Mikael Pittet

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi Behavior of immune players in the tumor microenvironment
    Mikael J Pittet
    Center for Systems Biology and Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Curr Opin Oncol 21:53-9. 2009
  2. ncbi Angiotensin-converting enzyme inhibition prevents the release of monocytes from their splenic reservoir in mice with myocardial infarction
    Florian Leuschner
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, USA
    Circ Res 107:1364-73. 2010
  3. ncbi Intravital imaging
    Mikael J Pittet
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Cell 147:983-91. 2011
  4. ncbi In vivo imaging of T cell delivery to tumors after adoptive transfer therapy
    Mikael J Pittet
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Simches Research Building, 185 Cambridge Street, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 104:12457-61. 2007
  5. ncbi Monocytes link atherosclerosis and cancer
    Mikael J Pittet
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Eur J Immunol 41:2519-22. 2011
  6. ncbi The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions
    Matthias Nahrendorf
    Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    J Exp Med 204:3037-47. 2007
  7. ncbi Myeloperoxidase-rich Ly-6C+ myeloid cells infiltrate allografts and contribute to an imaging signature of organ rejection in mice
    Filip K Swirski
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
    J Clin Invest 120:2627-34. 2010
  8. ncbi Hybrid PET-optical imaging using targeted probes
    Matthias Nahrendorf
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 107:7910-5. 2010
  9. ncbi Hybrid in vivo FMT-CT imaging of protease activity in atherosclerosis with customized nanosensors
    Matthias Nahrendorf
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
    Arterioscler Thromb Vasc Biol 29:1444-51. 2009
  10. ncbi Monocyte subset dynamics in human atherosclerosis can be profiled with magnetic nano-sensors
    Moritz Wildgruber
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
    PLoS ONE 4:e5663. 2009

Research Grants

  1. Imaging of progenitor cells in tumor environments
    Mikael Pittet; Fiscal Year: 2004
  2. In vivo behavior of monocytes in resting and inflammatory conditions
    Mikael Pittet; Fiscal Year: 2011

Collaborators

Detail Information

Publications27

  1. ncbi Behavior of immune players in the tumor microenvironment
    Mikael J Pittet
    Center for Systems Biology and Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Curr Opin Oncol 21:53-9. 2009
    ..Yet, the precise role of these cells in situ remains vastly unknown. This review presents a new discovery effort that employs intravital imaging to study immune players directly in tissues...
  2. ncbi Angiotensin-converting enzyme inhibition prevents the release of monocytes from their splenic reservoir in mice with myocardial infarction
    Florian Leuschner
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, USA
    Circ Res 107:1364-73. 2010
    ..Monocytes recruited to ischemic myocardium originate from a reservoir in the spleen, and the release from their splenic niche relies on angiotensin (Ang) II signaling...
  3. ncbi Intravital imaging
    Mikael J Pittet
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Cell 147:983-91. 2011
    ....
  4. ncbi In vivo imaging of T cell delivery to tumors after adoptive transfer therapy
    Mikael J Pittet
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Simches Research Building, 185 Cambridge Street, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 104:12457-61. 2007
    ....
  5. ncbi Monocytes link atherosclerosis and cancer
    Mikael J Pittet
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Eur J Immunol 41:2519-22. 2011
    ..In this Viewpoint, we explore how monocytes, which are key constituents of the immune system, forge links between cardiovascular diseases and cancers...
  6. ncbi The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions
    Matthias Nahrendorf
    Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    J Exp Med 204:3037-47. 2007
    ....
  7. ncbi Myeloperoxidase-rich Ly-6C+ myeloid cells infiltrate allografts and contribute to an imaging signature of organ rejection in mice
    Filip K Swirski
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
    J Clin Invest 120:2627-34. 2010
    ..Thus, MPO imaging represents a potential alternative to the current invasive clinical standard by which transplants are monitored...
  8. ncbi Hybrid PET-optical imaging using targeted probes
    Matthias Nahrendorf
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 107:7910-5. 2010
    ..The findings also suggest that FMT can serve as a surrogate modality for the screening and development of radionuclide-based imaging agents...
  9. ncbi Hybrid in vivo FMT-CT imaging of protease activity in atherosclerosis with customized nanosensors
    Matthias Nahrendorf
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
    Arterioscler Thromb Vasc Biol 29:1444-51. 2009
    ....
  10. ncbi Monocyte subset dynamics in human atherosclerosis can be profiled with magnetic nano-sensors
    Moritz Wildgruber
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
    PLoS ONE 4:e5663. 2009
    ....
  11. ncbi Heterogeneous in vivo behavior of monocyte subsets in atherosclerosis
    Filip K Swirski
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Simches Research Building, 185 Cambridge Street, Boston, MA 02114, USA
    Arterioscler Thromb Vasc Biol 29:1424-32. 2009
    ..In this review we summarize recent advances of our understanding of the behavioral heterogeneity of monocytes during disease progression and outline emerging molecular imaging approaches to address key questions in the field...
  12. ncbi Identification of splenic reservoir monocytes and their deployment to inflammatory sites
    Filip K Swirski
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Science 325:612-6. 2009
    ..These observations uncover a role for the spleen as a site for storage and rapid deployment of monocytes and identify splenic monocytes as a resource that the body exploits to regulate inflammation...
  13. ncbi Ly-6Chi monocytes dominate hypercholesterolemia-associated monocytosis and give rise to macrophages in atheromata
    Filip K Swirski
    Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA
    J Clin Invest 117:195-205. 2007
    ..Conversely, the number of Ly-6C(lo) cells remained unaffected. Thus, we believe that Ly-6C(hi) monocytes represent a newly recognized component of the inflammatory response in experimental atherosclerosis...
  14. ncbi Labeling of immune cells for in vivo imaging using magnetofluorescent nanoparticles
    Mikael J Pittet
    Center for Molecular Imaging Research, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    Nat Protoc 1:73-9. 2006
    ..The present protocol focuses on T-cell labeling but can be used for labeling a variety of circulating cells...
  15. ncbi Dragon (repulsive guidance molecule b) inhibits IL-6 expression in macrophages
    Yin Xia
    Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    J Immunol 186:1369-76. 2011
    ..These results indicate that Dragon is an important negative regulator of IL-6 expression in immune cells and that Dragon-deficient mice may be a useful model for studying immune and inflammatory disorders...
  16. ncbi Impaired infarct healing in atherosclerotic mice with Ly-6C(hi) monocytosis
    Peter Panizzi
    Center for Systems Biology, Simches Research Building, Massachusetts General Hospital and Harvard Medical School Boston, Massachusetts 02114, USA
    J Am Coll Cardiol 55:1629-38. 2010
    ..The aim of this study was to test whether blood monocytosis in mice with atherosclerosis affects infarct healing...
  17. ncbi Monocyte accumulation in mouse atherogenesis is progressive and proportional to extent of disease
    Filip K Swirski
    Center for Molecular Imaging Research and Donald W. Reynolds Cardiovascular Clinical Research Center on Atherosclerosis at Harvard Medical School, Massachusetts General Hospital and Harvard Medical School, CNY 149, Charlestown, MA 02129, USA
    Proc Natl Acad Sci U S A 103:10340-5. 2006
    ..These results provide insights into mechanisms of atherogenesis and have implications for the duration of therapies directed at leukocyte recruitment...
  18. ncbi Self-assembled magnetic filter for highly efficient immunomagnetic separation
    David Issadore
    Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA
    Lab Chip 11:147-51. 2011
    ....
  19. ncbi Real-time assessment of inflammation and treatment response in a mouse model of allergic airway inflammation
    Virna Cortez-Retamozo
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    J Clin Invest 118:4058-66. 2008
    ..Both fluorescence-mediated tomography and fiberoptic bronchoscopy techniques have the potential to be translated into the clinic...
  20. ncbi Regulatory T cells reversibly suppress cytotoxic T cell function independent of effector differentiation
    Thorsten R Mempel
    CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Immunity 25:129-41. 2006
    ..Thus, T(reg) cells reversibly suppress CTL-mediated immunity by allowing acquisition of full effector potential but withholding the license to kill...
  21. ncbi Direct presentation of antigen by lymph node stromal cells protects against CD8 T-cell-mediated intestinal autoimmunity
    Fay C Magnusson
    Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, USA
    Gastroenterology 134:1028-37. 2008
    ..To determine the contribution of antigen-specific CD8 and CD4 T cells to the breakdown of the EGC network, we studied specific autoimmune targeting of an ectopic antigen expressed by EGCs...
  22. ncbi Imaging of molecular probe activity with Born-normalized fluorescence optical projection tomography
    Claudio Vinegoni
    Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Opt Lett 35:1088-90. 2010
    ....
  23. ncbi Behavior of endogenous tumor-associated macrophages assessed in vivo using a functionalized nanoparticle
    Antoine Leimgruber
    Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Neoplasia 11:459-68, 2 p following 468. 2009
    ..Noninvasive imaging can also be used to monitor TAM-depleting regimen quantitatively. Thus, AMTA680 or related cell-targeting agents represent appropriate injectable vehicles for in vivo analysis of the tumor microenvironment...
  24. ncbi Dual channel optical tomographic imaging of leukocyte recruitment and protease activity in the healing myocardial infarct
    Matthias Nahrendorf
    Center for Molecular Imaging Research, Massachusetts General Hospital, Boston, USA
    Circ Res 100:1218-25. 2007
    ..Spectrally resolved imaging agents allow for simultaneous assesment of key processes of in vivo cellular functions. Specifically, we show that in vivo FMT detects impaired healing in FXIII-/- mice...
  25. ncbi Regulation of T-cell migration and effector functions: insights from in vivo imaging studies
    Mikael J Pittet
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Simches Research Building, Boston, MA 02129, USA
    Immunol Rev 221:107-29. 2008
    ..In this review, we summarize recent insights on migration and effector functions of T cells, focusing on observations gained from their dynamic microscopic visualization in physiological tissue environments...
  26. ncbi Imaging in the era of molecular oncology
    Ralph Weissleder
    Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, USA
    Nature 452:580-9. 2008
    ....
  27. ncbi Improved in vivo whole-animal detection limits of green fluorescent protein-expressing tumor lines by spectral fluorescence imaging
    Jenny M Tam
    Center for Molecular Imaging Research, Massachusetts General Hospital, Boston, MA 02114, USA
    Mol Imaging 6:269-76. 2007
    ..These findings demonstrate the utility of the approach in detecting low levels of multiple fluorescent markers for whole-animal in vivo applications...

Research Grants3

  1. Imaging of progenitor cells in tumor environments
    Mikael Pittet; Fiscal Year: 2004
    ..abstract_text> ..
  2. In vivo behavior of monocytes in resting and inflammatory conditions
    Mikael Pittet; Fiscal Year: 2011
    ..Using novel in vivo experimental approaches we will attempt to identify key molecular players that define the biology of splenic monocytes and how they can be manipulated for new therapeutic approaches. ..