Anjana Rao

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc A common factor regulates both Th1- and Th2-specific cytokine gene expression
    J W Rooney
    Department of Genetics, Harvard Medical School, Boston, MA 02115
    EMBO J 13:625-33. 1994
  2. pmc Recombinant NFAT1 (NFATp) is regulated by calcineurin in T cells and mediates transcription of several cytokine genes
    C Luo
    Division of Cellular and Molecular Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 16:3955-66. 1996
  3. pmc Calcium signaling in lymphocytes
    Masatsugu Oh-hora
    Department of Pathology, Harvard Medical School, Immune Disease Institute, Boston, MA 02115, USA
    Curr Opin Immunol 20:250-8. 2008
  4. ncbi Transcription factors of the NFAT family: regulation and function
    A Rao
    Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Immunol 15:707-47. 1997
  5. ncbi Modulation of chromatin structure regulates cytokine gene expression during T cell differentiation
    S Agarwal
    Department of Pathology, Harvard Medical School, Center for Blood Research, Boston, Massachusetts 02115, USA
    Immunity 9:765-75. 1998
  6. ncbi New functions for DNA binding domains
    A Rao
    Center for Blood Research and the Department of Pathology, Harvard Medical School, Warren Alpert Building, 200 Longwood Avenue, Boston, MA 02115, USA
    Sci STKE 2001:pe1. 2001
  7. ncbi Role of NFAT proteins in IL13 gene transcription in mast cells
    Silvia Monticelli
    Department of Pathology, Harvard Medical School, and CBR Institute for Biomedical Research, Boston, MA 02115, USA
    J Biol Chem 279:36210-8. 2004
  8. ncbi Deletion of a conserved Il4 silencer impairs T helper type 1-mediated immunity
    K Mark Ansel
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Immunol 5:1251-9. 2004
  9. pmc Hair loss and defective T- and B-cell function in mice lacking ORAI1
    Yousang Gwack
    Department of Pathology, Harvard Medical School and the Immune Disease Institute, Rm 152, Warren Alpert Bldg, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Mol Cell Biol 28:5209-22. 2008
  10. pmc Runx3 and T-box proteins cooperate to establish the transcriptional program of effector CTLs
    Fernando Cruz-Guilloty
    Harvard Medical School and the Immune Disease Institute, Boston, MA 02115, USA
    J Exp Med 206:51-9. 2009

Detail Information

Publications117 found, 100 shown here

  1. pmc A common factor regulates both Th1- and Th2-specific cytokine gene expression
    J W Rooney
    Department of Genetics, Harvard Medical School, Boston, MA 02115
    EMBO J 13:625-33. 1994
    ..We propose that NF-ATp is a common regulatory factor for both Th1 and Th2 cytokine genes, and that the involvement of PKC-dependent factors, such as AP-1, may help determine Th1-/Th2-specific patterns of gene expression...
  2. pmc Recombinant NFAT1 (NFATp) is regulated by calcineurin in T cells and mediates transcription of several cytokine genes
    C Luo
    Division of Cellular and Molecular Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 16:3955-66. 1996
    ....
  3. pmc Calcium signaling in lymphocytes
    Masatsugu Oh-hora
    Department of Pathology, Harvard Medical School, Immune Disease Institute, Boston, MA 02115, USA
    Curr Opin Immunol 20:250-8. 2008
    ..This review focuses on the signaling pathways upstream and downstream of Ca(2+) influx (the STIM/ORAI and calcineurin/NFAT pathways, respectively)...
  4. ncbi Transcription factors of the NFAT family: regulation and function
    A Rao
    Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Immunol 15:707-47. 1997
    ....
  5. ncbi Modulation of chromatin structure regulates cytokine gene expression during T cell differentiation
    S Agarwal
    Department of Pathology, Harvard Medical School, Center for Blood Research, Boston, Massachusetts 02115, USA
    Immunity 9:765-75. 1998
    ..We propose that chromatin remodeling of cytokine gene loci is functionally associated with productive T cell differentiation and may explain the coordinate regulation of Th2 cytokine genes...
  6. ncbi New functions for DNA binding domains
    A Rao
    Center for Blood Research and the Department of Pathology, Harvard Medical School, Warren Alpert Building, 200 Longwood Avenue, Boston, MA 02115, USA
    Sci STKE 2001:pe1. 2001
    ..This change in structure may influence the binding of activators or repressors of transcription, thus allowing a single transcription factor to have two different activities...
  7. ncbi Role of NFAT proteins in IL13 gene transcription in mast cells
    Silvia Monticelli
    Department of Pathology, Harvard Medical School, and CBR Institute for Biomedical Research, Boston, MA 02115, USA
    J Biol Chem 279:36210-8. 2004
    ..We suggest that mast cells lack a co-activator protein that stabilizes the binding of NFAT2 to the IL13 promoter by interacting either with NFAT2 itself or with a DNA-bound complex of NFAT2 and GATA proteins...
  8. ncbi Deletion of a conserved Il4 silencer impairs T helper type 1-mediated immunity
    K Mark Ansel
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Immunol 5:1251-9. 2004
    ....
  9. pmc Hair loss and defective T- and B-cell function in mice lacking ORAI1
    Yousang Gwack
    Department of Pathology, Harvard Medical School and the Immune Disease Institute, Rm 152, Warren Alpert Bldg, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Mol Cell Biol 28:5209-22. 2008
    ....
  10. pmc Runx3 and T-box proteins cooperate to establish the transcriptional program of effector CTLs
    Fernando Cruz-Guilloty
    Harvard Medical School and the Immune Disease Institute, Boston, MA 02115, USA
    J Exp Med 206:51-9. 2009
    ..Our data point to the existence of an elaborate transcriptional network in which Runx3 initially induces and then cooperates with T-box transcription factors to regulate gene transcription in differentiating CTLs...
  11. pmc Hyperactivation of nuclear factor of activated T cells 1 (NFAT1) in T cells attenuates severity of murine autoimmune encephalomyelitis
    Srimoyee Ghosh
    Department of Pathology, Harvard Medical School and Immune Disease Institute, Children s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 107:15169-74. 2010
    ....
  12. pmc Requirement for balanced Ca/NFAT signaling in hematopoietic and embryonic development
    Martin R Muller
    Department of Pathology and Immune Disease Institute, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 106:7034-9. 2009
    ....
  13. ncbi Regulation of the germinal center response by microRNA-155
    To Ha Thai
    CBR Institute for Biomedical Research, Harvard Medical School, Boston, MA 02115, USA
    Science 316:604-8. 2007
    ..miR-155 exerts this control, at least in part, by regulating cytokine production. These results also suggest that individual microRNAs can exert critical control over mammalian differentiation processes in vivo...
  14. pmc Interleukin-2 and inflammation induce distinct transcriptional programs that promote the differentiation of effector cytolytic T cells
    Matthew E Pipkin
    Harvard Medical School, Boston, MA 02115, USA Immune Disease Institute, Boston, MA 02115, USA
    Immunity 32:79-90. 2010
    ..Thus, inflammation influences both effector and memory CTL differentiation, whereas persistent IL-2 stimulation promotes effector at the expense of memory CTL development...
  15. pmc Molecular analysis of a locus control region in the T helper 2 cytokine gene cluster: a target for STAT6 but not GATA3
    Dong U Lee
    Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:16010-5. 2004
    ..We suggest that the RAD50 LCR has a complex and dual role in Th1 and Th2 differentiation, communicating early T cell antigen receptor and cytokine signals to the IL-4/IL-13 locus in both differentiating cell types...
  16. pmc A 10-aa-long sequence in SLP-76 upstream of the Gads binding site is essential for T cell development and function
    Lalit Kumar
    Division of Immunology, Children s Hospital, CBR Institute for Biomedical Research, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:19063-8. 2005
    ..These results indicate that the Lck binding region of SLP-76 is essential for T cell antigen receptor signaling and normal T cell development and function...
  17. ncbi A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function
    Stefan Feske
    The CBR Institute for Biomedical Research, and the Department of Pediatrics, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 441:179-85. 2006
    ..We propose that Orai1 is an essential component or regulator of the CRAC channel complex...
  18. ncbi Biochemical and functional characterization of Orai proteins
    Yousang Gwack
    Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    J Biol Chem 282:16232-43. 2007
    ..Together, these data establish Orai1 as a predominant mediator of store-operated calcium entry, proliferation, and cytokine production in T cells...
  19. ncbi A 3' enhancer in the IL-4 gene regulates cytokine production by Th2 cells and mast cells
    Deborah C Solymar
    The Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Immunity 17:41-50. 2002
    ..These results identify an essential enhancer which regulates IL-4 gene expression in two important cell lineages in vivo...
  20. ncbi Involvement of NFAT1 in B cell self-tolerance
    Robert A Barrington
    CBR Institute for Biomedical Research, Harvard University, Boston, MA 02115, USA
    J Immunol 177:1510-5. 2006
    ..Taken together, these studies provide direct evidence that the transcription factor NFAT1 is involved in B cell anergy...
  21. ncbi A molecular dissection of lymphocyte unresponsiveness induced by sustained calcium signalling
    Vigo Heissmeyer
    Department of Pathology, Harvard Medical School, CBR Institute for Biomedical Research, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Novartis Found Symp 267:165-74; discussion 174-9. 2005
    ..Thus Ca(2+)-calcineurin-NFAT signalling links gene transcription to a multi-step programme that leads to impaired signal transduction in anergic T cells...
  22. pmc Halofuginone inhibits TH17 cell differentiation by activating the amino acid starvation response
    Mark S Sundrud
    Department of Pathology, Harvard Medical School and Immune Disease Institute, Boston, MA 02115, USA
    Science 324:1334-8. 2009
    ..These results indicate that the AAR pathway is a potent and selective regulator of inflammatory T cell differentiation in vivo...
  23. ncbi A distal enhancer in the interferon-gamma (IFN-gamma) locus revealed by genome sequence comparison
    Dong U Lee
    Center for Blood Research and the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:4802-10. 2004
    ..Together, these findings identify a highly conserved distal enhancer in the IFN-gamma cytokine locus and validate our approach as a successful method to detect cis-regulatory elements...
  24. ncbi Signalling to transcription: store-operated Ca2+ entry and NFAT activation in lymphocytes
    Yousang Gwack
    Department of Pathology, Harvard Medical School, The CBR Institute for Biomedical Research, 200 Longwood Avenue, Boston, MA 02115, USA
    Cell Calcium 42:145-56. 2007
    ..These approaches, together with subsequent mutational and electrophysiological analyses, converged to identify human Orai1 as a pore subunit of the CRAC channel and as the gene product mutated in the SCID patients...
  25. ncbi Calcineurin imposes T cell unresponsiveness through targeted proteolysis of signaling proteins
    Vigo Heissmeyer
    Center for Blood Research and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Nat Immunol 5:255-65. 2004
    ..Our results define a complex molecular program that links gene transcription induced by calcium and calcineurin to a paradoxical impairment of signal transduction in anergic T cells...
  26. pmc Dephosphorylation of the nuclear factor of activated T cells (NFAT) transcription factor is regulated by an RNA-protein scaffold complex
    Sonia Sharma
    Department of Pathology, Harvard Medical School, Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital Boston, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 108:11381-6. 2011
    ....
  27. ncbi Chromatin-based regulation of cytokine transcription in Th2 cells and mast cells
    Silvia Monticelli
    Department of Pathology, Harvard Medical School, and CBR Institute for Biomedical Research, Boston, MA 02115, USA
    Int Immunol 17:1513-24. 2005
    ....
  28. pmc Dual functions for the endoplasmic reticulum calcium sensors STIM1 and STIM2 in T cell activation and tolerance
    Masatsugu Oh-hora
    Harvard Medical School and Immune Disease Institute, Boston, Massachusetts 02115, USA
    Nat Immunol 9:432-43. 2008
    ..We conclude that both STIM1 and STIM2 promote store-operated Ca2+ entry into T cells and fibroblasts and that STIM proteins are required for the development and function of regulatory T cells...
  29. pmc Tet1 and Tet2 regulate 5-hydroxymethylcytosine production and cell lineage specification in mouse embryonic stem cells
    Kian Peng Koh
    Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital Boston, Boston, MA 02115, USA
    Cell Stem Cell 8:200-13. 2011
    ..Thus, 5hmC is an epigenetic modification associated with the pluripotent state, and Tet1 functions to regulate the lineage differentiation potential of ESCs...
  30. ncbi Regulation of Th2 differentiation and Il4 locus accessibility
    K Mark Ansel
    Harvard Medical School, CBR Institute for Biomedical Research, Boston, Massachusetts 02115, USA
    Annu Rev Immunol 24:607-56. 2006
    ....
  31. ncbi A genome-wide Drosophila RNAi screen identifies DYRK-family kinases as regulators of NFAT
    Yousang Gwack
    The CBR Institute for Biomedical Research and the Departments of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 441:646-50. 2006
    ..Thus, genetic screening in Drosophila can be successfully applied to cross evolutionary boundaries and identify new regulators of a transcription factor that is expressed only in vertebrates...
  32. ncbi T(H) cell differentiation is accompanied by dynamic changes in histone acetylation of cytokine genes
    Orly Avni
    The Center for Blood Research and the Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Nat Immunol 3:643-51. 2002
    ....
  33. pmc A conserved docking motif for CK1 binding controls the nuclear localization of NFAT1
    Heidi Okamura
    CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 24:4184-95. 2004
    ..The CK1 docking motif is present in proteins of the Wnt, Hedgehog, and circadian-rhythm pathways, which also integrate the activities of CK1 and GSK3...
  34. ncbi Transcription factors T-bet and Runx3 cooperate to activate Ifng and silence Il4 in T helper type 1 cells
    Ivana M Djuretic
    Harvard Medical School and the CBR Institute for Biomedical Research, Boston, Massachusetts 02115, USA
    Nat Immunol 8:145-53. 2007
    ..Our data indicate that cytokine gene expression in T(H)1 cells may be controlled by a feed-forward regulatory circuit in which T-bet induces Runx3 and then 'partners' with Runx3 to direct lineage-specific gene activation and silencing...
  35. ncbi Regulation of interferon-gamma gene expression by nuclear factor of activated T cells
    A Kiani
    Department of Pathology, Harvard Medical School, and the Center for Blood Research, Boston, MA 02115, USA
    Blood 98:1480-8. 2001
    ..These results suggest that IFN-gamma production by T cells is regulated by NFAT1, most likely at the level of gene transcription...
  36. ncbi Structural delineation of the calcineurin-NFAT interaction and its parallels to PP1 targeting interactions
    Huiming Li
    Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    J Mol Biol 342:1659-74. 2004
    ....
  37. pmc Aberrant T cell differentiation in the absence of Dicer
    Stefan A Muljo
    The CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    J Exp Med 202:261-9. 2005
    ..Independent of their proliferation defect, Dicer-deficient helper T cells preferentially expressed interferon-gamma, the hallmark effector cytokine of the Th1 lineage...
  38. ncbi Structure of calcineurin in complex with PVIVIT peptide: portrait of a low-affinity signalling interaction
    Huiming Li
    The CBR Institute, for Biomedical Research, 200 Longwood Avenue, Boston, MA 02115, USA
    J Mol Biol 369:1296-306. 2007
    ....
  39. pmc STIM1 gates the store-operated calcium channel ORAI1 in vitro
    Yubin Zhou
    Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital, Boston, Massachusetts, USA
    Nat Struct Mol Biol 17:112-6. 2010
    ....
  40. pmc Immunological applications of genomics
    Silvia Monticelli
    Harvard Medical School and the CBR Institute for Biomedical Research, Boston, MA 02115, USA
    Genome Biol 7:321. 2006
    ..A report of the Cold Spring Harbor Laboratory meeting 'Gene Expression and Signaling in the Immune System', Cold Spring Harbor, New York, USA, 26-30 April 2006...
  41. ncbi NFAT1 and NFAT2 are positive regulators of IL-4 gene transcription
    Silvia Monticelli
    Department of Pathology, Harvard Medical School, and the Center for Blood Research, Alpert Building Rm 152, 200 Longwood Avenue, Boston, MA 02115, USA
    Eur J Immunol 32:2971-8. 2002
    ....
  42. pmc Pore architecture of the ORAI1 store-operated calcium channel
    Yubin Zhou
    Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital Boston, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 107:4896-901. 2010
    ..The cross-linking data further identify a relatively rigid segment of TM1 adjacent to E106 that is likely to contribute to the selectivity filter...
  43. pmc Transcriptional complexes formed by NFAT dimers regulate the induction of T cell tolerance
    Noemi Soto-Nieves
    Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Exp Med 206:867-76. 2009
    ..These data also establish a basis for the design of immunomodulatory strategies that specifically target each type of complex...
  44. doi Orphans against autoimmunity
    Hozefa Bandukwala
    Department of Pathology, Harvard Medical School and Immune Disease Institute, 200 Longwood Avenue, Boston, MA 02115, USA
    Immunity 29:167-8. 2008
    ..In this issue of Immunity, Hermann-Kleiter et al. (2008) identify a nuclear orphan receptor, NR2F6, as a negative regulator of the T helper 17 cell subset and report that NR2F6-deficient mice develop late-onset autoimmune disease...
  45. ncbi Foxp1 is an essential transcriptional regulator of B cell development
    Hui Hu
    Department of Pathology, Harvard Medical School, CBR Institute for Biomedical Research, Boston, Massachusetts 02115, USA
    Nat Immunol 7:819-26. 2006
    ..Our results identify Foxp1 as an essential participant in the transcriptional regulatory network of B lymphopoiesis...
  46. ncbi Cell-type-restricted binding of the transcription factor NFAT to a distal IL-4 enhancer in vivo
    S Agarwal
    Department of Pathology, Harvard Medical School and The Center for Blood Research, Boston, Massachusetts 02115, USA
    Immunity 12:643-52. 2000
    ..This restricted access enables antigen-dependent and subset-specific transcription of cytokine genes...
  47. pmc Normal peripheral T-cell function in c-Fos-deficient mice
    J Jain
    Division of Tumor Virology, Dana Farber Cancer Institute, Boston, Massachusetts 02115
    Mol Cell Biol 14:1566-74. 1994
    ..Our results suggest that other Fos family members may be capable of substituting functionally for c-Fos during T-cell development and cytokine gene transcription in activated T cells...
  48. ncbi Transcriptional mechanisms underlying lymphocyte tolerance
    Fernando Macian
    Center for Blood Research, Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Cell 109:719-31. 2002
    ..Thus, in the absence of AP-1, NFAT imposes a genetic program of lymphocyte anergy that counters the program of productive activation mediated by the cooperative NFAT:AP-1 complex...
  49. ncbi Bioinformatics for the 'bench biologist': how to find regulatory regions in genomic DNA
    Julie Nardone
    Department of Pathology, Harvard Medical School and the CBR Institute for Biomedical Research, Boston, Massachusetts 02115, USA
    Nat Immunol 5:768-74. 2004
    ....
  50. ncbi Th2 lineage commitment and efficient IL-4 production involves extended demethylation of the IL-4 gene
    Dong U Lee
    Department of Pathology, Harvard Medical School and The Center for Blood Research, Boston, MA 02115, USA
    Immunity 16:649-60. 2002
    ..5' demethylation is not required for chromatin remodeling or primary transcription of the IL-4 gene but is strongly associated with efficient, high-level induction of IL-4 transcripts by differentiated Th2 cells...
  51. ncbi The histone H3K4 demethylase SMCX links REST target genes to X-linked mental retardation
    Mamta Tahiliani
    Division of Endocrinology, Diabetes, and Hypertension, Department of Medicine and BCMP, Brigham and Women s Hospital and Harvard Medical School, 221 Longwood Avenue Boston, Massachusetts 02115, USA
    Nature 447:601-5. 2007
    ..We propose that loss of SMCX activity impairs REST-mediated neuronal gene regulation, thereby contributing to SMCX-associated X-linked mental retardation...
  52. pmc Impaired hydroxylation of 5-methylcytosine in myeloid cancers with mutant TET2
    Myunggon Ko
    Department of Pathology, Harvard Medical School, Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    Nature 468:839-43. 2010
    ..Measurement of 5hmC levels in myeloid malignancies may prove valuable as a diagnostic and prognostic tool, to tailor therapies and assess responses to anticancer drugs...
  53. pmc Genome-wide mapping of 5-hydroxymethylcytosine in embryonic stem cells
    William A Pastor
    Harvard Medical School, Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    Nature 473:394-7. 2011
    ..Our results indicate that 5hmC has a probable role in transcriptional regulation, and suggest a model in which 5hmC contributes to the 'poised' chromatin signature found at developmentally-regulated genes in ES cells...
  54. ncbi TID1, a mammalian homologue of the drosophila tumor suppressor lethal(2) tumorous imaginal discs, regulates activation-induced cell death in Th2 cells
    Josh Syken
    Department of Pathology, Harvard Medical School, 200 Longwood Avenue, D2 544A, Boston, MA 02115 5701, USA
    Oncogene 22:4636-41. 2003
    ..Hence, the accumulation of Tid-1S in Th2 cells following activation represents a novel mechanism that may contribute to the induction of apoptosis resistance during the activation of Th2 cells...
  55. pmc Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1
    Mamta Tahiliani
    Department of Pathology, Harvard Medical School and Immune Disease Institute, 200 Longwood Avenue, Boston, MA 02115, USA
    Science 324:930-5. 2009
    ..hmC is present in the genome of mouse embryonic stem cells, and hmC levels decrease upon RNA interference-mediated depletion of TET1. Thus, TET proteins have potential roles in epigenetic regulation through modification of 5mC to hmC...
  56. ncbi Regulation of gene expression in mast cells: micro-rNA expression and chromatin structural analysis of cytokine genes
    Silvia Monticelli
    Department of Pathology, Harvard Medical School, and CBR Institute for Biomedical Research, Boston, MA 02115, USA
    Novartis Found Symp 271:179-87; discussion 187-90, 198-9. 2005
    ..We are studying the biological functions of selected miRNAs...
  57. pmc Regulation of CD45 alternative splicing by heterogeneous ribonucleoprotein, hnRNPLL
    Shalini Oberdoerffer
    Department of Pathology, Immune Disease Institute, Harvard Medical School, Boston, MA 02115, USA
    Science 321:686-91. 2008
    ..Induction of hnRNPLL during hematopoietic cell activation and differentiation may allow cells to rapidly shift their transcriptomes to favor proliferation and inhibit cell death...
  58. ncbi Transcriptional regulation by calcium, calcineurin, and NFAT
    Patrick G Hogan
    The Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 17:2205-32. 2003
  59. ncbi An epigenetic view of helper T cell differentiation
    K Mark Ansel
    Center for Blood Research, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Immunol 4:616-23. 2003
    ..Recent studies have begun to elucidate the molecular mechanisms that establish and maintain polarized cytokine gene expression, and thus the cellular identity of differentiated helper T cells...
  60. ncbi Down-regulation of IL-4 gene transcription and control of Th2 cell differentiation by a mechanism involving NFAT1
    A Kiani
    The Center for Blood Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Immunity 7:849-60. 1997
    ..Consistent with this observation, NFAT1-/- mice are more susceptible to infection with Leishmania major. This report provides evidence that NFAT proteins regulate not only the initiation but also the termination of gene transcription...
  61. ncbi Gene regulation mediated by calcium signals in T lymphocytes
    S Feske
    Center for Blood Research and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Nat Immunol 2:316-24. 2001
    ..We demonstrate an elaborate network of signaling pathways downstream of the T cell receptor, explaining the complexity of changes in gene expression during T cell activation...
  62. ncbi Selective inhibition of NFAT activation by a peptide spanning the calcineurin targeting site of NFAT
    J Aramburu
    Center for Blood Research, Harvard Medical School, Boston Massachusetts 02115, USA
    Mol Cell 1:627-37. 1998
    ..Thus, disruption of the enzyme-substrate docking interaction that directs calcineurin to NFAT can effectively block NFAT-dependent functions...
  63. ncbi The transcription factor NFAT3 mediates neuronal survival
    Alessandra B Benedito
    Center for Blood Research, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 280:2818-25. 2005
    ..Taken together, these results reveal an essential function for NFAT3-mediated transcription in neuronal survival that may play important roles in the developing and mature brain...
  64. pmc Domain requirements and sequence specificity of DNA binding for the forkhead transcription factor FOXP3
    Kian Peng Koh
    Department of Pathology, Harvard Medical School and Immune Disease Institute, Boston, Massachusetts, United States of America
    PLoS ONE 4:e8109. 2009
    ..These results establish a practical framework for understanding the molecular basis by which FOXP3 regulates gene transcription and programs Treg suppressive function...
  65. ncbi New twists of T cell fate: control of T cell activation and tolerance by TGF-beta and NFAT
    Mark S Sundrud
    Department of Pathology, Harvard Medical School, and The CBR Institute for Biomedical Research Inc, Boston, MA 02115, USA
    Curr Opin Immunol 19:287-93. 2007
    ..Recent findings have begun to shed light on the mechanisms by which TGF-beta and NFAT integrate multiple signaling inputs to determine the direction of naïve T-cell differentiation...
  66. ncbi Transcriptional basis of lymphocyte tolerance
    Madhuri Borde
    The CBR Institute for Biomedical Research and the Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Immunol Rev 210:105-19. 2006
    ....
  67. pmc A CD8 T cell-intrinsic role for the calcineurin-NFAT pathway for tolerance induction in vivo
    Thomas Fehr
    Transplantation Biology Research Center, Massachusetts General Hospital Harvard Medical School, Boston, MA, USA
    Blood 115:1280-7. 2010
    ..Thus, our study reveals a CD8 T cell-intrinsic NFAT1 requirement for CD8 tolerance in vivo...
  68. ncbi Ca2+/calcineurin signalling in cells of the immune system
    Stefan Feske
    Department of Pathology, Harvard Medical School, Boston and The CBR Institute for Biomedical Research, 200 Longwood Ave, Boston, MA 02115, USA
    Biochem Biophys Res Commun 311:1117-32. 2003
    ..This review focuses on recent studies elucidating the role of Ca(2+)/calcineurin signalling of the immune system...
  69. pmc NFAT5 binds to the TNF promoter distinctly from NFATp, c, 3 and 4, and activates TNF transcription during hypertonic stress alone
    Jonathan H Esensten
    CBR Institute for Biomedical Research, Department of Pathology, Harvard Medical School 800 Huntington Avenue, Boston, MA 02115, USA
    Nucleic Acids Res 33:3845-54. 2005
    ....
  70. pmc Reciprocal modulatory interaction between human immunodeficiency virus type 1 Tat and transcription factor NFAT1
    F Macian
    Department of Pathology, Harvard Medical School, and Center for Blood Research, Boston, Massachusetts 02115, USA
    Mol Cell Biol 19:3645-53. 1999
    ..We discuss the potentially opposing roles of NFAT1 and another family member, NFAT2, in regulating gene transcription of HIV-1 and endogenous cytokine genes...
  71. pmc The behaviour of 5-hydroxymethylcytosine in bisulfite sequencing
    Yun Huang
    Department of Pathology, Harvard Medical School and Immune Disease Institute, Boston, Massachusetts, United States of America
    PLoS ONE 5:e8888. 2010
    ..Since 5-hmC reacts with bisulfite to yield cytosine 5-methylenesulfonate (CMS), we asked how DNA containing 5-hmC behaves in bisulfite sequencing...
  72. ncbi Partners in transcription: NFAT and AP-1
    F Macian
    Department of Pathology, Harvard Medical School and The Center for Blood Research, 200 Longwood Avenue, Boston, Massachusetts, MA 02115, USA
    Oncogene 20:2476-89. 2001
    ..Balanced activation of NFAT and AP-1 is known to be required for productive immune responses, but the role of NFAT:AP-1 interactions in other cell types and biological processes remains to be understood...
  73. ncbi Regulation of allergic inflammation and eosinophil recruitment in mice lacking the transcription factor NFAT1: role of interleukin-4 (IL-4) and IL-5
    J P Viola
    Center for Blood Research and the Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Blood 91:2223-30. 1998
    ..Thus, the presence of NFAT1 might inhibit the allergic response, perhaps by interfering with the development of Th2 immune responses, and the lack or dysfunction of NFAT1 could potentially underlie certain cases of atopic disease...
  74. pmc Nuclear factor of activated T cells (NFAT)-dependent transactivation regulated by the coactivators p300/CREB-binding protein (CBP)
    C Garcia-Rodriguez
    Department of Pathology, Harvard Medical School, and the Center for Blood Research, Boston, Massachusetts 02115, USA
    J Exp Med 187:2031-6. 1998
    ..Recruitment of the coactivators p300/CBP by the transactivation domains of NFAT proteins is likely to play a critical role in NFAT-dependent gene expression during the immune response...
  75. ncbi Bridging the NFAT and NF-kappaB families: NFAT5 dimerization regulates cytokine gene transcription in response to osmotic stress
    C Lopez-Rodriguez
    The Center for Blood Research and, Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Immunity 15:47-58. 2001
    ..We suggest that NFAT5 participates in specific aspects of host defense by upregulating TNF family genes and other target genes in T cells...
  76. ncbi Concerted dephosphorylation of the transcription factor NFAT1 induces a conformational switch that regulates transcriptional activity
    H Okamura
    The Center for Blood Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 6:539-50. 2000
    ..This conformational switch paradigm may explain modification-induced functional changes in other heavily phosphorylated proteins...
  77. pmc MicroRNA profiling of the murine hematopoietic system
    Silvia Monticelli
    Department of Pathology, Harvard Medical School, and CBR Institute for Biomedical Research, Boston, MA 02115, USA
    Genome Biol 6:R71. 2005
    ..It is becoming clear that miRNAs play an important role in the regulation of gene expression during development. However, in mammals, expression data are principally based on whole tissue analysis and are still very incomplete...
  78. pmc Gene regulation and signal transduction in the immune system
    Tiffany Horng
    Department of Pathology, Harvard Medical School, Immune Disease Institute, Boston, Massachusetts 02115, USA
    Genome Biol 9:315. 2008
    ..A report of the meeting 'Gene Expression and Signaling in the Immune System', Cold Spring Harbor, USA, 22-26 April 2008...
  79. pmc Loss of NFAT5 results in renal atrophy and lack of tonicity-responsive gene expression
    Cristina Lopez-Rodriguez
    Department of Pathology, Harvard Medical School and Center for Blood Research, Institute for Biomedical Research, 200 Longwood Avenue, Boston MA 02115, USA
    Proc Natl Acad Sci U S A 101:2392-7. 2004
    ..Our findings demonstrate a central role for NFAT5 as a tonicity-responsive transcription factor required for kidney homeostasis and function...
  80. doi Introduction to COI volume on lymphocyte activation 2008
    Anjana Rao
    Department of Pathology, Harvard Medical School, Boston, MA 02115, USA Immune Disease Institute, Room 152, Warren Alpert Building, 200 Longwood Avenue, Boston, MA 02115, USA
    Curr Opin Immunol 20:247-9. 2008
  81. pmc Efficiency of RNA interference in the mouse hematopoietic system varies between cell types and developmental stages
    Philipp Oberdoerffer
    The CBR Institute for Biomedical Research, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell Biol 25:3896-905. 2005
    ..The extent of gene inactivation varies between different cell types and is least efficient in mature lymphocytes. Our data suggest that RNAi is affected by factors beyond small interfering RNA-mRNA stoichiometry...
  82. ncbi Dissecting ICRAC, a store-operated calcium current
    Patrick G Hogan
    The CBR Institute for Biomedical Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Trends Biochem Sci 32:235-45. 2007
    ....
  83. pmc Molecular basis of calcium signaling in lymphocytes: STIM and ORAI
    Patrick G Hogan
    Department of Pathology, Harvard Medical School, Immune Disease Institute, Children s Hospital Boston, Massachusetts 02115, USA
    Annu Rev Immunol 28:491-533. 2010
    ..In this review, we discuss selected aspects of Ca(2+) signaling in cells of the immune system, focusing on the roles of STIM and ORAI proteins in store-operated Ca(2+) entry...
  84. ncbi Integration of TCR and IL-4 signals through STAT6 and the regulation of IL-4 gene expression
    C B Schmidt-Weber
    Immunology Research Division, Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Mol Immunol 37:767-74. 2000
    ..Calcineurin-mediated serine dephosphorylation of STAT6 and STAT6 serine phosphorylation may counter-regulate transcriptional activity of STAT6...
  85. ncbi Long-range transcriptional regulation of cytokine gene expression
    S Agarwal
    Center for Blood Research, Boston, MA 02115, USA
    Curr Opin Immunol 10:345-52. 1998
    ..These studies have begun to elucidate the basis for cell-specificity and high-level expression of cytokine genes...
  86. pmc NFAT5, a constitutively nuclear NFAT protein that does not cooperate with Fos and Jun
    C Lopez-Rodriguez
    Department of Pathology, Harvard Medical School, The Center for Blood Research, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 96:7214-9. 1999
    ....
  87. ncbi Impaired NFAT regulation and its role in a severe combined immunodeficiency
    S Feske
    The Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA
    Immunobiology 202:134-50. 2000
    ....
  88. ncbi T cell differentiation: a mechanistic view
    O Avni
    Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Curr Opin Immunol 12:654-9. 2000
    ..These changes require the coordinate actions of antigen- and cytokine-induced transcription factors, chromatin remodeling complexes, histone-modifying enzymes and subset-specific transcription factors...
  89. ncbi NF-AT5: the NF-AT family of transcription factors expands in a new direction
    C Lopez-Rodriguez
    Center for Blood Research and the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cold Spring Harb Symp Quant Biol 64:517-26. 1999
  90. ncbi Transcriptional regulation in lymphocytes
    H Okamura
    Department of Pathology, Harvard Medical School and The Center for Blood Research, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Curr Opin Cell Biol 13:239-43. 2001
    ..The emerging theme is one of cell-type-specific regulation, affecting not only the functional activation of transcription factors but also their access to appropriate regions of DNA...
  91. ncbi Wedelolactone suppresses LPS-induced caspase-11 expression by directly inhibiting the IKK complex
    M Kobori
    Department of Cell Biology, Harvard Medical School, Boston, MA, USA
    Cell Death Differ 11:123-30. 2004
    ..We demonstrate that wedelolactone is an inhibitor of IKK, a kinase critical for activation of NF-kappaB by mediating phosphorylation and degradation of IkappaBalpha...
  92. ncbi Structure of the DNA-binding domains from NFAT, Fos and Jun bound specifically to DNA
    L Chen
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 392:42-8. 1998
    ..The tight association of the three proteins on DNA creates a continuous groove for the recognition of 15 base pairs...
  93. doi Regulation of gene expression in peripheral T cells by Runx transcription factors
    Ivana M Djuretic
    Department of Pathology, Harvard Medical School and Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital Boston, Boston, Massachusetts, USA
    Adv Immunol 104:1-23. 2009
    ..Here, we review gene regulation by Runx proteins in T lymphocytes, with a focus on their recently emerging roles in the development and function of peripheral CD4+ and CD8+ T lineages...
  94. pmc A severe defect in CRAC Ca2+ channel activation and altered K+ channel gating in T cells from immunodeficient patients
    Stefan Feske
    CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    J Exp Med 202:651-62. 2005
    ..They also offer evidence for a functional link between CRAC and Kv1.3 channels, and establish a model system for molecular genetic studies of the CRAC channel...
  95. ncbi The role of NFAT transcription factors in integrin-mediated carcinoma invasion
    Sebastien Jauliac
    Department of Pathology, Harvard Medical School and Beth Israel Deaconess Medical Center, 200 Longwood Avenue, Boston MA 02115, USA
    Nat Cell Biol 4:540-4. 2002
    ..These observations show that NFATs are targets of alpha(6)beta(4) integrin signalling and are involved in promoting carcinoma invasion, highlighting a novel function for this family of transcription factors in human cancer...
  96. ncbi Normal function of the transcription factor NFAT1 in wasted mice. Chromosome localization of NFAT1 gene
    C Luo
    Division of Cellular and Molecular Biology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Gene 180:29-36. 1996
    ..Therefore, the wasted phenotype is not due to a defect in the expression or early regulation of the NFAT1 protein...
  97. ncbi Expression of the transcription factor NFATp in a neuronal cell line and in the murine nervous system
    A M Ho
    Department of Neurobiology, Harvard Medical School, Boston, Massachusetts
    J Biol Chem 269:28181-6. 1994
    ..The presence of NFATp in the nervous system suggests that it has a role in the transcription of specific neuronal genes in response to increases in cytosolic calcium...
  98. ncbi A similar DNA-binding motif in NFAT family proteins and the Rel homology region
    J Jain
    Dana Farber Cancer Institute, Department of Pathology, Boston, Massachusetts 02115
    J Biol Chem 270:4138-45. 1995
    ..The results suggest that NFATp and Rel family proteins bind to DNA using similar structural motifs...
  99. ncbi An asymmetric NFAT1 dimer on a pseudo-palindromic kappa B-like DNA site
    Lei Jin
    Department of Biological Chemistry and Molecular Pharmacology and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Struct Biol 10:807-11. 2003
    ..The structure we have determined may correspond to a functional NFAT binding mode at palindromic sites of genes induced during the anergic response to weak TCR signaling...
  100. pmc ORAI1 deficiency and lack of store-operated Ca2+ entry cause immunodeficiency, myopathy, and ectodermal dysplasia
    Christie Ann McCarl
    Department of Pathology, New York University, Langone Medical Center, New York, NY 10016, USA
    J Allergy Clin Immunol 124:1311-1318.e7. 2009
    ..T-cell activation requires Ca2+ influx through Ca2+-release activated Ca2+ (CRAC) channels encoded by the gene ORAI1...
  101. pmc Selective inhibition of calcineurin-NFAT signaling by blocking protein-protein interaction with small organic molecules
    Michael H A Roehrl
    Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:7554-9. 2004
    ....

Research Grants4

  1. CAPACITATIVE CALCIUM ENTRY DEFECT IN IMMUNE DEFICIENCY
    Anjana Rao; Fiscal Year: 2002
    ..Deletion mapping using spectral karyotyping and microsatellite markers will be used to further narrow down the chromosomal region involved in the defect and candidate genes in this region will be evaluated. ..
  2. Manipulation of T cell tolerance with small organic mol*
    Anjana Rao; Fiscal Year: 2005
    ..The hope is that the 'information obtained from this pilot R21 proposal will eventually be extended to whole animal models of transplant and tumour rejection, allergy and autoimmune disease. ..
  3. Role of micro-RNAs in T cells and other immune/haematopoietic cells
    Anjana Rao; Fiscal Year: 2007
    ..Together these studies should greatly increase our understanding of the role of miRNAs in T cells and other cells of the immune and hematopoietic systems. ..