William M Shih

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Folding DNA origami from a double-stranded source of scaffold
    Björn Högberg
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Am Chem Soc 131:9154-5. 2009
  2. pmc Knitting complex weaves with DNA origami
    William M Shih
    Department of Cancer Biology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Curr Opin Struct Biol 20:276-82. 2010
  3. pmc Self-assembly of three-dimensional prestressed tensegrity structures from DNA
    Tim Liedl
    Department of Cancer Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Nanotechnol 5:520-4. 2010
  4. pmc Self-assembly of DNA into nanoscale three-dimensional shapes
    Shawn M Douglas
    Department of Cancer Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 459:414-8. 2009
  5. pmc Purification of DNA-origami nanostructures by rate-zonal centrifugation
    Chenxiang Lin
    Department of Cancer Biology, Dana Farber Cancer Institute, Harvard University, Boston, MA 02115, USA
    Nucleic Acids Res 41:e40. 2013
  6. pmc Folding DNA into twisted and curved nanoscale shapes
    Hendrik Dietz
    Department of Cancer Biology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 325:725-30. 2009
  7. pmc Three-dimensional structures self-assembled from DNA bricks
    Yonggang Ke
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA
    Science 338:1177-83. 2012
  8. pmc Rapid prototyping of 3D DNA-origami shapes with caDNAno
    Shawn M Douglas
    Department of Cancer Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Nucleic Acids Res 37:5001-6. 2009
  9. pmc Submicrometre geometrically encoded fluorescent barcodes self-assembled from DNA
    Chenxiang Lin
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA
    Nat Chem 4:832-9. 2012
  10. pmc DNA nanotubes for NMR structure determination of membrane proteins
    Gaëtan Bellot
    1 Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA 2 Department of Cancer Biology, Dana Farber Cancer Institute, Boston, Massachusetts, USA 3 Wyss Institute for Biologically Inspired Engineering at Harvard, Boston, Massachusetts, USA
    Nat Protoc 8:755-70. 2013

Collaborators

Detail Information

Publications16

  1. pmc Folding DNA origami from a double-stranded source of scaffold
    Björn Högberg
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Am Chem Soc 131:9154-5. 2009
    ....
  2. pmc Knitting complex weaves with DNA origami
    William M Shih
    Department of Cancer Biology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Curr Opin Struct Biol 20:276-82. 2010
    ..Today, two-dimensional and three-dimensional custom-shaped nanostructures comparable in mass to a small virus can be designed, assembled, and characterized with a prototyping cycle on the order of a couple of weeks...
  3. pmc Self-assembly of three-dimensional prestressed tensegrity structures from DNA
    Tim Liedl
    Department of Cancer Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Nanotechnol 5:520-4. 2010
    ..In addition to being building blocks for nanostructures, tensile structural elements made of single-stranded DNA could be used to study molecular forces, cellular mechanotransduction and other fundamental biological processes...
  4. pmc Self-assembly of DNA into nanoscale three-dimensional shapes
    Shawn M Douglas
    Department of Cancer Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 459:414-8. 2009
    ..We anticipate that our strategy for self-assembling custom three-dimensional shapes will provide a general route to the manufacture of sophisticated devices bearing features on the nanometre scale...
  5. pmc Purification of DNA-origami nanostructures by rate-zonal centrifugation
    Chenxiang Lin
    Department of Cancer Biology, Dana Farber Cancer Institute, Harvard University, Boston, MA 02115, USA
    Nucleic Acids Res 41:e40. 2013
    ..g. milligram quantities) of DNA nanostructures...
  6. pmc Folding DNA into twisted and curved nanoscale shapes
    Hendrik Dietz
    Department of Cancer Biology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 325:725-30. 2009
    ..We also combined multiple curved elements to build several different types of intricate nanostructures, such as a wireframe beach ball or square-toothed gears...
  7. pmc Three-dimensional structures self-assembled from DNA bricks
    Yonggang Ke
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA
    Science 338:1177-83. 2012
    ..By selecting subsets of bricks from this canvas, we constructed a panel of 102 distinct shapes exhibiting sophisticated surface features, as well as intricate interior cavities and tunnels...
  8. pmc Rapid prototyping of 3D DNA-origami shapes with caDNAno
    Shawn M Douglas
    Department of Cancer Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Nucleic Acids Res 37:5001-6. 2009
    ..The software is available at http://cadnano.org/, along with example designs and video tutorials demonstrating their construction. The source code is released under the MIT license...
  9. pmc Submicrometre geometrically encoded fluorescent barcodes self-assembled from DNA
    Chenxiang Lin
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA
    Nat Chem 4:832-9. 2012
    ..One species of the barcodes was used to tag yeast surface receptors, which suggests their potential applications as in situ imaging probes for diverse biomolecular and cellular entities in their native environments...
  10. pmc DNA nanotubes for NMR structure determination of membrane proteins
    Gaëtan Bellot
    1 Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA 2 Department of Cancer Biology, Dana Farber Cancer Institute, Boston, Massachusetts, USA 3 Wyss Institute for Biologically Inspired Engineering at Harvard, Boston, Massachusetts, USA
    Nat Protoc 8:755-70. 2013
    ..Production of sufficient nanotubes for four or five NMR experiments can be completed in 1 week by a single individual...
  11. pmc Multilayer DNA origami packed on hexagonal and hybrid lattices
    Yonggang Ke
    Department of Cancer Biology, Dana Farber Cancer Institute, and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Am Chem Soc 134:1770-4. 2012
    ..The availability of hexagonal close-packing of helices extends our ability to build complex structures using DNA nanotechnology...
  12. pmc Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT
    Ralf Jungmann
    1 Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA 2 Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA 3
    Nat Methods 11:313-8. 2014
    ..We experimentally demonstrate ten-color super-resolution imaging in vitro on synthetic DNA structures as well as four-color two-dimensional (2D) imaging and three-color 3D imaging of proteins in fixed cells. ..
  13. ncbi request reprint DNA nanotubes for NMR structure determination of membrane proteins
    Gaëtan Bellot
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA
    Nat Protoc 8:755-70. 2013
    ..Production of sufficient nanotubes for four or five NMR experiments can be completed in 1 week by a single individual...
  14. pmc Virus-inspired membrane encapsulation of DNA nanostructures to achieve in vivo stability
    Steven D Perrault
    Wyss Institute for Biologically Inspired Engineering and Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, United States
    ACS Nano 8:5132-40. 2014
    ..By establishing a design strategy suitable for biomedical applications, we have provided a platform for the engineering of sophisticated, translation-ready DNA nanodevices. ..
  15. pmc Mitochondrial uncoupling protein 2 structure determined by NMR molecular fragment searching
    Marcelo J Berardi
    Jack and Eileen Connors Structural Biology Laboratory, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 476:109-13. 2011
    ....
  16. pmc DNA-nanotube-induced alignment of membrane proteins for NMR structure determination
    Shawn M Douglas
    Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:6644-8. 2007
    ..We anticipate that this medium will extend the advantages of weak alignment to NMR structure determination of a broad range of detergent-solubilized membrane proteins...