULATION OF WAKING
    Priyattam Shiromani; Fiscal Year: 2007
    ..Then 192-lgG saporin will be used in the transgenic rats to lesion the BF cholinergic neurons and we hypothesize that with both the BF cholinergic and HCRT neurons lost, the rats should have more sleep compared to single lesions. ..
  5. HYPOTHALAMIC REGULATION OF SLEEP
    Priyattam Shiromani; Fiscal Year: 2005
    ..This will determine which receptor subtype containing neurons are affected by the saporin conjugate. Our findings will provide a framework for integrating the hypocretin/orexin cells within an overall model of sleep regulation. ..
  6. BRAINSTEM CHOLINERGIC MECHANISMS IN NARCOLEPSY
    Priyattam Shiromani; Fiscal Year: 1993
    ..The protein might act as a third messenger serving to regulate the functional response of the cell. These studies will establish a groundwork for future work related to the transcriptional processes occuring during REM sleep...
  7. BASAL FOREBRAIN HYPOCRETIN REGULATION OF WAKING
    Priyattam J Shiromani; Fiscal Year: 2010
    ..abstract_text> ..
  8. HYPOTHALAMIC REGULATION OF SLEEP
    Priyattam J Shiromani; Fiscal Year: 2010
    ..The significance of this project is that it will develop a gene transfer approach that will serve as a neurobiological tool to understand the networking underlying narcolepsy and also to ultimately restore some function. ..