Affiliation: Harvard Medical School
- Biochemical, electron microscopic and immunohistological observations of cationic detergent-extracted cells: detection and improved preservation of microextensions and ultramicroextensionsF Nakamura
Laboratory of Environmental Biochemistry, Department of Environmental Biology, Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
BMC Cell Biol 2:10. 2001..By contrast, formaldehyde is generally used for immunofluorescence light microscopy, but few studies have been concerned with the loss of microextensions...
- Silica gel high-performance liquid chromatography for the determination of cross-links in elastinF Nakamura
Laboratory of Molecular Technology of Animal Products, Faculty of Agriculture, Tohoku University, Sendai, Japan
J Chromatogr Sci 29:217-20. 1991..47 and 6.44 +/- 0.51 (mean +/- S.D., n = 10) mg/g dry weight, respectively, in bovine ligamentum nuchae. Preparations of cross-links are carried out by means of a preparative column (240 mm x 10 mm), using the same solvent system...
- Protein phosphatase 2C inactivates F-actin binding of human platelet moesinA Hishiya
Department of Environmental Biology, Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
J Biol Chem 274:26705-12. 1999..In vivo, regulation of these phosphatase activities are likely to influence dynamic interactions between the actin cytoskeleton and membrane constituents linked to moesin...
- Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositidesF Nakamura
Laboratory of Environmental Biochemistry, Department of Environmental Biology, Graduate School of Agricultural Sciences, Tohoku University, Sendai 981 8555, Japan
Mol Biol Cell 10:2669-85. 1999..Dual regulation by both mechanisms may be important for proper cellular control of moesin-mediated linkages between the actin cytoskeleton and the plasma membrane...
- Oxodesmosine and isooxodesmosine, candidates of oxidative metabolic intermediates of pyridinium cross-links in elastinH Umeda
Department of Applied Bioorganic Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
Arch Biochem Biophys 385:209-19. 2001..Pyridinium cross-links, such as DES and IDE, may be oxidatively metabolized by ROS, further changing to dihydrooxopyridine cross-links such as OXD and IOXD, respectively...