Research Topics
Genomes and Genes | Andrew W MurraySummaryAffiliation: Harvard University Country: USA Publications
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Publications
Don't make me mad, Bub!Andrew W Murray
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
Dev Cell 22:1123-5. 2012..In this issue of Developmental Cell, Suijkerbuijk et al. (2012) provide evidence that Bub1 has duplicated and diverged many times during eukaryotic evolution, dividing the functions of its ancestor between the two duplicated copies...
Journey to the centre of the cellA W Murray
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Nat Cell Biol 2:E130-1. 2000....- Recycling the cell cycle: cyclins revisitedAndrew W Murray
Department of Molecular and Cellular Biology, Biological Laboratories, Harvard University, Cambridge, MA 02138, USA
Cell 116:221-34. 2004..I discuss how a cyclin/Cdk-based engine could have evolved to assume control of the cell cycle from other, older protein kinases... - Can sequencing shed light on cell cycling?A W Murray
Center for Genomics Research, Harvard University, Cambridge, Massachusetts 02138, USA
Nature 409:844-6. 2001..Disappointingly, we discovered a few novel cyclins and no new Cdks or components of the spindle checkpoint, and could shed little light on the organization of the cell cycle... - Whither genomics?A W Murray
Department of Physiology, University of California San Francisco, CA 94143, USA
Genome Biol 1:COMMENT003. 2000.... - Anaphase inactivation of the spindle checkpointWilliam J Palframan
Department of Molecular and Cellular Biology, Biological Laboratories, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
Science 313:680-4. 2006..Furthermore, expression of Mps1 in anaphase, or repression of the APC in anaphase, reactivates the spindle checkpoint. This APC-Mps1 feedback circuit allows cells to irreversibly inactivate the checkpoint during anaphase... - Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosisErin L Barnhart
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
Mol Biol Cell 22:2448-57. 2011..We conclude that cells need the normal Mad2:Mad1 ratio to respond to chromosomes that are not under tension... - Mad2 and Mad3 cooperate to arrest budding yeast in mitosisDerek T C Lau
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
Curr Biol 22:180-90. 2012..Despite numerous studies, we still do not understand how the checkpoint proteins coordinate with each other to inhibit APC(Cdc20) activity... - Budding yeast mitotic chromosomes have an intrinsic bias to biorient on the spindleVahan B Indjeian
Department of Molecular and Cellular Biology, Harvard University, Massachusetts 02138, USA
Curr Biol 17:1837-46. 2007..After the spindle has been depolymerized and allowed to reform, budding yeast sgo1 mutants fail to biorient their sister chromatids and die as cells divide... - A small-molecule inhibitor of Mps1 blocks the spindle-checkpoint response to a lack of tension on mitotic chromosomesRussell K Dorer
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Curr Biol 15:1070-6. 2005..Our results demonstrate that Mps1 can be exploited as a target and that inhibiting the tension-sensitive branch of the spindle checkpoint may be a way of selectively killing cancer cells that display chromosomal instability... - The conserved protein kinase Ipl1 regulates microtubule binding to kinetochores in budding yeastS Biggins
Department of Physiology, University of California, San Francisco, California 94143 0444, USA
Genes Dev 13:532-44. 1999..Because aurora2 has been implicated in oncogenesis, defects in kinetochore function may contribute to genetic instability in human tumors... 
An in vitro assay for Cdc20-dependent mitotic anaphase-promoting complex activity from budding yeastScott C Schuyler
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA
Methods Mol Biol 545:271-85. 2009..Here we outline a quantitative in vitro mitotic APC(Cdc20) assay that makes use of a highly active form of the APC that is purified from budding yeast cells arrested in mitosis...- Recruiting a microtubule-binding complex to DNA directs chromosome segregation in budding yeastSoni Lacefield
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Nat Cell Biol 11:1116-20. 2009..We conclude that tethering a single kinetochore protein to DNA triggers assembly of the complex structure that directs mitotic chromosome segregation... - Cdc28 activates exit from mitosis in budding yeastA D Rudner
Department of Physiology, University of California, San Francisco, California 94143 0444, USA
J Cell Biol 149:1361-76. 2000..The defects of CDC28-VF suggest that Cdc28 activity is required to induce the metaphase to anaphase transition and initiate the transition from anaphase to G1 in budding yeast... - A novel yeast screen for mitotic arrest mutants identifies DOC1, a new gene involved in cyclin proteolysisL H Hwang
Department of Physiology, University of California, San Francisco 94143 0444, USA
Mol Biol Cell 8:1877-87. 1997..Cdc26 associates in vivo with Doc1, Cdc16, Cdc23, and Cdc27. In addition, the majority of Doc1 cosediments at 20S with Cdc27 in a sucrose gradient, indicating that Cdc26 and Doc1 are components of the anaphase promoting complex... - High-resolution mutation mapping reveals parallel experimental evolution in yeastAyellet V Segrè
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA
PLoS Biol 4:e256. 2006..Our results show an example of parallel adaptation caused by mutations in the same gene... - The centromeric protein Sgo1 is required to sense lack of tension on mitotic chromosomesVahan B Indjeian
Department of Molecular and Cellular Biology, Biological Laboratories, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
Science 307:130-3. 2005.... - The spindle checkpoint rescues the meiotic segregation of chromosomes whose crossovers are far from the centromereSoni Lacefield
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA
Nat Genet 39:1273-7. 2007..The tether partially rescues the segregation of chromosomes that lack crossovers... - Lesions in many different spindle components activate the spindle checkpoint in the budding yeast Saccharomyces cerevisiaeK G Hardwick
Department of Physiology, University of California, San Francisco, California 94143 0444, USA
Genetics 152:509-18. 1999..In contrast, the cell cycle arrest caused by mutations that induce DNA damage (cdc13), inactivate the cyclin proteolysis machinery (cdc16 and cdc23), or arrest cells in anaphase (cdc15) is independent of the spindle checkpoint... - Identification of xenopus CENP-A and an associated centromeric DNA repeatNathaniel S Edwards
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
Mol Biol Cell 16:1800-10. 2005..Fluorescent in situ hybridization with Fcr1 probes stained most centromeres in cultured cells. By staining lampbrush chromosomes, we specifically identified the 11 (of 18) chromosomes that stain consistently with Fcr1 probes... - The speed of evolution and maintenance of variation in asexual populationsMichael M Desai
Department of Physics, Harvard University, Cambridge, MA 02138, USA
Curr Biol 17:385-94. 2007..We focus on the effects of such multiple mutations... - Mutation rates across budding yeast chromosome VI are correlated with replication timingGregory I Lang
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA
Genome Biol Evol 3:799-811. 2011..This model is supported by the observation that eliminating translesion synthesis decreases this variation... - Activation of the budding yeast spindle assembly checkpoint without mitotic spindle disruptionK G Hardwick
Department of Physiology, University of California, San Francisco, CA 94143 0444, USA
Science 273:953-6. 1996..Ectopic activation of cell-cycle checkpoints might be used to exploit the differences in checkpoint status between normal and tumor cells and thus improve the selectivity of chemotherapy... - Budding yeast Cdc20: a target of the spindle checkpointL H Hwang
Department of Physiology, University of California at San Francisco, San Francisco, CA 94143 0444, USA
Science 279:1041-4. 1998..Mutants in Cdc20 that were resistant to the spindle checkpoint no longer bound Mad proteins, suggesting that Cdc20 is the target of the spindle checkpoint... - Time-lapse microscopy reveals unique roles for kinesins during anaphase in budding yeastA F Straight
Department of Physiology, School of Medicine, University of California San Francisco, San Francisco, California 94143, USA
J Cell Biol 143:687-94. 1998..The kip3Delta and kip1Delta mutants affect the duration of anaphase, but cin8Delta does not... - Phosphorylation by Cdc28 activates the Cdc20-dependent activity of the anaphase-promoting complexA D Rudner
Department of Physiology, University of California, San Francisco, California 94143 0444, USA
J Cell Biol 149:1377-90. 2000..We show that, like cdc28 mutants, cdc5 mutants affect APC phosphorylation in vivo. However, although Cdc5 can phosphorylate Cdc16 and Cdc27 in vitro, this in vitro phosphorylation does not occur on in vivo sites of phosphorylation... - The Xenopus chromokinesin Xkid is essential for metaphase chromosome alignment and must be degraded to allow anaphase chromosome movementH Funabiki
Department of Physiology, University of California, San Francisco 94143, USA
Cell 102:411-24. 2000..We propose that Xkid provides the metaphase force that pushes chromosome arms toward the equator of the spindle and that its destruction is needed for anaphase chromosome movement... - Association of spindle assembly checkpoint component XMAD2 with unattached kinetochoresR H Chen
Department of Physiology, University of California, San Francisco, 94143, USA Chapel Hill, NC 27599, USA
Science 274:242-6. 1996..This study furthers understanding of the mechanism of cell cycle checkpoints in metazoa and provides a marker for studying the role of the spindle assembly checkpoint in the genetic instability of tumors... - Lack of tension at kinetochores activates the spindle checkpoint in budding yeastB M Stern
Department of Molecular and Cell Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
Curr Biol 11:1462-7. 2001..Because the spindle checkpoint is activated in these cells, we conclude that the absence of tension at the yeast kinetochore is sufficient to activate the spindle checkpoint in mitosis... - A brief history of errorAndrew W Murray
Harvard University, Molecular and Cellular Biology, 52 Oxford Street, Northwest Science Building, Cambridge, Massachusetts 02138, USA
Nat Cell Biol 13:1178-82. 2011..This perspective will present a brief history summarizing what we know about the checkpoint, and a list of questions we must answer before we understand it... - Positive-feedback loops as a flexible biological moduleNicholas T Ingolia
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Curr Biol 17:668-77. 2007..Bistable behavior can result from positive feedback, but feedback loops can have other roles in signal transduction as well... - Estimating the per-base-pair mutation rate in the yeast Saccharomyces cerevisiaeGregory I Lang
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Genetics 178:67-82. 2008..44x10(-10) at CAN1) and we propose a definition for the effective target size of genes (the probability that a mutation inactivates the gene) that acknowledges that the mutation rate is nonuniform across the genome... - Members of the NAP/SET family of proteins interact specifically with B-type cyclinsD R Kellogg
Department of Physiology, University of California, San Francisco 94143 0444, USA
J Cell Biol 130:661-73. 1995..This protein is the Xenopus homolog of the human SET protein, which was previously identified as part of a putative oncogenic fusion protein (Von Lindern et al., 1992)... - Genetic selection of peptide inhibitors of biological pathwaysT C Norman
Department of Physiology, University of California, San Francisco, CA 94143 0444, USA
Science 285:591-5. 1999.... - Improved use of a public good selects for the evolution of undifferentiated multicellularityJohn H Koschwanez
FAS Center for Systems Biology and Department of Molecular and Cellular Biology, Harvard University, Cambridge, United States
elife 2:e00367. 2013..Our study shows that combining rational design with experimental evolution can help evaluate hypotheses about evolutionary strategies. DOI:http://dx.doi.org/10.7554/eLife.00367.001... - Sucrose utilization in budding yeast as a model for the origin of undifferentiated multicellularityJohn H Koschwanez
FAS Center for Systems Biology, Harvard University, Cambridge, Massachusetts, United States of America
PLoS Biol 9:e1001122. 2011..We propose that the prior use of public goods led to selection for the incomplete cell separation that first produced multicellularity... - Ploidy controls the success of mutators and nature of mutations during budding yeast evolutionDawn A Thompson
Molecular and Cellular Biology Department, 16 Divinity Ave, Harvard University, Cambridge, MA 02138, USA
Curr Biol 16:1581-90. 2006..CONCLUSIONS: We conclude that the advantage of mutators depends on ploidy and that diploid mutators can give rise to beneficial mutations that are inaccessible to nonmutators and haploid mutators... - The ups and downs of modeling the cell cycleNicholas T Ingolia
Department of Molecular and Cellular Biology, Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138, USA
Curr Biol 14:R771-7. 2004..These include asking how the various elaborations of the basic oscillator affect the robustness of the system and how cells monitor their size and use this information to control the cell cycle... - Rapid expansion and functional divergence of subtelomeric gene families in yeastsChris A Brown
Faculty of Arts and Sciences Center for Systems Biology, Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA
Curr Biol 20:895-903. 2010..However, most genome sequences do not contain assembled subtelomeric sequences, and, as a result, subtelomeres are often overlooked in comparative genomics... - Spo13 protects meiotic cohesin at centromeres in meiosis IMarion A Shonn
Department of Molecular and Cell Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Genes Dev 16:1659-71. 2002..Overexpressing the mitotic cohesin, Scc1/Mcd1, does not substitute for Rec8, suggesting that the combined actions of Spo13 and Rec8 are important for preventing sister centromere separation in meiosis I... - NAP1 acts with Clb1 to perform mitotic functions and to suppress polar bud growth in budding yeastD R Kellogg
Department of Physiology, University of California, San Francisco 94143 0444, USA
J Cell Biol 130:675-85. 1995.... - The spindle checkpoint of budding yeast depends on a tight complex between the Mad1 and Mad2 proteinsR H Chen
Department of Physiology, University of California, San Francisco, San Francisco, California 94143 0444, USA
Mol Biol Cell 10:2607-18. 1999..Deletion and mutational analysis of both proteins indicate that association of Mad2p with Mad1p is critical for checkpoint function and for hyperphosphorylation of Mad1p... - Selective sweeps in growing microbial coloniesKirill S Korolev
FAS Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA
Phys Biol 9:026008. 2012..Spatial sector patterns therefore provide an alternative fitness assay to the commonly used liquid culture fitness assays... - Spindle checkpoint component Mad2 contributes to biorientation of homologous chromosomesMarion A Shonn
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02130, USA
Curr Biol 13:1979-84. 2003.... - Experimental evolution of mating discrimination in budding yeastJun Yi Leu
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Ave, Room 3000, Cambridge, Massachusetts 02138, USA
Curr Biol 16:280-6. 2006..Genetic analysis indicates that multiple mutations have accumulated to produce the altered mating preference. Our results show that subtle details of mating behavior can play an important role in the evolution of reproductive isolation... - Sister chromatid cohesion in mitosisS Biggins
513 Parnassus Avenue, Box 0444, Department of Physiology, University of California, San Francisco, San Francisco, California 94143 0444, USA
Curr Opin Genet Dev 9:230-6. 1999..Proteins that induce and regulate the separation of sister chromatids have also been recently identified. (This review is an updated version of one that was published in Current Opinion in Cell Biology 1998, 10:769-775.).. - Mad1p, a phosphoprotein component of the spindle assembly checkpoint in budding yeastK G Hardwick
Department of Physiology, University of California, San Francisco 94143 0444, USA
J Cell Biol 131:709-20. 1995..We discuss the possible functions of Mad1p at this cell cycle checkpoint... - Signal transduction. History mattersNicholas T Ingolia
Department of Molecular and Cellular Biology and Bauer Center for Genomics Research, Harvard University, Cambridge, MA 02138, USA
Science 297:948-9. 2002 - The spindle assembly checkpointA D Rudner
Department of Physiology, Box 0444, University of California at San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143 0444, USA
Curr Opin Cell Biol 8:773-80. 1996..Checkpoint components reside on kinetochores of chromosomes and show changes in phosphorylation and localization as cells proceed through mitosis. Adaptation to prolonged checkpoint arrest can occur by inhibitory phosphorylation of Cdc2... - Genes involved in sister chromatid separation and segregation in the budding yeast Saccharomyces cerevisiaeS Biggins
Department of Physiology, University of California, San Francisco, 94143, USA
Genetics 159:453-70. 2001..We also report an initial characterization of phenotypes associated with the SMT3/SUMO gene and the isolation of WSS1, a high-copy smt3 suppressor... - Mutation of YCS4, a budding yeast condensin subunit, affects mitotic and nonmitotic chromosome behaviorNeedhi Bhalla
Department of Cell Biology and Physiology, University of California, San Francisco, San Francisco, California 94143, USA
Mol Biol Cell 13:632-45. 2002..Taken together, our data suggest that there are mitotic as well as nonmitotic chromosomal abnormalities associated with loss of condensin function in budding yeast...
Research Grants
- FEEDBACK CONTROL OF THE CELL CYCLEAndrew W Murray; Fiscal Year: 2010..This project uses brewer's yeast to understand how cells normally keep track of their chromosomes and how mutations can wreck the tracking machinery. ..
- SISTER CHROMATID LINKAGE AND SEPARATIONAndrew Murray; Fiscal Year: 2000..This information will be directly relevant to understanding the generation of aneuploidy seen in Down syndrome and tumor progression. ..
- Modular biology: experiment, theory and computationAndrew Murray; Fiscal Year: 2007..abstract_text> ..
- FEEDBACK CONTROL OF THE CELL CYCLEAndrew Murray; Fiscal Year: 2007..These inhibitors will be useful as research tools in organisms that lack sophisticated genetics, will identify new components of the checkpoint, and mavrenresent a novel class of chemotherapeutic agents. ..
- Sixth International Conference on Systems Biology (ICSB 2005)Andrew Murray; Fiscal Year: 2005..The speakers and contributors will include experimental biologists, chemists, computer scientists, physicists, engineers, and mathematicians. ..
- SISTER CHROMATID LINKAGE AND SEPARATIONAndrew Murray; Fiscal Year: 2004..This information will be directly relevant to understanding how aneuploidy is generated in Down syndrome and tumor progression. ..