Genomes and Genes


Hidde L Ploegh


Affiliation: Harvard University
Country: USA


  1. Fang T, Van Elssen C, Duarte J, Guzman J, Chahal J, Ling J, et al. Targeted antigen delivery by an anti-class II MHC VHH elicits focused αMUC1(Tn) immunity. Chem Sci. 2017;8:5591-5597 pubmed publisher
    ..A single boost 12 months after primary immunization triggered a recall response of the same quality, suggesting that long-term αMUC1(Tn) memory had been achieved. ..
  2. Pishesha N, Ingram J, Ploegh H. Sortase A: A Model for Transpeptidation and Its Biological Applications. Annu Rev Cell Dev Biol. 2018;34:163-188 pubmed publisher
    ..We focus primarily on the transpeptidase sortase A, which has become popular over the past few years for its ability to perform a remarkably wide variety of protein modifications, both in vitro and in living cells. ..
  3. Ingram J, Blomberg O, Rashidian M, Ali L, Garforth S, Fedorov E, et al. Anti-CTLA-4 therapy requires an Fc domain for efficacy. Proc Natl Acad Sci U S A. 2018;115:3912-3917 pubmed publisher
    ..Coadministration of the monovalent H11 VHH blocks the efficacy of a full-sized therapeutic antibody. We were thus able to demonstrate that CTLA-4-binding antibodies require an Fc domain for antitumor effect. ..
  4. Truttmann M, Pincus D, Ploegh H. Chaperone AMPylation modulates aggregation and toxicity of neurodegenerative disease-associated polypeptides. Proc Natl Acad Sci U S A. 2018;115:E5008-E5017 pubmed publisher
    ..We thus define a link between HSP70 AMPylation and the dynamics of protein aggregation in neurodegenerative disease models. Our results are consistent with a cytoprotective, rather than a cytotoxic, role for such protein aggregates. ..
  5. Ingram J, Blomberg O, Sockolosky J, Ali L, Schmidt F, Pishesha N, et al. Localized CD47 blockade enhances immunotherapy for murine melanoma. Proc Natl Acad Sci U S A. 2017;114:10184-10189 pubmed publisher
    ..Thus, strategies to localize CD47 blockade to tumors may be particularly valuable for immune therapy. ..
  6. Xie Y, Dougan M, Jailkhani N, Ingram J, Fang T, KUMMER L, et al. Nanobody-based CAR T cells that target the tumor microenvironment inhibit the growth of solid tumors in immunocompetent mice. Proc Natl Acad Sci U S A. 2019;116:7624-7631 pubmed publisher
    ..Our results demonstrate the flexibility of VHH-based CAR T cells and the potential of CAR T cells to target the tumor microenvironment and treat solid tumors. ..
  7. Kaur M, Kumar D, Butty V, Singh S, Esteban A, Fink G, et al. Galectin-3 Regulates γ-Herpesvirus Specific CD8 T Cell Immunity. iScience. 2018;9:101-119 pubmed publisher
    ..Targeting intracellular galectin-3 in CD8+ T cells may therefore serve to enhance response to efficiently control infections. ..
  8. Fang T, Lu X, Berger D, Gmeiner C, Cho J, Schalek R, et al. Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure. Nat Methods. 2018;15:1029-1032 pubmed publisher
    ..This technique allowed the large-scale 3D reconstruction of a volume of mouse hippocampal CA3 tissue at nanometer resolution. ..
  9. Ingram J, Schmidt F, Ploegh H. Exploiting Nanobodies' Singular Traits. Annu Rev Immunol. 2018;36:695-715 pubmed publisher
    ..Here we review such applications, with particular emphasis on those areas where conventional antibodies would face a more challenging environment. ..

More Information


  1. Chahal J, Fang T, Woodham A, Khan O, Ling J, Anderson D, et al. An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model. Sci Rep. 2017;7:252 pubmed publisher
    ..These results demonstrate that this approach can be used to evaluate new candidate antigens and identify immune correlates without the use of live virus. ..
  2. Hanke L, Schmidt F, Knockenhauer K, Morin B, Whelan S, Schwartz T, et al. Vesicular stomatitis virus N protein-specific single-domain antibody fragments inhibit replication. EMBO Rep. 2017;18:1027-1037 pubmed publisher
    ..Collectively, these binding sites uncover different features on the N protein surface that may be suitable for antiviral intervention. ..
  3. Truttmann M, Ploegh H. rAMPing Up Stress Signaling: Protein AMPylation in Metazoans. Trends Cell Biol. 2017;27:608-620 pubmed publisher
    ..We review endogenous as well as parasitic protein AMPylation in metazoans and summarize current views of how fic-domain-containing AMPylases contribute to cellular proteostasis. ..
  4. Rashidian M, Ingram J, Dougan M, Dongre A, Whang K, LeGall C, et al. Predicting the response to CTLA-4 blockade by longitudinal noninvasive monitoring of CD8 T cells. J Exp Med. 2017;214:2243-2255 pubmed publisher
    ..It may thus be possible to use immuno-PET and monitor antitumor immune responses as a prognostic tool to predict patient responses to checkpoint therapies. ..
  5. Ingram J, Dougan M, Rashidian M, Knoll M, Keliher E, Garrett S, et al. PD-L1 is an activation-independent marker of brown adipocytes. Nat Commun. 2017;8:647 pubmed publisher
    ..Here, the authors show that PD-L1 is expressed on brown adipocytes, does not change upon BAT activation, and that BAT volume in mice can be measured by PET-CT with a radiolabeled anti-PD-L1 antibody. ..