Alexander Schier

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Attenuation of Notch and Hedgehog signaling is required for fate specification in the spinal cord
    Peng Huang
    Department of Molecular and Cellular Biology, Center for Brain Science, Harvard Stem Cell Institute, Broad Institute, Center for Systems Biology, Harvard University, Cambridge, Massachusetts, USA
    PLoS Genet 8:e1002762. 2012
  2. doi request reprint Should I stay or should I go: neuromodulators of behavioral states
    Alexander F Schier
    Department of Molecular and Cellular Biology, Center for Brain Science, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Cell 154:955-6. 2013
  3. pmc Touch responsiveness in zebrafish requires voltage-gated calcium channel 2.1b
    Sean E Low
    Départment de Pathologie et Biologie Cellulaire, Groupe de Recherche sur le Système Nerveux Central et Centre d Excellence en Neuromique de l Université de Montéral, Universite de Montreal, Montreal, Quebec, Canada
    J Neurophysiol 108:148-59. 2012
  4. ncbi request reprint Axis formation: squint comes into focus
    Alexander F Schier
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Curr Biol 15:R1002-5. 2005
  5. ncbi request reprint The maternal-zygotic transition: death and birth of RNAs
    Alexander F Schier
    Department of Molecular and Cellular Biology, Harvard Stem Cell Institute, Center for Brain Science, Broad Institute, Harvard University, 16 Divinity Avenue, Room 1027, Cambridge, MA 02138, USA
    Science 316:406-7. 2007
  6. ncbi request reprint Target protectors reveal dampening and balancing of Nodal agonist and antagonist by miR-430
    Wen Yee Choi
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Science 318:271-4. 2007
  7. pmc Monitoring neural activity with bioluminescence during natural behavior
    Eva A Naumann
    Department of Molecular and Cellular Biology, Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA
    Nat Neurosci 13:513-20. 2010
  8. pmc Chromatin signature of embryonic pluripotency is established during genome activation
    Nadine L Vastenhouw
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Nature 464:922-6. 2010
  9. pmc In vivo birthdating by BAPTISM reveals that trigeminal sensory neuron diversity depends on early neurogenesis
    Sophie J C Caron
    Department of Molecular and Cellular Biology, Center for Brain Science, Broad Institute, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA
    Development 135:3259-69. 2008
  10. pmc Members of the miRNA-200 family regulate olfactory neurogenesis
    Philip S Choi
    Howard Hughes Medical Institute, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Neuron 57:41-55. 2008

Research Grants

  1. TARGETED MANIPULATION OF THE ZEBRAFISH GENOME
    Alexander Schier; Fiscal Year: 2005
  2. 2007/2009 "Developmental Biology" Gordon Research Conferences
    Alexander Schier; Fiscal Year: 2007
  3. COMPARATIVE GENETICS OF GLI FUNCTION
    Alexander Schier; Fiscal Year: 2007
  4. MOLECULAR GENETICS OF SENSORY GANGLIA DEVELOPMENT
    Alexander Schier; Fiscal Year: 2007
  5. GENETIC MECHANISMS OF AXIS FORMATION IN VERTEBRATES
    Alexander Schier; Fiscal Year: 2009
  6. Genetics of Sleep in Zebrafish
    Alexander F Schier; Fiscal Year: 2010
  7. GENETIC MECHANISMS OF AXIS FORMATION IN VERTEBRATES
    Alexander Schier; Fiscal Year: 2009
  8. GENETIC MECHANISMS OF AXIS FORMATION IN VERTEBRATES
    Alexander Schier; Fiscal Year: 2005
  9. GENETIC MECHANISMS OF AXIS FORMATION IN VERTEBRATES
    Alexander Schier; Fiscal Year: 2001
  10. MOLECULAR GENETICS OF FOREBRAIN DEVELOPMENT
    Alexander Schier; Fiscal Year: 2003

Collaborators

Detail Information

Publications47

  1. pmc Attenuation of Notch and Hedgehog signaling is required for fate specification in the spinal cord
    Peng Huang
    Department of Molecular and Cellular Biology, Center for Brain Science, Harvard Stem Cell Institute, Broad Institute, Center for Systems Biology, Harvard University, Cambridge, Massachusetts, USA
    PLoS Genet 8:e1002762. 2012
    ....
  2. doi request reprint Should I stay or should I go: neuromodulators of behavioral states
    Alexander F Schier
    Department of Molecular and Cellular Biology, Center for Brain Science, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Cell 154:955-6. 2013
    ..Flavell et al. now identify molecular and neural components in C. elegans that are involved in the generation of dwelling and roaming states. ..
  3. pmc Touch responsiveness in zebrafish requires voltage-gated calcium channel 2.1b
    Sean E Low
    Départment de Pathologie et Biologie Cellulaire, Groupe de Recherche sur le Système Nerveux Central et Centre d Excellence en Neuromique de l Université de Montéral, Universite de Montreal, Montreal, Quebec, Canada
    J Neurophysiol 108:148-59. 2012
    ..These results, coupled with the expression of CACNA1Ab by sensory neurons, suggest that CaV2.1b channel activity is necessary for touch-evoked activation of the locomotor network in zebrafish...
  4. ncbi request reprint Axis formation: squint comes into focus
    Alexander F Schier
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Curr Biol 15:R1002-5. 2005
    ..Gene products provided by the mother to the embryo determine the body axes in most animals. A recent study in zebrafish proposes that the TGFss signal Squint is one such factor...
  5. ncbi request reprint The maternal-zygotic transition: death and birth of RNAs
    Alexander F Schier
    Department of Molecular and Cellular Biology, Harvard Stem Cell Institute, Center for Brain Science, Broad Institute, Harvard University, 16 Divinity Avenue, Room 1027, Cambridge, MA 02138, USA
    Science 316:406-7. 2007
    ..Maternal RNA degradation is induced by the binding of proteins and microRNAs to the 3' untranslated region of target RNAs...
  6. ncbi request reprint Target protectors reveal dampening and balancing of Nodal agonist and antagonist by miR-430
    Wen Yee Choi
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Science 318:271-4. 2007
    ..These findings establish an approach to analyze the in vivo roles of specific miRNA-mRNA pairs and reveal a requirement for miRNAs in dampening and balancing agonist/antagonist pairs...
  7. pmc Monitoring neural activity with bioluminescence during natural behavior
    Eva A Naumann
    Department of Molecular and Cellular Biology, Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA
    Nat Neurosci 13:513-20. 2010
    ..Our neuroluminescence assay can report, with high temporal resolution and sensitivity, the activity of small subsets of neurons during unrestrained behavior...
  8. pmc Chromatin signature of embryonic pluripotency is established during genome activation
    Nadine L Vastenhouw
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Nature 464:922-6. 2010
    ..These results indicate that bivalent and monovalent domains might poise embryonic genes for activation and that the chromatin profile associated with pluripotency is established during the maternal-zygotic transition...
  9. pmc In vivo birthdating by BAPTISM reveals that trigeminal sensory neuron diversity depends on early neurogenesis
    Sophie J C Caron
    Department of Molecular and Cellular Biology, Center for Brain Science, Broad Institute, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA
    Development 135:3259-69. 2008
    ..These results indicate that the multimodal specification and function of trigeminal sensory ganglia depends on the timing of neurogenesis...
  10. pmc Members of the miRNA-200 family regulate olfactory neurogenesis
    Philip S Choi
    Howard Hughes Medical Institute, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Neuron 57:41-55. 2008
    ....
  11. doi request reprint Non-coding RNAs as regulators of embryogenesis
    Andrea Pauli
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nat Rev Genet 12:136-49. 2011
    ..These examples also highlight the roles of ncRNAs in ensuring a robust commitment to one of two possible cell fates...
  12. ncbi request reprint Zebrafish MiR-430 promotes deadenylation and clearance of maternal mRNAs
    Antonio J Giraldez
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
    Science 312:75-9. 2006
    ..We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis...
  13. ncbi request reprint Hypocretin/orexin overexpression induces an insomnia-like phenotype in zebrafish
    David A Prober
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Neurosci 26:13400-10. 2006
    ....
  14. pmc Targeted mutagenesis in zebrafish
    Ian G Woods
    Department of Molecular and Cellular Biology, Broad Institute, Center for Brain Science, Harvard Stem Cell Institute, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Nat Biotechnol 26:650-1. 2008
  15. pmc Dampened Hedgehog signaling but normal Wnt signaling in zebrafish without cilia
    Peng Huang
    Department of Molecular and Cellular Biology, Center for Brain Science, Harvard Stem Cell Institute, Broad Institute, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Development 136:3089-98. 2009
    ..These results reveal a conserved requirement for cilia in transducing the activity of upstream regulators of Hh signaling but distinct phenotypic effects due to differential regulation and differing roles of transcriptional mediators...
  16. pmc Nodal morphogens
    Alexander F Schier
    Department of Molecular and Cellular Biology, Harvard Stem Cell Institute, Center for Brain Science, Broad Institute, Harvard University, Cambridge, Massachusetts 02138, USA
    Cold Spring Harb Perspect Biol 1:a003459. 2009
    ..This article describes how Nodal morphogens pattern embryonic fields and discusses how Nodal morphogen signaling is modulated...
  17. pmc Zebrafish behavioral profiling links drugs to biological targets and rest/wake regulation
    Jason Rihel
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Science 327:348-51. 2010
    ..These results demonstrate the power of high-throughput behavioral profiling in zebrafish to discover and characterize psychotropic drugs and to dissect the pharmacology of complex behaviors...
  18. pmc Zebrafish TRPA1 channels are required for chemosensation but not for thermosensation or mechanosensory hair cell function
    David A Prober
    Department of Molecular and Cellular Biology, Center for Brain Science, Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts 02138, USA
    J Neurosci 28:10102-10. 2008
    ....
  19. pmc Escape behavior elicited by single, channelrhodopsin-2-evoked spikes in zebrafish somatosensory neurons
    Adam D Douglass
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Curr Biol 18:1133-7. 2008
    ..These results establish ChR2 as a powerful tool for the manipulation of neural activity in zebrafish and reveal a degree of efficiency in coding that has not been found in primary sensory neurons...
  20. pmc Nodal signaling promotes the speed and directional movement of cardiomyocytes in zebrafish
    Maria Ines Medeiros de Campos-Baptista
    Department of Molecular and Cellular Biology, Center for Brain Science, Broad Institute, Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts 02138, USA
    Dev Dyn 237:3624-33. 2008
    ..These results indicate that a Nodal-BMP signaling cascade drives left-right heart morphogenesis by regulating the speed and direction of cardiomyocyte movement...
  21. ncbi request reprint Cilia-driven fluid flow in the zebrafish pronephros, brain and Kupffer's vesicle is required for normal organogenesis
    Albrecht G Kramer-Zucker
    Renal Unit, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 02129, USA
    Development 132:1907-21. 2005
    ..In Kupffer's vesicle, loss of flow is associated with loss of left-right patterning, indicating that the 'nodal flow' mechanism of generating situs is conserved in non-mammalian vertebrates...
  22. ncbi request reprint Stat3 Controls Cell Movements during Zebrafish Gastrulation
    Susumu Yamashita
    Department of Molecular Oncology C 7, Osaka University Graduate School of Medicine, 2 2 Yamada oka, 565 0871, Suita Osaka, Japan
    Dev Cell 2:363-75. 2002
    ..These results reveal a role for Stat3 in controlling cell movements during gastrulation...
  23. ncbi request reprint Mixer/Bon and FoxH1/Sur have overlapping and divergent roles in Nodal signaling and mesendoderm induction
    Prabhat S Kunwar
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
    Development 130:5589-99. 2003
    ....
  24. ncbi request reprint The role of the zebrafish nodal-related genes squint and cyclops in patterning of mesendoderm
    Scott T Dougan
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Development 130:1837-51. 2003
    ..In addition, the differential timing of dorsal mesendoderm induction in squint and cyclops mutants suggests that dorsal marginal cells can respond to Nodal signals at stages ranging from the mid-blastula through the mid-gastrula...
  25. ncbi request reprint Genetic analysis of zebrafish gli1 and gli2 reveals divergent requirements for gli genes in vertebrate development
    Rolf O Karlstrom
    Department of Biology, University of Massachusetts, Amherst, MA 01003, USA
    Development 130:1549-64. 2003
    ..These results reveal divergent requirements for Gli1 and Gli2 in mouse and zebrafish and indicate that zebrafish Gli1 is an activator of Hh-regulated genes, while zebrafish Gli2 has minor roles as a repressor or activator of Hh targets...
  26. pmc EGF-CFC proteins are essential coreceptors for the TGF-beta signals Vg1 and GDF1
    Simon K Cheng
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, New York 10016, USA
    Genes Dev 17:31-6. 2003
    ..These results establish that multiple TGF-beta signals converge on Activin receptor/EGF-CFC complexes and suggest a more widespread requirement for coreceptors in TGF-beta signaling than anticipated previously...
  27. ncbi request reprint Lefty proteins are long-range inhibitors of squint-mediated nodal signaling
    Yu Chen
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, New York 10016, USA
    Curr Biol 12:2124-8. 2002
    ..We also find that Lefty restricts the response to both high and low levels of Nodal signaling. These results indicate that Lefty proteins restrict the activity range of Nodal signals by dampening Nodal signaling in surrounding cells...
  28. ncbi request reprint Maternal nodal and zebrafish embryogenesis
    James T Bennett
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York 10016, USA
    Nature 450:E1-2; discussion E2-4. 2007
    ..Here we test their proposal and show that the maternal activities of sqt and the related Nodal gene cyclops (cyc) are not required for dorsoventral patterning...
  29. ncbi request reprint Repulsive interactions shape the morphologies and functional arrangement of zebrafish peripheral sensory arbors
    Alvaro Sagasti
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine and Department of Cell Biology, New York University School of Medicine, New York, New York 10016, USA
    Curr Biol 15:804-14. 2005
    ..We investigated the developmental mechanisms that determine the size and shape of individual trigeminal arbors in zebrafish and analyzed how these interactions affect the functional organization of the peripheral sensory system...
  30. ncbi request reprint MicroRNAs regulate brain morphogenesis in zebrafish
    Antonio J Giraldez
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine and Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
    Science 308:833-8. 2005
    ..Injection of miR-430 miRNAs rescues the brain defects in MZdicer mutants, revealing essential roles for miRNAs during morphogenesis...
  31. ncbi request reprint Assembly of trigeminal sensory ganglia by chemokine signaling
    Holger Knaut
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, New York University School of Medicine, New York, New York 10016, USA
    Neuron 47:653-66. 2005
    ..These results indicate that ganglion formation depends on the interplay of birthplace, chemokine attraction, cell-cell interaction, and cadherin-mediated adhesion...
  32. pmc Planar cell polarity signalling couples cell division and morphogenesis during neurulation
    Brian Ciruna
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine and Department of Cell Biology, New York University School of Medicine, New York, New York 10016, USA
    Nature 439:220-4. 2006
    ..These results reveal a function for PCP signalling in coupling cell division and morphogenesis at neurulation and indicate a previously unrecognized mechanism that might underlie NTDs...
  33. pmc Polycystin-2 immunolocalization and function in zebrafish
    Tomoko Obara
    Nephrology Division, Massachusetts General Hospital 149 8000 and Harvard Medical School, 149 13th Street, Charlestown, MA 02129, USA
    J Am Soc Nephrol 17:2706-18. 2006
    ....
  34. pmc Differential regulation of germline mRNAs in soma and germ cells by zebrafish miR-430
    Yuichiro Mishima
    Department of Biology, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nadaku, Kobe 657 8501, Japan
    Curr Biol 16:2135-42. 2006
    ..These results indicate that miR-430 targets the 3' UTRs of germline genes and suggest that differential susceptibility to microRNAs contributes to tissue-specific gene expression...
  35. ncbi request reprint Zebrafish Gli3 functions as both an activator and a repressor in Hedgehog signaling
    Oksana V Tyurina
    Department of Biology, University of Massachusetts, Amherst, MA 01003 9297, USA
    Dev Biol 277:537-56. 2005
    ..These results reveal a conserved role for Gli3 in vertebrate development and uncover novel regional functions and regulatory interactions among gli genes...
  36. pmc Nodal signaling activates differentiation genes during zebrafish gastrulation
    James T Bennett
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
    Dev Biol 304:525-40. 2007
    ..These results indicate that Nodal signaling regulates not only specification genes but also differentiation genes...
  37. ncbi request reprint Molecular genetics of axis formation in zebrafish
    Alexander F Schier
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016 6497, USA
    Annu Rev Genet 39:561-613. 2005
    ..Noncanonical Wnt signaling is required for the morphogenetic movements during gastrulation. We review how the coordinated interplay of these molecules determines the fate and movement of embryonic cells...
  38. ncbi request reprint A novel microtubule destabilizing entity from orthogonal synthesis of triazine library and zebrafish embryo screening
    Ho Sang Moon
    Department of Chemistry, New York University, New York, NY 10003, USA
    J Am Chem Soc 124:11608-9. 2002
    ..Novel triazine-based microtubule inhibitors were discovered by an efficient zebrafish embryo screening and in vitro microtubule polymerization assay...
  39. ncbi request reprint Mesoderm induction and patterning
    David Kimelman
    Department of Biochemistry, Box 357350, University of Washington, Seattle, Washington 98195 7350, USA
    Results Probl Cell Differ 40:15-27. 2002
  40. ncbi request reprint A loss-of-function mutation in the CFC domain of TDGF1 is associated with human forebrain defects
    June M de la Cruz
    Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, 10 Center Drive, MSC 1852, Building 10 Room 10C103, Bethesda, MD 20892 1852, USA
    Hum Genet 110:422-8. 2002
    ..The mutant protein is inactive in a zebrafish rescue assay, indicating a role for TDGF1 in human midline and forebrain development...
  41. pmc Production of maternal-zygotic mutant zebrafish by germ-line replacement
    Brian Ciruna
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, New York University School of Medicine, New York 10016, USA
    Proc Natl Acad Sci U S A 99:14919-24. 2002
    ..Furthermore, the ability to generate large clutches of purely mutant embryos will greatly facilitate embryological, genetic, genomic, and biochemical studies...
  42. ncbi request reprint Chemokine signaling: rules of attraction
    Alexander F Schier
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
    Curr Biol 13:R192-4. 2003
    ..The chemokine SDF-1 and its receptor CXCR4 control cell migration in the immune and nervous systems. Recent studies in zebrafish have shown that SDF-1 and CXCR4 also guide the migration of germ cells and sensory organs of the lateral line...
  43. pmc Lefty blocks a subset of TGFbeta signals by antagonizing EGF-CFC coreceptors
    Simon K Cheng
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, New York University School of Medicine, New York, USA
    PLoS Biol 2:E30. 2004
    ..These results indicate that coreceptors are targets for both TGFbeta agonists and antagonists and suggest that subtle sequence variations in TGFbeta signals result in greater ligand diversity...
  44. ncbi request reprint Nodal stability determines signaling range
    J Ann Le Good
    Swiss Institute for Experimental Cancer Research ISREC, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Curr Biol 15:31-6. 2005
    ..These results suggest that increased proteolytic maturation of Nodal potentiates autocrine signaling, whereas increased Nodal stability extends paracrine signaling...
  45. ncbi request reprint Nodal signaling in vertebrate development
    Alexander F Schier
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Annu Rev Cell Dev Biol 19:589-621. 2003
    ..These studies demonstrate that Nodal signaling is modulated at almost every level to precisely orchestrate tissue patterning during vertebrate embryogenesis...
  46. ncbi request reprint Developmental biology: tail of decay
    Alexander F Schier
    Nature 427:403-4. 2004
  47. doi request reprint Clearing the path for germ cells
    Holger Knaut
    Developmental Genetics Program, Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA
    Cell 132:337-9. 2008
    ..These results highlight the importance of ligand clearance during guided cell migration...

Research Grants37

  1. TARGETED MANIPULATION OF THE ZEBRAFISH GENOME
    Alexander Schier; Fiscal Year: 2005
    ..We will test both positive and positive/negative selection protocols. Based on the results of vasa targeting, we will then target the no tail gene. ..
  2. 2007/2009 "Developmental Biology" Gordon Research Conferences
    Alexander Schier; Fiscal Year: 2007
    ..By maximizing both formal discussion and informal interactions the Gordon Conference on Developmental Biology will help to define both the present state and future of the field. ..
  3. COMPARATIVE GENETICS OF GLI FUNCTION
    Alexander Schier; Fiscal Year: 2007
    ..These comparative genetic studies will allow detailed insights into the conservation and divergence of orthologous genes and will test the value of animal models in understanding human birth defects. ..
  4. MOLECULAR GENETICS OF SENSORY GANGLIA DEVELOPMENT
    Alexander Schier; Fiscal Year: 2007
    ..These studies will contribute to our understanding of ganglion development and provide insights into the mechanisms through which neurons assemble and partition receptive areas, two fundamental issues in developmental neurobiology. ..
  5. GENETIC MECHANISMS OF AXIS FORMATION IN VERTEBRATES
    Alexander Schier; Fiscal Year: 2009
    ..The proposed studies will thus help to inform strategies to induce stem cells to differentiate into organ progenitors. ..
  6. Genetics of Sleep in Zebrafish
    Alexander F Schier; Fiscal Year: 2010
    ..The discovery of genes that regulate sleep in zebrafish will help to uncover the molecular underpinnings of sleep and inform strategies for the treatment of sleep disorders. ..
  7. GENETIC MECHANISMS OF AXIS FORMATION IN VERTEBRATES
    Alexander Schier; Fiscal Year: 2009
    ..The in vitro generation of endoderm or mesoderm is crucial to develop cell-based therapies for regenerative medicine. The proposed studies will thus help to inform strategies to induce stem cells to differentiate into organ progenitors. ..
  8. GENETIC MECHANISMS OF AXIS FORMATION IN VERTEBRATES
    Alexander Schier; Fiscal Year: 2005
    ..Mutations in EGF-CFC genes in humans are associated with forebrain and left-right defects. The proposed studies will thus help to provide the necessary context for understanding these developmental abnormalities. ..
  9. GENETIC MECHANISMS OF AXIS FORMATION IN VERTEBRATES
    Alexander Schier; Fiscal Year: 2001
    ..Additional alleles will be generated at the loci with further phenotypic characterization and epistatic relationships to oep being established. Also the mutations will be placed on the zebrafish genetic map. ..
  10. MOLECULAR GENETICS OF FOREBRAIN DEVELOPMENT
    Alexander Schier; Fiscal Year: 2003
    ..The proposed studies will thus help to provide the necessary context for understanding birth defects and cancer formation in humans. ..
  11. SENSITIZED SCREEN MUTATION AFFECTING EARLY EMBRYOGENESIS
    Alexander Schier; Fiscal Year: 2003
    ..Modulating loci will be identified by detailed phenotypic analysis. This sensitized screen will provide important resources and fundamental insights into developmental signaling pathways in vertebrates. ..
  12. Multicolor Fluorescent Analysis in Zebrafish
    Alexander F Schier; Fiscal Year: 2010
    ..The proposed work is to enhance the utility of this model system by developing tools to label cells in multiple different colors and follow their development. ..