Norbert Perrimon

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi request reprint Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays
    Meghana M Kulkarni
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nat Methods 3:833-8. 2006
  2. ncbi request reprint Matter arising: off-targets and genome-scale RNAi screens in Drosophila
    Norbert Perrimon
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts USA
    Fly (Austin) 1:1-5. 2007
  3. pmc PAPTi: a peptide aptamer interference toolkit for perturbation of protein-protein interaction networks
    Johannes T H Yeh
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Sci Rep 3:1156. 2013
  4. pmc Of flies and men: insights on organismal metabolism from fruit flies
    Akhila Rajan
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    BMC Biol 11:38. 2013
  5. pmc Signaling mechanisms controlling cell fate and embryonic patterning
    Norbert Perrimon
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cold Spring Harb Perspect Biol 4:a005975. 2012
  6. pmc A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila
    Ramanuj DasGupta
    New York University School of Medicine Cancer Institute, Department of Pharmacology, First Avenue, New York, NY 10016, USA
    Genome Biol 8:R203. 2007
  7. pmc False negative rates in Drosophila cell-based RNAi screens: a case study
    Matthew Booker
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    BMC Genomics 12:50. 2011
  8. pmc An integrative approach to ortholog prediction for disease-focused and other functional studies
    Yanhui Hu
    Drosophila RNAi Screening Center, Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    BMC Bioinformatics 12:357. 2011
  9. pmc Using iterative cluster merging with improved gap statistics to perform online phenotype discovery in the context of high-throughput RNAi screens
    Zheng Yin
    Center for Bioinformatics, The Methodist Hospital Research Institute and Weill Cornell College of Medicine, 6565 Fannin Street, Houston, TX 77030, USA
    BMC Bioinformatics 9:264. 2008
  10. ncbi request reprint Drug-target identification in Drosophila cells: combining high-throughout RNAi and small-molecule screens
    Norbert Perrimon
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Drug Discov Today 12:28-33. 2007

Research Grants

  1. High-throughput screening assay development for axonopathic neurodegeration
    Norbert Perrimon; Fiscal Year: 2006
  2. Characterization of the Insulin to Autophagy Pathway in Muscles
    Norbert Perrimon; Fiscal Year: 2010
  3. ROLES OF HEPARAN SULFATE PROTEOGLYCANS IN SIGNALING
    Norbert Perrimon; Fiscal Year: 2000
  4. Viral Immunity in Drosophila
    Norbert Perrimon; Fiscal Year: 2007
  5. Functional Genomic analysis using RNAi screen in Drosophila
    Norbert Perrimon; Fiscal Year: 2007
  6. Network Topology in Cell Signaling
    Norbert Perrimon; Fiscal Year: 2007
  7. Host factors involved in bacterial pathogenesis
    Norbert Perrimon; Fiscal Year: 2008
  8. Network Topology in Cell Signaling
    Norbert Perrimon; Fiscal Year: 2008
  9. Functional Genomic analysis using RNAi screen in Drosophila
    Norbert Perrimon; Fiscal Year: 2008
  10. Drosophila Transgenic RNAi Resource Project
    Norbert Perrimon; Fiscal Year: 2008

Collaborators

Detail Information

Publications114 found, 100 shown here

  1. ncbi request reprint Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays
    Meghana M Kulkarni
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nat Methods 3:833-8. 2006
    ..Although a full appreciation of all causes of false positive errors remains to be determined, we suggest simple guidelines to help ensure high-quality information from RNAi high-throughput screens...
  2. ncbi request reprint Matter arising: off-targets and genome-scale RNAi screens in Drosophila
    Norbert Perrimon
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts USA
    Fly (Austin) 1:1-5. 2007
    ..Lastly, we summarize steps taken by the DRSC to redress that situation and include a set of recommendations to observe in future RNAi screens...
  3. pmc PAPTi: a peptide aptamer interference toolkit for perturbation of protein-protein interaction networks
    Johannes T H Yeh
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Sci Rep 3:1156. 2013
    ..Altogether, these studies demonstrate the power of the PAPTi approach to dissect specific PPI interactions within signaling networks...
  4. pmc Of flies and men: insights on organismal metabolism from fruit flies
    Akhila Rajan
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    BMC Biol 11:38. 2013
    ....
  5. pmc Signaling mechanisms controlling cell fate and embryonic patterning
    Norbert Perrimon
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cold Spring Harb Perspect Biol 4:a005975. 2012
    ..They also show how feedback loops and transcriptional cascades are part of the logic of developmental regulation...
  6. pmc A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila
    Ramanuj DasGupta
    New York University School of Medicine Cancer Institute, Department of Pharmacology, First Avenue, New York, NY 10016, USA
    Genome Biol 8:R203. 2007
    ..Furthermore, we investigate other factors that may influence the outcome of such screens, including cell-type specificity, robustness of reporters, and assay normalization, which determine the efficacy of RNAi-knockdown of target genes...
  7. pmc False negative rates in Drosophila cell-based RNAi screens: a case study
    Matthew Booker
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    BMC Genomics 12:50. 2011
    ..Whereas false positive results associated with RNAi reagents has been a matter of extensive study, the issue of false negatives has received less attention...
  8. pmc An integrative approach to ortholog prediction for disease-focused and other functional studies
    Yanhui Hu
    Drosophila RNAi Screening Center, Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    BMC Bioinformatics 12:357. 2011
    ..Several tools for predicting orthologous gene relationships are available. However, these tools can give different results and identification of predicted orthologs is not always straightforward...
  9. pmc Using iterative cluster merging with improved gap statistics to perform online phenotype discovery in the context of high-throughput RNAi screens
    Zheng Yin
    Center for Bioinformatics, The Methodist Hospital Research Institute and Weill Cornell College of Medicine, 6565 Fannin Street, Houston, TX 77030, USA
    BMC Bioinformatics 9:264. 2008
    ..Arbitrarily extracted information causes biased analysis, while combining the complete existing datasets with each new image is intractable in high-throughput screens...
  10. ncbi request reprint Drug-target identification in Drosophila cells: combining high-throughout RNAi and small-molecule screens
    Norbert Perrimon
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Drug Discov Today 12:28-33. 2007
    ..We then illustrate how the combination of RNAi and small-molecule HTS can lead to effective identification of targets in drug discovery...
  11. pmc In vivo RNAi: today and tomorrow
    Norbert Perrimon
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cold Spring Harb Perspect Biol 2:a003640. 2010
    ..Further, we discuss the applications of RNAi technologies to crop improvement, pest control and RNAi therapeutics, thus providing an appreciation of the potential for phenomenal applications of RNAi to agriculture and medicine...
  12. pmc Applications of high-throughput RNA interference screens to problems in cell and developmental biology
    Norbert Perrimon
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02175, USA
    Genetics 175:7-16. 2007
    ..Finally, we discuss emerging technological advances that will extend RNAi-based screening methods...
  13. ncbi request reprint Functional genomic analysis of the Wnt-wingless signaling pathway
    Ramanuj DasGupta
    Department of Genetics, Howard Hughes Medical Institute HHMI, Harvard Medical School, New Research Building, No 339, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Science 308:826-33. 2005
    ..High-throughput RNAi screens in cultured cells, followed by functional analyses in model organisms, prove to be a rapid means of identifying regulators of signaling pathways implicated in development and disease...
  14. doi request reprint RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance
    Jianwu Bai
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Development 135:1439-49. 2008
    ..Finally, we discuss how Drosophila primary cells can be manipulated to develop cell-based assays to model human diseases for RNAi and small-molecule screens...
  15. pmc Hierarchical rules for Argonaute loading in Drosophila
    Benjamin Czech
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Mol Cell 36:445-56. 2009
    ....
  16. pmc A genome-scale shRNA resource for transgenic RNAi in Drosophila
    Jian Quan Ni
    Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, Boston, Massachusetts, USA
    Nat Methods 8:405-7. 2011
    ..We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource...
  17. ncbi request reprint Function of the ETS transcription factor Yan in border cell migration
    Markus Schober
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Development 132:3493-504. 2005
    ..Our results suggest that Yan acts as a pivotal link between signal transduction, cell adhesion and invasive cell migration in Drosophila border cells...
  18. pmc Functional screening identifies miR-315 as a potent activator of Wingless signaling
    Serena J Silver
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:18151-6. 2007
    ..In summary, activity-based screening can selectively identify miRNAs whose deregulation can lead to interpretable phenotypic consequences...
  19. pmc Protein complex-based analysis framework for high-throughput data sets
    Arunachalam Vinayagam
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Sci Signal 6:rs5. 2013
    ..The analysis predicted that the Brahma complex participated in the insulin response...
  20. pmc Stringent analysis of gene function and protein-protein interactions using fluorescently tagged genes
    Ralph A Neumüller
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genetics 190:931-40. 2012
    ..Finally, we show that fluorescent traps, paired with recombinant nanobodies and mass spectrometry, allow the study of endogenous protein complexes in Drosophila...
  21. pmc Synergy between bacterial infection and genetic predisposition in intestinal dysplasia
    Yiorgos Apidianakis
    Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 106:20883-8. 2009
    ..Assessment of progenitor cell responses to pathogenic intestinal bacteria could provide a measure of predisposition for apoptotic enterocyte-assisted intestinal dysplasias in humans...
  22. pmc The Hippo tumor suppressor pathway regulates intestinal stem cell regeneration
    Phillip Karpowicz
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Development 137:4135-45. 2010
    ..These findings demonstrate a cell-autonomous role for the Hippo pathway in SCs, and have implications for understanding the role of this pathway in tumorigenesis and cancer stem cells...
  23. pmc A Drosophila resource of transgenic RNAi lines for neurogenetics
    Jian Quan Ni
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia 20147, USA
    Genetics 182:1089-100. 2009
    ....
  24. ncbi request reprint Drosophila RNAi screen reveals CD36 family member required for mycobacterial infection
    Jennifer A Philips
    Department of Genetics, Harvard Medical School, 77 Louis Pasteur Avenue, Boston, MA 02115, USA
    Science 309:1251-3. 2005
    ..fortuitum, which suggests a conserved role for class B SRs in pattern recognition and innate immunity...
  25. pmc Integrating protein-protein interaction networks with phenotypes reveals signs of interactions
    Arunachalam Vinayagam
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
    Nat Methods 11:94-9. 2014
    ....
  26. pmc Sarcomere formation occurs by the assembly of multiple latent protein complexes
    Yanning Rui
    Department of Genetics, Harvard Medical School, Boston, MA, USA
    PLoS Genet 6:e1001208. 2010
    ..Thus, sarcomere formation occurs by the coordinated assembly of multiple latent protein complexes, as opposed to sequential assembly...
  27. pmc Dynamic switch of negative feedback regulation in Drosophila Akt-TOR signaling
    Lutz Kockel
    Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Genet 6:e1000990. 2010
    ..These results illustrate how the Akt-TOR pathway dynamically adapts the routing of negative feedback in response to the activity load of its signaling circuit in vivo...
  28. pmc Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes
    Michele Markstein
    Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Genet 40:476-83. 2008
    ..The generality of our approach makes it adaptable to other model systems to identify and modify loci for optimal transgene expression...
  29. pmc Cross-species RNAi rescue platform in Drosophila melanogaster
    Shu Kondo
    Harvard Medical School, Department of Genetics, New Research Building RM 339, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Genetics 183:1165-73. 2009
    ..melanogaster, both in cell culture and in vivo. Altogether, the tools and method that we have developed provide a gold standard for validation of Drosophila RNAi experiments...
  30. ncbi request reprint The roles of JAK/STAT signaling in Drosophila immune responses
    Herve Agaisse
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Immunol Rev 198:72-82. 2004
    ....
  31. pmc Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling
    Adam A Friedman
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Sci Signal 4:rs10. 2011
    ....
  32. pmc A regulatory network of Drosophila germline stem cell self-renewal
    Dong Yan
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 28:459-73. 2014
    ..Altogether, our study provides a resource that will help to further dissect the networks underlying stem cell self-renewal. ..
  33. ncbi request reprint Genome-wide RNAi screen for host factors required for intracellular bacterial infection
    Herve Agaisse
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 309:1248-51. 2005
    ..monocytogenes...
  34. ncbi request reprint The Wingless morphogen gradient is established by the cooperative action of Frizzled and Heparan Sulfate Proteoglycan receptors
    Gyeong Hun Baeg
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Dev Biol 276:89-100. 2004
    ..Taken together, our analysis illustrates how the coordinated activities of HSPGs and Fz/DFz2 shape the Wg morphogen gradient...
  35. ncbi request reprint Signaling role of hemocytes in Drosophila JAK/STAT-dependent response to septic injury
    Herve Agaisse
    Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Dev Cell 5:441-50. 2003
    ....
  36. ncbi request reprint Genome-wide RNAi analysis of growth and viability in Drosophila cells
    Michael Boutros
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 303:832-5. 2004
    ..Such a systematic screen for cell phenotypes, such as cell viability, can thus be effective in characterizing functionally related genes on a genome-wide scale...
  37. pmc Inactivation of Drosophila Huntingtin affects long-term adult functioning and the pathogenesis of a Huntington's disease model
    Sheng Zhang
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Dis Model Mech 2:247-66. 2009
    ....
  38. ncbi request reprint Drosophila Wnt/Fz pathways
    Ramanuj DasGupta
    Department of Genetics, Harvard Medical School HHMI, Boston, MA 02115, USA
    Sci STKE 2005:cm5. 2005
    ..Upon Wnt/Wg stimulation, stabilized Arm translocates to the nucleus, where, together with LEF/TCF transcription factors, it activates downstream target genes that regulate numerous cell biological processes...
  39. ncbi request reprint Yantar, a conserved arginine-rich protein is involved in Drosophila hemocyte development
    Sergey A Sinenko
    Division of Pediatric Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Dev Biol 273:48-62. 2004
    ..Ytr function has been preserved in evolution as hematopoietic specific expression of the Drosophila or mouse Ytr proteins rescue the differentiation defects in mutant hemocytes...
  40. ncbi request reprint Extrusion and death of DPP/BMP-compromised epithelial cells in the developing Drosophila wing
    Matthew C Gibson
    Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Science 307:1785-9. 2005
    ..We propose that in addition to assigning cell fates, a crucial developmental function of DPP/BMP signaling is the position-specific control of epithelial architecture...
  41. pmc The Hippo signaling pathway interactome
    Young Kwon
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 342:737-40. 2013
    ..Given the importance of the Hippo pathway in tumor development, the Hippo-PPIN will contribute to our understanding of this network in both normal growth and cancer. ..
  42. pmc FOXO/4E-BP signaling in Drosophila muscles regulates organism-wide proteostasis during aging
    Fabio Demontis
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Cell 143:813-25. 2010
    ..These findings reveal an organism-wide regulation of proteostasis in response to muscle aging and a key role of FOXO/4E-BP signaling in the coordination of organismal and tissue aging...
  43. pmc ESCRT factors restrict mycobacterial growth
    Jennifer A Philips
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, 77 Louis Pasteur Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:3070-5. 2008
    ..Furthermore, we demonstrate that, in mammalian cells, the ESCRT machinery plays a conserved role in restricting bacterial growth...
  44. pmc Comparative analysis of argonaute-dependent small RNA pathways in Drosophila
    Rui Zhou
    Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, Boston, MA 02115, USA
    Mol Cell 32:592-9. 2008
    ..Our studies indicate that many components are shared among all three Argonaute-dependent silencing pathways, though each is also impacted by discrete sets of genes...
  45. ncbi request reprint The promise and perils of Wnt signaling through beta-catenin
    Randall T Moon
    Howard Hughes Medical Institute, Department of Pharmacology, and Center for Developmental Biology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Science 296:1644-6. 2002
    ..The STKE Connections Maps for these pathways provide an important tool in accessing this large body of complex information...
  46. pmc UP-TORR: online tool for accurate and Up-to-Date annotation of RNAi Reagents
    Yanhui Hu
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
    Genetics 195:37-45. 2013
    ..Thus, UP-TORR allows users to choose the most appropriate RNAi reagents at the onset of a study, as well as to perform the most appropriate analyses of results of RNAi-based studies. ..
  47. pmc FlyPrimerBank: an online database for Drosophila melanogaster gene expression analysis and knockdown evaluation of RNAi reagents
    Yanhui Hu
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
    G3 (Bethesda) 3:1607-16. 2013
    ..e., to avoid amplification of the RNAi reagent itself). We demonstrate the utility of this resource for validation of RNAi reagents in vivo. ..
  48. pmc Phosphorylation networks regulating JNK activity in diverse genetic backgrounds
    Chris Bakal
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02215, USA
    Science 322:453-6. 2008
    ....
  49. ncbi request reprint Integrated activity of PDZ protein complexes regulates epithelial polarity
    David Bilder
    Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Nat Cell Biol 5:53-8. 2003
    ..Our data suggest a model in which the maturation of epithelial cell polarity is driven by integration of the sequential activities of PDZ-based protein complexes...
  50. pmc Processing of Drosophila endo-siRNAs depends on a specific Loquacious isoform
    Rui Zhou
    Harvard Medical School, Department of Genetics, Howard Hughes Medical Institute, Boston, Massachusetts 02115, USA
    RNA 15:1886-95. 2009
    ....
  51. ncbi request reprint Design and implementation of high-throughput RNAi screens in cultured Drosophila cells
    Nadire Ramadan
    Department of Genetics and Drosophila RNAi Screening Center, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Protoc 2:2245-64. 2007
    ..Depending on the scope of the screen and subsequent analysis and validation involved, the full protocol can take anywhere from 3 months to 2 years to complete...
  52. ncbi request reprint Apicobasal polarization: epithelial form and function
    Matthew C Gibson
    Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston MA 02115, USA
    Curr Opin Cell Biol 15:747-52. 2003
    ..Recent findings show that segregation of ligand and receptor with one on each side of this barrier can be a crucial regulator of cell-cell signaling events...
  53. pmc Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila
    Gyeong Hun Baeg
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Genes Dev 19:1861-70. 2005
    ..Our study has uncovered many uncharacterized genes required for different steps of the JAK/STAT signaling pathway...
  54. pmc Use of a label-free quantitative platform based on MS/MS average TIC to calculate dynamics of protein complexes in insulin signaling
    Xuemei Yang
    Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    J Biomol Tech 20:272-7. 2009
    ..The MS strategy showed excellent coverage of known components in addition to potentially novel interactions...
  55. pmc Embryonic multipotent progenitors remodel the Drosophila airways during metamorphosis
    Chrysoula Pitsouli
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Development 137:3615-24. 2010
    ..Our study establishes the series of events that lead to the formation of an adult tubular structure in Drosophila...
  56. ncbi request reprint The PDGF/VEGF receptor controls blood cell survival in Drosophila
    Katja Brückner
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Dev Cell 7:73-84. 2004
    ..This function of PVR shows striking conservation with mammalian hematopoiesis and establishes Drosophila as a model to study hematopoietic cell survival in development and disease...
  57. ncbi request reprint Roles of myosin phosphatase during Drosophila development
    Change Tan
    Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Development 130:671-81. 2003
    ..Our results indicate that the regulation of the phosphorylation of myosin regulatory light chains, or dynamic activation and inactivation of myosin II, is essential for its various functions during many developmental processes...
  58. pmc Integration of Insulin receptor/Foxo signaling and dMyc activity during muscle growth regulates body size in Drosophila
    Fabio Demontis
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Development 136:983-93. 2009
    ..Thus, maximal dMyc transcriptional activity depends on InR to control muscle mass, which in turn induces a systemic behavioral response to allocate body size and proportions...
  59. ncbi request reprint Cellular phenotype recognition for high-content RNA interference genome-wide screening
    Jun Wang
    Center for Bioinformatics, Harvard Center for Neurodegeneration and Repair, Harvard Medical School, Boston, Massachusetts, USA
    J Biomol Screen 13:29-39. 2008
    ..Moreover, the authors applied this approach to analyze the whole high-content fluorescence images of Drosophila cells for further HCS-based gene function analysis...
  60. pmc A genomewide RNA interference screen for modifiers of aggregates formation by mutant Huntingtin in Drosophila
    Sheng Zhang
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genetics 184:1165-79. 2010
    ..Thus, other aggregates regulators isolated in our screen may identify additional genes involved in the protein-folding pathway and neurotoxicity...
  61. pmc A genome-wide RNA interference screen identifies putative chromatin regulators essential for E2F repression
    Jianrong Lu
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:9381-6. 2007
    ..These findings suggest that potential chromatin regulation mediated by Domino and PcG-like factors plays an important role in controlling E2F activity and cell growth...
  62. ncbi request reprint Evidence that stem cells reside in the adult Drosophila midgut epithelium
    Craig A Micchelli
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nature 439:475-9. 2006
    ..The ability to identify, manipulate and genetically trace cell lineages in the midgut should lead to the discovery of additional genes that regulate stem and progenitor cell biology in the gastrointestinal tract...
  63. ncbi request reprint A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling
    Adam Friedman
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nature 444:230-4. 2006
    ..Novel regulators that modulate specific pathway outputs may be selective targets for drug discovery...
  64. ncbi request reprint A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway
    Kent Nybakken
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nat Genet 37:1323-32. 2005
    ..Finally, we showed that several of these genes have conserved roles in mammalian Hh signaling...
  65. pmc Activation of the JNK pathway during dorsal closure in Drosophila requires the mixed lineage kinase, slipper
    Beth Stronach
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 16:377-87. 2002
    ..Furthermore, our results show that other putative JNKKKs cannot compensate for the loss of slpr function and, thus, may regulate other JNK or MAPK-dependent processes...
  66. pmc Ecdysone signaling at metamorphosis triggers apoptosis of Drosophila abdominal muscles
    JONATHAN ZIRIN
    Department of Genetics, Harvard Medical School, USA Electronic address
    Dev Biol 383:275-84. 2013
    ..Thus, there is no set rule as to the role of autophagy and apoptosis during Drosophila histolysis. ..
  67. pmc Depleting gene activities in early Drosophila embryos with the "maternal-Gal4-shRNA" system
    Max V Staller
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    Genetics 193:51-61. 2013
    ..We apply this powerful and simple method to unravel the embryonic functions of a number of pleiotropic genes...
  68. pmc Drosophila cytokine unpaired 2 regulates physiological homeostasis by remotely controlling insulin secretion
    Akhila Rajan
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Cell 151:123-37. 2012
    ..Strikingly, we find that human Leptin can rescue the upd2 mutant phenotypes, suggesting that Upd2 is the functional homolog of Leptin...
  69. pmc FlyRNAi: the Drosophila RNAi screening center database
    Ian Flockhart
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Nucleic Acids Res 34:D489-94. 2006
    ..Data from published screens are available to the public through a highly configurable interface that allows detailed examination of the data and provides access to a number of other databases and bioinformatics tools...
  70. pmc FlyRNAi.org--the database of the Drosophila RNAi screening center: 2012 update
    Ian T Flockhart
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Nucleic Acids Res 40:D715-9. 2012
    ..Overall, the result is a more useful, flexible and comprehensive website and database...
  71. ncbi request reprint High-throughput approaches to dissecting MAPK signaling pathways
    Adam Friedman
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Methods 40:262-71. 2006
    ..We also provide examples of secondary validation screens and methods for managing large datasets for future in vivo functional characterization...
  72. ncbi request reprint Temperature-sensitive control of protein activity by conditionally splicing inteins
    Martin P Zeidler
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nat Biotechnol 22:871-6. 2004
    ....
  73. ncbi request reprint SALS, a WH2-domain-containing protein, promotes sarcomeric actin filament elongation from pointed ends during Drosophila muscle growth
    Jianwu Bai
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Dev Cell 13:828-42. 2007
    ....
  74. pmc Identification of neural outgrowth genes using genome-wide RNAi
    Katharine J Sepp
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Genet 4:e1000111. 2008
    ..Collectively, our results showed that RNAi phenotypes in primary neural culture can parallel in vivo phenotypes, and the screening technique can be used to identify many new genes that have important functions in the nervous system...
  75. doi request reprint Culture of Drosophila primary cells dissociated from gastrula embryos and their use in RNAi screening
    Jianwu Bai
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
    Nat Protoc 4:1502-12. 2009
    ..The entire protocol takes approximately 14 d, whereas the preparation of primary cells from Drosophila embryos only requires 2-4 h...
  76. ncbi request reprint A Drosophila homolog of cyclase-associated proteins collaborates with the Abl tyrosine kinase to control midline axon pathfinding
    Zachary Wills
    Department of Cell Biology and Program in Neuroscience, Harvard Medical School, Boston, MA 02115, USA
    Neuron 36:611-22. 2002
    ..These studies identify Capulet as part of an emerging pathway linking guidance signals to regulation of cytoskeletal dynamics and suggest that the Abl pathway mediates signals downstream of multiple Roundabout receptors...
  77. ncbi request reprint High-throughput RNA interference screens in Drosophila tissue culture cells
    Susan Armknecht
    Department of Genetics, Harvard Medical School, Drosophila RNAi Screening Center, Boston, MA 02115, USA
    Methods Enzymol 392:55-73. 2005
    ....
  78. ncbi request reprint The emergence of geometric order in proliferating metazoan epithelia
    Matthew C Gibson
    Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 442:1038-41. 2006
    ....
  79. ncbi request reprint Gamma-secretase/presenilin inhibitors for Alzheimer's disease phenocopy Notch mutations in Drosophila
    Craig A Micchelli
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts, USA
    FASEB J 17:79-81. 2003
    ....
  80. ncbi request reprint Homeostasis in infected epithelia: stem cells take the lead
    Chrysoula Pitsouli
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Cell Host Microbe 6:301-7. 2009
    ..In this Short Review, we discuss recent developments in the exciting field of host epithelia-pathogen interaction in Drosophila as well as in mammals...
  81. ncbi request reprint Mechanism of inhibition of the Drosophila and mammalian EGF receptors by the transmembrane protein Kekkon 1
    Christian Ghiglione
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Development 130:4483-93. 2003
    ....
  82. ncbi request reprint Genetic screening for signal transduction in the era of network biology
    Adam Friedman
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02175, USA
    Cell 128:225-31. 2007
    ....
  83. ncbi request reprint Quantitative morphological signatures define local signaling networks regulating cell morphology
    Chris Bakal
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 316:1753-6. 2007
    ..We analyzed a compendium of quantitative morphological signatures and described the existence of local signaling networks that act to regulate cell protrusion, adhesion, and tension...
  84. pmc An endogenous small interfering RNA pathway in Drosophila
    Benjamin Czech
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 453:798-802. 2008
    ..These observations expand the repertoire of small RNAs in Drosophila, adding a class that blurs distinctions based on known biogenesis mechanisms and functional roles...
  85. pmc Differential requirement for STAT by gain-of-function and wild-type receptor tyrosine kinase Torso in Drosophila
    Willis X Li
    Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Development 129:4241-8. 2002
    ..Our findings indicate that the Ras/Raf/MEK/MAPK signaling pathway is sufficient to mediate the normal functions of wild-type RTK, whereas the effects of gain-of-function mutant RTK additionally require STAT activation...
  86. pmc Retraction of the Drosophila germ band requires cell-matrix interaction
    Frieder Schock
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 17:597-602. 2003
    ..We further implicate a role for this spreading in the process of germ-band retraction...
  87. pmc Parallel chemical genetic and genome-wide RNAi screens identify cytokinesis inhibitors and targets
    Ulrike S Eggert
    Department of Systems Biology, Harvard Medical School Boston, Massachusetts, USA
    PLoS Biol 2:e379. 2004
    ..Our study shows that parallel RNA interference and small molecule screening is a generally useful approach to identifying active small molecules and their target pathways...
  88. pmc Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition
    Sara Cherry
    Department of Genetics, Harvard Medical School and Howard Hughes Medical Instutite, Boston, Massachusetts 02115, USA
    Genes Dev 19:445-52. 2005
    ..Finally, this increased sensitivity to ribosome levels also holds true for poliovirus infection of human cells, demonstrating the generality of these findings...
  89. ncbi request reprint Mechanism of activation of the Drosophila EGF Receptor by the TGFalpha ligand Gurken during oogenesis
    Christian Ghiglione
    Department of Genetics, Medical Institute, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Development 129:175-86. 2002
    ..These two proteins collaborate to promote an activating proteolytic cleavage and release of Grk. After cleavage, the extracellular domain of Grk is secreted from the oocyte to activate the Egfr in the follicular epithelium...
  90. ncbi request reprint Entry is a rate-limiting step for viral infection in a Drosophila melanogaster model of pathogenesis
    Sara Cherry
    Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Immunol 5:81-7. 2004
    ..Thus, this virus model provides a sensitive and efficient approach for identifying components required for pathogenesis...
  91. ncbi request reprint Sequential activation of signaling pathways during innate immune responses in Drosophila
    Michael Boutros
    Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Dev Cell 3:711-22. 2002
    ..In particular, our results may imply a close link between the control of tissue repair and antimicrobial processes...
  92. pmc A sensitized genetic screen to identify novel regulators and components of the Drosophila janus kinase/signal transducer and activator of transcription pathway
    Erika A Bach
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genetics 165:1149-66. 2003
    ....
  93. ncbi request reprint In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography
    Claudio Vinegoni
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th Street, Charlestown, Massachusetts 02129, USA
    Nat Methods 5:45-7. 2008
    ..We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time...
  94. ncbi request reprint Molecular mechanisms of epithelial morphogenesis
    Frieder Schock
    Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Annu Rev Cell Dev Biol 18:463-93. 2002
    ..Our discussion is mostly focused on results from Drosophila and mammalian tissue culture but also draws on the insights gained from other organisms...
  95. pmc The twin spot generator for differential Drosophila lineage analysis
    Ruth Griffin
    Commissariat a l Energie Atomique, Institut de Recherches en Technologies et Sciences pour le Vivant, Centre National de la Recherche Scientifique, Grenoble, France
    Nat Methods 6:600-2. 2009
    ..We propose wide applications of TSG to lineage and genetic mosaic studies...
  96. pmc A genome-wide gene function prediction resource for Drosophila melanogaster
    Han Yan
    Department of Cancer Biology, Center for Cancer Systems Biology CCSB, Dana Farber Cancer Institute, Boston, Massachusetts, United States of America
    PLoS ONE 5:e12139. 2010
    ..The resulting resource of prioritized associations between Drosophila genes and their potential functions offers a guide for experimental investigations...
  97. pmc Slalom encodes an adenosine 3'-phosphate 5'-phosphosulfate transporter essential for development in Drosophila
    Florian Lüders
    Department of Cell and Molecular Biology, BMC B13, Lund University, 22184 Lund, Sweden
    EMBO J 22:3635-44. 2003
    ....
  98. pmc The fruitless gene is required for the proper formation of axonal tracts in the embryonic central nervous system of Drosophila
    Ho Juhn Song
    Department of Zoology, Oregon State University, Corvallis, Oregon 97331 2914, USA
    Genetics 162:1703-24. 2002
    ..Our results suggest that one of fru's sex-nonspecific roles is to regulate the pathfinding ability of axons in the embryonic CNS...
  99. ncbi request reprint The Jak/STAT pathway in model organisms: emerging roles in cell movement
    Steven X Hou
    The Laboratory of Immunobiology, National Cancer Institute at Frederick, Building 560, Room 12 70, MD 21702, USA
    Dev Cell 3:765-78. 2002
    ..In particular, we emphasize recent insights into the role that this pathway plays in the control of cell movement...

Research Grants36

  1. High-throughput screening assay development for axonopathic neurodegeration
    Norbert Perrimon; Fiscal Year: 2006
    ..The long term goal of this work is thus to understand better the causes of axonal destruction in neurodegenerative diseases, and to find drugs that can slow or prevent axonal degeneration from occurring. ..
  2. Characterization of the Insulin to Autophagy Pathway in Muscles
    Norbert Perrimon; Fiscal Year: 2010
    ....
  3. ROLES OF HEPARAN SULFATE PROTEOGLYCANS IN SIGNALING
    Norbert Perrimon; Fiscal Year: 2000
    ..Because these pathways have also been implicated in various disease stages; e.g., oncogenesis, our studies may lead to novel ways to modulate specifically the activity of these signaling pathways. ..
  4. Viral Immunity in Drosophila
    Norbert Perrimon; Fiscal Year: 2007
    ..Because our insect virus system is sensitive to drugs that are used against human viruses, our studies may lead to the identification of novel antiviral compounds that may prove effective in mammalian cells. ..
  5. Functional Genomic analysis using RNAi screen in Drosophila
    Norbert Perrimon; Fiscal Year: 2007
    ....
  6. Network Topology in Cell Signaling
    Norbert Perrimon; Fiscal Year: 2007
    ..Altogether, these studies will greatly advance our knowledge of signaling networks and their organization during signal transduction. [unreadable] [unreadable] [unreadable]..
  7. Host factors involved in bacterial pathogenesis
    Norbert Perrimon; Fiscal Year: 2008
    ..tuberculosis and its human host. ..
  8. Network Topology in Cell Signaling
    Norbert Perrimon; Fiscal Year: 2008
    ..Altogether, these studies will greatly advance our knowledge of signaling networks and their organization during signal transduction. [unreadable] [unreadable] [unreadable]..
  9. Functional Genomic analysis using RNAi screen in Drosophila
    Norbert Perrimon; Fiscal Year: 2008
    ....
  10. Drosophila Transgenic RNAi Resource Project
    Norbert Perrimon; Fiscal Year: 2008
    ....
  11. Host factors involved in bacterial pathogenesis
    Norbert Perrimon; Fiscal Year: 2009
    ..tuberculosis and its human host. ..
  12. Functional Genomic analysis using RNAi screen in Drosophila
    Norbert Perrimon; Fiscal Year: 2009
    ....
  13. Drosophila Transgenic RNAi Resource Project
    Norbert Perrimon; Fiscal Year: 2009
    ....
  14. Functional Genomic analysis using RNAi screen in Drosophila
    Norbert Perrimon; Fiscal Year: 2010
    ....
  15. Network analysis of Signal Transduction
    Norbert Perrimon; Fiscal Year: 2010
    ..The success of this application will provide a benchmark for reconstructing signaling networks on a global scale. ..
  16. Drosophila Transgenic RNAi Resource Project
    Norbert Perrimon; Fiscal Year: 2010
    ....
  17. Functional Genomic analysis using RNAi screen in Drosophila
    Norbert Perrimon; Fiscal Year: 2009
    ....
  18. Host factors involved in bacterial pathogenesis
    Norbert Perrimon; Fiscal Year: 2007
    ..tuberculosis and its human host. [unreadable] [unreadable] [unreadable]..
  19. Functional Genomic Screen using RNAi in Drosophila
    Norbert Perrimon; Fiscal Year: 2005
    ..Finally, to demonstrate the value of the approach, we describe a number of cell-based screens that will identify genes involved in cell survival, cytokinesis, cell shape changes and immunity. ..
  20. ROLES OF HEPARAN SULFATE PROTEOGLYCANS IN SIGNALING
    Norbert Perrimon; Fiscal Year: 2001
    ..Because these pathways have also been implicated in various disease stages; e.g., oncogenesis, our studies may lead to novel ways to modulate specifically the activity of these signaling pathways. ..
  21. ROLES OF HEPARAN SULFATE PROTEOGLYCANS IN SIGNALING
    Norbert Perrimon; Fiscal Year: 2002
    ..Because these pathways have also been implicated in various disease stages; e.g., oncogenesis, our studies may lead to novel ways to modulate specifically the activity of these signaling pathways. ..
  22. Viral Immunity in Drosophila
    Norbert Perrimon; Fiscal Year: 2003
    ..Because our insect virus system is sensitive to drugs that are used against human viruses, our studies may lead to the identification of novel antiviral compounds that may prove effective in mammalian cells. ..
  23. Functional Genomic Screen using RNAi in Drosophila
    Norbert Perrimon; Fiscal Year: 2003
    ..Finally, to demonstrate the value of the approach, we describe a number of cell-based screens that will identify genes involved in cell survival, cytokinesis, cell shape changes and immunity. ..
  24. ROLES OF HEPARAN SULFATE PROTEOGLYCANS IN SIGNALING
    Norbert Perrimon; Fiscal Year: 2003
    ..Because these pathways have also been implicated in various disease stages; e.g., oncogenesis, our studies may lead to novel ways to modulate specifically the activity of these signaling pathways. ..
  25. Viral Immunity in Drosophila
    Norbert Perrimon; Fiscal Year: 2004
    ..Because our insect virus system is sensitive to drugs that are used against human viruses, our studies may lead to the identification of novel antiviral compounds that may prove effective in mammalian cells. ..
  26. Functional Genomic Screen using RNAi in Drosophila
    Norbert Perrimon; Fiscal Year: 2004
    ..Finally, to demonstrate the value of the approach, we describe a number of cell-based screens that will identify genes involved in cell survival, cytokinesis, cell shape changes and immunity. ..
  27. Viral Immunity in Drosophila
    Norbert Perrimon; Fiscal Year: 2005
    ..Because our insect virus system is sensitive to drugs that are used against human viruses, our studies may lead to the identification of novel antiviral compounds that may prove effective in mammalian cells. ..
  28. High-throughput screen:Listeria infection (RMI)
    Norbert Perrimon; Fiscal Year: 2004
    ....
  29. Functional Genomic Screen using RNAi in Drosophila
    Norbert Perrimon; Fiscal Year: 2005
    ..Finally, to demonstrate the value of the approach, we describe a number of cell-based screens that will identify genes involved in cell survival, cytokinesis, cell shape changes and immunity. ..
  30. Conference on Cell Polarity & Asymmetric Cell Divisions
    Norbert Perrimon; Fiscal Year: 2004
    ..The goal of the meeting is to bring together the leading experts that study the mechanism of cell polarization and cell asymmetry to discuss emerging concepts. ..
  31. Viral Immunity in Drosophila
    Norbert Perrimon; Fiscal Year: 2006
    ..Because our insect virus system is sensitive to drugs that are used against human viruses, our studies may lead to the identification of novel antiviral compounds that may prove effective in mammalian cells. ..
  32. Functional Genomic Screen using RNAi in Drosophila
    Norbert Perrimon; Fiscal Year: 2006
    ..Finally, to demonstrate the value of the approach, we describe a number of cell-based screens that will identify genes involved in cell survival, cytokinesis, cell shape changes and immunity. ..
  33. Host factors involved in bacterial pathogenesis
    Norbert Perrimon; Fiscal Year: 2006
    ..tuberculosis and its human host. [unreadable] [unreadable] [unreadable]..
  34. New collections of dsRNAs and overexpression miRNA constructs to supplement RNAi
    Norbert Perrimon; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  35. Drosophila Transgenic RNAi Resource Project
    Norbert Perrimon; Fiscal Year: 2009
    ....