Adrian Salic

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc A mechanism for vertebrate Hedgehog signaling: recruitment to cilia and dissociation of SuFu-Gli protein complexes
    Hanna Tukachinsky
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 191:415-28. 2010
  2. pmc A chemical method for fast and sensitive detection of DNA synthesis in vivo
    Adrian Salic
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:2415-20. 2008
  3. pmc Oxysterol binding to the extracellular domain of Smoothened in Hedgehog signaling
    Daniel Nedelcu
    1 Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA 2
    Nat Chem Biol 9:557-64. 2013
  4. pmc Dispatched and scube mediate the efficient secretion of the cholesterol-modified hedgehog ligand
    Hanna Tukachinsky
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell Rep 2:308-20. 2012
  5. pmc Processing and turnover of the Hedgehog protein in the endoplasmic reticulum
    Xin Chen
    Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 192:825-38. 2011
  6. pmc Evidence for an upper limit to mitotic spindle length
    Martin Wühr
    Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Curr Biol 18:1256-61. 2008
  7. pmc Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin
    Jing Liu
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 109:413-8. 2012
  8. ncbi request reprint Vertebrate shugoshin links sister centromere cohesion and kinetochore microtubule stability in mitosis
    Adrian Salic
    Department of Systems Biology, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 118:567-78. 2004
  9. pmc The roles of APC and Axin derived from experimental and theoretical analysis of the Wnt pathway
    Ethan Lee
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Biol 1:E10. 2003
  10. ncbi request reprint The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly
    Srinath C Sampath
    Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10021, USA
    Cell 118:187-202. 2004

Collaborators

  • Jing Liu
  • Timothy Mitchison
  • Sophie Dumont
  • Aaron C Groen
  • Xin Chen
  • Daniel J Needleman
  • Roland Krüger
  • Martin Wühr
  • Ryoma Ohi
  • M Dasso
  • Laura A Lee
  • Hanna Tukachinsky
  • Daniel Nedelcu
  • Ethan Lee
  • Suzanne B Buck
  • Yekaterina Boyarchuk
  • Emilios Tahinci
  • Srinath C Sampath
  • Christopher J Lowe
  • Marc W Kirschner
  • Sheri L Holmen
  • Cindy Jao
  • Yangqing Xu
  • Ryan P Kuzmickas
  • Cindy Y Jao
  • Lyle V Lopez
  • Jolene Bradford
  • Scott T Clarke
  • Kyle R Gee
  • Brian J Agnew
  • Alexei Arnaoutov
  • Curtis A Thorne
  • Kelly M Christian
  • Jeffrey L Franklin
  • Robert J Coffey
  • Oliver Leismann
  • Andrei Pozniakovski
  • Hironori Funabiki
  • Eric Lander
  • Mike Wu
  • John Gerhart
  • Louise Evans
  • Marc Kirschner
  • Reinhart Heinrich
  • Christian E Gruber
  • Nicole Stange-Thomann
  • J Gerhart
  • Cassandra R Zylstra
  • Bart O Williams

Detail Information

Publications16

  1. pmc A mechanism for vertebrate Hedgehog signaling: recruitment to cilia and dissociation of SuFu-Gli protein complexes
    Hanna Tukachinsky
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 191:415-28. 2010
    ..Our results support a simple mechanism in which Hh signals at vertebrate cilia cause dissociation of inactive SuFu-Gli complexes, a process inhibited by PKA...
  2. pmc A chemical method for fast and sensitive detection of DNA synthesis in vivo
    Adrian Salic
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:2415-20. 2008
    ..In contrast to BrdU, the method does not require sample fixation or DNA denaturation and permits good structural preservation. We demonstrate the use of the method in cultured cells and in the intestine and brain of whole animals...
  3. pmc Oxysterol binding to the extracellular domain of Smoothened in Hedgehog signaling
    Daniel Nedelcu
    1 Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA 2
    Nat Chem Biol 9:557-64. 2013
    ..Our results show that oxysterol binding to vertebrate Smo is required for normal Hh signaling and that targeting the oxysterol-binding site is an effective strategy to inhibit Smo. ..
  4. pmc Dispatched and scube mediate the efficient secretion of the cholesterol-modified hedgehog ligand
    Hanna Tukachinsky
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell Rep 2:308-20. 2012
    ..Thus, Disp and Scube cooperate to dramatically enhance the secretion and solubility of the cholesterol-modified Hh ligand...
  5. pmc Processing and turnover of the Hedgehog protein in the endoplasmic reticulum
    Xin Chen
    Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 192:825-38. 2011
    ..Thus, processing of the Hh precursor competes with its rapid degradation, explaining the impaired Hh signaling of processing-defective mutants, such as those causing human holoprosencephaly...
  6. pmc Evidence for an upper limit to mitotic spindle length
    Martin Wühr
    Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Curr Biol 18:1256-61. 2008
    ..We conclude that early mitotic spindle length in Xenopus laevis is uncoupled from cell length, reaching an upper bound determined by mechanisms that are intrinsic to the spindle...
  7. pmc Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin
    Jing Liu
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 109:413-8. 2012
    ..This strategy should have broad applicability for imaging protein synthesis and for identifying proteins synthesized under various physiological and pathological conditions in vivo...
  8. ncbi request reprint Vertebrate shugoshin links sister centromere cohesion and kinetochore microtubule stability in mitosis
    Adrian Salic
    Department of Systems Biology, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 118:567-78. 2004
    ..Sgo is thus critical for mitotic progression and chromosome segregation and provides an unexpected link between sister centromere cohesion and microtubule interactions at kinetochores...
  9. pmc The roles of APC and Axin derived from experimental and theoretical analysis of the Wnt pathway
    Ethan Lee
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Biol 1:E10. 2003
    ..By applying control analysis to our mathematical model, we demonstrate the modular design, sensitivity, and robustness of the Wnt pathway and derive an explicit expression for tumor suppression and oncogenicity...
  10. ncbi request reprint The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly
    Srinath C Sampath
    Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10021, USA
    Cell 118:187-202. 2004
    ..We propose that the chromosomal passenger complex regulates local MCAK activity to permit spindle formation via stabilization of chromatin-associated microtubules...
  11. pmc Bub1 is essential for assembly of the functional inner centromere
    Yekaterina Boyarchuk
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 176:919-28. 2007
    ..We further provide evidence that Bub1 kinase activity is pivotal for recruitment of all of these components. Together, our findings demonstrate that Bub1 acts at multiple points to assure the correct kinetochore formation...
  12. ncbi request reprint Anteroposterior patterning in hemichordates and the origins of the chordate nervous system
    Christopher J Lowe
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell 113:853-65. 2003
    ..We propose that the deuterostome ancestor may have had a diffuse nervous system, which was later centralized during the evolution of the chordate lineage...
  13. doi request reprint Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2'-deoxyuridine antibodies
    Suzanne B Buck
    Invitrogen Corporation, Eugene, OR 97402, USA
    Biotechniques 44:927-9. 2008
    ..Elimination of this requirement results in the preservation of helical DNA structure and other cell surface epitopes, decreased assay time, and increased reproducibility...
  14. ncbi request reprint Lrp6 is required for convergent extension during Xenopus gastrulation
    Emilios Tahinci
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Development 134:4095-106. 2007
    ..We propose a model in which Lrp6 plays a critical role in the switch from Wnt/PCP to Wnt/beta-catenin signaling...
  15. ncbi request reprint Identifying small molecule inhibitors of the ubiquitin-proteasome pathway in Xenopus egg extracts
    Adrian Salic
    Methods Enzymol 399:567-85. 2005
    ..Here we describe methods for identifying inhibitors of the proteolytic pathways that regulate cell cycle progression and Wnt signaling in Xenopus extracts...
  16. ncbi request reprint A novel set of Wnt-Frizzled fusion proteins identifies receptor components that activate beta -catenin-dependent signaling
    Sheri L Holmen
    Laboratory of Cell Signaling and Carcinogenesis, Van Andel Research Institute, Grand Rapids, Michigan 49503, USA
    J Biol Chem 277:34727-35. 2002
    ..The data suggest that the distinction between the two Wnt classes lies not in intrinsic differences in the molecules but via the Frizzled molecules with which they interact...