Vasilis Ntziachristos

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi Looking and listening to light: the evolution of whole-body photonic imaging
    Vasilis Ntziachristos
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School CNY149, 13th Street 5406, Charlestown, Massachusetts 02129, USA
    Nat Biotechnol 23:313-20. 2005
  2. pmc Probing physiology and molecular function using optical imaging: applications to breast cancer
    V Ntziachristos
    Department of Bioengineering, University of Pennsylvania, Philadelphia, USA
    Breast Cancer Res 3:41-6. 2001
  3. pmc Visualization of antitumor treatment by means of fluorescence molecular tomography with an annexin V-Cy5.5 conjugate
    Vasilis Ntziachristos
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:12294-9. 2004
  4. ncbi Fluorescence imaging with near-infrared light: new technological advances that enable in vivo molecular imaging
    Vasilis Ntziachristos
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th Street 5406, Charlestown MA 02129 2060, USA
    Eur Radiol 13:195-208. 2003
  5. ncbi In vivo tomographic imaging of near-infrared fluorescent probes
    Vasilis Ntziachristos
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Bldg 149 13th Street 5406, Charlestown, MA 02129 2060, USA
    Mol Imaging 1:82-8. 2002
  6. ncbi Planar fluorescence imaging using normalized data
    Vasilis Ntziachristos
    Massachusetts General Hospital and Harvard Medical School, Center for Molecular Imaging Research, Laboratory for Biooptics and Molecular Imaging, Charlestown, Massachusetts 02129, USA
    J Biomed Opt 10:064007. 2005
  7. ncbi Fluorescence molecular tomography resolves protease activity in vivo
    Vasilis Ntziachristos
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts, USA
    Nat Med 8:757-60. 2002
  8. pmc MRI-guided diffuse optical spectroscopy of malignant and benign breast lesions
    Vasilis Ntziachristos
    Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104 6059, USA
    Neoplasia 4:347-54. 2002
  9. ncbi Fluorescence molecular imaging
    Vasilis Ntziachristos
    Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    Annu Rev Biomed Eng 8:1-33. 2006
  10. ncbi Charge-coupled-device based scanner for tomography of fluorescent near-infrared probes in turbid media
    Vasilis Ntziachristos
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown 02129, USA
    Med Phys 29:803-9. 2002

Research Grants

  1. Hybrid optical-ultrasound scanner for cancer imaging
    Vasilis Ntziachristos; Fiscal Year: 2005
  2. ADVANCES IN OPTICS FOR BIOTECHNOLOGY, MEDICINE
    Vasilis Ntziachristos; Fiscal Year: 2005
  3. Whole body tomography of fluorescent proteins in vivo
    Vasilis Ntziachristos; Fiscal Year: 2007
  4. Hybrid Complete Protection Flourescence Molecular Tomography and X-ray CT
    Ralph Weissleder; Fiscal Year: 2007

Collaborators

Detail Information

Publications77

  1. ncbi Looking and listening to light: the evolution of whole-body photonic imaging
    Vasilis Ntziachristos
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School CNY149, 13th Street 5406, Charlestown, Massachusetts 02129, USA
    Nat Biotechnol 23:313-20. 2005
    ..For photonic imaging to fully realize its potential, however, further progress will be needed in refining optical inversion methods and data acquisition techniques...
  2. pmc Probing physiology and molecular function using optical imaging: applications to breast cancer
    V Ntziachristos
    Department of Bioengineering, University of Pennsylvania, Philadelphia, USA
    Breast Cancer Res 3:41-6. 2001
    ..These features could enhance diagnostic accuracy, lower the current state-of-the-art detection limits, and play a vital role in therapeutic strategy and monitoring...
  3. pmc Visualization of antitumor treatment by means of fluorescence molecular tomography with an annexin V-Cy5.5 conjugate
    Vasilis Ntziachristos
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:12294-9. 2004
    ..Tomographic approaches are found essential for small-animal optical imaging and are potentially well suited for clinical drug development and monitoring...
  4. ncbi Fluorescence imaging with near-infrared light: new technological advances that enable in vivo molecular imaging
    Vasilis Ntziachristos
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th Street 5406, Charlestown MA 02129 2060, USA
    Eur Radiol 13:195-208. 2003
    ..We describe theoretical specifics, and we provide insight into its in vivo capacity and the sensitivity achieved. Finally, we discuss its clinical feasibility...
  5. ncbi In vivo tomographic imaging of near-infrared fluorescent probes
    Vasilis Ntziachristos
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Bldg 149 13th Street 5406, Charlestown, MA 02129 2060, USA
    Mol Imaging 1:82-8. 2002
    ..This validation of FMT with FRI demonstrated the spatial congruence of fluorochrome activation as determined by the two techniques...
  6. ncbi Planar fluorescence imaging using normalized data
    Vasilis Ntziachristos
    Massachusetts General Hospital and Harvard Medical School, Center for Molecular Imaging Research, Laboratory for Biooptics and Molecular Imaging, Charlestown, Massachusetts 02129, USA
    J Biomed Opt 10:064007. 2005
    ..Normalized planar imaging retains implementation simplicity and could be used to improve on standard fluorescence reflectance imaging and as a simplified alternative to the more integrated and accurate tomographic methods...
  7. ncbi Fluorescence molecular tomography resolves protease activity in vivo
    Vasilis Ntziachristos
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts, USA
    Nat Med 8:757-60. 2002
    ....
  8. pmc MRI-guided diffuse optical spectroscopy of malignant and benign breast lesions
    Vasilis Ntziachristos
    Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104 6059, USA
    Neoplasia 4:347-54. 2002
    ..It is foreseen that the technique can play a major role in functional activation studies of brain and muscle as well...
  9. ncbi Fluorescence molecular imaging
    Vasilis Ntziachristos
    Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    Annu Rev Biomed Eng 8:1-33. 2006
    ..Focus is given to small-animal imaging. However, while a broad spectrum of fluorescence reporter technologies and imaging methods are outlined, as necessary for biomedical research, and clinical translation as well...
  10. ncbi Charge-coupled-device based scanner for tomography of fluorescent near-infrared probes in turbid media
    Vasilis Ntziachristos
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown 02129, USA
    Med Phys 29:803-9. 2002
    ..These measurements delineate detection and reconstruction characteristics associated with imaging of novel classes of fluorescent probes developed for in vivo molecular and functional probing of tissues...
  11. ncbi Diffuse optical tomography of highly heterogeneous media
    V Ntziachristos
    Department of Bioengineering, University of Pennsylvania, Philadelphia 19104, USA
    IEEE Trans Med Imaging 20:470-8. 2001
    ..To eliminate the artifacts and improve the overall image reconstruction, we apply a data-correction algorithm that yields superior reconstruction results and is virtually independent of the degree of the background heterogeneity...
  12. pmc Early photon tomography allows fluorescence detection of lung carcinomas and disease progression in mice in vivo
    Mark J Niedre
    Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    Proc Natl Acad Sci U S A 105:19126-31. 2008
    ..The imaging fidelity demonstrated underscores a method that can use a wide range of fluorescent probes to accurately visualize cellular- and molecular-level events in whole animals in vivo...
  13. ncbi Quantitative analysis of chemotherapeutic effects in tumors using in vivo staining and correlative histology
    Heung Kook Choi
    Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    Cell Oncol 27:183-90. 2005
    ..To microscopically analyze the chemotherapeutic response of tumors using in vivo staining based on an annexinV-Cy5.5 probe and independently asses their apoptotic count using quantitative histological analysis...
  14. pmc Mapping molecular agents distributions in whole mice hearts using born-normalized optical projection tomography
    Claudio Vinegoni
    Center for System Biology, Massachusetts General Hospital and Harvard Medical School, Richard B Simches Research Center, Boston, Massachusetts, United States of America
    PLoS ONE 7:e34427. 2012
    ..We observed extensive monocyte recruitment within and around the infarcted areas and discovered that monocytes were also extensively recruited into non-ischemic myocardium, beyond that of injured tissue, such as the septum...
  15. ncbi In-vivo lung cancer imaging in mice using 360 degrees free-space fluorescence molecular tomography
    Nikolaos Deliolanis
    Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown 02129, USA
    Conf Proc IEEE Eng Med Biol Soc 1:2370-2. 2006
    ..Experimental data on imaging lung cancer are presented. Overall, this imaging approach can offer unprecedented imaging performance in fluorescence molecular tomography of small animals...
  16. ncbi Tomographic fluorescence mapping of tumor targets
    Xavier Montet
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
    Cancer Res 65:6330-6. 2005
    ..FMT measurements can be done serially, with short imaging times and within the same live animal. The described method should be valuable for rapidly profiling biological phenomena in vivo...
  17. pmc Transillumination fluorescence imaging in mice using biocompatible upconverting nanoparticles
    Claudio Vinegoni
    Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Opt Lett 34:2566-8. 2009
    ..The method holds great promise for artifact-free whole-body visualization of optical molecular probes...
  18. ncbi A statistical approach to inverting the Born ratio
    Damon Hyde
    Electrical and Computer Engineering Department, Northeastern University, Boston, MA 02115, USA
    IEEE Trans Med Imaging 26:893-905. 2007
    ..Results are presented for three experimental scenarios: phantom data with a homogeneous background, phantoms implanted within a small animal, and in vivo data using an exogenous probe...
  19. pmc Real-time catheter molecular sensing of inflammation in proteolytically active atherosclerosis
    Farouc A Jaffer
    MGH CMIR, 149 13th St, Room 5406, Boston, MA 02129, USA
    Circulation 118:1802-9. 2008
    ..To enable intravascular detection of inflammation in atherosclerosis, we developed a near-infrared fluorescence (NIRF) catheter-based strategy to sense cysteine protease activity during vascular catheterization...
  20. pmc Optical imaging of apoptosis as a biomarker of tumor response to chemotherapy
    Eyk A Schellenberger
    Center for Molecular Imaging Research, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Neoplasia 5:187-92. 2003
    ..The method maybe used to image pharmacologic responses in other animal models and, potentially, may permit the clinical imaging of apoptosis with noninvasive or minimally invasive instrumentation...
  21. ncbi Hybrid photoacoustic fluorescence molecular tomography using finite-element-based inversion
    Daniel Razansky
    Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA
    Med Phys 34:4293-301. 2007
    ..The potential promise of the suggested method is experimentally verified on tissue-mimicking fluorescent phantoms, where improvements in the quality of FMT reconstructions are observed when imaging at the presence of a large absorber...
  22. doi Surface Reconstruction for free-space 360 degrees fluorescence molecular tomography and the effects of animal motion
    Tobias Lasser
    Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hosiptal and Harvard Medical School, Charlestown, MA 02129, USA
    IEEE Trans Med Imaging 27:188-94. 2008
    ..We then employ this methodology to characterize the animal movement of anaesthetized animals. We find that the effects of animal movement on the FMT reconstructed image were within system resolution limits (approximately 0.07 cm)...
  23. pmc Volumetric tomography of fluorescent proteins through small animals in vivo
    Giannis Zacharakis
    Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Harvard Medical School, Charlestown, MA 02129, USA
    Proc Natl Acad Sci U S A 102:18252-7. 2005
    ..This technology can significantly improve imaging capacity over the current state of the art and should find wide in vivo imaging applications in drug discovery, immunology, and cancer research...
  24. pmc Indocyanine green enables near-infrared fluorescence imaging of lipid-rich, inflamed atherosclerotic plaques
    Claudio Vinegoni
    Center for Molecular Imaging Research, Massachusetts General Hospital, Boston, MA 02114, USA
    Sci Transl Med 3:84ra45. 2011
    ..The atheroma-targeting capability of ICG has the potential to accelerate the clinical development of NIRF molecular imaging of high-risk plaques in humans...
  25. doi Data specific spatially varying regularization for multimodal fluorescence molecular tomography
    Damon Hyde
    Computational Radiology Laboratory, Children s Hospital Boston, Harvard Medical School, Boston, MA 02115 USA
    IEEE Trans Med Imaging 29:365-74. 2010
    ..Results are presented for both simulated and experimental data sets, and show significant improvements in image quality as compared to traditional regularization techniques...
  26. ncbi Inversion with early photons
    Gordon M Turner
    Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, CNY 149 13th Street 5406, Charlestown, Massachusetts 02129, USA
    Med Phys 34:1405-11. 2007
    ..We find that all methods perform adequately given appropriate regularization parameters, and that an experimentally measured photon weight function yields superior results over two approximate weights that have been previously used...
  27. pmc Normalized Born ratio for fluorescence optical projection tomography
    Claudio Vinegoni
    Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Opt Lett 34:319-21. 2009
    ..We use the algorithm to three-dimensionally reconstruct and characterize a fluorescein isothiocyanate containing absorptive phantom and an infarcted mouse heart previously injected with a fluorescent molecular probe...
  28. doi Visualization of pulmonary inflammation using noninvasive fluorescence molecular imaging
    Jodi Haller
    Center for Molecular Imaging Research Laboratory For Bio optics and Molecular Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, USA
    J Appl Physiol 104:795-802. 2008
    ..The data presented herein serve to further evince fluorescence molecular imaging as a technology highly appropriate for the biomedical laboratory...
  29. ncbi Normalized transillumination of fluorescent proteins in small animals
    Giannis Zacharakis
    Harvard Medical School, MA, USA
    Mol Imaging 5:153-9. 2006
    ..Due to the balance achieved between simplicity and accuracy, normalized transillumination approaches could serve as an important alternative molecular imaging method...
  30. doi Comparison of fluorescence tomographic imaging in mice with early-arriving and quasi-continuous-wave photons
    Mark Niedre
    Department of Electrical and Computer Engineering, Northeastern University, Boston, Massachusetts 02115, USA
    Opt Lett 35:369-71. 2010
    ....
  31. ncbi Tomographic fluorescence imaging of tumor vascular volume in mice
    Xavier Montet
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th St, Room 5403, Charlestown, MA 02129, USA
    Radiology 242:751-8. 2007
    ..To prospectively determine the feasibility of imaging vascular volume fraction (VVF) and its therapeutic inhibition in mouse models of cancer with three-dimensional fluorescence molecular tomography (FMT)...
  32. ncbi A submillimeter resolution fluorescence molecular imaging system for small animal imaging
    Edward E Graves
    Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA
    Med Phys 30:901-11. 2003
    ..Finally, the in vivo imaging capacity is showcased. It is expected that the large dataset collected can enable superior imaging of molecular probes in vivo and improve quantification of fluorescence signatures...
  33. ncbi Free-space fluorescence molecular tomography utilizing 360 degrees geometry projections
    Nikolaos Deliolanis
    Laboratory for Biooptics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA
    Opt Lett 32:382-4. 2007
    ..Reconstruction feasibility using 36 projections in 10 degrees steps is demonstrated in mice...
  34. doi Progress on multimodal molecular / anatomical intravascular imaging of coronary vessels combining near infrared fluorescence and ultrasound
    Georgios Mallas
    Cardiovascular Research Center and Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
    Conf Proc IEEE Eng Med Biol Soc 2011:1117-20. 2011
    ..We include some results and models that showcase the potential power of this kind of hybrid imaging...
  35. ncbi Fluorescence tomography and magnetic resonance imaging of myocardial macrophage infiltration in infarcted myocardium in vivo
    David E Sosnovik
    Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
    Circulation 115:1384-91. 2007
    ....
  36. pmc Intravascular near-infrared fluorescence molecular imaging of atherosclerosis: toward coronary arterial visualization of biologically high-risk plaques
    Marcella A Calfon
    Massachusetts General Hospital, Cardiovascular Research Center and Cardiology Division, Boston, Massachusetts 02114, USA
    J Biomed Opt 15:011107. 2010
    ....
  37. ncbi Accuracy of fluorescent tomography in the presence of heterogeneities: study of the normalized Born ratio
    Antoine Soubret
    Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    IEEE Trans Med Imaging 24:1377-86. 2005
    ..Findings are further contrasted to images obtained with the standard Born expansion and with a normalized approach that divides the fluorescent field with excitation measurements through a homogeneous medium...
  38. ncbi Fluorescent protein tomography scanner for small animal imaging
    Giannis Zacharakis
    Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    IEEE Trans Med Imaging 24:878-85. 2005
    ..We conclude that the method could be applied in tomographic imaging of fluorescent proteins for in vivo targeting of different diseases and abnormalities...
  39. pmc Improving quantification of intravascular fluorescence imaging using structural information
    Georgios Mallas
    Department of Electrical and Computer Engineering, Communications and Digital Signal Processing Research Center, 409 Dana Building, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA
    Phys Med Biol 57:6395-406. 2012
    ..The findings herein point to quantification improvements when employing hybrid iNIRF, with possible implications to the clinical detection of high-risk plaques or blood vessel theranostics...
  40. ncbi Complete-angle projection diffuse optical tomography by use of early photons
    Gordon M Turner
    Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA
    Opt Lett 30:409-11. 2005
    ..The appropriateness of reconstruction algorithms similar to those employed in x-ray computed tomography are showcased, and suggestions for model improvements are provided...
  41. ncbi Performance dependence of hybrid x-ray computed tomography/fluorescence molecular tomography on the optical forward problem
    Damon Hyde
    Department of Electrical and Computer Engineering, Northeastern University, 360 Huntington Ave, Boston, Massachusetts 02115, USA
    J Opt Soc Am A Opt Image Sci Vis 26:919-23. 2009
    ..Our results demonstrate that the use of generically known parameters provides near optimal performance, even when parameter mismatch remains...
  42. ncbi Imaging cathepsin B up-regulation in HT-1080 tumor models using fluorescence-mediated molecular tomography (FMT)
    Vasilis Ntziachristos
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown 02129 2060, USA
    Acad Radiol 9:S323-5. 2002
  43. pmc Use of gene expression profiling to direct in vivo molecular imaging of lung cancer
    Jan Grimm
    Center for Molecular Imaging Research and Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA
    Proc Natl Acad Sci U S A 102:14404-9. 2005
    ..It also highlights the feasibility of using gene expression profiling to identify molecular targets for imaging lung cancer...
  44. pmc Imaging of molecular probe activity with Born-normalized fluorescence optical projection tomography
    Claudio Vinegoni
    Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Opt Lett 35:1088-90. 2010
    ....
  45. doi Hybrid FMT-CT imaging of amyloid-beta plaques in a murine Alzheimer's disease model
    Damon Hyde
    Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA
    Neuroimage 44:1304-11. 2009
    ..The results point to FMT-CT as an essential tool for in vivo study of neurodegenerative disease in animal models and potentially humans...
  46. ncbi Inflammation in atherosclerosis: visualizing matrix metalloproteinase action in macrophages in vivo
    Jun o Deguchi
    Donald W Reynolds Cardiovascular Clinical Research Center, Harvard Medical School, Boston, Massachusetts, USA
    Circulation 114:55-62. 2006
    ..Matrix metalloproteinases (MMPs) in inflamed atherosclerotic plaques may contribute to extracellular matrix remodeling and the onset of acute thrombotic complications...
  47. ncbi In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography
    Claudio Vinegoni
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th Street, Charlestown, Massachusetts 02129, USA
    Nat Methods 5:45-7. 2008
    ..We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time...
  48. pmc Two-dimensional intravascular near-infrared fluorescence molecular imaging of inflammation in atherosclerosis and stent-induced vascular injury
    Farouc A Jaffer
    Cardiovascular Research Center and Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
    J Am Coll Cardiol 57:2516-26. 2011
    ..This study sought to develop a 2-dimensional (2D) intravascular near-infrared fluorescence (NIRF) imaging strategy for investigation of arterial inflammation in coronary-sized vessels...
  49. pmc Performance of the red-shifted fluorescent proteins in deep-tissue molecular imaging applications
    Nikolaos C Deliolanis
    Massachusetts General Hospital and Harvard Medical School, Center for Molecular Imaging Research, Laboratory for Bio optics and Molecular Imaging, Charlestown, Massachusetts 02139, USA
    J Biomed Opt 13:044008. 2008
    ....
  50. ncbi Singular-value analysis and optimization of experimental parameters in fluorescence molecular tomography
    Edward E Graves
    Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Room 5404, Charlestown, Massachusetts 02129, USA
    J Opt Soc Am A Opt Image Sci Vis 21:231-41. 2004
    ....
  51. ncbi Fluorescence molecular tomography in the presence of background fluorescence
    Antoine Soubret
    Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    Phys Med Biol 51:3983-4001. 2006
    ..We show that the data pre-processing with subtraction schemes can improve image quality and quantification when non-specific background florescence is present...
  52. doi Complete angle small animal fluorescence imaging with early-arriving photons
    Mark Niedre
    Department of Electrical and Computer Engineering, Northeastern University, Boston, MA 02115, USA
    Conf Proc IEEE Eng Med Biol Soc 2009:6331-4. 2009
    ..High-fidelity image reconstruction using 72 projections in 5-degree steps using early-arriving photons is also demonstrated...
  53. ncbi Shedding light onto live molecular targets
    Ralph Weissleder
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts, USA
    Nat Med 9:123-8. 2003
  54. ncbi Time-resolved imaging of optical coefficients through murine chest cavities
    Mark J Niedre
    Massachusetts General Hospital, Harvard Medical School, Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Charlestown, Massachusetts 02129, USA
    J Biomed Opt 11:064017. 2006
    ..Photon transmission images through mouse chest cavities are further obtained at different time gates to visualize the spatial variation observed and confirm the optical coefficient patterns calculated...
  55. pmc Intra-arterial catheter for simultaneous microstructural and molecular imaging in vivo
    Hongki Yoo
    Wellman Center for Photomedicine, Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts, USA
    Nat Med 17:1680-4. 2011
    ....
  56. ncbi Noncontact optical tomography of turbid media
    Ralf B Schulz
    Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th Street, Suite 5209, Charlestown, Massachusetts 02129, USA
    Opt Lett 28:1701-3. 2003
    ..This approach offers significant experimental simplicity and yields high-information-content datasets. The performance of this novel tomographic approach is demonstrated with experimental reconstructions of phantoms...
  57. ncbi Steady-state blood volume measurements in experimental tumors with different angiogenic burdens a study in mice
    Christoph Bremer
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Bldg 149, 13th St, 5406, Charlestown, MA 02129, USA
    Radiology 226:214-20. 2003
    ....
  58. ncbi Probing rat brain oxygenation with near-infrared spectroscopy (NIRS) and magnetic resonance imaging (MRI)
    Yu Chen
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Adv Exp Med Biol 510:199-204. 2003
  59. ncbi In vivo imaging of proteolytic activity in atherosclerosis
    Jiqiu Chen
    Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston, USA
    Circulation 105:2766-71. 2002
    ..On the basis of the hypothesis that the inflammatory response and proteolysis lead to plaque rupture, we have examined the role of cathepsin B as a model proteolytic enzyme...
  60. ncbi Projection access order in algebraic reconstruction technique for diffuse optical tomography
    Xavier Intes
    Department of Biophysics and Biochemistry, University of Pennsylvania, Philadelphia 19104, USA
    Phys Med Biol 47:N1-10. 2002
    ..We show that by selecting proper projection access order, the convergence speed can be significantly improved when ART is used to perform DOT. Moreover, low-contrast detection is improved...
  61. ncbi Optimization of 360 degrees projection fluorescence molecular tomography
    Tobias Lasser
    Laboratory for Bio optics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, 5406 Charlestown, MA 02129, USA
    Med Image Anal 11:389-99. 2007
    ..We further discuss how these results can be employed to improve the performance of existing FMT systems and guide the design of new systems...
  62. ncbi Multispectral photoacoustic imaging of fluorochromes in small animals
    Daniel Razansky
    Center for Molecular Imaging Research, Massachusetts General Hospital, MA 02129, USA
    Opt Lett 32:2891-3. 2007
    ..The suggested method is well suited for enhancing visualization of functional and molecular information in vivo and longitudinally...
  63. ncbi High throughput magnetic resonance imaging for evaluating targeted nanoparticle probes
    Dagmar Högemann
    Center for Molecular Imaging Research, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    Bioconjug Chem 13:116-21. 2002
    ..The technology should greatly accelerate the development of target-specific or cell-specific MR contrast agents...
  64. ncbi Optical-based molecular imaging: contrast agents and potential medical applications
    Christoph Bremer
    Department of Clinical Radiology, University of Munster, Albert Schweitzer Strasse 33, 48129 Munster, Germany
    Eur Radiol 13:231-43. 2003
    ....
  65. ncbi Iterative boundary method for diffuse optical tomography
    Jorge Ripoll
    Institute for Electronic Structure and Laser, Foundation for Research and Technology Hellas, P O Box 1527, 71110 Heraklion, Greece
    J Opt Soc Am A Opt Image Sci Vis 20:1103-10. 2003
    ..Our observations are also contrasted with exact solutions. We furthermore investigate optimal implementation parameters and show that a second-order approximation is appropriate for most in vivo investigations...
  66. ncbi Free-space propagation of diffuse light: theory and experiments
    Jorge Ripoll
    Institute for Electronic Structure and Laser, Foundation for Research and Technology Hellas, P O Box 1527, 71110 Heraklion, Greece
    Phys Rev Lett 91:103901. 2003
    ..The implications of this new formulation in the context of optical tomography in turbid media are discussed...
  67. ncbi Experimental fluorescence tomography of tissues with noncontact measurements
    Ralf B Schulz
    Department of Medical Physics in Radiology, German Cancer Research Center DKFZ, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    IEEE Trans Med Imaging 23:492-500. 2004
    ..In this paper, we describe the experimental implementation of this novel concept and demonstrate capacity to perform small animal imaging...
  68. ncbi Noncontact optical imaging in mice with full angular coverage and automatic surface extraction
    Heiko Meyer
    Institute of Electronic Structure and Laser, Foundation for Research and Technology Hellas, Heraklion Crete, Greece
    Appl Opt 46:3617-27. 2007
    ..Finally, the potential use of this setup for in vivo small animal imaging and its impact on biomedical research is discussed...
  69. ncbi Image analysis for assessing molecular activity changes in time-dependent geometries
    Kostas Marias
    Medical Vision Laboratory, Department of Engineering Science, Oxford University, Oxford OX1 3PJ, UK
    IEEE Trans Med Imaging 24:894-900. 2005
    ..The method can account for differences in the positioning and compression of small animals and can be extended to three-dimensional as well as to other imaging modalities...
  70. ncbi Three-dimensional in vivo imaging of green fluorescent protein-expressing T cells in mice with noncontact fluorescence molecular tomography
    Anikitos Garofalakis
    Institute of Electronic Structure and Laser, Foundation for Research and Techinology Hellas, Heraklion Crete, Greece
    Mol Imaging 6:96-107. 2007
    ..The technique was used to image and reconstruct fluorescence originating from 32 x 10(6) T cells in the thymus and 3 x 10(5) T cells in the spleen...
  71. ncbi Validation of in vivo fluorochrome concentrations measured using fluorescence molecular tomography
    Edward E Graves
    Stanford University School of Medicine, Department of Radiation Oncology, Stanford, California 94305, USA
    J Biomed Opt 10:44019. 2005
    ..These findings demonstrate the in vivo quantitative accuracy of fluorescence tomography, and encourage further development of this imaging modality as well as application of FMT in molecular imaging studies using fluorescent reporters...
  72. ncbi Effects of background fluorescence in fluorescence molecular tomography
    Melisa Gao
    Harvard MIT Division of Health Sciences, Technology Biomedical Optics Summer Institute, 77 Massachusetts Avenue, E25 519, Cambridge, Massachusetts 02139, USA
    Appl Opt 44:5468-74. 2005
    ..Subtraction methods thus appear to be a promising route for adaptively correcting nonspecific background fluorochrome distribution...
  73. ncbi Optical imaging of spontaneous breast tumors using protease sensing 'smart' optical probes
    Christoph Bremer
    Department of Clinical Radiology, University of Muenster, Germany
    Invest Radiol 40:321-7. 2005
    ..The objective of this study was to determine if spontaneous breast cancer lesions can be detected by fluorescence reflectance imaging (FRI) and fluorescence mediated tomography (FMT) using protease-sensing optical probes...
  74. ncbi Quantitative point source photoacoustic inversion formulas for scattering and absorbing media
    Jorge Ripoll
    Institute for Electronic Structure and Laser Foundation for Research and Technology Hellas, Heraklion, Greece
    Phys Rev E Stat Nonlin Soft Matter Phys 71:031912. 2005
    ..The proposed system could retrieve absorption information using photoacoustic measurements, and use these data to more accurately describe the fluorescence problem and improve reconstruction fidelity...
  75. ncbi Experimental determination of photon propagation in highly absorbing and scattering media
    Jorge Ripoll
    Institute of Electronic Structure and Laser, Foundation for Research and Technology Hellas, Heraklion, Crete, Greece
    J Opt Soc Am A Opt Image Sci Vis 22:546-51. 2005
    ....
  76. doi Registration of 3-D CT and 2-D flat images of mouse via affine transformation
    Zheng Xia
    State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310027, China
    IEEE Trans Inf Technol Biomed 12:569-78. 2008
    ..The visualization of the alignment of the 3-D FMT and CT image through 2-D registration shows promising results...
  77. ncbi Small-animal optical imaging
    Vasilis Ntziachristos
    J Biomed Opt 13:011001. 2008

Research Grants4

  1. Hybrid optical-ultrasound scanner for cancer imaging
    Vasilis Ntziachristos; Fiscal Year: 2005
    ..abstract_text> ..
  2. ADVANCES IN OPTICS FOR BIOTECHNOLOGY, MEDICINE
    Vasilis Ntziachristos; Fiscal Year: 2005
    ..Aim 4: To promote interaction between the invited scientists and participating students, to support education and understanding of the multi-disciplinary field of bio-optics and associated applications and to enable collaborations. ..
  3. Whole body tomography of fluorescent proteins in vivo
    Vasilis Ntziachristos; Fiscal Year: 2007
    ....
  4. Hybrid Complete Protection Flourescence Molecular Tomography and X-ray CT
    Ralph Weissleder; Fiscal Year: 2007
    ..We hypothesize that the proposed developments will yield a practical and highly efficient system that can become a method of choice in many preclinical studies involving in-vivo imaging of entire animals. ..