Genomes and Genes
Affiliation: Harvard University
- A chemical method for fast and sensitive detection of DNA synthesis in vivoAdrian Salic
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 105:2415-20. 2008..In contrast to BrdU, the method does not require sample fixation or DNA denaturation and permits good structural preservation. We demonstrate the use of the method in cultured cells and in the intestine and brain of whole animals...
- Vertebrate shugoshin links sister centromere cohesion and kinetochore microtubule stability in mitosisAdrian Salic
Department of Systems Biology, 240 Longwood Avenue, Boston, MA 02115, USA
Cell 118:567-78. 2004..Sgo is thus critical for mitotic progression and chromosome segregation and provides an unexpected link between sister centromere cohesion and microtubule interactions at kinetochores...
- Evidence for an upper limit to mitotic spindle lengthMartin Wühr
Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Curr Biol 18:1256-61. 2008..We conclude that early mitotic spindle length in Xenopus laevis is uncoupled from cell length, reaching an upper bound determined by mechanisms that are intrinsic to the spindle...
- The roles of APC and Axin derived from experimental and theoretical analysis of the Wnt pathwayEthan Lee
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA
PLoS Biol 1:E10. 2003..By applying control analysis to our mathematical model, we demonstrate the modular design, sensitivity, and robustness of the Wnt pathway and derive an explicit expression for tumor suppression and oncogenicity...
- The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assemblySrinath C Sampath
Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10021, USA
Cell 118:187-202. 2004..We propose that the chromosomal passenger complex regulates local MCAK activity to permit spindle formation via stabilization of chromatin-associated microtubules...
- Bub1 is essential for assembly of the functional inner centromereYekaterina Boyarchuk
Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Biol 176:919-28. 2007..We further provide evidence that Bub1 kinase activity is pivotal for recruitment of all of these components. Together, our findings demonstrate that Bub1 acts at multiple points to assure the correct kinetochore formation...
- Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2'-deoxyuridine antibodiesSuzanne B Buck
Invitrogen Corporation, Eugene, OR 97402, USA
Biotechniques 44:927-9. 2008..Elimination of this requirement results in the preservation of helical DNA structure and other cell surface epitopes, decreased assay time, and increased reproducibility...
- Lrp6 is required for convergent extension during Xenopus gastrulationEmilios Tahinci
Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
Development 134:4095-106. 2007..We propose a model in which Lrp6 plays a critical role in the switch from Wnt/PCP to Wnt/beta-catenin signaling...
- Identifying small molecule inhibitors of the ubiquitin-proteasome pathway in Xenopus egg extractsAdrian Salic
Methods Enzymol 399:567-85. 2005..Here we describe methods for identifying inhibitors of the proteolytic pathways that regulate cell cycle progression and Wnt signaling in Xenopus extracts...
- Anteroposterior patterning in hemichordates and the origins of the chordate nervous systemChristopher J Lowe
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
Cell 113:853-65. 2003..We propose that the deuterostome ancestor may have had a diffuse nervous system, which was later centralized during the evolution of the chordate lineage...
- A novel set of Wnt-Frizzled fusion proteins identifies receptor components that activate beta -catenin-dependent signalingSheri L Holmen
Laboratory of Cell Signaling and Carcinogenesis, Van Andel Research Institute, Grand Rapids, Michigan 49503, USA
J Biol Chem 277:34727-35. 2002..The data suggest that the distinction between the two Wnt classes lies not in intrinsic differences in the molecules but via the Frizzled molecules with which they interact...