Research Topics
| Vasilis NtziachristosSummaryAffiliation: Harvard University Country: USA Publications
Research Grants
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Detail Information
Publications
Probing physiology and molecular function using optical imaging: applications to breast cancerV Ntziachristos
Department of Bioengineering, University of Pennsylvania, Philadelphia, USA
Breast Cancer Res 3:41-6. 2001..These features could enhance diagnostic accuracy, lower the current state-of-the-art detection limits, and play a vital role in therapeutic strategy and monitoring...
Looking and listening to light: the evolution of whole-body photonic imagingVasilis Ntziachristos
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School CNY149, 13th Street 5406, Charlestown, Massachusetts 02129, USA
Nat Biotechnol 23:313-20. 2005..For photonic imaging to fully realize its potential, however, further progress will be needed in refining optical inversion methods and data acquisition techniques...
Visualization of antitumor treatment by means of fluorescence molecular tomography with an annexin V-Cy5.5 conjugateVasilis Ntziachristos
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 101:12294-9. 2004..Tomographic approaches are found essential for small-animal optical imaging and are potentially well suited for clinical drug development and monitoring...- Fluorescence imaging with near-infrared light: new technological advances that enable in vivo molecular imagingVasilis Ntziachristos
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th Street 5406, Charlestown MA 02129 2060, USA
Eur Radiol 13:195-208. 2003..We describe theoretical specifics, and we provide insight into its in vivo capacity and the sensitivity achieved. Finally, we discuss its clinical feasibility... - In vivo tomographic imaging of near-infrared fluorescent probesVasilis Ntziachristos
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Bldg 149 13th Street 5406, Charlestown, MA 02129 2060, USA
Mol Imaging 1:82-8. 2002..This validation of FMT with FRI demonstrated the spatial congruence of fluorochrome activation as determined by the two techniques... - Planar fluorescence imaging using normalized dataVasilis Ntziachristos
Massachusetts General Hospital and Harvard Medical School, Center for Molecular Imaging Research, Laboratory for Biooptics and Molecular Imaging, Charlestown, Massachusetts 02129, USA
J Biomed Opt 10:064007. 2005..Normalized planar imaging retains implementation simplicity and could be used to improve on standard fluorescence reflectance imaging and as a simplified alternative to the more integrated and accurate tomographic methods... - Fluorescence molecular tomography resolves protease activity in vivoVasilis Ntziachristos
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts, USA
Nat Med 8:757-60. 2002.... - MRI-guided diffuse optical spectroscopy of malignant and benign breast lesionsVasilis Ntziachristos
Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104 6059, USA
Neoplasia 4:347-54. 2002..It is foreseen that the technique can play a major role in functional activation studies of brain and muscle as well... - Fluorescence molecular imagingVasilis Ntziachristos
Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
Annu Rev Biomed Eng 8:1-33. 2006..Focus is given to small-animal imaging. However, while a broad spectrum of fluorescence reporter technologies and imaging methods are outlined, as necessary for biomedical research, and clinical translation as well... - Charge-coupled-device based scanner for tomography of fluorescent near-infrared probes in turbid mediaVasilis Ntziachristos
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown 02129, USA
Med Phys 29:803-9. 2002..These measurements delineate detection and reconstruction characteristics associated with imaging of novel classes of fluorescent probes developed for in vivo molecular and functional probing of tissues... - Diffuse optical tomography of highly heterogeneous mediaV Ntziachristos
Department of Bioengineering, University of Pennsylvania, Philadelphia 19104, USA
IEEE Trans Med Imaging 20:470-8. 2001..To eliminate the artifacts and improve the overall image reconstruction, we apply a data-correction algorithm that yields superior reconstruction results and is virtually independent of the degree of the background heterogeneity... - Early photon tomography allows fluorescence detection of lung carcinomas and disease progression in mice in vivoMark J Niedre
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
Proc Natl Acad Sci U S A 105:19126-31. 2008..The imaging fidelity demonstrated underscores a method that can use a wide range of fluorescent probes to accurately visualize cellular- and molecular-level events in whole animals in vivo... - Quantitative analysis of chemotherapeutic effects in tumors using in vivo staining and correlative histologyHeung Kook Choi
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
Cell Oncol 27:183-90. 2005..To microscopically analyze the chemotherapeutic response of tumors using in vivo staining based on an annexinV-Cy5.5 probe and independently asses their apoptotic count using quantitative histological analysis... - Mapping molecular agents distributions in whole mice hearts using born-normalized optical projection tomographyClaudio Vinegoni
Center for System Biology, Massachusetts General Hospital and Harvard Medical School, Richard B Simches Research Center, Boston, Massachusetts, United States of America
PLoS ONE 7:e34427. 2012..We observed extensive monocyte recruitment within and around the infarcted areas and discovered that monocytes were also extensively recruited into non-ischemic myocardium, beyond that of injured tissue, such as the septum... - In-vivo lung cancer imaging in mice using 360 degrees free-space fluorescence molecular tomographyNikolaos Deliolanis
Laboratory for Bio-optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown 02129, USA
Conf Proc IEEE Eng Med Biol Soc 1:2370-2. 2006..Experimental data on imaging lung cancer are presented. Overall, this imaging approach can offer unprecedented imaging performance in fluorescence molecular tomography of small animals... - Tomographic fluorescence mapping of tumor targetsXavier Montet
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
Cancer Res 65:6330-6. 2005..FMT measurements can be done serially, with short imaging times and within the same live animal. The described method should be valuable for rapidly profiling biological phenomena in vivo... - Transillumination fluorescence imaging in mice using biocompatible upconverting nanoparticlesClaudio Vinegoni
Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, 185 Cambridge Street, Boston, Massachusetts 02114, USA
Opt Lett 34:2566-8. 2009..The method holds great promise for artifact-free whole-body visualization of optical molecular probes... - A statistical approach to inverting the Born ratioDamon Hyde
Electrical and Computer Engineering Department, Northeastern University, Boston, MA 02115, USA
IEEE Trans Med Imaging 26:893-905. 2007..Results are presented for three experimental scenarios: phantom data with a homogeneous background, phantoms implanted within a small animal, and in vivo data using an exogenous probe... - Optical imaging of apoptosis as a biomarker of tumor response to chemotherapyEyk A Schellenberger
Center for Molecular Imaging Research, Massachusetts General Hospital, Charlestown, MA 02129, USA
Neoplasia 5:187-92. 2003..The method maybe used to image pharmacologic responses in other animal models and, potentially, may permit the clinical imaging of apoptosis with noninvasive or minimally invasive instrumentation... - Hybrid photoacoustic fluorescence molecular tomography using finite-element-based inversionDaniel Razansky
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA
Med Phys 34:4293-301. 2007..The potential promise of the suggested method is experimentally verified on tissue-mimicking fluorescent phantoms, where improvements in the quality of FMT reconstructions are observed when imaging at the presence of a large absorber... 
Surface Reconstruction for free-space 360 degrees fluorescence molecular tomography and the effects of animal motionTobias Lasser
Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hosiptal and Harvard Medical School, Charlestown, MA 02129, USA
IEEE Trans Med Imaging 27:188-94. 2008..We then employ this methodology to characterize the animal movement of anaesthetized animals. We find that the effects of animal movement on the FMT reconstructed image were within system resolution limits (approximately 0.07 cm)...- Volumetric tomography of fluorescent proteins through small animals in vivoGiannis Zacharakis
Laboratory for Bio-optics and Molecular Imaging, Center for Molecular Imaging Research, Harvard Medical School, Charlestown, MA 02129, USA
Proc Natl Acad Sci U S A 102:18252-7. 2005..This technology can significantly improve imaging capacity over the current state of the art and should find wide in vivo imaging applications in drug discovery, immunology, and cancer research... - Indocyanine green enables near-infrared fluorescence imaging of lipid-rich, inflamed atherosclerotic plaquesClaudio Vinegoni
Center for Molecular Imaging Research, Massachusetts General Hospital, Boston, MA 02114, USA
Sci Transl Med 3:84ra45. 2011..The atheroma-targeting capability of ICG has the potential to accelerate the clinical development of NIRF molecular imaging of high-risk plaques in humans... - Data specific spatially varying regularization for multimodal fluorescence molecular tomographyDamon Hyde
Computational Radiology Laboratory, Children s Hospital Boston, Harvard Medical School, Boston, MA 02115 USA
IEEE Trans Med Imaging 29:365-74. 2010..Results are presented for both simulated and experimental data sets, and show significant improvements in image quality as compared to traditional regularization techniques... - Inversion with early photonsGordon M Turner
Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, CNY 149 13th Street 5406, Charlestown, Massachusetts 02129, USA
Med Phys 34:1405-11. 2007..We find that all methods perform adequately given appropriate regularization parameters, and that an experimentally measured photon weight function yields superior results over two approximate weights that have been previously used... - Normalized Born ratio for fluorescence optical projection tomographyClaudio Vinegoni
Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
Opt Lett 34:319-21. 2009..We use the algorithm to three-dimensionally reconstruct and characterize a fluorescein isothiocyanate containing absorptive phantom and an infarcted mouse heart previously injected with a fluorescent molecular probe... - Visualization of pulmonary inflammation using noninvasive fluorescence molecular imagingJodi Haller
Center for Molecular Imaging Research Laboratory For Bio optics and Molecular Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, USA
J Appl Physiol 104:795-802. 2008..The data presented herein serve to further evince fluorescence molecular imaging as a technology highly appropriate for the biomedical laboratory... - Normalized transillumination of fluorescent proteins in small animalsGiannis Zacharakis
Harvard Medical School, MA, USA
Mol Imaging 5:153-9. 2006..Due to the balance achieved between simplicity and accuracy, normalized transillumination approaches could serve as an important alternative molecular imaging method... - Comparison of fluorescence tomographic imaging in mice with early-arriving and quasi-continuous-wave photonsMark Niedre
Department of Electrical and Computer Engineering, Northeastern University, Boston, Massachusetts 02115, USA
Opt Lett 35:369-71. 2010.... - Tomographic fluorescence imaging of tumor vascular volume in miceXavier Montet
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th St, Room 5403, Charlestown, MA 02129, USA
Radiology 242:751-8. 2007..To prospectively determine the feasibility of imaging vascular volume fraction (VVF) and its therapeutic inhibition in mouse models of cancer with three-dimensional fluorescence molecular tomography (FMT)... - Real-time catheter molecular sensing of inflammation in proteolytically active atherosclerosisFarouc A Jaffer
MGH CMIR, 149 13th St, Room 5406, Boston, MA 02129, USA
Circulation 118:1802-9. 2008..To enable intravascular detection of inflammation in atherosclerosis, we developed a near-infrared fluorescence (NIRF) catheter-based strategy to sense cysteine protease activity during vascular catheterization... - A submillimeter resolution fluorescence molecular imaging system for small animal imagingEdward E Graves
Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA
Med Phys 30:901-11. 2003..Finally, the in vivo imaging capacity is showcased. It is expected that the large dataset collected can enable superior imaging of molecular probes in vivo and improve quantification of fluorescence signatures... - Free-space fluorescence molecular tomography utilizing 360 degrees geometry projectionsNikolaos Deliolanis
Laboratory for Biooptics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA
Opt Lett 32:382-4. 2007..Reconstruction feasibility using 36 projections in 10 degrees steps is demonstrated in mice... - Progress on multimodal molecular / anatomical intravascular imaging of coronary vessels combining near infrared fluorescence and ultrasoundGeorgios Mallas
Cardiovascular Research Center and Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
Conf Proc IEEE Eng Med Biol Soc 2011:1117-20. 2011..We include some results and models that showcase the potential power of this kind of hybrid imaging... - Fluorescence tomography and magnetic resonance imaging of myocardial macrophage infiltration in infarcted myocardium in vivoDavid E Sosnovik
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
Circulation 115:1384-91. 2007.... - Intravascular near-infrared fluorescence molecular imaging of atherosclerosis: toward coronary arterial visualization of biologically high-risk plaquesMarcella A Calfon
Massachusetts General Hospital, Cardiovascular Research Center and Cardiology Division, Boston, Massachusetts 02114, USA
J Biomed Opt 15:011107. 2010.... - Fluorescent protein tomography scanner for small animal imagingGiannis Zacharakis
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
IEEE Trans Med Imaging 24:878-85. 2005..We conclude that the method could be applied in tomographic imaging of fluorescent proteins for in vivo targeting of different diseases and abnormalities... - Accuracy of fluorescent tomography in the presence of heterogeneities: study of the normalized Born ratioAntoine Soubret
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
IEEE Trans Med Imaging 24:1377-86. 2005..Findings are further contrasted to images obtained with the standard Born expansion and with a normalized approach that divides the fluorescent field with excitation measurements through a homogeneous medium... - Improving quantification of intravascular fluorescence imaging using structural informationGeorgios Mallas
Department of Electrical and Computer Engineering, Communications and Digital Signal Processing Research Center, 409 Dana Building, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA
Phys Med Biol 57:6395-406. 2012..The findings herein point to quantification improvements when employing hybrid iNIRF, with possible implications to the clinical detection of high-risk plaques or blood vessel theranostics... - Hybrid FMT-CT imaging of amyloid-beta plaques in a murine Alzheimer's disease modelDamon Hyde
Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA
Neuroimage 44:1304-11. 2009..The results point to FMT-CT as an essential tool for in vivo study of neurodegenerative disease in animal models and potentially humans... - Imaging of molecular probe activity with Born-normalized fluorescence optical projection tomographyClaudio Vinegoni
Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, 185 Cambridge Street, Boston, Massachusetts 02114, USA
Opt Lett 35:1088-90. 2010.... - Complete-angle projection diffuse optical tomography by use of early photonsGordon M Turner
Laboratory for Bio-optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA
Opt Lett 30:409-11. 2005..The appropriateness of reconstruction algorithms similar to those employed in x-ray computed tomography are showcased, and suggestions for model improvements are provided... - Use of gene expression profiling to direct in vivo molecular imaging of lung cancerJan Grimm
Center for Molecular Imaging Research and Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA
Proc Natl Acad Sci U S A 102:14404-9. 2005..It also highlights the feasibility of using gene expression profiling to identify molecular targets for imaging lung cancer... - Inflammation in atherosclerosis: visualizing matrix metalloproteinase action in macrophages in vivoJun o Deguchi
Donald W Reynolds Cardiovascular Clinical Research Center, Harvard Medical School, Boston, Massachusetts, USA
Circulation 114:55-62. 2006..Matrix metalloproteinases (MMPs) in inflamed atherosclerotic plaques may contribute to extracellular matrix remodeling and the onset of acute thrombotic complications... - Imaging cathepsin B up-regulation in HT-1080 tumor models using fluorescence-mediated molecular tomography (FMT)Vasilis Ntziachristos
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown 02129-2060, USA
Acad Radiol 9:S323-5. 2002 - Performance dependence of hybrid x-ray computed tomography/fluorescence molecular tomography on the optical forward problemDamon Hyde
Department of Electrical and Computer Engineering, Northeastern University, 360 Huntington Ave, Boston, Massachusetts 02115, USA
J Opt Soc Am A Opt Image Sci Vis 26:919-23. 2009..Our results demonstrate that the use of generically known parameters provides near optimal performance, even when parameter mismatch remains... - In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomographyClaudio Vinegoni
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th Street, Charlestown, Massachusetts 02129, USA
Nat Methods 5:45-7. 2008..We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time... - Performance of the red-shifted fluorescent proteins in deep-tissue molecular imaging applicationsNikolaos C Deliolanis
Massachusetts General Hospital and Harvard Medical School, Center for Molecular Imaging Research, Laboratory for Bio optics and Molecular Imaging, Charlestown, Massachusetts 02139, USA
J Biomed Opt 13:044008. 2008.... - Two-dimensional intravascular near-infrared fluorescence molecular imaging of inflammation in atherosclerosis and stent-induced vascular injuryFarouc A Jaffer
Cardiovascular Research Center and Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
J Am Coll Cardiol 57:2516-26. 2011..This study sought to develop a 2-dimensional (2D) intravascular near-infrared fluorescence (NIRF) imaging strategy for investigation of arterial inflammation in coronary-sized vessels... - Singular-value analysis and optimization of experimental parameters in fluorescence molecular tomographyEdward E Graves
Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Room 5404, Charlestown, Massachusetts 02129, USA
J Opt Soc Am A Opt Image Sci Vis 21:231-41. 2004.... - Fluorescence molecular tomography in the presence of background fluorescenceAntoine Soubret
Laboratory for Bio-optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
Phys Med Biol 51:3983-4001. 2006..We show that the data pre-processing with subtraction schemes can improve image quality and quantification when non-specific background florescence is present... - Complete angle small animal fluorescence imaging with early-arriving photonsMark Niedre
Department of Electrical and Computer Engineering, Northeastern University, Boston, MA 02115, USA
Conf Proc IEEE Eng Med Biol Soc 2009:6331-4. 2009..High-fidelity image reconstruction using 72 projections in 5-degree steps using early-arriving photons is also demonstrated... - Shedding light onto live molecular targetsRalph Weissleder
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts, USA
Nat Med 9:123-8. 2003 - Time-resolved imaging of optical coefficients through murine chest cavitiesMark J Niedre
Massachusetts General Hospital, Harvard Medical School, Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Charlestown, Massachusetts 02129, USA
J Biomed Opt 11:064017. 2006..Photon transmission images through mouse chest cavities are further obtained at different time gates to visualize the spatial variation observed and confirm the optical coefficient patterns calculated... - Intra-arterial catheter for simultaneous microstructural and molecular imaging in vivoHongki Yoo
Wellman Center for Photomedicine, Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts, USA
Nat Med 17:1680-4. 2011.... - Noncontact optical tomography of turbid mediaRalf B Schulz
Laboratory for Bio-optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th Street, Suite 5209, Charlestown, Massachusetts 02129, USA
Opt Lett 28:1701-3. 2003..This approach offers significant experimental simplicity and yields high-information-content datasets. The performance of this novel tomographic approach is demonstrated with experimental reconstructions of phantoms... - Steady-state blood volume measurements in experimental tumors with different angiogenic burdens a study in miceChristoph Bremer
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Bldg 149, 13th St, 5406, Charlestown, MA 02129, USA
Radiology 226:214-20. 2003..CONCLUSION: Measurements of tumoral VVF at high-resolution MR imaging with long-circulating iron oxide are feasible and correlate with angiogenic burden in experimental tumor models... - Probing rat brain oxygenation with near-infrared spectroscopy (NIRS) and magnetic resonance imaging (MRI)Yu Chen
Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA
Adv Exp Med Biol 510:199-204. 2003 - In vivo imaging of proteolytic activity in atherosclerosisJiqiu Chen
Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston, USA
Circulation 105:2766-71. 2002..On the basis of the hypothesis that the inflammatory response and proteolysis lead to plaque rupture, we have examined the role of cathepsin B as a model proteolytic enzyme... - Projection access order in algebraic reconstruction technique for diffuse optical tomographyXavier Intes
Department of Biophysics and Biochemistry, University of Pennsylvania, Philadelphia 19104, USA
Phys Med Biol 47:N1-10. 2002..We show that by selecting proper projection access order, the convergence speed can be significantly improved when ART is used to perform DOT. Moreover, low-contrast detection is improved... - Optimization of 360 degrees projection fluorescence molecular tomographyTobias Lasser
Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, 5406 Charlestown, MA 02129, USA
Med Image Anal 11:389-99. 2007..We further discuss how these results can be employed to improve the performance of existing FMT systems and guide the design of new systems... - Multispectral photoacoustic imaging of fluorochromes in small animalsDaniel Razansky
Center for Molecular Imaging Research, Massachusetts General Hospital, MA 02129, USA
Opt Lett 32:2891-3. 2007..The suggested method is well suited for enhancing visualization of functional and molecular information in vivo and longitudinally... - High throughput magnetic resonance imaging for evaluating targeted nanoparticle probesDagmar Högemann
Center for Molecular Imaging Research, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
Bioconjug Chem 13:116-21. 2002..The technology should greatly accelerate the development of target-specific or cell-specific MR contrast agents... - Optical-based molecular imaging: contrast agents and potential medical applicationsChristoph Bremer
Department of Clinical Radiology, University of Munster, Albert Schweitzer Strasse 33, 48129 Munster, Germany
Eur Radiol 13:231-43. 2003.... - Iterative boundary method for diffuse optical tomographyJorge Ripoll
Institute for Electronic Structure and Laser, Foundation for Research and Technology Hellas, P O Box 1527, 71110 Heraklion, Greece
J Opt Soc Am A Opt Image Sci Vis 20:1103-10. 2003..Our observations are also contrasted with exact solutions. We furthermore investigate optimal implementation parameters and show that a second-order approximation is appropriate for most in vivo investigations... - Free-space propagation of diffuse light: theory and experimentsJorge Ripoll
Institute for Electronic Structure and Laser, Foundation for Research and Technology-Hellas, P.O. Box 1527, 71110 Heraklion, Greece
Phys Rev Lett 91:103901. 2003..The implications of this new formulation in the context of optical tomography in turbid media are discussed... - Experimental fluorescence tomography of tissues with noncontact measurementsRalf B Schulz
Department of Medical Physics in Radiology, German Cancer Research Center DKFZ, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
IEEE Trans Med Imaging 23:492-500. 2004..In this paper, we describe the experimental implementation of this novel concept and demonstrate capacity to perform small animal imaging... - Noncontact optical imaging in mice with full angular coverage and automatic surface extractionHeiko Meyer
Institute of Electronic Structure and Laser, Foundation for Research and Technology Hellas, Heraklion Crete, Greece
Appl Opt 46:3617-27. 2007..Finally, the potential use of this setup for in vivo small animal imaging and its impact on biomedical research is discussed... - Image analysis for assessing molecular activity changes in time-dependent geometriesKostas Marias
Medical Vision Laboratory, Department of Engineering Science, Oxford University, Oxford OX1 3PJ, UK
IEEE Trans Med Imaging 24:894-900. 2005..The method can account for differences in the positioning and compression of small animals and can be extended to three-dimensional as well as to other imaging modalities... - Three-dimensional in vivo imaging of green fluorescent protein-expressing T cells in mice with noncontact fluorescence molecular tomographyAnikitos Garofalakis
Institute of Electronic Structure and Laser, Foundation for Research and Techinology Hellas, Heraklion Crete, Greece
Mol Imaging 6:96-107. 2007..The technique was used to image and reconstruct fluorescence originating from 32 x 10(6) T cells in the thymus and 3 x 10(5) T cells in the spleen... - Validation of in vivo fluorochrome concentrations measured using fluorescence molecular tomographyEdward E Graves
Stanford University School of Medicine, Department of Radiation Oncology, Stanford, California 94305, USA
J Biomed Opt 10:44019. 2005..These findings demonstrate the in vivo quantitative accuracy of fluorescence tomography, and encourage further development of this imaging modality as well as application of FMT in molecular imaging studies using fluorescent reporters... - Effects of background fluorescence in fluorescence molecular tomographyMelisa Gao
Harvard-MIT Division of Health Sciences, Technology Biomedical Optics Summer Institute, 77 Massachusetts Avenue, E25-519, Cambridge, Massachusetts 02139, USA
Appl Opt 44:5468-74. 2005..Subtraction methods thus appear to be a promising route for adaptively correcting nonspecific background fluorochrome distribution... - Optical imaging of spontaneous breast tumors using protease sensing 'smart' optical probesChristoph Bremer
Department of Clinical Radiology, University of Muenster, Germany
Invest Radiol 40:321-7. 2005..The objective of this study was to determine if spontaneous breast cancer lesions can be detected by fluorescence reflectance imaging (FRI) and fluorescence mediated tomography (FMT) using protease-sensing optical probes... - Quantitative point source photoacoustic inversion formulas for scattering and absorbing mediaJorge Ripoll
Institute for Electronic Structure and Laser - Foundation for Research and Technology-Hellas, Heraklion, Greece
Phys Rev E Stat Nonlin Soft Matter Phys 71:031912. 2005..The proposed system could retrieve absorption information using photoacoustic measurements, and use these data to more accurately describe the fluorescence problem and improve reconstruction fidelity... - Experimental determination of photon propagation in highly absorbing and scattering mediaJorge Ripoll
Institute of Electronic Structure and Laser, Foundation for Research and Technology Hellas, Heraklion, Crete, Greece
J Opt Soc Am A Opt Image Sci Vis 22:546-51. 2005.... - Registration of 3-D CT and 2-D flat images of mouse via affine transformationZheng Xia
State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310027, China
IEEE Trans Inf Technol Biomed 12:569-78. 2008..The visualization of the alignment of the 3-D FMT and CT image through 2-D registration shows promising results... - Small-animal optical imagingVasilis Ntziachristos
J Biomed Opt 13:011001. 2008
Research Grants
- Hybrid optical-ultrasound scanner for cancer imagingVasilis Ntziachristos; Fiscal Year: 2005..abstract_text> ..
- ADVANCES IN OPTICS FOR BIOTECHNOLOGY, MEDICINEVasilis Ntziachristos; Fiscal Year: 2005..Aim 4: To promote interaction between the invited scientists and participating students, to support education and understanding of the multi-disciplinary field of bio-optics and associated applications and to enable collaborations. ..
- Whole body tomography of fluorescent proteins in vivoVasilis Ntziachristos; Fiscal Year: 2007....
- Hybrid Complete Protection Flourescence Molecular Tomography and X-ray CTRalph Weissleder; Fiscal Year: 2007..We hypothesize that the proposed developments will yield a practical and highly efficient system that can become a method of choice in many preclinical studies involving in-vivo imaging of entire animals. ..