Joshua Sanes

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Agrin and synaptic laminin are required to maintain adult neuromuscular junctions
    Melanie A Samuel
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts, United States of America
    PLoS ONE 7:e46663. 2012
  2. pmc LL5beta: a regulator of postsynaptic differentiation identified in a screen for synaptically enriched transcripts at the neuromuscular junction
    Masashi Kishi
    Department of Anatomy and Neurobiology, Washington University Medical Center, St Louis, MO 63110, USA
    J Cell Biol 169:355-66. 2005
  3. pmc Protocadherins mediate dendritic self-avoidance in the mammalian nervous system
    Julie L Lefebvre
    Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 488:517-21. 2012
  4. pmc Shared resistance to aging and ALS in neuromuscular junctions of specific muscles
    Gregorio Valdez
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts, United States of America
    PLoS ONE 7:e34640. 2012
  5. pmc MEGF10 and MEGF11 mediate homotypic interactions required for mosaic spacing of retinal neurons
    Jeremy N Kay
    Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, 52 Oxford Street, Cambridge, Massachusetts 02138, USA
    Nature 483:465-9. 2012
  6. pmc Transgenic strategy for identifying synaptic connections in mice by fluorescence complementation (GRASP)
    Masahito Yamagata
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge MA, USA
    Front Mol Neurosci 5:18. 2012
  7. pmc Neurod6 expression defines new retinal amacrine cell subtypes and regulates their fate
    Jeremy N Kay
    Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA
    Nat Neurosci 14:965-72. 2011
  8. pmc A synaptic nidogen: developmental regulation and role of nidogen-2 at the neuromuscular junction
    Michael A Fox
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, MA 02138, USA
    Neural Dev 3:24. 2008
  9. pmc A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation
    Joanne S M Kim
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
    Neural Dev 3:23. 2008
  10. doi request reprint Many paths to synaptic specificity
    Joshua R Sanes
    Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Annu Rev Cell Dev Biol 25:161-95. 2009

Collaborators

Detail Information

Publications34

  1. pmc Agrin and synaptic laminin are required to maintain adult neuromuscular junctions
    Melanie A Samuel
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts, United States of America
    PLoS ONE 7:e46663. 2012
    ..Together, these results demonstrate that pre- and post-synaptic organizers actively function to maintain the structure and function of adult NMJs...
  2. pmc LL5beta: a regulator of postsynaptic differentiation identified in a screen for synaptically enriched transcripts at the neuromuscular junction
    Masashi Kishi
    Department of Anatomy and Neurobiology, Washington University Medical Center, St Louis, MO 63110, USA
    J Cell Biol 169:355-66. 2005
    ..Thus, a strategy designed to identify novel synaptic components led to identification of a protein required for assembly of the postsynaptic apparatus...
  3. pmc Protocadherins mediate dendritic self-avoidance in the mammalian nervous system
    Julie L Lefebvre
    Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 488:517-21. 2012
    ..Thus, although insect Dscam and vertebrate Pcdh proteins share no sequence homology, they seem to underlie similar strategies for endowing neurons with distinct molecular identities and patterning their arborizations...
  4. pmc Shared resistance to aging and ALS in neuromuscular junctions of specific muscles
    Gregorio Valdez
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts, United States of America
    PLoS ONE 7:e34640. 2012
    ..Together, these results reveal novel structural, regional and molecular parallels between aging and ALS...
  5. pmc MEGF10 and MEGF11 mediate homotypic interactions required for mosaic spacing of retinal neurons
    Jeremy N Kay
    Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, 52 Oxford Street, Cambridge, Massachusetts 02138, USA
    Nature 483:465-9. 2012
    ..Our results demonstrate that members of this gene family can also serve as subtype-specific ligands that pattern neuronal arrays...
  6. pmc Transgenic strategy for identifying synaptic connections in mice by fluorescence complementation (GRASP)
    Masahito Yamagata
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge MA, USA
    Front Mol Neurosci 5:18. 2012
    ..We also generated antisera that label reconstituted GFP but neither fragment in order to amplify the GRASP signal and thereby increase the sensitivity of the method...
  7. pmc Neurod6 expression defines new retinal amacrine cell subtypes and regulates their fate
    Jeremy N Kay
    Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA
    Nat Neurosci 14:965-72. 2011
    ..Our results support the view that cell fate decisions made in progenitors and their progeny act together to diversify ACs...
  8. pmc A synaptic nidogen: developmental regulation and role of nidogen-2 at the neuromuscular junction
    Michael A Fox
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, MA 02138, USA
    Neural Dev 3:24. 2008
    ..In each case, the synaptic isoform plays key roles in organizing the neuromuscular junction. Here, we analyze the fourth family, composed of nidogen-1 and -2...
  9. pmc A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation
    Joanne S M Kim
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
    Neural Dev 3:23. 2008
    ....
  10. doi request reprint Many paths to synaptic specificity
    Joshua R Sanes
    Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Annu Rev Cell Dev Biol 25:161-95. 2009
    ..Specification of any circuit is likely to involve integration of multiple mechanisms. Recent studies of vertebrate and invertebrate systems have led to the identification of molecules that mediate a few of these interactions...
  11. pmc Design principles of insect and vertebrate visual systems
    Joshua R Sanes
    Center for Brain Science, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Neuron 66:15-36. 2010
    ....
  12. ncbi request reprint Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system
    Jean Livet
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 450:56-62. 2007
    ..The ability of the Brainbow system to label uniquely many individual cells within a population may facilitate the analysis of neuronal circuitry on a large scale...
  13. pmc Ome sweet ome: what can the genome tell us about the connectome?
    Jeff W Lichtman
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, MA 02138, USA
    Curr Opin Neurobiol 18:346-53. 2008
    ..Remarkably similar objections were voiced when the Human Genome Project, now widely viewed as a success, was first proposed. We revisit that controversy to ask if it holds any lessons for proposals to map the connectome...
  14. pmc Laminar restriction of retinal ganglion cell dendrites and axons: subtype-specific developmental patterns revealed with transgenic markers
    In Jung Kim
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts 02138, USA
    J Neurosci 30:1452-62. 2010
    ..Taking account of these subtype-specific features will facilitate identification of the molecules and cells that regulate arbor formation...
  15. pmc A technicolour approach to the connectome
    Jeff W Lichtman
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts 02138, USA
    Nat Rev Neurosci 9:417-22. 2008
    ..Here we review recent advances in this area, along with some of the opportunities that these advances present and the obstacles that remain...
  16. ncbi request reprint Translational neuroscience during the Second World War
    Jeff W Lichtman
    Harvard University, USA
    J Exp Biol 209:3485-7. 2006
  17. ncbi request reprint Transgenic labeling of the corticospinal tract for monitoring axonal responses to spinal cord injury
    Florence M Bareyre
    Department of Molecular and Cellular Biology, Harvard University, Sherman Fairchild Building, Room 143, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Nat Med 11:1355-60. 2005
    ..Some of these new collaterals form additional direct synapses onto motoneurons. We propose that CST-YFP mice will be useful for evaluating strategies designed to maximize such remodeling and to promote regeneration...
  18. ncbi request reprint Versican in the developing brain: lamina-specific expression in interneuronal subsets and role in presynaptic maturation
    Masahito Yamagata
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Neurosci 25:8457-67. 2005
    ..We propose that versican provides a lamina-specific cue for presynaptic maturation and discuss the related but distinct effects of versican depletion and VVA blockade...
  19. doi request reprint Molecular identification of a retinal cell type that responds to upward motion
    In Jung Kim
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 452:478-82. 2008
    ..Thus, JAM-B identifies a unique population of RGCs in which structure corresponds remarkably to function...
  20. pmc Attenuation of age-related changes in mouse neuromuscular synapses by caloric restriction and exercise
    Gregorio Valdez
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 107:14863-8. 2010
    ..These results demonstrate a critical effect of aging on synaptic structure and provide evidence that interventions capable of extending health span and lifespan can partially reverse these age-related synaptic changes...
  21. pmc Retrograde influence of muscle fibers on their innervation revealed by a novel marker for slow motoneurons
    Joe V Chakkalakal
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA
    Development 137:3489-99. 2010
    ..This retrograde influence must be integrated with known anterograde influences in order to understand how motor units become homogeneous...
  22. ncbi request reprint Assembly of the postsynaptic membrane at the neuromuscular junction: paradigm lost
    Terrance T Kummer
    Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Curr Opin Neurobiol 16:74-82. 2006
    ..These recent studies challenge the widely-held paradigms, although not the results that led to them, and suggest a new model for neuromuscular synaptogenesis...
  23. pmc gamma-Protocadherins regulate neuronal survival but are dispensable for circuit formation in retina
    Julie L Lefebvre
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, MA 02138, USA
    Development 135:4141-51. 2008
    ....
  24. pmc Signal regulatory proteins (SIRPS) are secreted presynaptic organizing molecules
    Hisashi Umemori
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts 02138, USA
    J Biol Chem 283:34053-61. 2008
    ..SIRP family proteins may act together with other organizing molecules to pattern synapses...
  25. pmc Agrin promotes synaptic differentiation by counteracting an inhibitory effect of neurotransmitter
    Thomas Misgeld
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 102:11088-93. 2005
    ..Similar interactions between neurotransmitters and synaptic organizing molecules may operate at synapses in the central nervous system...
  26. doi request reprint Dscam and Sidekick proteins direct lamina-specific synaptic connections in vertebrate retina
    Masahito Yamagata
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 451:465-9. 2008
    ..Together, our results on Dscams and Sidekicks suggest the existence of an IgSF code for laminar specificity in retina and, by implication, in other parts of the central nervous system...
  27. ncbi request reprint Synaptotagmin I and II are present in distinct subsets of central synapses
    Michael A Fox
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts 02138, USA
    J Comp Neurol 503:280-96. 2007
    ..The cell-, temporal-, and species-specific expression of synaptotagmin isoforms suggests that each may have distinct functions in neurotransmitter release...
  28. pmc Dynamic remodeling of dendritic arbors in GABAergic interneurons of adult visual cortex
    Wei Chung Allen Lee
    The Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    PLoS Biol 4:e29. 2006
    ..These results are consistent with the idea that dendritic structural remodeling is a substrate for adult plasticity and they suggest that circuit rearrangement in the adult cortex is restricted by cell type-specific rules...
  29. ncbi request reprint Distinct target-derived signals organize formation, maturation, and maintenance of motor nerve terminals
    Michael A Fox
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Cell 129:179-93. 2007
    ..Thus, multiple target-derived signals permit discrete control of the formation, maturation, and maintenance of presynaptic specializations...
  30. pmc Synaptic localization and function of Sidekick recognition molecules require MAGI scaffolding proteins
    Masahito Yamagata
    Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Neurosci 30:3579-88. 2010
    ..These results demonstrate that localization and function of Sidekick-2 require its incorporation into a MAGI-containing synaptic scaffold...
  31. pmc Controlling the orientation and synaptic differentiation of myotubes with micropatterned substrates
    Jacinthe Gingras
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA
    Biophys J 97:2771-9. 2009
    ..Our results represent what we believe is a new approach for musculoskeletal tissue engineering, and our model sheds light on mechanisms of myotube alignment in vivo...
  32. pmc Birthdays of retinal amacrine cell subtypes are systematically related to their molecular identity and soma position
    P Emanuela Voinescu
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts 02138, USA
    J Comp Neurol 517:737-50. 2009
    ..Together, our results suggest that the birthdays of amacrine cells independently specify their destinations and subtype identities...
  33. pmc Laminins promote postsynaptic maturation by an autocrine mechanism at the neuromuscular junction
    Hiroshi Nishimune
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    J Cell Biol 182:1201-15. 2008
    ..Together with previous studies implicating laminins as organizers of presynaptic differentiation, these results show that laminins coordinate post- with presynaptic maturation...
  34. ncbi request reprint Non-invasive visualization of epidermal responses to injury using a fluorescent transgenic reporter
    Y Albert Pan
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA
    J Invest Dermatol 123:888-91. 2004
    ..These mice permit non-invasive time-lapse monitoring of responses to injury in vivo. They can be used to detect epidermal activation and to test agents that may provoke or attenuate epidermal responses...

Research Grants51

  1. EXTRACELLULAR MATRIX AND NEUROMUSCULAR DEVELOPMENT
    Joshua Sanes; Fiscal Year: 1992
    ..Through this work, we hope to gain some insight into how muscles use cell surface molecules to determine patterns of innervation...
  2. EXTRACELLULAR MATRIX AND NEUROMUSCULAR DEVELOPMENT
    Joshua Sanes; Fiscal Year: 2004
    ..Through this work, we hope to gain insight into molecules and mechanisms necessary for construction of synapses, which are the fundamental information-processing units of the nervous system. ..
  3. EXTRACELLULAR MATRIX AND NEUROMUSCULAR DEVELOPMENT
    Joshua Sanes; Fiscal Year: 2004
    ..Through this work, we hope to gain insight into molecules and mechanisms necessary for construction of synapses, which are the fundamental information-processing units of the nervous system. ..
  4. LINEAGE MIGRATION AND PHENOTYPIC CHOICE IN BRAIN
    Joshua Sanes; Fiscal Year: 1993
    ..These comparisons will provide insight into the variety of genealogical and migratory strategies that the nervous system uses to generate diversity...
  5. Synaptic choices in the retinotectal system
    Joshua Sanes; Fiscal Year: 2006
    ..Using these strategies, we hope eventually to elucidate mechanisms that promote lamina-specific synapse formation and, by extension, synaptic specificity generally ..
  6. EXTRACELLULAR MATRIX AND NEUROMUSCULAR DEVELOPMENT
    Joshua Sanes; Fiscal Year: 2002
    ..Through this work, we hope to gain insight into molecules and mechanisms necessary for construction of synapses, which are the fundamental information-processing units of the nervous system. ..
  7. EXTRACELLULAR MATRIX AND NEUROMUSCULAR DEVELOPMENT
    Joshua Sanes; Fiscal Year: 1999
    ..Through this work, we hope to gain insight into the retrograde signals that target cells use to determine patterns of innervation. ..
  8. EXTRACELLULAR MATRIX AND NEUROMUSCULAR DEVELOPMENT
    Joshua Sanes; Fiscal Year: 2000
    ..Through this work, we hope to gain insight into the retrograde signals that target cells use to determine patterns of innervation. ..
  9. EXTRACELLULAR MATRIX AND NEUROMUSCULAR DEVELOPMENT
    Joshua Sanes; Fiscal Year: 2001
    ..Through this work, we hope to gain insight into molecules and mechanisms necessary for construction of synapses, which are the fundamental information-processing units of the nervous system. ..
  10. PHENOTYPIC AND SYNAPTIC CHOICES IN THE OPTIC TECTUM
    Joshua Sanes; Fiscal Year: 2001
    ..The hope is that this strategy will eventually elucidate mechanisms that promote laminar-specific synapse formation in mammals. ..
  11. PHENOTYPIC AND SYNAPTIC CHOICES IN THE OPTIC TECTUM
    Joshua Sanes; Fiscal Year: 2002
    ..The hope is that this strategy will eventually elucidate mechanisms that promote laminar-specific synapse formation in mammals. ..
  12. Maturation and Maintenance of the Postsynaptic Apparatus
    Joshua Sanes; Fiscal Year: 2009
    ....
  13. LINEAGE MIGRATION AND PHENOTYPIC CHOICE IN BRAIN
    Joshua Sanes; Fiscal Year: 1992
    ..These comparisons will provide insight into the variety of genealogical and migratory strategies that the nervous system uses to generate diversity...
  14. EXTRACELLULAR MATRIX AND NEUROMUSCULAR DEVELOPMENT
    Joshua Sanes; Fiscal Year: 1999
    ..Through this work, we hope to gain insight into the retrograde signals that target cells use to determine patterns of innervation. ..
  15. Synaptic choices in the retinotectal system
    Joshua Sanes; Fiscal Year: 2009
    ..Using these strategies we hope eventually to elucidate mechanisms that promote lamina- specific synapse formation and, by extension, defects in specificity that may underlie behavioral disorders. ..
  16. Synaptic choices in the retinotectal system
    Joshua R Sanes; Fiscal Year: 2010
    ..Using these strategies we hope eventually to elucidate mechanisms that promote lamina- specific synapse formation and, by extension, defects in specificity that may underlie behavioral disorders. ..
  17. Synaptic choices in the retinotectal system
    Joshua Sanes; Fiscal Year: 2007
    ..Using these strategies, we hope eventually to elucidate mechanisms that promote lamina-specific synapse formation and, by extension, synaptic specificity generally ..
  18. Maturation and Maintenance of the Postsynaptic Apparatus
    Joshua R Sanes; Fiscal Year: 2010
    ....
  19. Synaptic choices in the retinotectal system
    Joshua Sanes; Fiscal Year: 2006
    ..Using these strategies, we hope eventually to elucidate mechanisms that promote lamina-specific synapse formation and, by extension, synaptic specificity generally ..
  20. PHENOTYPIC AND SYNAPTIC CHOICES IN THE OPTIC TECTUM
    Joshua Sanes; Fiscal Year: 1999
    ..The hope is that this strategy will eventually elucidate mechanisms that promote laminar-specific synapse formation in mammals. ..
  21. Synaptic choices in the retinotectal system
    Joshua Sanes; Fiscal Year: 2005
    ..Using these strategies, we hope eventually to elucidate mechanisms that promote lamina-specific synapse formation and, by extension, synaptic specificity generally ..
  22. LINEAGE MIGRATION AND PHENOTYPIC CHOICE IN BRAIN
    Joshua Sanes; Fiscal Year: 1991
    ..These comparisons will provide insight into the variety of genealogical and migratory strategies that the nervous system uses to generate diversity...
  23. EXTRACELLULAR MATRIX AND NEUROMUSCULAR DEVELOPMENT
    Joshua Sanes; Fiscal Year: 1993
    ..Through this work, we hope to gain some insight into how muscles use cell surface molecules to determine patterns of innervation...
  24. EXTRACELLULAR MATRIX AND NEUROMUSCULAR DEVELOPMENT
    Joshua Sanes; Fiscal Year: 1991
    ..Through this work, we hope to gain some insight into how muscles use cell surface molecules to determine patterns of innervation...
  25. EXTRACELLULAR MATRIX AND NEUROMUSCULAR DEVELOPMENT
    Joshua Sanes; Fiscal Year: 1990
    ..Through this work, we hope to gain some insight into how muscles use cell surface molecules to determine patterns of innervation...
  26. PHENOTYPIC AND SYNAPTIC CHOICES IN THE OPTIC TECTUM
    Joshua Sanes; Fiscal Year: 2000
    ..The hope is that this strategy will eventually elucidate mechanisms that promote laminar-specific synapse formation in mammals. ..
  27. PHENOTYPIC AND SYNAPTIC CHOICES IN THE OPTIC TECTUM
    Joshua Sanes; Fiscal Year: 2003
    ..The hope is that this strategy will eventually elucidate mechanisms that promote laminar-specific synapse formation in mammals. ..
  28. EXTRACELLULAR MATRIX AND NEUROMUSCULAR DEVELOPMENT
    Joshua Sanes; Fiscal Year: 2003
    ..Through this work, we hope to gain insight into molecules and mechanisms necessary for construction of synapses, which are the fundamental information-processing units of the nervous system. ..
  29. Synaptic choices in the retinotectal system
    Joshua Sanes; Fiscal Year: 2004
    ..Using these strategies, we hope eventually to elucidate mechanisms that promote lamina-specific synapse formation and, by extension, synaptic specificity generally ..
  30. Synaptic choices in the retinotectal system
    Joshua Sanes; Fiscal Year: 2009
    ..Using these strategies we hope eventually to elucidate mechanisms that promote lamina- specific synapse formation and, by extension, defects in specificity that may underlie behavioral disorders. ..