G B Pier

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi request reprint Application of vaccine technology to prevention of Pseudomonas aeruginosa infections
    Gerald Pier
    Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    Expert Rev Vaccines 4:645-56. 2005
  2. pmc Prophylactic and therapeutic efficacy of a fully human immunoglobulin G1 monoclonal antibody to Pseudomonas aeruginosa alginate in murine keratitis infection
    Tanweer Zaidi
    Department of Medicine, Channing Channing Laboratory, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Infect Immun 76:4720-5. 2008
  3. pmc Poly-N-acetylglucosamine expression by wild-type Yersinia pestis is maximal at mammalian, not flea, temperatures
    Pauline Yoong
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    MBio 3:e00217-12. 2012
  4. pmc Pseudomonas aeruginosa lipopolysaccharide: a major virulence factor, initiator of inflammation and target for effective immunity
    Gerald B Pier
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Int J Med Microbiol 297:277-95. 2007
  5. ncbi request reprint Biologic properties and vaccine potential of the staphylococcal poly-N-acetyl glucosamine surface polysaccharide
    Tomas Maira-Litran
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, 181 Longwood Avenue, Boston, MA 02115, USA
    Vaccine 22:872-9. 2004
  6. ncbi request reprint CFTR mutations and host susceptibility to Pseudomonas aeruginosa lung infection
    Gerald B Pier
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115 5804, USA
    Curr Opin Microbiol 5:81-6. 2002
  7. pmc Role of alginate O acetylation in resistance of mucoid Pseudomonas aeruginosa to opsonic phagocytosis
    G B Pier
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115 5804, USA
    Infect Immun 69:1895-901. 2001
  8. pmc Role of the cystic fibrosis transmembrane conductance regulator in innate immunity to Pseudomonas aeruginosa infections
    G B Pier
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115 5899, USA
    Proc Natl Acad Sci U S A 97:8822-8. 2000
  9. ncbi request reprint Human monoclonal antibodies to Pseudomonas aeruginosa alginate that protect against infection by both mucoid and nonmucoid strains
    Gerald B Pier
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 173:5671-8. 2004
  10. ncbi request reprint Salmonella typhi uses CFTR to enter intestinal epithelial cells
    G B Pier
    Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 393:79-82. 1998

Detail Information

Publications73

  1. ncbi request reprint Application of vaccine technology to prevention of Pseudomonas aeruginosa infections
    Gerald Pier
    Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    Expert Rev Vaccines 4:645-56. 2005
    ..aeruginosa infections...
  2. pmc Prophylactic and therapeutic efficacy of a fully human immunoglobulin G1 monoclonal antibody to Pseudomonas aeruginosa alginate in murine keratitis infection
    Tanweer Zaidi
    Department of Medicine, Channing Channing Laboratory, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Infect Immun 76:4720-5. 2008
    ..These findings indicate that MAb F429 could be useful as an additional therapeutic component for the treatment of P. aeruginosa keratitis...
  3. pmc Poly-N-acetylglucosamine expression by wild-type Yersinia pestis is maximal at mammalian, not flea, temperatures
    Pauline Yoong
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    MBio 3:e00217-12. 2012
    ..PNAG production by WT Y. pestis is maximal at mammalian and not insect vector temperatures, suggesting that this factor may have a role during mammalian infection...
  4. pmc Pseudomonas aeruginosa lipopolysaccharide: a major virulence factor, initiator of inflammation and target for effective immunity
    Gerald B Pier
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Int J Med Microbiol 297:277-95. 2007
    ..aeruginosa infection...
  5. ncbi request reprint Biologic properties and vaccine potential of the staphylococcal poly-N-acetyl glucosamine surface polysaccharide
    Tomas Maira-Litran
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, 181 Longwood Avenue, Boston, MA 02115, USA
    Vaccine 22:872-9. 2004
    ..Animal studies have shown purified PNAG can elicit protective immunity against both CoNS and S. aureus, suggesting its potential as a broadly protective vaccine for many clinically important strains of staphylococci...
  6. ncbi request reprint CFTR mutations and host susceptibility to Pseudomonas aeruginosa lung infection
    Gerald B Pier
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115 5804, USA
    Curr Opin Microbiol 5:81-6. 2002
    ..The function of CFTR in innate immunity to P. aeruginosa infection is multifactorial, with one key component being a specific ligand-receptor interaction between the protein and the microbe...
  7. pmc Role of alginate O acetylation in resistance of mucoid Pseudomonas aeruginosa to opsonic phagocytosis
    G B Pier
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115 5804, USA
    Infect Immun 69:1895-901. 2001
    ..O acetylation of alginate appears to be critical for P. aeruginosa resistance to host immune effectors in CF patients...
  8. pmc Role of the cystic fibrosis transmembrane conductance regulator in innate immunity to Pseudomonas aeruginosa infections
    G B Pier
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115 5899, USA
    Proc Natl Acad Sci U S A 97:8822-8. 2000
    ..The identification of CFTR as a receptor for bacterial pathogens could underlie the biology of CF lung disease and be the basis for the heterozygote advantage for carriers of mutant alleles of CFTR...
  9. ncbi request reprint Human monoclonal antibodies to Pseudomonas aeruginosa alginate that protect against infection by both mucoid and nonmucoid strains
    Gerald B Pier
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 173:5671-8. 2004
    ..aeruginosa and the results document that alginate is a target for the proper type of protective Ab even when expressed at low levels on phenotypically nonmucoid strains...
  10. ncbi request reprint Salmonella typhi uses CFTR to enter intestinal epithelial cells
    G B Pier
    Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 393:79-82. 1998
    ..typhi in the submucosa of Cftr wild-type mice than in deltaF508 heterozygous mice. We conclude that diminished levels of CFTR in heterozygotes may decrease susceptibility to typhoid fever...
  11. pmc Cystic fibrosis transmembrane conductance regulator is an epithelial cell receptor for clearance of Pseudomonas aeruginosa from the lung
    G B Pier
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115 5804, USA
    Proc Natl Acad Sci U S A 94:12088-93. 1997
    ..aeruginosa internalization in vivo, leading to increased bacterial lung burdens. CFTR clears P. aeruginosa from the lung, indicating a direct connection between mutations in CFTR and the clinical consequences of CF...
  12. ncbi request reprint Transgenic cystic fibrosis mice exhibit reduced early clearance of Pseudomonas aeruginosa from the respiratory tract
    T H Schroeder
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 166:7410-8. 2001
    ..Thus, transgenic CF mice exhibit decreased clearance of P. aeruginosa and increased bacterial burdens in the lung, substantiating a key role for CFTR-mediated bacterial ingestion in lung clearance of P. aeruginosa...
  13. pmc The ica locus of Staphylococcus epidermidis encodes production of the capsular polysaccharide/adhesin
    D McKenney
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115 5899, USA
    Infect Immun 66:4711-20. 1998
    ..We conclude that the ica locus encodes production of PS/A and that the properties of S. epidermidis associated with initial bacterial adherence, biofilm formation, and intercellular adhesion can be correlated with elaboration of PS/A...
  14. ncbi request reprint Epithelial cell contact-induced alterations in Salmonella enterica serovar Typhi lipopolysaccharide are critical for bacterial internalization
    J B Lyczak
    The Channing Laboratory, Brigham and Women s Hospital, 181 Longwood Avenue, Boston, MA 02115, USA
    Cell Microbiol 3:763-72. 2001
    ..aeruginosa LPS core, is a ligand mediating internalization of bacteria by epithelial cells, and that exposure of this ligand on wild-type Typhi is induced by the bacteria's interaction with host cells...
  15. pmc Production and characterization of a set of mouse-human chimeric immunoglobulin G (IgG) subclass and IgA monoclonal antibodies with identical variable regions specific for Pseudomonas aeruginosa serogroup O6 lipopolysaccharide
    M J Preston
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Infect Immun 66:4137-42. 1998
    ..aeruginosa and will be critical for defining the optimal formulation of either a vaccine for active immunization or a polyclonal intravenous IgG or monoclonal antibody cocktail for passive immunotherapy...
  16. pmc Contribution of proteases and LasR to the virulence of Pseudomonas aeruginosa during corneal infections
    M J Preston
    Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Infect Immun 65:3086-90. 1997
    ..However, the lack of virulence of the LasA-deficient strains cannot be ascribed with certainty to the deficiency of LasA from the available data...
  17. ncbi request reprint Exploitation of syndecan-1 shedding by Pseudomonas aeruginosa enhances virulence
    P W Park
    Division of Newborn Medicine, Department of Pediatrics, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Nature 411:98-102. 2001
    ..Our findings uncover a pathogenetic mechanism in which a host response to tissue injury-syndecan-1 shedding-is exploited to enhance microbial virulence apparently by modulating host defences...
  18. ncbi request reprint Structure of an antigenic teichoic acid shared by clinical isolates of Enterococcus faecalis and vancomycin-resistant Enterococcus faecium
    Y Wang
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115 5804, USA
    Carbohydr Res 316:155-60. 1999
    ..It was composed of glucose, glycerol, and phosphate as determined by chemical and GC-MS analysis. The repeating-unit structure was elucidated by a series of 1H, 13C, and 31P NMR spectroscopy to be the following: [formula: see text]..
  19. pmc Prophylactic and therapeutic efficacy of antibodies to a capsular polysaccharide shared among vancomycin-sensitive and -resistant enterococci
    J Huebner
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Infect Immun 68:4631-6. 2000
    ..These data indicate that CP antigens from enterococci are potential targets of protective antibodies and that these antibodies may be useful for prophylaxis and treatment of enterococcal infections...
  20. pmc Resistance to Pseudomonas aeruginosa chronic lung infection requires cystic fibrosis transmembrane conductance regulator-modulated interleukin-1 (IL-1) release and signaling through the IL-1 receptor
    Nina Reiniger
    Channing Laboratory, Brigham and Women s Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA
    Infect Immun 75:1598-608. 2007
    ..Overall, rapid IL-1 release and signaling through IL-1R represent key steps in the innate immune response to P. aeruginosa infection, and this process is deficient in cells lacking functional CFTR...
  21. ncbi request reprint Conserved and variable structural features in the lipopolysaccharide of Pseudomonas aeruginosa
    Yuriy A Knirel
    N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, Moscow 119991, Russia
    J Endotoxin Res 12:324-36. 2006
    ..Data on biosynthesis, genetics and serology of the lipopolysaccharide isolated from various P. aeruginosa O-serogroups are discussed in relation to the chemical structures...
  22. ncbi request reprint Inflammatory markers of lung disease in adult patients with cystic fibrosis
    Hara Levy
    Division of Pulmonary Medicine, Children s Hospital Boston, Boston, Massachusetts, USA
    Pediatr Pulmonol 42:256-62. 2007
    ..We hypothesized that levels of serum biomarkers would correlate with clinical course of CF as defined by pulmonary function testing (FEV1)...
  23. pmc Comparative antibody-mediated phagocytosis of Staphylococcus epidermidis cells grown in a biofilm or in the planktonic state
    Nuno Cerca
    Centro de Engenharia Biólogica, Universidade do Minho, Braga, Portugal
    Infect Immun 74:4849-55. 2006
    ....
  24. pmc Characterization of the opsonic and protective activity against Staphylococcus aureus of fully human monoclonal antibodies specific for the bacterial surface polysaccharide poly-N-acetylglucosamine
    Casie Kelly-Quintos
    Harvard Medical School, 181 Longwood Ave, Boston, MA 02115, USA
    Infect Immun 74:2742-50. 2006
    ....
  25. pmc A live-attenuated Pseudomonas aeruginosa vaccine elicits outer membrane protein-specific active and passive protection against corneal infection
    Tanweer S Zaidi
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
    Infect Immun 74:975-83. 2006
    ..aeruginosa corneal infections caused by diverse LPS serogroups...
  26. ncbi request reprint Structures of the core oligosaccharide and O-units in the R- and SR-type lipopolysaccharides of reference strains of Pseudomonas aeruginosa O-serogroups
    Olga V Bystrova
    N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
    FEMS Immunol Med Microbiol 46:85-99. 2006
    ..The data obtained link together the known biosynthesis pathways, genetics and serology of the P. aeruginosa lipopolysaccharide...
  27. ncbi request reprint The role of epitope specificity in the human opsonic antibody response to the staphylococcal surface polysaccharide poly N-acetyl glucosamine
    Casie Kelly-Quintos
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    J Infect Dis 192:2012-9. 2005
    ..We investigated the human antibody response and specificity of binding and opsonic antibodies for different epitopes on PNAG in serum samples from patients with cystic fibrosis (CF) colonized and not colonized with Staphylococcus aureus...
  28. pmc Intraspecies and temperature-dependent variations in susceptibility of Yersinia pestis to the bactericidal action of serum and to polymyxin B
    Andrey P Anisimov
    State Research Center for Applied Microbiology, Obolensk, Moscow Region 142279, Russia
    Infect Immun 73:7324-31. 2005
    ..The NHS resistance correlated with an elevated content of N-acetylglucosamine in the LPS. Structural variation in the LPS of Y. pestis correlates with the organism's ability to resist innate immunity in both fleas and mammals...
  29. ncbi request reprint Promises and pitfalls of Pseudomonas aeruginosa lipopolysaccharide as a vaccine antigen
    Gerald B Pier
    Department of Medicine, Channing Laboratory, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Carbohydr Res 338:2549-56. 2003
    ....
  30. pmc Intranasal immunization with heterologously expressed polysaccharide protects against multiple Pseudomonas aeruginosa infections
    Antonio DiGiandomenico
    Department of Microbiology, University of Virginia Health System, 1300 Jefferson Avenue, Charlottesville, VA 22908, USA
    Proc Natl Acad Sci U S A 104:4624-9. 2007
    ....
  31. ncbi request reprint Effect of deletion of the lpxM gene on virulence and vaccine potential of Yersinia pestis in mice
    Andrey P Anisimov
    State Research Center for Applied Microbiology and Biotechnology, Obolensk 142279, Moscow Region, Russia
    J Med Microbiol 56:443-53. 2007
    ..These studies suggest there is little impact from lipid A modifications on the virulence of Y. pestis strains but there are potential improvements in the protective properties in attenuated vaccine strains...
  32. pmc Protection against Escherichia coli infection by antibody to the Staphylococcus aureus poly-N-acetylglucosamine surface polysaccharide
    Nuno Cerca
    Channing Laboratory, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:7528-33. 2007
    ..coli strains expressing PNAG. PNAG expression by both Gram-negative and Gram-positive organisms could make this antigen a conserved vaccine target for multiple pathogenic species of bacteria...
  33. pmc ClpXP proteases positively regulate alginate overexpression and mucoid conversion in Pseudomonas aeruginosa
    Dongru Qiu
    Department of Biochemistry and Microbiology, Joan C Edwards School of Medicine at Marshall University, Huntington, WV 25755 9320, USA
    Microbiology 154:2119-30. 2008
    ..The ClpXP and ClpP2 proteins appear to be part of a proteolytic network that degrades the cytoplasmic portion of truncated MucA proteins to release the sequestered AlgU, which drives alginate biosynthesis...
  34. doi request reprint Dropping acid to help cystic fibrosis
    Gerald B Pier
    Nat Med 14:367-9. 2008
  35. pmc Disruption of CFTR-dependent lipid rafts reduces bacterial levels and corneal disease in a murine model of Pseudomonas aeruginosa keratitis
    Tanweer Zaidi
    Channing Laboratory and Hematology Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Invest Ophthalmol Vis Sci 49:1000-9. 2008
    ....
  36. pmc Wall teichoic acids are dispensable for anchoring the PNAG exopolysaccharide to the Staphylococcus aureus cell surface
    Marta Vergara-Irigaray
    Laboratory of Microbial Biofilms, Instituto de Agrobiotecnologia, Universidad Pública de Navarra CSIC Gobierno de Navarra, 31006 Pamplona, Spain
    Microbiology 154:865-77. 2008
    ..aureus had little effect on PNAG production or anchoring to the cell surface, but did affect the biofilm-forming capacity, cell aggregative behaviour and the temperature sensitivity/stability of S. aureus...
  37. pmc Mucosal damage and neutropenia are required for Candida albicans dissemination
    Andrew Y Koh
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Pathog 4:e35. 2008
    ..albicans disease as well as identifying virulence factors that are necessary for fungal GI colonization and dissemination. The model may also prove valuable for evaluating therapies to control C. albicans infections...
  38. pmc Airway epithelial control of Pseudomonas aeruginosa infection in cystic fibrosis
    Victoria L Campodónico
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Trends Mol Med 14:120-33. 2008
    ....
  39. doi request reprint Vaccines and immunotherapy against Pseudomonas aeruginosa
    Gerd Doring
    Institute of Medical Microbiology and Hygiene, University of Tubingen, D 72074 Tubingen, Germany
    Vaccine 26:1011-24. 2008
    ..Finally, the inherent problem of testing P. aeruginosa candidate vaccines in patient populations is discussed...
  40. ncbi request reprint Relationship of the lipopolysaccharide structure of Yersinia pestis to resistance to antimicrobial factors
    Yuriy A Knirel
    N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
    Adv Exp Med Biol 603:88-96. 2007
    ..Resistance to polymyxin B also requires a high content of the cationic sugar, 4-amino-4-deoxy-L-arabinose, in lipid A...
  41. pmc Host resistance to lung infection mediated by major vault protein in epithelial cells
    Michael P Kowalski
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Science 317:130-2. 2007
    ..MVP was also essential for optimal epithelial cell internalization and clearance of P. aeruginosa. These results suggest that MVP makes a substantial contribution to epithelial cell-mediated resistance to infection...
  42. pmc Molecular basis for preferential protective efficacy of antibodies directed to the poorly acetylated form of staphylococcal poly-N-acetyl-beta-(1-6)-glucosamine
    Nuno Cerca
    Channing Laboratory, Brigham and Women s Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA
    Infect Immun 75:3406-13. 2007
    ..Retention of dPNAG on the surface of S. aureus is key to increased survival during bacteremia and also provides a molecular mechanism explaining the superior opsonic and protective activity of antibody to dPNAG...
  43. pmc Predictors of mucoid Pseudomonas colonization in cystic fibrosis patients
    Hara Levy
    Division of Pulmonary Medicine, Children s Hospital, Boston, Massachusetts, USA
    Pediatr Pulmonol 43:463-71. 2008
    ..aeruginosa acquisition...
  44. ncbi request reprint Is Pseudomonas aeruginosa exotoxin A a good carrier protein for conjugate vaccines?
    Gerald B Pier
    Hum Vaccin 3:39-40; author reply 41. 2007
  45. pmc Poly-N-acetylglucosamine production in Staphylococcus aureus is essential for virulence in murine models of systemic infection
    Andrea Kropec
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Infect Immun 73:6868-76. 2005
    ..Thus, PNAG confers on S. aureus resistance to killing mediated by these innate host immune mediators. Overall, PNAG production by S. aureus appears to be a critical virulence factor as assessed in murine models of systemic infection...
  46. pmc Influence of cystic fibrosis transmembrane conductance regulator on gene expression in response to Pseudomonas aeruginosa infection of human bronchial epithelial cells
    Nina Reiniger
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
    Infect Immun 73:6822-30. 2005
    ..Cytokine effectors of innate immunity to P. aeruginosa were found to be positively influenced by the presence of WT CFTR, indicating a role in resistance to P. aeruginosa infection...
  47. ncbi request reprint Structure of the lipopolysaccharide of Pseudomonas aeruginosa O-12 with a randomly O-acetylated core region
    Olga V Bystrova
    ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia
    Carbohydr Res 338:1895-905. 2003
    ..The core was found to be randomly O-acetylated, most O-acetyl groups being located on the terminal rhamnose residue of the outer core region...
  48. ncbi request reprint Structure of the biological repeating unit of the O-antigen of Pseudomonas aeruginosa immunotype 4 containing both 2-acetamido-2,6-dideoxy-D-glucose and 2-acetamido-2,6-dideoxy-D-galactose
    Olga V Bystrova
    N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation
    Carbohydr Res 338:1801-6. 2003
    ..These data define the structure of the biological repeating unit of the O-antigen...
  49. ncbi request reprint Pseudomonas aeruginosa-induced apoptosis is defective in respiratory epithelial cells expressing mutant cystic fibrosis transmembrane conductance regulator
    Carolyn L Cannon
    Channing Laboratory, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Am J Respir Cell Mol Biol 29:188-97. 2003
    ..aeruginosa infection. Prompt apoptosis of infected epithelial cells may be critical for clearance of P. aeruginosa, and CFTR-associated defects in apoptosis may contribute to the pathogenesis of the lung disease in cystic fibrosis...
  50. pmc Construction and characterization of a Pseudomonas aeruginosa mucoid exopolysaccharide-alginate conjugate vaccine
    Christian Theilacker
    Channing Laboratory, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115 5804, USA
    Infect Immun 71:3875-84. 2003
    ....
  51. ncbi request reprint Identification of a 5-nucleotide sequence that controls expression of the ica locus in Staphylococcus aureus and characterization of the DNA-binding properties of IcaR
    Kimberly K Jefferson
    The Channing Laboratory, Brigham and Women s Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA
    Mol Microbiol 48:889-99. 2003
    ..aureus ica locus...
  52. pmc Protection against fatal Pseudomonas aeruginosa pneumonia in mice after nasal immunization with a live, attenuated aroA deletion mutant
    Gregory P Priebe
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Infect Immun 71:1453-61. 2003
    ..aeruginosa lung infections, that such immunity can be elicited by using aroA deletion mutants, and that a multivalent P. aeruginosa vaccine composed of aroA deletion mutants of multiple serogroups holds significant promise...
  53. pmc Hypersusceptibility of cystic fibrosis mice to chronic Pseudomonas aeruginosa oropharyngeal colonization and lung infection
    Fadie T Coleman
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:1949-54. 2003
    ..aeruginosa colonization and infection and can be used for evaluations of lung pathophysiology, bacterial virulence, and development of therapies aimed at treating CF lung disease...
  54. pmc Salmonella enterica serovar typhi modulates cell surface expression of its receptor, the cystic fibrosis transmembrane conductance regulator, on the intestinal epithelium
    Jeffrey B Lyczak
    The Channing Laboratory, Brigham and Women s Hospital, and Harvard Medical School, Boston, Massachusetts 02115, USA
    Infect Immun 70:6416-23. 2002
    ..This could be a key, early step in the infectious process leading to typhoid fever...
  55. pmc Immunochemical properties of the staphylococcal poly-N-acetylglucosamine surface polysaccharide
    Tomas Maira-Litran
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Boston, Massachusetts, USA
    Infect Immun 70:4433-40. 2002
    ..These results further clarify the chemical structure of PS/A and help to differentiate it from PIA on the basis of immunogenicity, molecular size, and solubility...
  56. pmc CFTR is a pattern recognition molecule that extracts Pseudomonas aeruginosa LPS from the outer membrane into epithelial cells and activates NF-kappa B translocation
    Torsten H Schroeder
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 99:6907-12. 2002
    ..CFTR serves as a critical endocytic PRM in the lung epithelium, coordinating the effective innate immune response to P. aeruginosa infection...
  57. ncbi request reprint Structural studies on the core and the O-polysaccharide repeating unit of Pseudomonas aeruginosa immunotype 1 lipopolysaccharide
    Olga V Bystrova
    N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
    Eur J Biochem 269:2194-203. 2002
    ..aeruginosa O6, which is closely related to immunotype 1, by transfer of d-QuiNAc-1-P to undecaprenyl phosphate followed by synthesis of the repeating O-antigen tetrasaccharide...
  58. pmc Lung infections associated with cystic fibrosis
    Jeffrey B Lyczak
    Channing Laboratory, Brigham and Women s Hospital, Harvard Medical School, Children s Hospital, Boston, MA 02115, USA
    Clin Microbiol Rev 15:194-222. 2002
    ....
  59. pmc Transcription of quorum-sensing system genes in clinical and environmental isolates of Pseudomonas aeruginosa
    Ségolène Cabrol
    INSERM EMI 9933, Epidémiologie de la Résistance aux Anti infectieux, and AP HP Groupe Hospitalier Bichat Claude Bernard, 75877 Paris Cedex 18, France
    J Bacteriol 185:7222-30. 2003
    ....
  60. ncbi request reprint Localization of cystic fibrosis transmembrane conductance regulator to lipid rafts of epithelial cells is required for Pseudomonas aeruginosa-induced cellular activation
    Michael P Kowalski
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA
    J Immunol 172:418-25. 2004
    ..aeruginosa infection. Such signaling is needed for the coordination of innate immunity to P. aeruginosa lung infection, a process that is defective in CF...
  61. pmc Comparative opsonic and protective activities of Staphylococcus aureus conjugate vaccines containing native or deacetylated Staphylococcal Poly-N-acetyl-beta-(1-6)-glucosamine
    Tomas Maira-Litran
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
    Infect Immun 73:6752-62. 2005
    ..aureus infection, including capsular polysaccharide types 5 and 8 and an untypable strain...
  62. pmc Nonmucoid Pseudomonas aeruginosa expresses alginate in the lungs of patients with cystic fibrosis and in a mouse model
    Alessandra Bragonzi
    Institute of Medical Microbiology and Hygiene, Universitatsklinikum Tubingen, Germany
    J Infect Dis 192:410-9. 2005
    ..We investigated the kinetics, impact of environmental signals, and genetics of P. aeruginosa alginate expression in a mouse model and in patients with CF...
  63. pmc Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT colorimetry
    Nuno Cerca
    Centro de Engenharia Biólogica, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    J Antimicrob Chemother 56:331-6. 2005
    ..To quantitatively compare the antibiotic susceptibility of biofilms formed by the coagulase-negative staphylococci (CoNS) Staphylococcus epidermidis and Staphylococcus haemolyticus with the susceptibility of planktonic cultures...
  64. pmc The relationship between inhibition of bacterial adhesion to a solid surface by sub-MICs of antibiotics and subsequent development of a biofilm
    Nuno Cerca
    Centro de Engenharia Biólogica, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Res Microbiol 156:650-5. 2005
    ..These results demonstrate that assays evaluating the inhibition of initial adherence to medical surfaces cannot fully predict the effect on inhibition of biofilm formation...
  65. pmc Use of confocal microscopy to analyze the rate of vancomycin penetration through Staphylococcus aureus biofilms
    Kimberly K Jefferson
    Channing Laboratory, Brigham and Women s Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA
    Antimicrob Agents Chemother 49:2467-73. 2005
    ..We also investigated the role of poly-N-acetylglucosamine, an important component of the S. aureus biofilm matrix, and found that its production was not involved in the observed decrease in the rate of vancomycin penetration...
  66. pmc Virulence of Pseudomonas aeruginosa in a murine model of gastrointestinal colonization and dissemination in neutropenia
    Andrew Y Koh
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, 181 Longwood Ave, Boston, MA 02115, USA
    Infect Immun 73:2262-72. 2005
    ..aeruginosa pathogenesis can be studied in this model, which should prove useful for evaluating pathogenesis, therapies, and associated means to control P. aeruginosa nosocomial infections...
  67. ncbi request reprint Temperature-dependent variations and intraspecies diversity of the structure of the lipopolysaccharide of Yersinia pestis
    Yuriy A Knirel
    N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia
    Biochemistry 44:1731-43. 2005
    ..pestis and epidemiologic means to detect, classify, control and respond to Y. pestis infections...
  68. pmc Hypoxia increases corneal cell expression of CFTR leading to increased Pseudomonas aeruginosa binding, internalization, and initiation of inflammation
    Tanweer Zaidi
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Invest Ophthalmol Vis Sci 45:4066-74. 2004
    ....
  69. pmc The galU Gene of Pseudomonas aeruginosa is required for corneal infection and efficient systemic spread following pneumonia but not for infection confined to the lung
    Gregory P Priebe
    Channing Laboratory, Brigham and Women s Hospital, 181 Longwood Ave, Boston, MA 02115, USA
    Infect Immun 72:4224-32. 2004
    ..Host defenses in the lung appear to be insufficient to control infection with LPS-rough P. aeruginosa when local bacterial levels are high...
  70. pmc The teicoplanin-associated locus regulator (TcaR) and the intercellular adhesin locus regulator (IcaR) are transcriptional inhibitors of the ica locus in Staphylococcus aureus
    Kimberly K Jefferson
    Channing Laboratory, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 186:2449-56. 2004
    ..In summary, TcaR appeared to be a weak negative regulator of transcription of the ica locus, whereas IcaR was a strong negative regulator, and in their absence PNAG production and biofilm formation were enhanced...
  71. ncbi request reprint Assessment of the role of antibiotics and enterococcal virulence factors in a mouse model of extraintestinal translocation
    Wolfgang A Krueger
    Department of Anaesthesiology and Intensive Care, Tubingen University Hospital, Germany
    Crit Care Med 32:467-71. 2004
    ..To study the relative contribution of antibiotics and bacterial virulence factors in the process of translocation of Enterococcus faecalis from the gut to extraintestinal organs...
  72. pmc Construction and characterization of a live, attenuated aroA deletion mutant of Pseudomonas aeruginosa as a candidate intranasal vaccine
    Gregory P Priebe
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Infect Immun 70:1507-17. 2002
    ..This live, attenuated P. aeruginosa strain PAO1 Delta aroA appears to be safe for potential use as an intranasal vaccine and elicits high titers of opsonic antibodies against multiple strains of the P. aeruginosa O2/O5 serogroup...
  73. ncbi request reprint Meeting summary: possibilities for active and passive vaccination against opportunistic infections
    Richard I Walker
    Vaccine 22:801-4. 2004