Danesh Moazed

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. doi request reprint Chromatin: a tail of repression
    Danesh Moazed
    Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Curr Biol 23:R456-9. 2013
  2. pmc Small RNAs in transcriptional gene silencing and genome defence
    Danesh Moazed
    Howard Hughes Medical Institute, and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 457:413-20. 2009
  3. pmc Reconstitution of heterochromatin-dependent transcriptional gene silencing
    Aaron Johnson
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 35:769-81. 2009
  4. pmc The methyltransferase activity of Clr4Suv39h triggers RNAi independently of histone H3K9 methylation
    Erica L Gerace
    Department of Cell Biology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 39:360-72. 2010
  5. pmc HP1 proteins form distinct complexes and mediate heterochromatic gene silencing by nonoverlapping mechanisms
    Mohammad R Motamedi
    Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 32:778-90. 2008
  6. pmc Sir3-nucleosome interactions in spreading of silent chromatin in Saccharomyces cerevisiae
    Johannes R Buchberger
    Howard Hughes Medical Institute, Harvard Medical School, 240 Longwood Ave, Boston, MA 02115, USA
    Mol Cell Biol 28:6903-18. 2008
  7. pmc Dicer-independent primal RNAs trigger RNAi and heterochromatin formation
    Mario Halic
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 140:504-16. 2010
  8. ncbi request reprint Coupling of double-stranded RNA synthesis and siRNA generation in fission yeast RNAi
    Serafin U Colmenares
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 27:449-61. 2007
  9. pmc siRNA-mediated heterochromatin establishment requires HP1 and is associated with antisense transcription
    Tetsushi Iida
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 31:178-89. 2008
  10. ncbi request reprint Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs
    Mohammad R Motamedi
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 119:789-802. 2004

Collaborators

Detail Information

Publications43

  1. doi request reprint Chromatin: a tail of repression
    Danesh Moazed
    Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Curr Biol 23:R456-9. 2013
    ..A recent study reports that fruit flies carrying histone H3 lysine 27 (H3K27) mutations have the same homeotic gene expression and developmental defects as mutations in the enzyme that trimethylates H3K27...
  2. pmc Small RNAs in transcriptional gene silencing and genome defence
    Danesh Moazed
    Howard Hughes Medical Institute, and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 457:413-20. 2009
    ....
  3. pmc Reconstitution of heterochromatin-dependent transcriptional gene silencing
    Aaron Johnson
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 35:769-81. 2009
    ..These results define a minimal set of components that mediate heterochromatic gene silencing and demonstrate distinct contributions for histone deacetylation and nucleosome binding in the silencing mechanism...
  4. pmc The methyltransferase activity of Clr4Suv39h triggers RNAi independently of histone H3K9 methylation
    Erica L Gerace
    Department of Cell Biology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 39:360-72. 2010
    ..These findings establish Rik1 as a key link between CLRC and RNAi and reveal distinct centromeric siRNA amplification mechanisms that depend on the Clr4 methyltransferase activity...
  5. pmc HP1 proteins form distinct complexes and mediate heterochromatic gene silencing by nonoverlapping mechanisms
    Mohammad R Motamedi
    Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 32:778-90. 2008
    ..Our findings reveal an unexpected role for Swi6 in RNAi-mediated gene silencing and suggest that different HP1 proteins ensure full heterochromatic gene silencing through largely nonoverlapping inhibitory mechanisms...
  6. pmc Sir3-nucleosome interactions in spreading of silent chromatin in Saccharomyces cerevisiae
    Johannes R Buchberger
    Howard Hughes Medical Institute, Harvard Medical School, 240 Longwood Ave, Boston, MA 02115, USA
    Mol Cell Biol 28:6903-18. 2008
    ..We propose a model for the spreading of the SIR complex along the chromatin fiber through the two distinct histone-binding domains in Sir3...
  7. pmc Dicer-independent primal RNAs trigger RNAi and heterochromatin formation
    Mario Halic
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 140:504-16. 2010
    ..Our results suggest that a transcriptome surveillance mechanism based on random association of RNA degradation products with Argonaute triggers siRNA amplification and heterochromatin assembly within DNA repeats...
  8. ncbi request reprint Coupling of double-stranded RNA synthesis and siRNA generation in fission yeast RNAi
    Serafin U Colmenares
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 27:449-61. 2007
    ..This coupling has implications for cis-restriction of siRNA-mediated heterochromatin assembly and for mechanisms that give rise to siRNA strand polarity...
  9. pmc siRNA-mediated heterochromatin establishment requires HP1 and is associated with antisense transcription
    Tetsushi Iida
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 31:178-89. 2008
    ..These results provide insights into the regulation of siRNA action and reveal roles for cis-dsRNA synthesis and HP1 in siRNA-mediated heterochromatin assembly...
  10. ncbi request reprint Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs
    Mohammad R Motamedi
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 119:789-802. 2004
    ..These findings reveal a physical and functional link between Rdp1 and RITS and suggest that noncoding RNAs provide a platform for siRNA-dependent localization of RNAi complexes to specific chromosome regions...
  11. pmc Role for perinuclear chromosome tethering in maintenance of genome stability
    Karim Mekhail
    Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 456:667-70. 2008
    ..Our results therefore reveal an ancient mechanism in which interactions between INM proteins and chromosomal proteins ensure genome stability...
  12. pmc Budding yeast silencing complexes and regulation of Sir2 activity by protein-protein interactions
    Jason C Tanny
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell Biol 24:6931-46. 2004
    ..Finally, we provide evidence that the different nicotinamide sensitivities of Sir2/Sir4 and RENT in vitro could contribute to locus-specific differences in how Sir2 activity is regulated in vivo...
  13. pmc Inhibition of homologous recombination by a cohesin-associated clamp complex recruited to the rDNA recombination enhancer
    Julie Huang
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 20:2887-901. 2006
    ....
  14. ncbi request reprint Role of the conserved Sir3-BAH domain in nucleosome binding and silent chromatin assembly
    Megumi Onishi
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 28:1015-28. 2007
    ..Together, these observations suggest that the SIR complex associates with an extended chromatin fiber through interactions with two different regions in the nucleosome...
  15. ncbi request reprint Tethering RITS to a nascent transcript initiates RNAi- and heterochromatin-dependent gene silencing
    Marc Buhler
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 125:873-86. 2006
    ..These findings support a model in which targeting of nascent transcripts by RITS mediates chromatin modifications and suggest that cotranscriptional processing events play a primary role in the silencing mechanism...
  16. pmc A nonhistone protein-protein interaction required for assembly of the SIR complex and silent chromatin
    Adam D Rudner
    Harvard Medical School, Department of Cell Biology, 240 Longwood Ave, Boston, MA 02115, USA
    Mol Cell Biol 25:4514-28. 2005
    ....
  17. ncbi request reprint Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast
    Shane M Buker
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 14:200-7. 2007
    ..Finally, we show that slicer activity is required for the siRNA-dependent association of Ago1 with chromatin and for the spreading of histone H3-K9 methylation...
  18. pmc TRAMP-mediated RNA surveillance prevents spurious entry of RNAs into the Schizosaccharomyces pombe siRNA pathway
    Marc Buhler
    Department of Cell Biology, 240 Longwood Avenue, Harvard Medical School, Boston, Massachusetts 02115 USA
    Nat Struct Mol Biol 15:1015-23. 2008
    ..Thus, Cid14 prevents certain abundant RNAs from becoming substrates for the RNAi machinery, thereby freeing the RNAi machinery to act on its proper targets...
  19. ncbi request reprint The replication fork block protein Fob1 functions as a negative regulator of the FEAR network
    Frank Stegmeier
    Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17 233, 40 Ames St, Cambridge, MA 02139, USA
    Curr Biol 14:467-80. 2004
    ..The Cdc14 early anaphase release (FEAR) network promotes activation of the phosphatase during early anaphase, whereas the mitotic exit network (MEN) activates Cdc14 during late stages of anaphase...
  20. ncbi request reprint Assembly of the SIR complex and its regulation by O-acetyl-ADP-ribose, a product of NAD-dependent histone deacetylation
    Gunn Guang Liou
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 121:515-27. 2005
    ..These results suggest that Sir2 activity modulates the assembly of the SIR complex through both histone deacetylation and AAR synthesis...
  21. ncbi request reprint RNAi-directed assembly of heterochromatin in fission yeast
    Andre Verdel
    Department of Cell Biology, LHRRB 517, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    FEBS Lett 579:5872-8. 2005
    ..These discoveries have given rise to a model in which small interfering RNA molecules act as specificity factors that initiate epigenetic chromatin modifications and double strand RNA synthesis at specific chromosome regions...
  22. ncbi request reprint A cullin E3 ubiquitin ligase complex associates with Rik1 and the Clr4 histone H3-K9 methyltransferase and is required for RNAi-mediated heterochromatin formation
    Eun Jin Erica Hong
    Department of Cell Biology, Harvard Medical School, Boston, Masachusetts 02115, USA
    RNA Biol 2:106-11. 2005
    ..Based on these results, we propose that the components of the Clr4-Rik1-Cul4 complex act concertedly at an early step in heterochromatin formation...
  23. ncbi request reprint S. pombe LSD1 homologs regulate heterochromatin propagation and euchromatic gene transcription
    Fei Lan
    Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 26:89-101. 2007
    ....
  24. ncbi request reprint RNAi-dependent and -independent RNA turnover mechanisms contribute to heterochromatic gene silencing
    Marc Buhler
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 129:707-21. 2007
    ..Our findings indicate that polyadenylation by a TRAMP-like complex contributes to robust silencing of heterochromatic genes in fission yeast via the recruitment of the exosome and/or the RNAi machinery...
  25. ncbi request reprint A model for step-wise assembly of heterochromatin in yeast
    Danesh Moazed
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Novartis Found Symp 259:48-56; discussion 56-62, 163-9. 2004
    ..A distinct Sir2 complex called RENT is required for silencing at rDNA. In contrast to telomeres and the mating type loci, Sir2 activity is not required for association of RENT with rDNA...
  26. ncbi request reprint Sister chromatid cohesion in silent chromatin: each sister to her own ring
    Julie Huang
    Department of Cell Biology, Harvard Medical School, Boston Massachusetts 02115, USA
    Genes Dev 20:132-7. 2006
  27. ncbi request reprint Structure of the coiled-coil dimerization motif of Sir4 and its interaction with Sir3
    Ju Fang Chang
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Structure 11:637-49. 2003
    ..The dual interactions of Sir4 with Sir3 and Sir2 suggest a physical basis for recruiting Sir3 to chromatin by virtue of its interactions with Sir4 and with deacetylated histones in chromatin...
  28. pmc Heterochromatin protein Sir3 induces contacts between the amino terminus of histone H4 and nucleosomal DNA
    Feng Wang
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 110:8495-500. 2013
    ....
  29. pmc Association of the RENT complex with nontranscribed and coding regions of rDNA and a regional requirement for the replication fork block protein Fob1 in rDNA silencing
    Julie Huang
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Genes Dev 17:2162-76. 2003
    ..Furthermore, the Fob1-dependent associations of Net1 and Sir2 with the recombination hotspot region strongly suggest that Sir2 acts directly at this region to carry out its inhibitory effect on rDNA recombination and accelerated aging...
  30. ncbi request reprint Acetylated histone tail peptides induce structural rearrangements in the RSC chromatin remodeling complex
    Georgios Skiniotis
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 282:20804-8. 2007
    ..Based on three-dimensional reconstructions, we propose a model for the interaction of RSC with acetylated nucleosomes...
  31. pmc 22G-RNAs in transposon silencing and centromere function
    Mario Halic
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 36:170-1. 2009
    ..Three recent papers (Gu et al., 2009; Claycomb et al., 2009; van Wolfswinkel et al., 2009) provide evidence that links a new class of small RNAs and Argonaute-associated complexes to centromere function and genome surveillance...
  32. ncbi request reprint Labeling and characterization of small RNAs associated with the RNA interference effector complex RITS
    Andre Verdel
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Methods Enzymol 392:297-307. 2005
    ..Here we review the affinity purification methods we use to isolate RITS and describe how to purify, detect, and analyze RNAs associated with this complex...
  33. ncbi request reprint Centromere assembly and propagation
    Corey A Morris
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 128:647-50. 2007
    ..Here, we review the possible epigenetic strategies that organisms ranging from yeast to human use to assemble and propagate active centromeres...
  34. ncbi request reprint A proteomic strategy for gaining insights into protein sumoylation in yeast
    Carilee Denison
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell Proteomics 4:246-54. 2005
    ..These data combine with recent works to further our understanding of the breadth and impact of protein sumoylation in a diverse array of biological processes...
  35. pmc RNAi in fission yeast finds new targets and new ways of targeting at the nuclear periphery
    Daniel Holoch
    Department of Cell Biology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Genes Dev 26:741-5. 2012
    ..Unexpectedly, the initial targeting of RNAi components to these genes does not require small RNA guides...
  36. pmc RNAi-mediated targeting of heterochromatin by the RITS complex
    Andre Verdel
    Department of Cell Biology, Harvard Medical School, Boston, MA02115, USA
    Science 303:672-6. 2004
    ..The results suggest a mechanism for the role of the RNAi machinery and small RNAs in targeting of heterochromatin complexes and epigenetic gene silencing at specific chromosomal loci...
  37. pmc Transposon silencing by piRNAs
    Mario Halic
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 138:1058-60. 2009
    ..2009) report that an HP1 family protein, Rhino, is required for piRNA generation and transposon silencing in Drosophila germline cells. The results provide a link between heterochromatin and piRNA-mediated genome defense...
  38. ncbi request reprint Recognition of acetylated proteins: lessons from an ancient family of enzymes
    Jason C Tanny
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Structure 10:1290-2. 2002
    ..The structure of a Sir2-like enzyme in complex with an acetylated peptide substrate has been solved, and provides the first glimpse into the mechanism of substrate recognition by this highly conserved family of enzymes...
  39. ncbi request reprint Association of the histone methyltransferase Set2 with RNA polymerase II plays a role in transcription elongation
    Jiaxu Li
    Department of Cell Biology, Harvard Medical School, 240 Longwood Ave, Boston, MA 02115 5730, USA
    J Biol Chem 277:49383-8. 2002
    ..Together, our results suggest that Set2 through association with the elongating form of RNA polymerase II plays an important role in transcription elongation...
  40. pmc Mechanisms for the inheritance of chromatin states
    Danesh Moazed
    Howard Hughes Medical Institute, Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 146:510-8. 2011
    ....
  41. pmc Steps in assembly of silent chromatin in yeast: Sir3-independent binding of a Sir2/Sir4 complex to silencers and role for Sir2-dependent deacetylation
    Georg J Hoppe
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 22:4167-80. 2002
    ..However, the binding of Sir2 to rDNA is independent of its histone deacetylase activity. Together, these results support a stepwise model for the assembly of silent chromatin domains in Saccharomyces cerevisiae...
  42. ncbi request reprint Transcription and RNAi in heterochromatic gene silencing
    Marc Buhler
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02445, USA
    Nat Struct Mol Biol 14:1041-8. 2007
    ..In this review, we discuss the roles of RNA and RNA turnover in mechanisms that mediate heterochromatin assembly and keep heterochromatic domains silent...
  43. pmc Histone demethylation and timely DNA replication
    Erica L Gerace
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 40:683-4. 2010
    ..In this issue of Molecular Cell, Black et al. (2010) uncover a conserved role for the JMJD2 family of histone demethylases in promoting replication within silent chromatin regions that contain histone H3 lysine 9 methylation and HP1...