Joao A Paulo

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Post-translational modifications of pancreatic fluid proteins collected via the endoscopic pancreatic function test (ePFT)
    Joao A Paulo
    Department of Pathology, Children s Hospital Boston, Boston, MA, United States Proteomics Center at Children s Hospital Boston, Boston, MA, United States Center for Pancreatic Disease, Division of Gastroenterology, Hepatology and Endoscopy, Brigham and Women s Hospital and Department of Medicine, Harvard Medical School, Boston, MA, United States Electronic address
    J Proteomics 92:216-27. 2013
  2. doi request reprint Mass spectrometry-based quantitative proteomic profiling of human pancreatic and hepatic stellate cell lines
    Joao A Paulo
    Department of Pathology, Boston Children s Hospital, Boston, MA 02115, USA
    Genomics Proteomics Bioinformatics 11:105-13. 2013
  3. doi request reprint Analysis of endoscopic pancreatic function test (ePFT)-collected pancreatic fluid proteins precipitated via ultracentrifugation
    Joao A Paulo
    Department of Pathology, Children s Hospital Boston and Harvard Medical School, Boston, MA 02115, USA
    JOP 14:176-86. 2013
  4. pmc Subcellular fractionation enhances proteome coverage of pancreatic duct cells
    Joao A Paulo
    Department of Pathology, Children s Hospital Boston, Boston, MA, USA
    Biochim Biophys Acta 1834:791-7. 2013
  5. ncbi request reprint A proteomic comparison of formalin-fixed paraffin-embedded pancreatic tissue from autoimmune pancreatitis, chronic pancreatitis, and pancreatic cancer
    Joao A Paulo
    Department of Pathology, Brigham and Women s Hospital and Department of Medicine, Harvard Medical School Boston, MA 02115, USA
    JOP 14:405-14. 2013
  6. pmc Identification of pancreas-specific proteins in endoscopically (endoscopic pancreatic function test) collected pancreatic fluid with liquid chromatography--tandem mass spectrometry
    Joao A Paulo
    Center for Pancreatic Disease, Division of Gastroenterology, Hepatology, and Endoscopy, Brigham and Women s Hospital, 75 Francis St, Boston, MA 02115, USA
    Pancreas 39:889-96. 2010
  7. pmc Proteomic analysis (GeLC-MS/MS) of ePFT-collected pancreatic fluid in chronic pancreatitis
    Joao A Paulo
    Department of Pathology, Children s Hospital Boston, Boston, Massachusettes, United States
    J Proteome Res 11:1897-912. 2012
  8. pmc Proteomic analysis of formalin-fixed paraffin-embedded pancreatic tissue using liquid chromatography tandem mass spectrometry
    Joao A Paulo
    Department of Pathology, Harvard Medical School, Boston, MA, USA
    Pancreas 41:175-85. 2012
  9. pmc Optimized sample preparation of endoscopic collected pancreatic fluid for SDS-PAGE analysis
    Joao A Paulo
    Department of Pathology, Children s Hospital Boston and Harvard Medical School, Boston, MA 02115, USA
    Electrophoresis 31:2377-87. 2010
  10. pmc Mass spectrometry-based proteomics of endoscopically collected pancreatic fluid in chronic pancreatitis research
    Joao A Paulo
    Center for Pancreatic Disease, Division of Gastroenterology, Hepatology and Endoscopy, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    Proteomics Clin Appl 5:109-20. 2011

Detail Information

Publications23

  1. pmc Post-translational modifications of pancreatic fluid proteins collected via the endoscopic pancreatic function test (ePFT)
    Joao A Paulo
    Department of Pathology, Children s Hospital Boston, Boston, MA, United States Proteomics Center at Children s Hospital Boston, Boston, MA, United States Center for Pancreatic Disease, Division of Gastroenterology, Hepatology and Endoscopy, Brigham and Women s Hospital and Department of Medicine, Harvard Medical School, Boston, MA, United States Electronic address
    J Proteomics 92:216-27. 2013
    ..We aimed to identify differences in posttranslational modifications (PTMs) in pancreatic fluid proteins from individuals with chronic pancreatitis (n=9) and non-pancreatitis controls (n=9)...
  2. doi request reprint Mass spectrometry-based quantitative proteomic profiling of human pancreatic and hepatic stellate cell lines
    Joao A Paulo
    Department of Pathology, Boston Children s Hospital, Boston, MA 02115, USA
    Genomics Proteomics Bioinformatics 11:105-13. 2013
    ....
  3. doi request reprint Analysis of endoscopic pancreatic function test (ePFT)-collected pancreatic fluid proteins precipitated via ultracentrifugation
    Joao A Paulo
    Department of Pathology, Children s Hospital Boston and Harvard Medical School, Boston, MA 02115, USA
    JOP 14:176-86. 2013
    ..We have shown previously that trichloroacetic acid precipitation is an effective method of protein extraction from pancreatic fluid for downstream biomarker discovery, compared to other common extraction methods tested...
  4. pmc Subcellular fractionation enhances proteome coverage of pancreatic duct cells
    Joao A Paulo
    Department of Pathology, Children s Hospital Boston, Boston, MA, USA
    Biochim Biophys Acta 1834:791-7. 2013
    ....
  5. ncbi request reprint A proteomic comparison of formalin-fixed paraffin-embedded pancreatic tissue from autoimmune pancreatitis, chronic pancreatitis, and pancreatic cancer
    Joao A Paulo
    Department of Pathology, Brigham and Women s Hospital and Department of Medicine, Harvard Medical School Boston, MA 02115, USA
    JOP 14:405-14. 2013
    ....
  6. pmc Identification of pancreas-specific proteins in endoscopically (endoscopic pancreatic function test) collected pancreatic fluid with liquid chromatography--tandem mass spectrometry
    Joao A Paulo
    Center for Pancreatic Disease, Division of Gastroenterology, Hepatology, and Endoscopy, Brigham and Women s Hospital, 75 Francis St, Boston, MA 02115, USA
    Pancreas 39:889-96. 2010
    ....
  7. pmc Proteomic analysis (GeLC-MS/MS) of ePFT-collected pancreatic fluid in chronic pancreatitis
    Joao A Paulo
    Department of Pathology, Children s Hospital Boston, Boston, Massachusettes, United States
    J Proteome Res 11:1897-912. 2012
    ..Our workflow provided a mass spectrometry-based approach for the further study of the pancreatic fluid proteome, which may lead to the discovery potential biomarkers of chronic pancreatitis...
  8. pmc Proteomic analysis of formalin-fixed paraffin-embedded pancreatic tissue using liquid chromatography tandem mass spectrometry
    Joao A Paulo
    Department of Pathology, Harvard Medical School, Boston, MA, USA
    Pancreas 41:175-85. 2012
    ..We aim to establish liquid chromatography coupled with tandem mass spectrometry analysis of FFPE pancreatic tissue as a suitable strategy for the study of the pancreas proteome...
  9. pmc Optimized sample preparation of endoscopic collected pancreatic fluid for SDS-PAGE analysis
    Joao A Paulo
    Department of Pathology, Children s Hospital Boston and Harvard Medical School, Boston, MA 02115, USA
    Electrophoresis 31:2377-87. 2010
    ..We have also determined the addition of protease inhibitors to be assay-dependent. Our optimized sample preparation strategy can be applied to future proteomic analyses of pancreatic fluid...
  10. pmc Mass spectrometry-based proteomics of endoscopically collected pancreatic fluid in chronic pancreatitis research
    Joao A Paulo
    Center for Pancreatic Disease, Division of Gastroenterology, Hepatology and Endoscopy, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    Proteomics Clin Appl 5:109-20. 2011
    ..We conclude that pancreatic fluid represents a rich reservoir of potential biomarkers and that the study of the molecular mechanisms of chronic pancreatitis may benefit substantially from MS-based proteomics...
  11. pmc Difference gel electrophoresis identifies differentially expressed proteins in endoscopically collected pancreatic fluid
    Joao A Paulo
    Department of Pathology, Children s Hospital Boston and Harvard Medical School, Boston, MA 02115, USA
    Electrophoresis 32:1939-51. 2011
    ..Endoscopic collection (ePFT) in tandem with DIGE-LC-MS/MS is a suitable approach for pancreatic fluid proteome analysis; however, further optimization of our protocol, as outlined herein, may improve proteome coverage in future analyses...
  12. doi request reprint Cigarette smoking impairs pancreatic duct cell bicarbonate secretion
    Vivek Kadiyala
    Center for Pancreatic Disease, Division of Gastroenterology, Hepatology and Endoscopy, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    JOP 14:31-8. 2013
    ..To compare pancreatic duct cell function in smokers (current and past) and never smokers by measurement of secretin-stimulated peak bicarbonate concentration ([HCO3-]) in endoscopic collected pancreatic fluid (PF)...
  13. doi request reprint Inflammatory protein profiling of pancreatic cyst fluid using EUS-FNA in tandem with cytokine microarray differentiates between branch duct IPMN and inflammatory cysts
    Linda S Lee
    Center for Pancreatic Disease, Division of Gastroenterology Hepatology and Endoscopy, Brigham and Women s Hospital and Department of Medicine, Harvard Medical School, Boston, MA 02115, United States
    J Immunol Methods 382:142-9. 2012
    ..Statistical analysis was performed using non-parametric methods...
  14. pmc Cytokine profiling of pancreatic fluid using the ePFT collection method in tandem with a multiplexed microarray assay
    Joao A Paulo
    Department of Pathology, Children s Hospital Boston, Boston, MA, United States
    J Immunol Methods 369:98-107. 2011
    ..Further targeted investigation of cytokines in ePFT-collected fluid will broaden our knowledge of pancreatic immune response and pathogenesis in chronic pancreatitis...
  15. pmc Differentiating Branch Duct and Mixed IPMN in Endoscopically Collected Pancreatic Cyst Fluid via Cytokine Analysis
    Linda S Lee
    Center for Pancreatic Disease and Division of Gastroenterology, Hepatology and Endoscopy, Brigham and Women s Hospital and Department of Medicine, Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA
    Gastroenterol Res Pract 2012:247309. 2012
    ..05) was present in higher concentrations in mixed IPMNs. Conclusion. TGF-β1 and G-CSF detected in endoscopically collected pancreatic cyst fluid are potential diagnostic biomarkers capable of distinguishing mixed IPMN from BD-IPMN...
  16. ncbi request reprint Cross-species analysis of nicotine-induced proteomic alterations in pancreatic cells
    Joao A Paulo
    Department of Pathology, Children s Hospital Boston, Boston, MA 02115, USA
    Proteomics 13:1499-512. 2013
    ..Further interrogation of such pathways will lead to insights into the potential effect of nicotine on pancreatic cells at the biomolecular level and the extension of this concept to the effect of nicotine on pancreatic disease...
  17. pmc Proteomic analysis of a rat pancreatic stellate cell line using liquid chromatography tandem mass spectrometry (LC-MS/MS)
    Joao A Paulo
    Department of Pathology, Children s Hospital Boston, Boston, MA 02115, USA
    J Proteomics 75:708-17. 2011
    ....
  18. pmc Proteomic analysis of an immortalized mouse pancreatic stellate cell line identifies differentially-expressed proteins in activated vs nonproliferating cell states
    Joao A Paulo
    Center for Pancreatic Disease, Division of Gastroenterology, Hepatology and Endoscopy, Brigham and Women s Hospital and Department of Medicine, Harvard Medical School, Boston, Massachusettes, United States
    J Proteome Res 10:4835-44. 2011
    ..Investigation of the role of PaSC in the pathogenesis of chronic pancreatitis using the mass spectrometry-based proteomics strategy described herein will lead to further insights into the molecular mechanisms associated with the disease...
  19. pmc Proteomic analysis of endoscopically (endoscopic pancreatic function test) collected gastroduodenal fluid using in-gel tryptic digestion followed by LC-MS/MS
    Joao A Paulo
    Department of Pathology, Children s Hospital Boston, Boston, MA, USA
    Proteomics Clin Appl 4:715-25. 2010
    ..We use in-gel tryptic digestion followed by LC-MS/MS (GeLC-MS/MS) to profile the proteome of gastroduodenal fluid collected during the endoscopic pancreatic function test (ePFT)...
  20. pmc Mass spectrometry-based proteomics for translational research: a technical overview
    Joao A Paulo
    Center for Pancreatic Disease, Division of Gastroenterology, Hepatology and Endoscopy, Brigham and Women s Hospital and Department of Medicine, Harvard Medical School, Boston, MA, USA
    Yale J Biol Med 85:59-73. 2012
    ..Mass spectrometry-based proteome elucidation can reveal potential biomarkers and aid in the development of hypotheses for downstream investigation of the molecular mechanisms of disease...
  21. pmc Mass Spectrometry-Based (GeLC-MS/MS) Comparative Proteomic Analysis of Endoscopically (ePFT) Collected Pancreatic and Gastroduodenal Fluids
    Joao A Paulo
    1 Department of Pathology, Children s Hospital Boston and Harvard Medical School, Boston, Massachusetts, USA 2 Proteomics Center at Children s Hospital Boston, Boston, Massachusetts, USA 3 Center for Pancreatic Disease, Division of Gastroenterology, Hepatology and Endoscopy, Brigham and Women s Hospital and Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA
    Clin Transl Gastroenterol 3:e14. 2012
    ..We test the hypothesis that these endoscopically collected fluids have different proteomes. As such, we aim to show that the ePFT method can be used to collect fluid enriched in pancreatic proteins to test for pancreatic function...
  22. pmc Urine proteomics for discovery of improved diagnostic markers of Kawasaki disease
    Alex Kentsis
    Division of Hematology Oncology, Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston Children s Hospital, Harvard Medical School, Boston, MA, USA
    EMBO Mol Med 5:210-20. 2013
    ....
  23. pmc Brainstem deficiency of the 14-3-3 regulator of serotonin synthesis: a proteomics analysis in the sudden infant death syndrome
    Kevin G Broadbelt
    Department of Pathology, Children s Hospital Boston and Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Proteomics 11:M111.009530. 2012
    ..These data suggest a potential molecular defect in SIDS related to TPH2 regulation, as 14-3-3 is critical in this process...