Research Topics
Genomes and GenesSpecies | R SacksteinSummaryAffiliation: Harvard University Country: USA Publications
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Publications
CD44-hyaluronic acid interactions mediate shear-resistant binding of lymphocytes to dermal endothelium in acute cutaneous GVHDMirjana Milinkovic
Harvard Institutes of Medicine, Rm 671, 77 Ave Louis Pasteur, Boston, MA 02115, USA
Blood 103:740-2. 2004..HA expressed at dermal endothelium in aGVHD is thus specialized to support lymphocyte adherence under flow conditions, and CD44-HA interactions may contribute to lymphocytotropism to skin in aGVHD...- A revision of Billingham's tenets: the central role of lymphocyte migration in acute graft-versus-host diseaseRobert Sackstein
Department of Dermatology, Brigham and Women s Hospital, Harvard Skin Disease Research Center, Harvard Medical School, Boston, MA 02115, USA
Biol Blood Marrow Transplant 12:2-8. 2006.... 
Shear flow-dependent integration of apical and subendothelial chemokines in T-cell transmigration: implications for locomotion and the multistep paradigmTaylor H Schreiber
Harvard Skin Disease Research Center and the Department of Dermatology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
Blood 109:1381-6. 2007....- Engineering cellular trafficking via glycosyltransferase-programmed stereosubstitutionRobert Sackstein
Department of Dermatology, Brigham and Women s Hospital, Boston, Massachusetts, USA
Ann N Y Acad Sci 1253:193-200. 2012..Without compromising cell viability or native phenotype, this exoglycosylation technology literally "sweetens" CD44, licensing E-selectin-dependent vascular delivery for all cell-based therapeutics... - Glycoengineering of HCELL, the human bone marrow homing receptor: sweetly programming cell migrationRobert Sackstein
Departments of Dermatology and of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
Ann Biomed Eng 40:766-76. 2012.... - The bone marrow is akin to skin: HCELL and the biology of hematopoietic stem cell homingRobert Sackstein
Departments of Dermatology and Medicine, Brigham and Women s Hospital, Harvard Skin Disease Research Center, Harvard Medical School, Boston, Massachusetts, USA
J Investig Dermatol Symp Proc 9:215-23. 2004.... - The lymphocyte homing receptors: gatekeepers of the multistep paradigmRobert Sackstein
Department of Dermatology and Medicine, Brigham and Women s Hospital, Harvard Skin Disease Research Center, Harvard Medical School, Boston, Massachusetts, USA
Curr Opin Hematol 12:444-50. 2005..This review focuses on the current and emerging perspectives of the biology of these 'homing receptors.'.. - The bone marrow is akin to skin: HCELL and the biology of hematopoietic stem cell homingRobert Sackstein
Department of Dermatology and Medicine, Brigham and Women s Hospital, Harvard Skin Disease Research Center, Harvard Medical School, Boston, Massachusetts, USA
J Invest Dermatol 122:1061-9. 2004.... 
Ex vivo glycan engineering of CD44 programs human multipotent mesenchymal stromal cell trafficking to boneRobert Sackstein
Department of Dermatology, Brigham and Women s Hospital and Harvard Skin Disease Research Center, Harvard Medical School, Boston, Massachusetts 02115, USA
Nat Med 14:181-7. 2008..These findings establish that the HCELL glycoform of CD44 confers tropism to bone and unveil a readily translatable roadmap for programming cellular trafficking by chemical engineering of glycans on a distinct membrane glycoprotein...- In vitro adherence of lymphocytes to dermal endothelium under shear stress: implications in pathobiology and steroid therapy of acute cutaneous GVHDRobert Sackstein
Harvard Skin Disease Research Center, Harvard Medical School, Department of Dermatology, Brigham and Women s Hospital, Boston, MA, USA
Blood 101:771-8. 2003.... - Glycosyltransferase-programmed stereosubstitution (GPS) to create HCELL: engineering a roadmap for cell migrationRobert Sackstein
Department of Dermatology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
Immunol Rev 230:51-74. 2009.... - A hematopoietic cell L-selectin ligand that is distinct from PSGL-1 and displays N-glycan-dependent binding activityR Sackstein
Bone Marrow Transplant Service, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA
Blood 96:2765-74. 2000..This novel ligand may help mediate L-selectin-dependent cell-cell adhesive interactions within the cytoarchitecture of the bone marrow microenvironment. (Blood. 2000;96:2765-2774).. - The biology of CD44 and HCELL in hematopoiesis: the 'step 2-bypass pathway' and other emerging perspectivesRobert Sackstein
Department of Dermatology, Brigham and Women s Hospital, Harvard Skin Disease Research Center, Harvard Medical School, Boston, Massachusetts 02115, USA
Curr Opin Hematol 18:239-48. 2011..This review focuses on recent advances in our understanding of the biology of CD44 and HCELL in hematopoiesis... - Western blot analysis of adhesive interactions under fluid shear conditions: the blot rolling assayRobert Sackstein
Department of Dermatology and Medicine, Brigham and Women s Hospital, 77 Avenue Louis Pasteur, Mailstop HIM, Room 671, Boston, MA, 02115, USA
Methods Mol Biol 536:343-54. 2009..This method thus allows for the identification, within a complex mixture and without prior isolation or purification, of both known and previously uncharacterized adhesion molecules operational under dynamic conditions... - differential L-selectin binding activities of human hematopoietic cell L-selectin ligands, HCELL and PSGL-1C J Dimitroff
Department of Dermatology, Harvard Skin Disease Research Center, Brigham and Women's Hospital, Harvard Medical School, 77 Ave. Louis Pasteur, Boston, MA 02115, USA
J Biol Chem 276:47623-31. 2001..Taken together, these data indicate that HCELL is a more avid L-selectin ligand than PSGL-1 and may be the preferential mediator of L-selectin-dependent adhesive interactions among human HCs in the bone marrow... - CD44 is a major E-selectin ligand on human hematopoietic progenitor cellsC J Dimitroff
Department of Dermatology, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
J Cell Biol 153:1277-86. 2001..These findings offer new insights into the structural biology and physiology of CD44, and into the molecular basis of E-selectin-dependent adhesive interactions that direct homing of human HPC to BM... - Mechanisms of thrombotic microangiopathy following xenogeneic hematopoietic progenitor cell transplantationI P Alwayn
The Transplantation Biology Research Center, Massachusetts General Hospital/Harvard Medical School, Boston, MA, USA
Transplantation 71:1601-9. 2001..Eptifibatide can fully abrogate platelet aggregation induced by porcine PBPC in vitro. Purification of the progenitor cells from porcine PBPC and/or treatment of baboons with eptifibatide may be beneficial... - A distinct glycoform of CD44 is an L-selectin ligand on human hematopoietic cellsC J Dimitroff
Department of Medicine and Dermatology, Brigham and Women s Hospital and Harvard Skin Disease Research Center, Harvard Medical School, Massachusetts General Hospital, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 97:13841-6. 2000..These data provide important insights on the structural biology of CD44 and reveal a role for this protein as an L-selectin ligand on human hematopoietic cells... - Glycosylation-dependent inhibition of cutaneous lymphocyte-associated antigen expression: implications in modulating lymphocyte migration to skinCharles J Dimitroff
Department of Dermatology and the Harvard Skin Disease Research Center at Brigham and Women s Hospital, Boston, MA, USA
Blood 101:602-10. 2003.... - Porcine hematopoietic cell xenotransplantation in nonhuman primates is complicated by thrombotic microangiopathyL Buhler
Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, USA
Bone Marrow Transplant 27:1227-36. 2001.... - Successful allogeneic stem cell transplantation with nonmyeloablative conditioning in patients with relapsed hematologic malignancy following autologous stem cell transplantationB R Dey
Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
Biol Blood Marrow Transplant 7:604-12. 2001..Further follow-up and evaluation of additional patients are required to conclusively establish the role of this strategy in the treatment of hematologic malignancies after an autologous transplantation... - HCELL is the major E- and L-selectin ligand expressed on LS174T colon carcinoma cellsMonica M Burdick
Department of Dermatology, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
J Biol Chem 281:13899-905. 2006.... - Direct real-time observation of E- and P-selectin-mediated rolling on cutaneous lymphocyte-associated antigen immobilized on Western blotsRobert C Fuhlbrigge
Department of Dermatology, Brigham and Women's Hospital, Boston, MA 02115, USA
J Immunol 168:5645-51. 2002.... - Directing stem cell trafficking via GPSRobert Sackstein
Department of Dermatology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
Methods Enzymol 479:93-105. 2010..Ex vivo glycan engineering of HCELL via GPS licenses trafficking of infused cells to endothelial beds that express E-selectin, thereby enabling efficient vascular delivery of stem/progenitor cells to sites where they are needed... - Mixed lymphohaemopoietic chimerism and graft-versus-lymphoma effects after non-myeloablative therapy and HLA-mismatched bone-marrow transplantationM Sykes
Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital and Harvard Medical School, Boston 02129, USA
Lancet 353:1755-9. 1999..We tested a new approach to find out whether lymphohaemopoietic graft-versus-host reactions could occur without excessive GVHD in mixed haemopoietic chimeras produced across HLA barriers with non-myeloablative conditioning... - Distribution of hematopoietic stem cells in the bone marrow according to regional hypoxiaKalindi Parmar
Department of Radiation Oncology, Dana Farber Cancer Institute, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 104:5431-6. 2007.... - G-CSF induces E-selectin ligand expression on human myeloid cellsNilesh M Dagia
Department of Dermatology, Brigham and Women s Hospital, 77 Avenue Louis Pasteur, Room 671, Harvard Medical School, Boston, Massachusetts 02115, USA
Nat Med 12:1185-90. 2006..These findings provide direct evidence for a role of G-CSF in the induction of E-selectin ligands on myeloid cells, thus providing mechanistic insight into the pathobiology of G-CSF complications... - Prevention of leukocyte migration to inflamed skin with a novel fluorosugar modifier of cutaneous lymphocyte-associated antigenCharles J Dimitroff
Harvard Skin Disease Research Center, Department of Dermatology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
J Clin Invest 112:1008-18. 2003..These data indicate that 4-F-GlcNAc prevents CHS by inhibiting selectin ligand activity and the capacity of effector T cells to enter antigen-challenged skin without affecting the afferent phase of CHS... - CD43 is a ligand for E-selectin on CLA+ human T cellsRobert C Fuhlbrigge
Department of Dermatology, Brigham and Women s Hospital, Harvard Institutes of Medicine, 77 Ave Louis Pasteur, Boston, MA 02115, USA
Blood 107:1421-6. 2006..The identification and characterization of CD43 as a T-cell E-selectin ligand distinct from PSGL-1 expands the role of CD43 in the regulation of T-cell trafficking and provides new targets for the modulation of immune functions in skin... - SDF-1/CXCR4 and VLA-4 interaction regulates homing in Waldenstrom macroglobulinemiaHai T Ngo
Medical Oncology, Dana Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA
Blood 112:150-8. 2008..Together, these studies demonstrate that the CXCR4/SDF-1 axis interacts with VLA-4 in regulating migration and adhesion of WM cells in the bone marrow microenvironment... - Impact of prophylactic donor leukocyte infusions on mixed chimerism, graft-versus-host disease, and antitumor response in patients with advanced hematologic malignancies treated with nonmyeloablative conditioning and allogeneic bone marrow transplantationBimalangshu R Dey
Bone Marrow Transplantation Program Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, USA
Biol Blood Marrow Transplant 9:320-9. 2003.... - The blot rolling assay: a method for identifying adhesion molecules mediating binding under shear conditionsRobert Sackstein
Harvard Skin Disease Research Center and Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
Methods Mol Biol 341:217-26. 2006..Thus, this method allows for the identification, within a complex mixture and without previous isolation or purification, of both known and novel adhesion molecules capable of binding under shear conditions... - Pig hematopoietic cell chimerism in baboons conditioned with a nonmyeloablative regimen and CD154 blockadeL Buhler
Transplantation Biology Research Center, Massachusetts General Hospital, MGH East, Building 149 9019, 13th Street, Boston, MA 02129, USA
Transplantation 73:12-22. 2002.... - A major fraction of human bone marrow lymphocytes are Th2-like CD1d-reactive T cells that can suppress mixed lymphocyte responsesM A Exley
Cancer Biology Program, Hematology Oncology, Beth Israel Deaconess Medical Center, Bone Marrow Transplant Program, Harvard Skin Disease Research Center, Harvard Medical School, Boston, MA 02115, USA
J Immunol 167:5531-4. 2001..Distinct CD1d-reactive T cell populations have the potential to suppress graft-vs-host disease and stimulate antitumor responses... - The BCR/ABL oncogene alters the chemotactic response to stromal-derived factor-1alphaR Salgia
Department of Medical Oncology, Division of Hematologic Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, USA
Blood 94:4233-46. 1999.... - Dynamic alterations in chemokine gradients induce transendothelial shuttling of human T cells under physiologic shear conditionsJack Y Lee
Harvard Skin Disease Research Center and Department of Dermatology, Brigham and Women s Hospital, Boston, Massachusetts, USA
J Leukoc Biol 86:1285-94. 2009..These findings broaden our understanding of chemokine biology and support a novel paradigm by which temporospatial modulations in subendothelial chemokine display drive cell migration from interstitial compartments into the bloodstream... - Porcine mononuclear cells adhere to human fibronectin independently of very late antigen-5: implications for donor-specific tolerance induction in xenotransplantationPierre R Theodore
Transplantation Biology Research Center, Surgical Service, Massachusetts General Hospital/Harvard Medical School, Boston, MA, USA
Xenotransplantation 9:277-89. 2002.... - CD15 expression in human myeloid cell differentiation is regulated by sialidase activitySamah Zeineb Gadhoum
Department of Dermatology, Brigham and Women s Hospital, 75 Francis Street, Boston, Massachusetts 02115, USA
Nat Chem Biol 4:751-7. 2008..Our results unveil a new paradigm by demonstrating a critical role for post-Golgi membrane glycosidase activity in the 'biosynthesis' of a key glycan determinant... - Anti-tumour response despite loss of donor chimaerism in patients treated with non-myeloablative conditioning and allogeneic stem cell transplantationBimalangshu R Dey
Department of Medicine Harvard Medical School Boston, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
Br J Haematol 128:351-9. 2005..Immunological mechanisms that correlated with rejection of the graft may have a role in anti-tumour responses via a cell or cytokine-mediated pathway... - Induction of kidney allograft tolerance after transient lymphohematopoietic chimerism in patients with multiple myeloma and end-stage renal diseaseLeo H Buhler
Department of Surgery, Massachusetts General Hospital, Boston, MA 02114, USA
Transplantation 74:1405-9. 2002.... - Fluctuating lymphocyte chimerism, tolerance and anti-tumor response in a patient with refractory lymphoma receiving nonmyeloablative conditioning and a haploidentical related allogeneic bone marrow transplantHan C Toh
Bone Marrow Transplant Program, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA
Cytokines Cell Mol Ther 7:43-7. 2002..Eventual lymphoma relapse temporally correlated with a generalised immunosuppressed state... - Peripheral blood progenitor cell product contains Th1-biased noninvariant CD1d-reactive natural killer T cells: implications for posttransplant survivalAngela Shaulov
Department of Hematology Oncology Beth Israel Deaconess Medical Center, Boston, Mass 02215, USA
Exp Hematol 36:464-72. 2008..Recent data suggests that NKT might contribute to clinical benefits of PBPC... - Early host CD8 T-cell recovery and sensitized anti-donor interleukin-2-producing and cytotoxic T-cell responses associated with marrow graft rejection following nonmyeloablative allogeneic bone marrow transplantationAnnette B Kraus
Bone Marrow Transplantation Section, Transplantation Biology Research Center, Department of Surgery, Massachusetts General Hospital/Harvard Medical School, Boston, Mass, USA
Exp Hematol 31:609-21. 2003..These data are the first to demonstrate sensitization of recipient anti-donor IL-2-producing cells in association with human marrow allograft rejection... - Nonmyeloablative bone marrow transplantation: Infectious complications in 65 recipients of HLA-identical and mismatched transplantsAndrew Daly
Allogeneic Bone Marrow Transplant Program, Princess Margaret Hospital University Health Network, University of Toronto, 610 University Avenue, Toronto, Ontario, Canada M5G 2M9
Biol Blood Marrow Transplant 9:373-82. 2003..We conclude that a quantitative T-cell deficiency in these extensively T-cell depleted patients may be a risk factor for infection, even in the absence of graft-versus-host disease... - Variant isoforms of CD44 are P- and L-selectin ligands on colon carcinoma cellsWilliam D Hanley
Department of The Johns Hopkins University, Baltimore, Maryland, USA
FASEB J 20:337-9. 2006..The novel finding that CD44v are selectin ligands offers a unifying perspective on the apparent enhanced metastatic potential associated with tumor cell CD44v overexpression and the critical role of selectins in metastasis... - CD44 on LS174T colon carcinoma cells possesses E-selectin ligand activityWilliam D Hanley
Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
Cancer Res 65:5812-7. 2005..Our finding that tumor-associated CD44 splice variant(s) express E-selectin ligand activity provides novel perspectives on the biology of CD44 in cancer metastasis... - L-selectin-mediated lymphocyte-cancer cell interactions under low fluid shear conditionsVicente A Resto
Department of Otolaryngology and the Sealy Center for Cancer Cell Biology, University of Texas Medical Branch, Galveston, Texas 77555, USA
J Biol Chem 283:15816-24. 2008..g. HCELL). These data define a novel class of L-selectin ligands and expand the scope of function for L-selectin within circulatory systems to now include a novel activity within shear stresses characteristic of lymphatic flow... - Primed marrow for autologous and allogeneic transplantation: a review comparing primed marrow to mobilized blood and steady-state marrowGerald J Elfenbein
Blood and Marrow Transplant Program, Roger Williams Medical Center, Providence, RI 02908, USA
Exp Hematol 32:327-39. 2004.... - Do G-CSF mobilized, peripheral blood-derived stem cells from healthy, HLA-identical donors really engraft more rapidly than do G-CSF primed, bone marrow-derived stem cells? No!Gerald J Elfenbein
Blood and Marrow Transplant Program, Roger Williams Medical Center, Providence, RI 02908, USA
Blood Cells Mol Dis 32:106-11. 2004..We propose the concept that the level of activation of the stem cells (induced by G-CSF) determines engraftment kinetics and not the anatomical site of derivation...
Research Grants
- Analysis of Homing Receptors on Human Adult Stem CellsRobert Sackstein; Fiscal Year: 2006....
- Structure & Biology of Hematopoietic E-/L-SelectinRobert Sackstein; Fiscal Year: 2006..This information will contribute greatly to our understanding of the adhesive systems that regulate steady-state and pathologic hematopoiesis, as well as hematopoietic recovery following stem cell transplantation. ..
- Molecular Analysis of CD44 on Colon Cancer CellsRobert Sackstein; Fiscal Year: 2007..It is anticipated that information gathered from these studies will form the basis for development of therapeutics targeting CD44 in colon cancer. ..
- Characterization of A Novel 65kDa E-selectin Ligand on G-CSF Mobilized LeukocytesRobert Sackstein; Fiscal Year: 2007..It is anticipated that the results obtained will lead to new approaches for preventing or alleviating G-CSF-induced vascular and inflammatory complications ..
- Optimizing Osteotropism of Human Mesenchymal Stem CellsRobert Sackstein; Fiscal Year: 2007....
- Molecular Analysis of CD44 on Colon Cancer CellsRobert Sackstein; Fiscal Year: 2009..It is anticipated that information gathered from these studies will form the basis for development of therapeutics targeting CD44 in colon cancer. ..
- Molecular Analysis of CD44 on Colon Cancer CellsRobert Sackstein; Fiscal Year: 2010..It is anticipated that information gathered from these studies will form the basis for development of therapeutics targeting CD44 in colon cancer. ..
- MOLECULAR ANALYSIS OF HEMATOPOIETIC L SELECTIN LIGANDRobert Sackstein; Fiscal Year: 2000....
- ADHESION MOLECULES MEDIATING SKIN TROPISM IN ACUTE GVHDRobert Sackstein; Fiscal Year: 2003....
- Optimizing Osteotropism of Human Mesenchymal Stem CellsRobert Sackstein; Fiscal Year: 2010....