David E Saslowsky

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Ganglioside GM1-mediated transcytosis of cholera toxin bypasses the retrograde pathway and depends on the structure of the ceramide domain
    David E Saslowsky
    Division of Gastroenterology, Boston Children s Hospital, Boston, Massachusetts 02115, USA
    J Biol Chem 288:25804-9. 2013
  2. ncbi request reprint Conversion of apical plasma membrane sphingomyelin to ceramide attenuates the intoxication of host cells by cholera toxin
    David E Saslowsky
    Children s Hospital, Harvard Digestive Diseases Center, Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Cell Microbiol 10:67-80. 2008
  3. pmc Ceramide activates JNK to inhibit a cAMP-gated K+ conductance and Cl- secretion in intestinal epithelia
    David E Saslowsky
    GI Cell Biology, Children s Hospital, and the Harvard Digestive Diseases Center, Boston, MA 02115, USA
    FASEB J 23:259-70. 2009
  4. pmc Lipid sorting by ceramide structure from plasma membrane to ER for the cholera toxin receptor ganglioside GM1
    Daniel J F Chinnapen
    Division of Gastroenterology, Boston Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 23:573-86. 2012
  5. pmc Intoxication of zebrafish and mammalian cells by cholera toxin depends on the flotillin/reggie proteins but not Derlin-1 or -2
    David E Saslowsky
    Division of Gastroenterology, Children s Hospital, Boston, Massachusetts, USA
    J Clin Invest 120:4399-4409. 2010
  6. pmc Attenuated endocytosis and toxicity of a mutant cholera toxin with decreased ability to cluster ganglioside GM1 molecules
    Anne A Wolf
    GI Cell Biology, Enders 720, Children s Hospital Boston, 300 Longwood Ave, Boston, MA 02115, USA
    Infect Immun 76:1476-84. 2008
  7. ncbi request reprint Placental alkaline phosphatase is efficiently targeted to rafts in supported lipid bilayers
    David E Saslowsky
    Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
    J Biol Chem 277:26966-70. 2002
  8. pmc Real-time analysis of the effects of cholesterol on lipid raft behavior using atomic force microscopy
    Jared C Lawrence
    Department of Pharmacology, University of Cambridge, United Kingdom
    Biophys J 84:1827-32. 2003

Research Grants

Collaborators

  • Wayne I Lencer
  • Anne K Kenworthy
  • Tobias Baumgart
  • Daniel J F Chinnapen
  • Kimberly R Drake
  • Anne A Wolf
  • Jared C Lawrence
  • Catherine E Costello
  • Jessica S Wagner
  • Eelke Brandsma
  • Lydia Kaoutzani
  • Yvonne M te Welscher
  • M David Ullman
  • Minchul Kang
  • Thomas Benjamin
  • Hyejung Park
  • Ramiro H Massol
  • Ludovic D'auria
  • Wan Ting Hsieh
  • Eli Kern
  • Michael G Jobling
  • Randall K Holmes
  • J Michael Edwardson
  • Robert M Henderson

Detail Information

Publications8

  1. pmc Ganglioside GM1-mediated transcytosis of cholera toxin bypasses the retrograde pathway and depends on the structure of the ceramide domain
    David E Saslowsky
    Division of Gastroenterology, Boston Children s Hospital, Boston, Massachusetts 02115, USA
    J Biol Chem 288:25804-9. 2013
    ....
  2. ncbi request reprint Conversion of apical plasma membrane sphingomyelin to ceramide attenuates the intoxication of host cells by cholera toxin
    David E Saslowsky
    Children s Hospital, Harvard Digestive Diseases Center, Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Cell Microbiol 10:67-80. 2008
    ..This result suggests that the cause for toxin resistance occurs at steps required for retro-translocation of the CT A1-chain to the cytosol...
  3. pmc Ceramide activates JNK to inhibit a cAMP-gated K+ conductance and Cl- secretion in intestinal epithelia
    David E Saslowsky
    GI Cell Biology, Children s Hospital, and the Harvard Digestive Diseases Center, Boston, MA 02115, USA
    FASEB J 23:259-70. 2009
    ..These results define a novel lipid-mediated pathway for regulation of salt and water homeostasis at mucosal surfaces...
  4. pmc Lipid sorting by ceramide structure from plasma membrane to ER for the cholera toxin receptor ganglioside GM1
    Daniel J F Chinnapen
    Division of Gastroenterology, Boston Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 23:573-86. 2012
    ..The results implicate a protein-dependent mechanism of lipid sorting by ceramide structure and provide a molecular explanation for the diversity and specificity of retrograde trafficking by CT in host cells...
  5. pmc Intoxication of zebrafish and mammalian cells by cholera toxin depends on the flotillin/reggie proteins but not Derlin-1 or -2
    David E Saslowsky
    Division of Gastroenterology, Children s Hospital, Boston, Massachusetts, USA
    J Clin Invest 120:4399-4409. 2010
    ..These results revise current models for CT intoxication and implicate protein scaffolding of lipid rafts in the endo-somal sorting of the toxin-GM1 complex...
  6. pmc Attenuated endocytosis and toxicity of a mutant cholera toxin with decreased ability to cluster ganglioside GM1 molecules
    Anne A Wolf
    GI Cell Biology, Enders 720, Children s Hospital Boston, 300 Longwood Ave, Boston, MA 02115, USA
    Infect Immun 76:1476-84. 2008
    ....
  7. ncbi request reprint Placental alkaline phosphatase is efficiently targeted to rafts in supported lipid bilayers
    David E Saslowsky
    Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
    J Biol Chem 277:26966-70. 2002
    ..We suggest that atomic force microscopy provides a powerful tool for the study of raft structure and properties...
  8. pmc Real-time analysis of the effects of cholesterol on lipid raft behavior using atomic force microscopy
    Jared C Lawrence
    Department of Pharmacology, University of Cambridge, United Kingdom
    Biophys J 84:1827-32. 2003
    ..Cholesterol colocalization with sphingomyelin-rich domains was confirmed using the sterol binding agent filipin...

Research Grants2

  1. Glycolipid Trafficking of Cholera Toxin by Lipid Rafts
    David Saslowsky; Fiscal Year: 2007
    ..This proposed research will discover novel host cell factors involved in the pathogenesis of cholera not yet ascribed to such a role, and may lead to new approaches for treatment and prevention ..