ROBIN E REED

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi request reprint Mechanisms of fidelity in pre-mRNA splicing
    R Reed
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Curr Opin Cell Biol 12:340-5. 2000
  2. ncbi request reprint Coupling transcription, splicing and mRNA export
    Robin Reed
    Department of Cell Biology, 240 Longwood Avenue, Harvard Medical School, Boston, MA 02115, USA
    Curr Opin Cell Biol 15:326-31. 2003
  3. ncbi request reprint TREX, SR proteins and export of mRNA
    Robin Reed
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Curr Opin Cell Biol 17:269-73. 2005
  4. ncbi request reprint A new view of mRNA export: separating the wheat from the chaff
    R Reed
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Nat Cell Biol 3:E201-4. 2001
  5. ncbi request reprint Human mRNA export machinery recruited to the 5' end of mRNA
    Hong Cheng
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 127:1389-400. 2006
  6. pmc Functional coupling of RNAP II transcription to spliceosome assembly
    Rita Das
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 20:1100-9. 2006
  7. ncbi request reprint SR proteins function in coupling RNAP II transcription to pre-mRNA splicing
    Rita Das
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Mol Cell 26:867-81. 2007
  8. pmc Serine/arginine-rich protein-dependent suppression of exon skipping by exonic splicing enhancers
    El Cherif Ibrahim
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:5002-7. 2005
  9. ncbi request reprint An extensive network of coupling among gene expression machines
    Tom Maniatis
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 416:499-506. 2002
  10. ncbi request reprint A conserved mRNA export machinery coupled to pre-mRNA splicing
    Robin Reed
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 108:523-31. 2002

Collaborators

  • Steven Gygi
  • Susan A Slaugenhaupt
  • THOMAS P MANIATIS
  • Adrian R Krainer
  • C B Burge
  • KLEMENS HERTEL
  • Akiko Shimamura
  • Noam Shomron
  • Rita Das
  • El Cherif Ibrahim
  • Kobina Dufu
  • Matthew M Hims
  • Hong Cheng
  • Patricia Valencia
  • Karthik A Ganapathi
  • Chung Sheng Lee
  • Anusha Dias
  • Seiji Masuda
  • Chung Sheng Brian Lee
  • Ming Juan Luo
  • Zhaolan Zhou
  • Jan Seebacher
  • Stuart A Wilson
  • Michaela J Livingstone
  • Anusha P Dias
  • Sandra Gill
  • Conxi Lazaro
  • Maire Leyne
  • Lijuan Liu
  • Jiong Yu
  • Karyn M Austin
  • James Mull
  • Melanie P Gygi
  • Ranjit S Shetty
  • James F Gusella
  • Maggie M Malsch
  • Zuo Zhang
  • Ben Romney
  • Jeanne L Hsu
  • Megan Feldt
  • Mark Elenko
  • Nijsje Dorman
  • Ed Hurt
  • Thomas D Schaal

Detail Information

Publications19

  1. ncbi request reprint Mechanisms of fidelity in pre-mRNA splicing
    R Reed
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Curr Opin Cell Biol 12:340-5. 2000
    ....
  2. ncbi request reprint Coupling transcription, splicing and mRNA export
    Robin Reed
    Department of Cell Biology, 240 Longwood Avenue, Harvard Medical School, Boston, MA 02115, USA
    Curr Opin Cell Biol 15:326-31. 2003
    ..Finally, a newly identified link between the nuclear exosome and the machineries required for transcription, 3'-end formation and mRNA export suggests that proper mRNP formation is co-transcriptionally monitored...
  3. ncbi request reprint TREX, SR proteins and export of mRNA
    Robin Reed
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Curr Opin Cell Biol 17:269-73. 2005
    ..There is also an interesting possibility that an SR protein-TREX complex interaction is a conserved part of the mRNA export machinery...
  4. ncbi request reprint A new view of mRNA export: separating the wheat from the chaff
    R Reed
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Nat Cell Biol 3:E201-4. 2001
    ..A machinery that is conserved between yeast and higher eukaryotes functions to export the mRNA...
  5. ncbi request reprint Human mRNA export machinery recruited to the 5' end of mRNA
    Hong Cheng
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 127:1389-400. 2006
    ..As a consequence, the mRNA would be exported in a 5' to 3' direction through the nuclear pore, as observed in early electron micrographs of giant Balbiani ring mRNPs...
  6. pmc Functional coupling of RNAP II transcription to spliceosome assembly
    Rita Das
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 20:1100-9. 2006
    ..We conclude that RNAP II mediates the functional coupling of transcription to splicing by directing the nascent pre-mRNA into spliceosome assembly, thereby bypassing interaction of the pre-mRNA with the inhibitory hnRNP proteins...
  7. ncbi request reprint SR proteins function in coupling RNAP II transcription to pre-mRNA splicing
    Rita Das
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Mol Cell 26:867-81. 2007
    ..Thus, these factors readily out-compete inhibitory hnRNP proteins, resulting in efficient spliceosome assembly on nascent RNAP II transcripts...
  8. pmc Serine/arginine-rich protein-dependent suppression of exon skipping by exonic splicing enhancers
    El Cherif Ibrahim
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:5002-7. 2005
    ..We conclude that exonic enhancers can act as barriers to prevent exon skipping and thereby may play a key role in ensuring the correct 5' to 3' linear order of exons in spliced mRNA...
  9. ncbi request reprint An extensive network of coupling among gene expression machines
    Tom Maniatis
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 416:499-506. 2002
    ..The extensive coupling is consistent with a model in which the machines are tethered to each other to form 'gene expression factories' that maximize the efficiency and specificity of each step in gene expression...
  10. ncbi request reprint A conserved mRNA export machinery coupled to pre-mRNA splicing
    Robin Reed
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 108:523-31. 2002
    ..Export of mRNAs is coupled to upstream steps in gene expression, such as pre-mRNA splicing, and to downstream events, including nonsense-mediated decay...
  11. pmc The human Shwachman-Diamond syndrome protein, SBDS, associates with ribosomal RNA
    Karthik A Ganapathi
    Department of Pediatric Hematology, Children s Hospital Boston, Boston, MA, USA
    Blood 110:1458-65. 2007
    ..Our studies support the addition of SDS to the growing list of human bone marrow failure syndromes involving the ribosome...
  12. doi request reprint Purification of functional RNA-protein complexes using MS2-MBP
    Zhaolan Zhou
    Children s Hospital and Harvard Medical School, Boston, Massachusetts, USA
    Curr Protoc Mol Biol . 2003
    ..The purified spliceosome preparation is used to determine the protein components of the spliceosome by mass spectrometry and to examine the structure of the spliceosome by electron microscopy...
  13. doi request reprint Identification of RNA binding proteins by UV cross-linking
    Ming Juan Luo
    Harvard Medical School, Boston, Massachusetts, USA
    Curr Protoc Mol Biol . 2003
    ..Identification the direct interactions between proteins and RNA has greatly advanced our knowledge about the function of RNA-protein machines. UV crosslinking is a straightforward and powerful tool in this endeavor...
  14. pmc Splicing promotes rapid and efficient mRNA export in mammalian cells
    Patricia Valencia
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:3386-91. 2008
    ..We conclude that splicing promotes mRNA export in mammalian cells and that the functional coupling between splicing and mRNA export is a conserved and general feature of gene expression in higher eukaryotes...
  15. doi request reprint Agarose gel separation/isolation of RNA-protein complexes
    Chung Sheng Brian Lee
    Harvard Medical School, Boston, Massachusetts, USA
    Curr Protoc Mol Biol . 2003
    ..Moreover, the use of low-melting point agarose for the fractionation readily allows for the isolation of the RNA species in each complex detected on the native gel...
  16. pmc ATP is required for interactions between UAP56 and two conserved mRNA export proteins, Aly and CIP29, to assemble the TREX complex
    Kobina Dufu
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 24:2043-53. 2010
    ..We conclude that the interaction of two conserved export proteins, CIP29 and Aly, with UAP56 is strictly regulated by ATP during assembly of the TREX complex...
  17. pmc Recruitment of the human TREX complex to mRNA during splicing
    Seiji Masuda
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 19:1512-7. 2005
    ....
  18. ncbi request reprint Therapeutic potential and mechanism of kinetin as a treatment for the human splicing disease familial dysautonomia
    Matthew M Hims
    Center for Human Genetic Research, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    J Mol Med (Berl) 85:149-61. 2007
    ..Lastly, we highlight the potential of kinetin for the treatment of other human splicing disorders by showing correction of a splicing defect in neurofibromatosis...
  19. ncbi request reprint Weak definition of IKBKAP exon 20 leads to aberrant splicing in familial dysautonomia
    El Cherif Ibrahim
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Hum Mutat 28:41-53. 2007
    ....

Research Grants27

  1. Functional Coupling of Steps in Gene Expression
    Robin Reed; Fiscal Year: 2009
    ..A newly established mammalian system for assaying export of intronless mRNAs will be combined with biochemical studies to define the cis-acting sequences involved in mRNA export and the factors that function in this process. ..
  2. Functional Coupling of Steps in Gene Expression
    ROBIN E REED; Fiscal Year: 2010
    ..A newly established mammalian system for assaying export of intronless mRNAs will be combined with biochemical studies to define the cis-acting sequences involved in mRNA export and the factors that function in this process. ..
  3. MECHANISMS OF PREMRNA SPLICING IN HIGHER EUKARYOTES
    Robin Reed; Fiscal Year: 2002
    ..We plan to use both systematic analysis of mutant pre-mRNAs and characterization of proteins (hPrp16, hPrp17 and hSlu7) specifically involved in catalytic step II in order to investigate the step II mechanism. ..
  4. THE LINK BETWEEN SPLICING & EXPORT OF MESSENGER RNA
    Robin Reed; Fiscal Year: 2003
    ..Together, these studies should provide important new insights into the mechanism of mRNA export and the link between splicing and export. ..
  5. Coupling Transcription, Splicing and mRNA Export
    Robin Reed; Fiscal Year: 2006
    ..abstract_text> ..
  6. Functional Coupling of Steps in Genes Expression
    Robin Reed; Fiscal Year: 2007
    ..abstract_text> ..
  7. MECHANISMS OF PRE-MRNA SPLICING IN HIGHER EUKARYOTES
    Robin Reed; Fiscal Year: 1993
    ..These studies analysis of the spliceosome will be carried out using electron microscopy. These studies should provide unique information on the spatial arrangement of pre-mRNA and splicing components within the spliceosome...
  8. Functional Coupling of Steps in Gene Expression
    Robin Reed; Fiscal Year: 2009
    ..The major goal of this project is to achieve a detailed understanding of the mechanisms and factors involved in the functional coupling between transcription, splicing and mRNA export. ..